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Citations for Jackson ImmunoResearch :
551 - 600 of 722 citations for IL 3 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... Brains were incubated in blocking buffer consisting of PTxwH with 3% normal donkey serum (Jackson Immunoresearch, West Grove, PA, USA) at 37°C overnight ...
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bioRxiv - Immunology 2019Quote: ... The slides were washed 3 times with 1X PBS followed by incubation with fluorescent dye-conjugated secondary antibodies (Jackson ImmunoResearch) at room temperature for 1hr ...
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bioRxiv - Neuroscience 2021Quote: ... brains were left rocking at 4°C for 1-3 nights in 0.1% PBS-T supplemented with blocking agent normal goat serum (Jackson ImmunoResearch) at a 1:20 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Kidney were incubated in blocking buffer consisting of PTxwH with 3% normal donkey serum (Jackson Immunoresearch, West Grove, PA, USA) at 37°C for 48 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed 3 × 10 min with PBST (1X PBS with 0.5% Triton X-100) and incubated in appropriate secondary antibodies (Jackson ImmunoResearch) conjugated to Alexa 488 ...
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bioRxiv - Neuroscience 2022Quote: ... and 36 µg rabbit anti-HA-tag (Cell Signalling Technology #3724) followed by fluorescently conjugated secondary antibodies in a 3:2 primary:secondary molar ratio (Jackson ImmunoResearch). Samples were incubated in EasyIndex (LifeCanvas Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... The antibody binding was visualized by incubation with secondary antibodies conjugated with Cyanine (Cy)-3 (1:400; Jackson ImmunoResearch Laboratories) for 2h ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were permeabilized with 0.3% Triton X-100 in PBS (PBS-T) and blocked with 3% normal donkey serum (Jackson ImmunoResearch) in PBS-T (PBS-T DS ...
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bioRxiv - Neuroscience 2023Quote: ... recorded slices were blocked in PBS containing 0.3% Triton (PBS-T 0.3%) and 3% normal donkey serum (NDS; Jackson ImmunoResearch, USA) at room temperature for 60 min before incubation in PBS-T 0.3% with 3% NDS and CF405-conjugated streptavidin (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were washed in PBS with 0.1% Triton X-100 (PBST, pH 7.4) and blocked in 3% normal goat/donkey serum (Jackson ImmunoResearch Laboratories) and 2% BSA (Sigma ...
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bioRxiv - Neuroscience 2024Quote: Sections were rinsed 3 x 15 min with PBS and incubated in appropriate secondary antibodies (Jackson ImmunoResearch, West Grove, PA) conjugated to Alexa 488 ...
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bioRxiv - Immunology 2021Quote: ... Bound antibodies were detected with 50 μl of 1 μg/ml alkaline phosphatase-conjugated anti-human IgA and horseradish peroxidase-conjugated anti-human IgG (diluted in PBS containing 0.1% Tween 20 and 1% BSA) antibody (Jackson Immunoresearch, West Grove, PA) for 2 hours at 37°C in 5% CO2 and then developed with VECTOR Blue ...
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bioRxiv - Cancer Biology 2019Quote: ... antibody binding was detected by incubation with peroxidase-conjugated goat anti-human IgG (F(ab’)2 fragment specific) (Jackson ImmunoResearch, West Grove, PA) and TMB solution ...
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bioRxiv - Microbiology 2021Quote: ... to label for RBD expression and 1:200 Alexa Fluor-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064) to label for bound plasma antibodies ...
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bioRxiv - Microbiology 2019Quote: ... anti–human IgG conjugated with HRP (Bio-Rad Laboratories, cat. 172-1050, or Jackson ImmunoResearch, West Grove, PA, USA, cat. 309-035-003) was added and incubated for 2 h at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Plasma binding to cell-surface Spike was revealed using fluorescent secondary antibodies able to detect all Ig isotypes (anti-human IgM+IgG+IgA; Jackson ImmunoResearch Laboratories, Inc.) or specific to IgG isotype (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... coated in tandem with 10 μg/mL MERS-CoV spike and 10 μg/mL OC43-CoV spike were re-suspended in a cocktail of 2.5 μg/mL goat anti-human IgG-Alexa Fluor 647 (Jackson ImmunoResearch, 109-606-170) and goat anti-human IgA-Cy3 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... The plates were incubated for 1 hour at RT followed by washing and addition of 100 μl of anti-human IgA + IgG + IgM (H+L) conjugated to HRP (Jackson ImmunoResearch Laboratories, Inc.) diluted 1:50 000 in blocking buffer ...
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bioRxiv - Immunology 2021Quote: ... Antibody binding to the beads was detected with 2.5 µg/mL goat anti-human IgG-Alexa Fluor 647 (Jackson Immunoresearch 109-606-170), goat anti-human IgA-Cy3 (Jackson Immunoresearch 109-166-011 ...
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bioRxiv - Microbiology 2021Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound monoclonal antibody or 1:200 Alexa-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064 ...
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bioRxiv - Microbiology 2021Quote: ... to label for bound monoclonal antibody or 1:200 Alexa-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064) to label for bound plasma antibodies ...
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bioRxiv - Immunology 2020Quote: ... The wells were washed five times with PBS and probed with horseradish peroxidase (HRP)-conjugated secondary antibody [goat anti-human IgG (Jackson ImmunoResearch; 1:5000)] for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with APC-conjugated polyclonal goat-anti-human IgG F(ab’)2 antibody (Jackson immunoresearch, 1:500). Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech ...
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bioRxiv - Microbiology 2020Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound antibody ...
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bioRxiv - Microbiology 2021Quote: ... samples were diluted at 1:200 and donkey F(ab’)2 anti-human IgM Fc5μ at 1:400 dilution (1 mg/ml, Jackson ImmunoResearch Laboratories, Inc.) was used as the secondary antibody for detection.
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bioRxiv - Immunology 2020Quote: ... bound IgG molecules were revealed with peroxidase conjugated goat anti-human IgG antibodies (Jackson ImmunoResearch, 1/10 000 in 1% chicken egg albumin) and TMB reagent ...
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bioRxiv - Immunology 2021Quote: ... and HRP-conjugated goat polyclonal anti-human IgG F(ab’)2 fragment antibodies (Jackson ImmunoResearch 109-036-008, 1:5000 dilution in PBSM) were used as secondary detection antibodies for sera and Motavizumab ...
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bioRxiv - Immunology 2021Quote: ... Bound IgG was detected using 1μg/mL red phycoerythrin (R-PE)-conjugated goat anti-human IgG antibodies (Jackson ImmunoResearch, Inc., West Grove, PA) and resuspended in MAGPIX® drive fluid before being analyzed on a MAGPIX® Instrument (Luminex Corporation ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 1 hr at RT.Detection was measured with alkaline phosphatase-conjugated goat anti-human IgG Fcγ (Jackson ImmunoResearch 109-005- 008) at 1:2000 dilution in 1% BSA in PBS for 1hr ...
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bioRxiv - Microbiology 2022Quote: ... to label for RBD expression and 1:200 Alexa Fluor-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064) to label for bound plasma antibodies ...
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bioRxiv - Microbiology 2022Quote: ... to label for RBD expression and 1:200 Alexa Fluor-647-conjugated goat anti-human-IgG Fc-gamma (Jackson ImmunoResearch 109-135-098) to label for bound NHP antibodies or Alexa Fluor-647-conjugated goat anti-mouse-IgG Fc-gamma (Jackson ImmunoResearch 115-605-008 ...
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bioRxiv - Immunology 2023Quote: ... Bound autoreactive antibodies were detected with 1µg/ml AlexaFluor647-conjugated F(ab’)2 goat anti-human IgG-Fc (Jackson ImmunoResearch, 109-606-098). Secondary staining was performed in same manner as primary staining ...
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bioRxiv - Bioengineering 2023Quote: ... The plates were washed four times and then incubated with Peroxidase AffiniPure Goat Anti-Human IgG (H+L) (Jackson ImmunoResearch, 1:10,000 dilution) at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... clone CMYC-45F) to label for RBD expression and 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch, cat. 109-115-098) to label for bound mAb.
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:300 dilution of goat anti-human Fc Alexa Fluor 647 (Jackson ImmunoResearch 109-605-098) in PBSB on ice for 4 min ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:300 dilution of goat anti-human Fc Alexa Fluor 647 (Jackson ImmunoResearch, 109-605-098) in PBSB on ice for 4 min ...
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bioRxiv - Immunology 2023Quote: ... The CD40 binding signal was detected by adding a 1:5,000 dilution of goat anti-human Fab/F(ab’)2 IgG secondary-HRP antibody (Jackson ImmunoResearch, 109-035-006) or a 1:20,000 dilution of goat anti-human Fc IgG secondary-HRP antibody (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... 1:75,000) against human kisspeptin-54 (aa 68-121 of NP_002247.3) (9, 53) using biotinylated secondary antibodies (Jackson ImmunoResearch Europe, Cambridgeshire, UK; 1:500), the ABC Elite reagent (Vector ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in a blocking solution composed of PBST with 3% normal donkey serum (Jackson ImmunoResearch, #017-000-121) for 15 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... The slides were washed for 15 min with 1x PBS/0.1% Tween20 post primary antibody treatment followed by 1 hour incubation at room temperature with 1x PBS+3%BSA containing secondary antibodies anti rabbit IgG-Alexa 488 or anti rat IgG-Alexa 594 (all from Jackson ImmunoResearch). The nuclear stain DAPI (2μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... tissue was incubated for 3 hrs at room temp with biotinylated donkey anti-rabbit (1:500, Jackson Immunoresearch 711-065-152), then washed ...
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bioRxiv - Molecular Biology 2020Quote: ... sections were rinsed three times in 1X PBS and incubated in secondary antibody solution Cy-3 donkey anti-rabbit (1:500; Jackson ImmunoResearch) or Cy-3 donkey anti-mouse (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... neurons were incubated for 3-h at room RT in biotin-streptavidin conjugated AffiniPure goat anti-rabbit IgG (1:200; Jackson Immunoresearch), washed three times with 0.1 M cacodylate and then incubated for 3-h at RT in streptavidin HRP conjugate (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Selected slices were then blocked for 3 h at room temperature in a PBS solution containing 5% normal donkey serum (NDS; Jackson ImmunoResearch) and 1% of non-ionic detergent (Triton® X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed with 0.01M PBS (10 Min × 3 times) and incubated in FITC or texas red-conjugated secondary antibody (1:200, Jackson ImmunoResearch Laboratories) in dilution buffer (1% BSA and 0.3% Triton X-100 in 0.01M PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... brain slices were incubated with the following secondary antibodies in the blocking solution at RT 3 hours: donkey anti-mouse-AlexaFluor 488 (715-545-150; 1:300; Jackson ImmunoResearch), donkey anti-G.P.-RRX (706-295-148 ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies against whole Ig or Ig light-chain conjugated to horseradish peroxydase or Cyanine-2 and −3 were purchased from Jackson ImmunoResearch. Phalloidin-Fluoprobes647 (FP-BA0320 ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were incubated for 3 h at room temperature with 1:250 anti-mouse DyLight 650 (Jackson ImmunoResearch, Burlington, ON, Canada) secondary antibody diluted in 0.1 M PB containing 1.5% heat inactivated horse serum ...
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bioRxiv - Cell Biology 2022Quote: ... 2016)) and secondary antibodies were anti-rat coupled to Alexa Fluor 488 and anti-rabbit coupled to Cyanine 3 (Jackson ImmunoResearch) both diluted to 1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were then incubated for 60 min at 37°C with the respective secondary antibodies diluted in 3% BSA: AMCA-conjugated anti-chicken (1:200; Jackson ImmunoResearch), Alexa Fluor® 488 conjugated anti-rabbit (1:500 ...