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Citations for Jackson ImmunoResearch :
551 - 592 of 592 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... washed twice with PBSB by centrifugation for 5 min at 1200 rpm and then incubated for an additional hour with 50 µL of R-Phycoerythrin AffiniPure F(ab’)₂ Fragment Goat Anti-Human IgG (Jackson ImmunoResearch, Cat#109-116-170) at 250 ng/mL diluted in PBSB ...
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bioRxiv - Bioengineering 2021Quote: ... Approximately 200 RUs of VHH-Fcs (2 µg/mL) were captured on goat anti-human IgG surfaces (4000 RUs, Jackson ImmunoResearch, Cat#109-005-098) at a flow rate of 10 µL/min for 30 s ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then pelleted washed twice with PBSA and subsequently labeled with PE-conjugated goat anti-human IgG (1:100, Jackson ImmunoResearch Labs #109-115-098) and FITC-conjugated chicken anti-cMmyc (1:100 ...
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bioRxiv - Immunology 2021Quote: ... live cells expressing S delta 19aa were suspended in QSol Buffer (IntelliCyte Corporation) to which a cocktail of Fc-specific secondary antibodies was added: AF647 goat anti-human IgG (Jackson ImmunoResearch, Cat # 109-605-008), AF488 goat anti-human IgA (Jackson ImmunoResearch ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in 100 µL of FACS buffer containing secondary R-PE Goat Anti-Human IgG antibody diluted 1:100 (Jackson ImmunoResearch, cat #109-117-008), and incubated for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... Total IgG was measured following 6 washes and a 1-h incubation with 100 μL/well of 1:50,000 HRP-conjugated polyclonal goat anti-human IgG (Jackson ImmunoResearch Labs, West Grove, PA, USA). IgG3 was measured following 6 washes and a 1-h incubation with 100 μL/well 1:5,000 mouse anti-human biotin-conjugated IgG3 hinge (SouthernBiotech ...
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bioRxiv - Microbiology 2023Quote: ... We then washed cells three times in binding buffer and incubated them with a R-phycoerythrin (PE)-coupled goat anti-human F(ab’)2 fragment (Jackson ImmunoResearch Cat #: 109-116-098) in binding buffer for 30 minutes at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... wells were washed 6 times and 1:5000 dilution of horseradish peroxidase (HRP)-conjugated donkey anti-human IgG (FC specific; Jackson ImmunoResearch Laboratories, West Grove, PA) was added in blocking solution to each well and the plate was incubated for 1 hour at RT ...
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bioRxiv - Immunology 2023Quote: ... Grids were washed several times with PBS and incubated with 12-nm gold-labeled anti-human secondary antibody (Jackson ImmunoResearch, diluted 1:40 in PBS) for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and the SARS-CoV-2 RBD specific IgG signal was detected by incubating with horseradish peroxidase (HRP) conjugated - anti-human IgG (Jackson ImmunoResearch Labs, #109-036-098). Plates were then washed thoroughly and developed with o-phenylenediamine (OPD ...
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bioRxiv - Immunology 2024Quote: The IgG assay utilized a 1:100 dilution of goat anti-human IgG (Fc)-HRP in Conjugate Diluent (HRP-StabilPlus, Jackson Immunoresearch Laboratories, West Grove, PA) was added for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... The plates were washed three times with TBST-Ca2+ before incubating with a goat anti-human IgG conjugated with horseradish peroxidase (Jackson ImmunoResearch, Cat# 109-035-098). Bound TIM1-Fc(mono ...
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bioRxiv - Microbiology 2024Quote: ... Fluorescently labeled Alexa Fluor 647 and Alexa Fluor 488 human transferrin were purchased from Jackson ImmunoResearch (Cat. No. 009-600-050 and 009-540-050) and employed at a dilution of 1:100 ...
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bioRxiv - Bioengineering 2024Quote: ... 2mL 1% BSA prepared in DPBS supplemented with 5 µg/ml APC-conjugated Goat anti-Human IgG Fcγ (Jackson ImmunoResearch, Cat. No. 109-136-170) and 3 µg/ml Kappa-FITC Goat anti-Human antibody (Southern Biotechnology ...
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bioRxiv - Microbiology 2024Quote: ... Proteins of interest were detected with specific primary antibodies and secondary antibodies coupled to horseradish peroxidase (Jackson ImmunoResearch). Densitometry was measured with ImageJ (https://imagej.net).
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in RPMI medium + Pen/Strep + L-glutamine + 10% FBS and stimulated with polyclonal goat anti-human IgM (Jackson Immunoresearch Affinipure; 0.6-75 ug/mL), or equimolar concentrations of the Fab’2 fragments (Jackson Immunoresearch Affinipure ...
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bioRxiv - Cell Biology 2021Quote: External beads were stained for live-cell imaging by incubating macrophages with Cy2-conjugated anti-human IgG (1:100; Jackson ImmunoResearch Labs, West Grove, PA, USA) for 30 min on ice ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then washed with PBS and incubated with allophycocyanin (APC)-conjugated F(ab′)2 fragment donkey anti-human IgG antibody (Jackson ImmunoResearch Laboratories, cat# 709-136-149) at a final concentration of 62.5 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... goat anti-human serum IgA (α-chain specific) at 0.3 μg/mL or goat anti-human IgG (γ-chain specific) at 5 μg/mL (all from Jackson ImmunoResearch Laboratories, Inc., West Grove, PA). Microtiter plates were sealed and stored overnight at 2-8°C ...
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bioRxiv - Immunology 2023Quote: ... or unconjugated anti-human CCL19 (polyclonal, R&D AF361-SP, 1:200) that was detected with anti-goat IgG A488 (Jackson Immunoresearch 705-545-003, 1:1000) and unconjugated anti-human PDPN (Clone NZ-1.3 ...
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bioRxiv - Immunology 2023Quote: ... Bacteria were washed twice with filtered PBS and then resuspended in 0.2 µm-filtered solutions of appropriate secondary reagents (Alexa 647-anti-human IgG (Jackson ImmunoResearch, 1:100, 109-605-098, AB_2337889) and Brilliant Violet 421-anti-rabbit IgG (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... antibody responses were detected with biotin-conjugated goat anti-human IgG (1:1000 dilution) or IgA (1:500 dilution) secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) and visualized with streptavidin-conjugated SureLight® P3 (Columbia Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... Endogenous immunoglobulins were blocked by incubating tissue in Fab fragment goat anti-human IgG (H+L) (Jackson ImmunoResearch Labs, West Grove, PA, USA; 100 μg/ml) for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... for 1 hour and then incubated in the cold overnight with an anti-human IgG antibody at a 1:1000 dilution (#109-005-098, Jackson ImmunoResearch Labs, West Grove, Pennsylvania, USA). Blots were washed 3 times with 0.1% Tween 20 in TBS before incubating with a horseradish peroxidase-conjugated anti-goat secondary antibody at a 1:4000 dilution (#A16005 ...
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bioRxiv - Genetics 2020Quote: ... Cy5 or Alexa 647 secondary antibody pre-absorbed against a range of mammalian serum proteins (Jackson Immunoresearch and ThermoFisher). This protocol was modified for FISH as described (Radford and McKim ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins of interest were probed using the appropriate primary and Horse Radish Peroxidase (HRP)-conjugated secondary antibodies (Jackson Immunoresearch), detected using SuperSignal West Pico or Femto chemiluminescence substrates (Pierce) ...
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bioRxiv - Microbiology 2022Quote: ... Protein-primary antibody complexes were revealed with horseradish peroxidase-labeled donkey anti-rabbit antibodies (Jackson ImmunoResearch, West Grove, PA) and ECL detection reagents (Amersham ...
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bioRxiv - Biochemistry 2024Quote: ... The membrane was washed with TBT-Tween 0.1% and immunoreactive proteins were detected using anti-mouse IgG conjugated with peroxidase (1:10,000, Jackson ImmunoResearch) for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Proteins were visualized using peroxidase-coupled secondary antibodies (rabbit-POD, Sigma-Aldrich, A6154; mouse-POD, Jackson ImmunoResearch, 115-035-062) and an enhanced chemiluminescence system (Cell Signaling Technology).
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were detected using HRP-coupled secondary antibody (1:15,000 goat anti-rabbit, Jackson ImmunoResearch Labs 111-035-144, RRID:AB_2307391). Chemiluminescence was detected using Amersham ECL reagent (GE Healthcare).
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bioRxiv - Cell Biology 2024Quote: ... The protein fraction was precleared for 1 h with 50 μL of normal rabbit serum (Jackson ImmunoResearch, 011-000-120) and 50 μL of agarose A/G bead slurry (Pierce™ Protein A/G Agarose ...
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bioRxiv - Immunology 2021Quote: Ficolled and FACS-sorted (CD3-/CD14-/CD19+) human peripheral B cells were stimulated with either BCR cross-linking antibodies (αBCR; Jackson Immunoresearch, Cata No. 109-006-129, 10ug/mL) or CD40L (Enzo Life Sciences ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained for 30 minutes at 4°C with a fluorescently labeled secondary antibody – anti-human-IgG-F(ab’)2 (Jackson ImmunoResearch #109-606-097, used at 1:400) for Nivolumab and Durvalumab-stained samples ...
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bioRxiv - Biochemistry 2020Quote: ... washed three times with PBS-T and were subsequently treated for 30 min with anti-human Alexa 647–conjugated secondary antibody (Jackson ImmunoResearch; dilution: 1:200 in PBS-T + 2 % BSA) to immunostain the centromeres ...
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bioRxiv - Microbiology 2021Quote: ... Membrane-bound N proteins were detected with a rabbit polyclonal antibody (Novus) and a horseradish peroxidase-conjugated secondary antibody (Jackson Immunoresearch). Detection was carried out by chemoluminescence (Pierce ...
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bioRxiv - Neuroscience 2022Quote: ... Dishes were coated with 50 μg/ml GPC3coreUG protein mixed with 120 μg/ml anti-hFc (Jackson ImmunoResearch 109-005-098) for 30 min at 37°C and washed with PBS ...
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bioRxiv - Cell Biology 2024Quote: Immunoreactive proteins were detected using a horseradish-peroxidase-labeled secondary antibody for mouse IgG (1:10,000; Jackson ImmunoResearch, 115-035-003) or for anti-rabbit IgG (1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... Isotype matched IgG with identical protein concentration was used as a negative control (donkey anti-rabbit IgG; Jackson ImmunoResearch #711-005-152).
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bioRxiv - Cancer Biology 2020Quote: ... and the other Western blot analysis to locate the membrane protein band by streptavidin-conjugated with horseradish peroxidase (HRP) (Jackson ImmunoResearch Laboratories) and ECL substrate (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... immunofluorescence staining was performed on deparaffinized and rehydrated tissue sections using a rabbit anti-SARS-CoV-2-NP protein antibody together with an AF488-conjugated anti-rabbit IgG (Jackson ImmunoResearch, PA, USA). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... Dishes were coated with 50 μg/ml Fc or GPC3coreUG protein mixed with 120 μg/ml anti-hFc (Jackson ImmunoResearch 109-005-098) for 30 min at 37°C and washed with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... permeabilization and blocking of endogenous proteins were done with 0.5% Triton X-100 and 10% normal donkey serum (NDS, Jackson Immunoresearch, 017-000-121) in PBS for 30 min ...