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Citations for Jackson ImmunoResearch :
551 - 600 of 822 citations for Diethyl 2 chlorothiazol 5 yl methylphosphonate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Fc receptors were blocked by incubating cells in PBS supplemented with 5% FCS with 250 µ g.ml−1 purified mouse IgG (Jackson ImmunoResearch Laboratories). Antibody stainings were performed in PBS/5% FCS on ice for 30 minutes with optimal dilutions of commercially-prepared antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3 times for 5 minutes each with PBT (0.5% Triton X-100 in PBS) and incubated with the corresponding secondary antibodies Alexa Fluor 488 (Anti-Chicken IgG, Jackson ImmunoResearch Labs) and Cy3 (Anti-Rabbit IgG ...
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bioRxiv - Immunology 2020Quote: ... CAR detection was performed using anti-Fab specific antibody at day 5 post-transduction (109-606-006, Jackson ImmunoResearch, West Grove, PA).
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bioRxiv - Immunology 2021Quote: ... Plated peptides were incubated with the individual monoclonal antibodies (5 µg/ml diluted in blocking buffer) and further incubated with donkey anti-human alkaline phosphatase-conjugated secondary antibody (Jackson ImmunoResearch, USA). Immune complexes were identified following incubation with SIGMAFAST™ PNPP (SIGMA ...
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bioRxiv - Cell Biology 2021Quote: ... membrane was incubated with secondary Goat anti-Chicken HRP (1:5000 in 5%BSA/1X PBS-T, Jackson ImmunoResearch, 103-035-155) then incubated for 2 min in the dark with ECL Western Blotting Substrate (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... it was incubated for 30 min in PBS with 5% non-fat milk and then for 1h at RT with horseradish peroxidase-conjugated secondary anti-mouse antibody (1:10000 dilution in PBS with 5% non-fat milk, Jackson Immunoresearch Laboratories), and washed again ...
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bioRxiv - Physiology 2023Quote: ... slices were washed 3×5 min in PBS 0.01M at RT – 4 mL/well and incubated in donkey anti-guinea pig-AF647 (Jackson ImmunoResearch, 1:500) diluted in [PBS 0.01M + 0.3% Triton] ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were subsequently washed four times for 5 minutes in TBS-T before secondary antibody (Jackson ImmunoResearch, 711-035-152, 1:15,000) was added for 1 hour at room temperature in 5% skim milk ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were permeabilized and blocked for 1–2 h at room temperature (RT) in 1X PBS with 0.5% Triton-X (0.5% PBST) and 5–10% normal donkey serum (NDS, Jackson ImmunoResearch 017-000-121). Primary and secondary antibodies were diluted in 0.5% PBST and 5% NDS ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 3X using TBST-High Salt for 5 minutes on a rocking surface at room temperature before being incubated with secondary antibodies conjugated with Horseradish Peroxidase (Jackson ImmunoResearch Labs) for 45 minutes in 5% Non-fat dry milk rocking at room temperature ...
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bioRxiv - Immunology 2023Quote: ... before cultured in complete RPMI-1640 media without/with 5 μg/mL goat anti-mouse IgM (Jackson ImmunoResearch; cat # 115-006-075) and/or recombinant murine 25 ng/ml IL-4 (Peprotech ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were permeabilized and blocked in a single step with 0.2% Triton-X and 5% donkey serum (Jackson Immunoresearch, 017-000-121). Primary antibodies were subsequently added and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Slide regions were washed in PBS for 5 mins and then blocked with 10% normal goat (Jackson ImmunoResearch, West Grove, PA, USA) or donkey serum (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... washed in PBST 3 times (5 min in each time) and incubated with secondary antibodies (mouse anti-Cy3, Jackson Immunoresearch,1:300) for 1h ...
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bioRxiv - Developmental Biology 2024Quote: Samples were washed twice for 10 minutes with PBS-T (0.5% Triton-X100 in PBS) and incubated with Cy3 and/or Alexa Fluor 647-conjugated secondary antibody (1:500, Jackson ImmunoResearch Laboratories), DAPI (4’,6-Diamidino-2-Phenylindole ...
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bioRxiv - Immunology 2021Quote: ... Cells were then blocked for 1 hour in PFN buffer (PBS without Ca2+ and Mg2+ and containing 10% FBS and 0.02% azide) with 2% normal goat serum (Jackson ImmunoResearch). Cells were incubated for 1 hour with the appropriate primary antibodies diluted in blocking medium ...
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bioRxiv - Bioengineering 2021Quote: ... we labeled transduced T cells with Alexa Fluor 647- labeled rabbit anti-mouse F(ab)2 antibody from Jackson ImmunoResearch Laboratories Inc ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated with anti-rabbit-Cy2 and anti-rat-Cy3 secondary antibodies for 2 h (1:400, Jackson Immunoresearch, #711-225-152 ...
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bioRxiv - Neuroscience 2020Quote: ... The primary antibody against RFP was detected with a 2-hour incubation in diluted donkey-anti-rabbit-IgG-Cy3 antibody in blocking buffer (1:1,000, Jackson ImmunoResearch). Finally ...
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bioRxiv - Microbiology 2021Quote: ... and for multiplex beadbased assay with anti-mouse IgG (Fc-specific) F(ab’)2 PE (Jackson ImmunoResearch, West Grove PA). Data were acquired on a Magpix instrument (Luminex ...
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bioRxiv - Immunology 2020Quote: ... then detected by Alexa Fluor 647 goat anti-human secondary antibody (2 μg/ml; 109-605-098, Jackson ImmunoResearch Laboratories). In tests of their specificity for binding RBC ...
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bioRxiv - Neuroscience 2020Quote: ... After incubation (2 h) with appropriate fluorescent secondary antibodies (Cy3-or Cy2-coupled fluorescent secondary antibodies, 1:200; Jackson ImmunoResearch Laboratories catalog #715-165-150 and #715-545-150 respectively) ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were incubated for 2 hours with a species-specific secondary antibody conjugated with green Alexa fluorophore (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Developmental Biology 2021Quote: ... After 3x washing in PBT (with 0.1% Tween 20) embryos were incubated for 2 hours at room temperature with secondary antibodies from Jackson Immunoresearch. Vectashield with DAPI or TSO was used as mounting medium.
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bioRxiv - Cell Biology 2023Quote: ... AffiniPure F(ab’)2-goat anti-rat IgG (H+L) Alexa 488 (Jackson ImmunoResearch Labs Cat# 112-546-003, RRID:AB_2338364), F(ab’)2-goat anti-mouse IgG CF568 (Biotium Cat# 20109 ...
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bioRxiv - Bioengineering 2023Quote: ... and a 1000x dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson Immunoresearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Cell Biology 2023Quote: ... or allophycocyanin-conjugated (APC) AffiniPure F (ab’)2 fragment goat anti-Rat IgM (1:100, Jackson ImmunoResearch, 112-136-075) at 4°C for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... and 36 µg rabbit anti-HA-tag (Cell Signalling Technology #3724) followed by fluorescently conjugated secondary antibodies in a 3:2 primary:secondary molar ratio (Jackson ImmunoResearch). Samples were incubated in EasyIndex (LifeCanvas Technologies ...
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bioRxiv - Immunology 2023Quote: ... the TCR was stimulated by addition of 15 μg/ml of the CD3 antibody clone UCHT1 or alternatively by restimulation with CD3 mAbs (TR66 clone) and crosslinking by 1.3 μg/mL anti-mouse IgGF(ab)’2 fragments (Jackson ImmunoResearch). The ratio of Ca2+-bound Indo-1 versus Ca2+-free Indo-1 was analyzed using FlowJo 9 software (Beckman Coulter).
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bioRxiv - Cell Biology 2022Quote: ... MilliporeSigma) was applied for 2 h at RT followed by secondary antibody (fluorophore-conjugated IgG, 1:500; Jackson ImmunoResearch Laboratories) for 1 h at RT ...
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bioRxiv - Biophysics 2023Quote: ... permeabilization and and blocking were performed with PBS (with 0.4% (v/v) Triton X-100%) and 2% immunoglobulin G-free BSA (Jackson ImmunoResearch) for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: ... and 1:1000 dilution of goat anti-human Fc F(‘ab)2 Alexa Fluor 488 (Jackson ImmunoResearch, 109-546-170) on ice for 4 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 mM EDTA) and incubated overnight at 4°C with rotation in the respective antibody (IgG [1:100; Jackson ImmunoResearch, 111-005-003 ...
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bioRxiv - Microbiology 2023Quote: ... plates were coated with either 2 µg/ml of donkey anti-mouse IgG (Affinipure; Jackson ImmunoResearch Laboratories, West Grove, PA), carbonate buffer (0.0875 M Na2CO3 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed and incubated for 2 h with FITC-conjugated donkey anti-rabbit antibody (1:150; RRID:AB_2315776, Jackson ImmunoResearch, US) in TBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was again rinsed three times in 0.1M NaPb for 10 min followed by 2-h in biotin-SP-conjugated donkey anti-rabbit (1:300; Jackson ImmunoResearch) secondary antibody and AlexaFluor-488 donkey anti-chicken secondary antibody (1:300 ...
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bioRxiv - Microbiology 2024Quote: ... goat anti-mouse conjugated with HRP (1:5,000, 2 hours at room temperature, catalog no. 111-035-146; Jackson ImmunoResearch), goat anti-rat conjugated with HRP (1:5,000 ...
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bioRxiv - Microbiology 2024Quote: ... goat anti-rat conjugated with HRP (1:5,000, 2 hours at room temperature, catalog no. 115-035-143; Jackson ImmunoResearch). Western blots were developed with Thermo Scientific SuperSignal West Pico and Femto PLUS Chemiluminescent Substrate ...
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bioRxiv - Immunology 2024Quote: ... B cells were stimulated with 10 μg/mL polyclonal F(ab’)2 goat anti-mouse IgM (anti-μ) (Jackson ImmunoResearch) or 5 μg/mL anti-IgDa (clone AMS 9.1 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with 2 μg/mL goat anti-human IgG (H+L) Alexa647 (Jackson ImmunoResearch, Cat# 109-605-003) for 30 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... After extensive washes with 2× SSC at 37°C the probe was revealed using Cy-conjugated streptavidin (Jackson Immunoresearch Laboratories), followed by immunostaining as described above.
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bioRxiv - Immunology 2020Quote: ... circumscribed with ImmEdge pen (Vector) and blocked in TBST (100mL Tris-HCl, 150mM NaCl, 0.05% Tween-20) containing 5% Donkey serum (Jackson ImmunoResearch #017-000-121) for 2 hours at RT ...
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bioRxiv - Genetics 2021Quote: ... The fixed tissues were then subjected to three rounds of 15 minute-each wash with 0.1% PBST (0.1% Triton X-100 in PBS) and blocked with 5% donkey serum (Jackson ImmunoResearch Laboratories, 107-000-121) for 1 hour at room temperature followed by incubation with primary antibodies in 5% donkey serum at 4°C ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h at RT with blocking solution of 5% goat serum in PBS (Jackson ImmunoResearch, catalog no. 005–000-121). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (GeneTex ...
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bioRxiv - Plant Biology 2022Quote: ... the seedlings were washed for three times 5 min each and then incubated in the secondary antibody Cy-3-goat anti-mouse IgG (1:200; Jackson Immunoresearch, http://www.jacksonimmuno.com). The imaging was performed on a Nikon laser scanning microscope (Eclipse Ti equipped with Nikon C2 Si laser scanning unit ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were blocked in 5% w/v bovine serum albumin (IgG-free and protease-free BSA, Jackson ImmunoResearch Laboratories, West Grove, PA) in 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed 3 times for 5 minutes each in PBS+0.3% TritonX-100 and incubated with appropriate fluorescent conjugated secondary antibody (Jackson ImmunoResearch, West Grove, PA) for 45 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Antibodies for O1 and O4 were diluted in N2B27 base medium supplemented with 5% Donkey Serum (v/v) (Jackson ImmunoResearch, 017-000-121) and added to wells for 18 minutes at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections of 20 um thickness were blocked in PBS + 0.3% Triton X-100 and 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121) for one hour ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 M glycine, 0.1% Triton-X, syringe filtered) for 10 min, then blocking buffer (1X PBS, 5% goat serum (Jackson ImmunoResearch; 005-000-121), 2% bovine serum albumin (Sigma-Aldrich ...