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Citations for Jackson ImmunoResearch :
501 - 550 of 589 citations for 5 tert Butyl 3 isocyanatoisoxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The nuclei were then quickly washed with assay buffer and resuspended in antibody incubation buffer (assay buffer with 5% NGS (Jackson ImmunoResearch), 0.02% digitonin ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... Samples were rinsed three times with PBS and blocked for 15 min using PBS with 5% goat serum (Jackson ImmunoResearch Laboratories) and 0.5% Triton X-100 (Tx ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed repetitively in TBS and TBS + 0.3% Triton X-100 and incubated with Cy2-conjugated streptavidin (5 µg/ml, Jackson Immunoresearch Lab.) for 2.5 hours at RT ...
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bioRxiv - Neuroscience 2022Quote: ... in 0.3% PBT were followed by 30 min incubation in blocking solution with 5% NGS (005-000-121, Jackson Immunoresearch Laboratories) in PBT ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating sections were washed three times for 10 minutes in 0.1M Tris-buffered saline (TBS) and then incubated in 0.1M TBS with 5% blocking buffer (Normal Donkey Serum, Jackson Immunoresearch, NC9624464) and 0.3% Triton-X 100 for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... and blocked for 1h at room temperature (RT) or overnight at 4 °C in 5% normal donkey serum (NDS) in PBS-TX (Jackson Immunoresearch). Primary antibodies were chicken anti-GFP (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed in PBS/0.05% (three times, 5 min each) and incubated with Alexa Fluor (488, 568 and/or 647)-conjugated secondary antibodies (Jackson ImmunoResearch) diluted in blocking solution (1:200) ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 5% skim milk and incubated with respective primary antibodies followed by HRP-conjugated secondary antibodies (Jackson ImmunoResearch Labs Cat# 111-035-045 ...
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bioRxiv - Biochemistry 2023Quote: ... Triton X-100 in PBS at room temperature for 15 min and washed with the blocking buffer [5% (v/v) normal goat serum (NGS, Jackson ImmunoResearch) and 0.1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Cochleas were permeabilized in 0.2% Triton X-100 in 1x PBS for 10 min and blocked in 5% normal donkey serum (Cat# 017-000-121, Jackson ImmunoResearch) diluted in 1x PBS (blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... 1:5) at 4 °C overnight and with the secondary FITC-conjugated or TRITC-conjugated anti-mouse secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Immunology 2023Quote: ... the sections were incubated in Cy-5 anti-rabbit secondary antibody (1:1000, Jackson ImmunoResearch Labs Inc., AB_2340607, West Grove, USA) for 1 hour at 4°C and counterstained with 4’,6-Diamidino-2-Phenylindole ...
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bioRxiv - Neuroscience 2023Quote: ... tissues were rinsed in PBS and blocked in Tris-Buffered Saline (TBS) supplemented with 5 % (vol/vol) Normal Donkey Serum (Jackson Immunoresearch) and 0.3 % (wg/vol ...
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bioRxiv - Biophysics 2023Quote: ... Blots were washed three times with TBST for 5 min prior to the addition of the secondary antibody (goat anti-rabbit tagged with horseradish peroxidase, Jackson ImmunoResearch, West Grove ...
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bioRxiv - Cell Biology 2023Quote: ... Detection was achieved using secondary antibodies conjugated to horseradish peroxidase (#111-036-003, 106-006-003, 1:5 mL, Jackson ImmunoResearch) and treated with enhanced chemiluminescence (#1705061 ...
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bioRxiv - Immunology 2023Quote: ... Purified human naïve B cells were cultured in 96-well plates in the presence of AffiniPure F(ab’)₂ Fragment goat anti-human IgG + IgM (5 μg/mL, CAT: 109-006-127, Jackson ImmunoResearch), anti-human CD40 (5 μg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... the slices were rinsed 3 times for 5 minutes in PBS and incubated in the relevant secondary antibodies mix (donkey anti-sheep Alexa Fluor 405, Jackson Immunoresearch #713-475-003 ...
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bioRxiv - Immunology 2024Quote: ... diluted in 5% (wt/vol) dried milk in TBS-T and then with Peroxidase-AffiniPure Goat Anti-Rabbit IgG (Jackson ImmunoResearch) at 1 in 5,000 ...
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bioRxiv - Microbiology 2024Quote: ... except that primary antibodies were serially diluted 5-fold and followed by incubation with a 1:3000 dilution of HRP-labeled goat anti-human IgG (Jackson Immunoresearch) in casein for 45 min at 37°C ...
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Foxn3 is part of a transcriptional network that regulates cilia genes in the developing mouse retinabioRxiv - Developmental Biology 2024Quote: Retina sections were treated with a blocking solution (1% unlabeled adinity-purified Fab fragment donkey anti-mouse IgG (H+L) and 5% donkey serum (Jackson Immunoresearch) in PBS containing 0.1% Triton X-100 (ThermoFisher) ...
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bioRxiv - Biochemistry 2020Quote: ... The α-actin secondary antibody was a Cy-3 conjugated AffiniPure donkey anti-mouse at a dilution of 1:500 (Jackson ImmunoResearch Laboratories Inc. #715-166-150). Bands for p-ERK ...
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bioRxiv - Neuroscience 2020Quote: ... selected coronal sections were rinsed three times in TBS and incubated in blocking solution (3% normal donkey serum, Jackson Immunoresearch AB2337258; 0.3% Triton X-100 in TBS) at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated for 45 min with the secondary antibody Cy™3 AffiniPure F(ab’)2 Fragment Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., 1:200 dilution). DAPI (0.2 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were incubated with primary antibodies (see Key Resources Table) overnight and subsequent incubated with secondary antibodies labeled with Alexa Fluor 488- (Jackson ImmunoResearch, 115-545-003, 3 μg/mL) or Cy3- (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2024Quote: Cav2sfGFP (Gratz et al. 2019) localization (Figs. 2 and 3): Primary antibodies (α-brp nc-82, 1:400, DSHB; α-HRP rabbit, 1:500, Jackson Immunoresearch Cat# 323-005-021) were applied in PBS-Tx 0.3 % overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3 x 5 mins in 1X TBST and incubated in 5% non-fat dry milk in 1X TBST containing anti-rabbit (H+L) horseradish peroxidase (Jackson ImmunoResearch Laboratories) secondary antibodies at a dilution of 1:20,000 for 1 hour at room temp ...
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bioRxiv - Cell Biology 2020Quote: ... Fc receptors were blocked by incubating cells in PBS supplemented with 5% FCS with 250 µ g.ml−1 purified mouse IgG (Jackson ImmunoResearch Laboratories). Antibody stainings were performed in PBS/5% FCS on ice for 30 minutes with optimal dilutions of commercially-prepared antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3 times for 5 minutes each with PBT (0.5% Triton X-100 in PBS) and incubated with the corresponding secondary antibodies Alexa Fluor 488 (Anti-Chicken IgG, Jackson ImmunoResearch Labs) and Cy3 (Anti-Rabbit IgG ...
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bioRxiv - Immunology 2020Quote: ... CAR detection was performed using anti-Fab specific antibody at day 5 post-transduction (109-606-006, Jackson ImmunoResearch, West Grove, PA).
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bioRxiv - Immunology 2021Quote: ... Plated peptides were incubated with the individual monoclonal antibodies (5 µg/ml diluted in blocking buffer) and further incubated with donkey anti-human alkaline phosphatase-conjugated secondary antibody (Jackson ImmunoResearch, USA). Immune complexes were identified following incubation with SIGMAFAST™ PNPP (SIGMA ...
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bioRxiv - Cell Biology 2021Quote: ... membrane was incubated with secondary Goat anti-Chicken HRP (1:5000 in 5%BSA/1X PBS-T, Jackson ImmunoResearch, 103-035-155) then incubated for 2 min in the dark with ECL Western Blotting Substrate (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... it was incubated for 30 min in PBS with 5% non-fat milk and then for 1h at RT with horseradish peroxidase-conjugated secondary anti-mouse antibody (1:10000 dilution in PBS with 5% non-fat milk, Jackson Immunoresearch Laboratories), and washed again ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were subsequently washed four times for 5 minutes in TBS-T before secondary antibody (Jackson ImmunoResearch, 711-035-152, 1:15,000) was added for 1 hour at room temperature in 5% skim milk ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were permeabilized and blocked for 1–2 h at room temperature (RT) in 1X PBS with 0.5% Triton-X (0.5% PBST) and 5–10% normal donkey serum (NDS, Jackson ImmunoResearch 017-000-121). Primary and secondary antibodies were diluted in 0.5% PBST and 5% NDS ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 3X using TBST-High Salt for 5 minutes on a rocking surface at room temperature before being incubated with secondary antibodies conjugated with Horseradish Peroxidase (Jackson ImmunoResearch Labs) for 45 minutes in 5% Non-fat dry milk rocking at room temperature ...
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bioRxiv - Physiology 2023Quote: ... slices were washed 3×5 min in PBS 0.01M at RT – 4 mL/well and incubated in donkey anti-guinea pig-AF647 (Jackson ImmunoResearch, 1:500) diluted in [PBS 0.01M + 0.3% Triton] ...
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bioRxiv - Immunology 2023Quote: ... before cultured in complete RPMI-1640 media without/with 5 μg/mL goat anti-mouse IgM (Jackson ImmunoResearch; cat # 115-006-075) and/or recombinant murine 25 ng/ml IL-4 (Peprotech ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were permeabilized and blocked in a single step with 0.2% Triton-X and 5% donkey serum (Jackson Immunoresearch, 017-000-121). Primary antibodies were subsequently added and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Slide regions were washed in PBS for 5 mins and then blocked with 10% normal goat (Jackson ImmunoResearch, West Grove, PA, USA) or donkey serum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: Samples were washed twice for 10 minutes with PBS-T (0.5% Triton-X100 in PBS) and incubated with Cy3 and/or Alexa Fluor 647-conjugated secondary antibody (1:500, Jackson ImmunoResearch Laboratories), DAPI (4’,6-Diamidino-2-Phenylindole ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for 3 hours at 22-25 °C with Cy3-conjugated anti-mouse and Alexa488-conjugated anti-chicken secondary antibodies (Jackson ImmunoResearch, 715-165-150 and 711-225-152) at 1:500 and 1:1000 dilution in a solution of 1.5% BSA in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were washed 3 times with PBS and then incubated with 1:200 Alexa Fluor 488 donkey anti-mouse IgG (H+L) (Jackson ImmunoResearch, West Grove, PA, USA; 715-545-151), 1:200 Fluorescein (Cy3 ...
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bioRxiv - Immunology 2020Quote: ... circumscribed with ImmEdge pen (Vector) and blocked in TBST (100mL Tris-HCl, 150mM NaCl, 0.05% Tween-20) containing 5% Donkey serum (Jackson ImmunoResearch #017-000-121) for 2 hours at RT ...
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bioRxiv - Genetics 2021Quote: ... The fixed tissues were then subjected to three rounds of 15 minute-each wash with 0.1% PBST (0.1% Triton X-100 in PBS) and blocked with 5% donkey serum (Jackson ImmunoResearch Laboratories, 107-000-121) for 1 hour at room temperature followed by incubation with primary antibodies in 5% donkey serum at 4°C ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h at RT with blocking solution of 5% goat serum in PBS (Jackson ImmunoResearch, catalog no. 005–000-121). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (GeneTex ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were blocked in 5% w/v bovine serum albumin (IgG-free and protease-free BSA, Jackson ImmunoResearch Laboratories, West Grove, PA) in 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed 3 times for 5 minutes each in PBS+0.3% TritonX-100 and incubated with appropriate fluorescent conjugated secondary antibody (Jackson ImmunoResearch, West Grove, PA) for 45 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Antibodies for O1 and O4 were diluted in N2B27 base medium supplemented with 5% Donkey Serum (v/v) (Jackson ImmunoResearch, 017-000-121) and added to wells for 18 minutes at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections of 20 um thickness were blocked in PBS + 0.3% Triton X-100 and 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121) for one hour ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 M glycine, 0.1% Triton-X, syringe filtered) for 10 min, then blocking buffer (1X PBS, 5% goat serum (Jackson ImmunoResearch; 005-000-121), 2% bovine serum albumin (Sigma-Aldrich ...