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Citations for Jackson ImmunoResearch :
5351 - 5400 of 5568 citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Next day, the cells on the slides were incubated with secondary antibodies (donkey anti-rabbit, 1:500) (Jackson ImmunoResearch, Cat. No. 711-605-152) conjugated with Cy5 for 2 h at room temperature ...
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bioRxiv - Genomics 2024Quote: ... coverslips were washed and RL1 antibody was immunostained with goat-anti-mouse-Alexa-Fluor-488 secondary antibody (Jackson ImmunoResearch Laboratories, cat#115-545-003) diluted 1:500 in PBS/NGS ...
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bioRxiv - Immunology 2024Quote: ... 1 ng/ml mouse IL-6 and 10 µg/ml FITC-F(ab’)2 Goat anti-mouse IgG (Jackson Immunoresearch, USA, # 115-096-071). The cell mixture was plated in Omnitray dish (Nunc ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated for 15 minutes on ice with 100 mL of 1:500 APC-F(ab’)2 fragment goat anti-human IgG (Jackson Immunoresearch #109-136-098) diluted in FACS buffer ...
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bioRxiv - Neuroscience 2024Quote: ... washed 3 times in PB 0.1 M, a secondary antibody solution (PB 0.1M, Triton 0.3%, NGS 2%, goat anti-rabbit Cyanine Cy3TM (Jackson Immunoresearch, 111-165-003, RRID: AB_2338000), goat anti-mouse Cy5TM (Jackson Immunoresearch ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 4x in PBT and incubated for 90 min in secondary antibody (Alexafluor 488 AffiniPure Donkey Anti-Rabbit, 1:1000, Jackson Immunoresearch # 711-545-152). The secondary antibody was then washed off with PBT in 3X10 min washes ...
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bioRxiv - Neuroscience 2024Quote: ... blocked tissues overnight with blocking buffer and stained with a polyclonal goat anti-mouse F(ab’)2 fragment (1:100; Jackson ImmunoResearch 115-005-072) and rabbit anti-mouse NF200 (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated in secondary antibody solution (0.1% Triton 100X in PBS) for 2 hours at room temperature with the following secondary antibodies: Cy3 donkey anti-rabbit IgG (1:1000, Jackson ImmunoResearch, #711-165-152) and Cy5 donkey anti-guinea pig IgG (1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated at 4°C overnight with primary antibody at 1:100 in blocking buffer The following day cells were washed 3 times for 5 minutes with 1X TBS then incubated with Cy5 Donkey Anti-Mouse (Jackson ImmunoResearch 715-175-150) at 1:100 in 1X TBS for 2 hours in the dark at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The following day slides were washed with PBS-T and incubated for 1h at rt with 15 μg/ml fab fragment anti-rabbit alexa 647 (Jackson ImmunoResearch, 711-607-003). Slides were stained overnight with 33 μg/ml anti-IgA alexa488 (Abcam ...
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bioRxiv - Plant Biology 2024Quote: ... Membranes were washed 3 times with TBS-T for 15 min at room temperature and incubated with secondary polyclonal anti-rabbit antisera HRP for one hour at room temperature (Jackson ImmunoResearch, 111-035-003), followed by 3 additional washes with TBS-T and visualized by enhanced chemiluminescence technique.
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bioRxiv - Cell Biology 2024Quote: ... rat 70 kDa isoform) (custom-made, as described [66], and mTOR P-S1261 (custom-made, as described [66]. Donkey anti-rabbit-HRP (Jackson ImmunoResearch, 711-095-152) detected ECL-based western blot signals ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then extensively washed with PBS and incubated for 45 minutes at 37°C in a humidified chamber with the secondary anti-rat IgG Alexa 594 antibody (1:500; Jackson ImmunoResearch, #112-585-167) diluted in 3% BSA in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then incubated in the same fresh medium containing biotinylated goat anti-rabbit IgG antibody (1:1,000; Jackson ImmunoResearch, West Grove, PA, USA) for 2 h at room temperature ...
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Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activitybioRxiv - Bioengineering 2024Quote: ... yeast were washed in PBS/0.2% BSA and rotated at 18 rpm in 300 μL of same buffer containing 2 μg/mL Alexa488-conjugated goat anti-chicken IgG (Jackson ImmunoResearch, West Grove, PA) for one hour at 4°C ...
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bioRxiv - Immunology 2024Quote: ... After washing twice with 200 μl FACS buffer the cells were incubated in 30 μl of FACS buffer for 20 min with 1:1000 diluted APC-conjugated F(ab’)₂ fragment goat anti-human IgG (Jackson ImmunoResearch, West Grove, USA). Following two further washes with FACS buffer the cells were resuspended in 100 μl FACS buffer with 0.1 µg/ml propidium iodide (BD Pharmingen) ...
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bioRxiv - Immunology 2024Quote: ... goat anti-mouse secondary detection antibody conjugated to HRP was diluted 1:10,000 for IgG (peroxidase conjugated goat anti-mouse IgG H&L, Jackson ImmunoResearch, cat. 115-035-003) and 1:5,000 for IgA (peroxidase conjugated goat anti-mouse IgA ...
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bioRxiv - Immunology 2024Quote: Antibody binding was detected using an Alexa Fluor® conjugated goat anti-human IgG Fc fragment-specific secondary antibody (Jackson ImmunoResearch, West Grove, PA), diluted 1:200 in blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The brain sections were rinsed for three times with PBS and incubated with the secondary antibody AlexaFluor 488 donkey anti-rabbit IgG (1:500, cat#: 711-545-152, Jackson ImmunoResearch Labs, RRID: AB_2313584) and Cy5-conjugated streptavidin (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Brains were washed 4X in PBT at RT and incubated in PBANG containing goat anti-rabbit Cy3 (1:200; Jackson ImmunoResearch, 111-165-003) for 2 nights at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used at 1:10,000 dilution and incubated for 1 hour at room temperature in 5% milk w/ TBST: HRP donkey anti-rabbit (Jackson ImmunoResearch #711-035-152) and HRP goat anti-mouse IgG subclass 1-specific (Jackson ImmunoResearch #115-035-205) ...
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bioRxiv - Biophysics 2021Quote: ... Secondary antibody: Alexa Fluor® 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., code. 715-546-151).
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bioRxiv - Cell Biology 2020Quote: ... cells immunostained for E-cadherin with Alexa Fluor 488 secondary antibody were blocked with 26 µg/ml of AffiniPure Fab Fragment Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, Inc, West Grove, PA) in 5% NGS in PBS overnight before being stained for LAMP1 with Alexa Fluor 594-conjugated goat anti-mouse secondary antibody (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were washed with PBS and immunostained in PBG with secondary antibodies conjugated to fluorescein isothiocyanate (FITC) (donkey anti-mouse IgG; Jackson ImmunoResearch Lab, Inc., 1:125) or Cy3 (donkey anti-rabbit ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed 3X for 10 min each in T-Buffer followed by one wash in TBS and incubation in Alexa Fluor 647-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch, diluted at 1:250) and Alexa Fluor 488-conjugated donkey anti-rat IgG (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... slides were washed and incubated for 1 h at room temperature with anti-rabbit horseradish peroxidase-conjugated antibodies (Jackson ImmunoResearch #111-035-003, 1:500). Peroxidase activity was detected using 3,3’ Diaminobenzidine substrate (BD Pharmingen #550880) ...
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bioRxiv - Physiology 2020Quote: ... The secondary antibodies were peroxidase-conjugated AnffiniPure Goat Anti-Rabbit or mouse IgG H&L (Alexa Fluor® 488; Jackson ImmunoResearch Laboratories, West Grove, PA) at 1:500 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed three times with PBS (pH 7.4) and then incubated for 2 h at room temperature with Alexa 488 goat anti mouse 1:1000 (Jackson ImmunoResearch, cat n° 115-545-166), Alexa 594 goat anti rabbit 1.1000 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... for 30 minutes at 4°C and subsequently labeled with Rhodamine conjugated anti-mouse IgG (Jackson ImmunoResearch, Cat#115-026-062, 1:100 in PBS). Fluorescently labeled nuclei were counted using a Nikon Eclipse TE2000-U at 20x magnification.
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bioRxiv - Molecular Biology 2022Quote: ... Primary antibody was washed in PBS with 0.1% Tween 20 (PBT) before applying a horseradish peroxidase (HRP) conjugated anti-guinea pig antibody (Jackson ImmunoResearch Laboratories, INC., West Grove, PA) at a final concentration of 1:250 for 2 hrs at RT or ON at at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... After three washes with PBS the sample was incubated for 3-4h with a secondary donkey anti-rabbit antibody (711-005-152; Jackson ImmunoResearch, West Grove, PA, USA) that was conjugated to Alexa Fluor 647-NHS at an average degree of labeling of 1.5 ...
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bioRxiv - Neuroscience 2020Quote: ... and subsequently with the fluorophore-conjugated secondary antibodies (Figure 1B5): Alexa Fluor® 488 AffiniPure Donkey Anti-Chicken 1:000 (Jackson ImmunoResearch, 703-545-155), DyLight™ 405 AffiniPure Donkey Anti-Guinea pig IgG (H+L ...
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bioRxiv - Microbiology 2021Quote: ... Slides were washed three times with TBS buffer with 0,05 % Tween-20 and further incubated with anti-rabbit Alexa 647 (Jackson ImmunoResearch Cat. No. 111-606-144), anti-human IgG-Alexa647 (Southern Biotech ...
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bioRxiv - Microbiology 2021Quote: ... overnight at 4°C and then incubated with anti-mouse or rabbit IgG-HRP conjugate (209-035-082 or 309-035-003, Jackson ImmunoResearch, West Grove, PA, USA) for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... above tissue sections under ISH were incubated with chicken anti-NeuN antibody (1:200; Aves, Davis, CA) overnight at 4°C followed by donkey anti-chicken IgG Alexa 488 (Jackson ImmunoResearch labs, West Grove, PA) for 1 hr at room temperature ...
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bioRxiv - Immunology 2020Quote: ... BCR-mediated Ca2+ mobilization was measured for 60s after the cells were stimulated either with 10 μg/mL F(ab′)2 of polyclonal goat anti-mouse IgM (Jackson ImmunoResearch, West Grove, PA, USA) or 0.5mM phosphatidylcholine (PtC ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed in PBS and incubated for 90 min at room temperature in Alexa Fluor 488- or 594-conjugated donkey anti-mouse IgG (1:250; Jackson ImmunoResearch; RRID: AB_2313584; RRID: AB_2340621) in PBS with 0.5% Triton X-100 and 2% normal donkey serum ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were stained with Th antibody (Santa Cruz Biotechnology, #sc-25269; 1/20 dilution; anti-mouse IgG2a secondary Fab fragment conjugated with Alexa 594 (Jackson ImmunoResearch, #115-587-186)) and nuclear staining (SYTOX-G) ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were stained with β-Amyloid (6E10) antibody (Biolegend, #93049; 1/100 dilution; anti-mouse IgG1 secondary Fab fragment conjugated with Alexa 594 (Jackson ImmunoResearch, #115-587-185)) and nuclear staining (SYTOX-G).
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bioRxiv - Bioengineering 2021Quote: ... strips were washed 10 times with PBST and the binding of VHH-Fcs to denatured S was probed by incubating strips with 1 mL of 100 ng/mL anti-human Ig Fc antibody-peroxidase conjugate (Jackson ImmunoResearch, Cat#016-030-084) at room temperature for 1 h ...
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bioRxiv - Bioengineering 2021Quote: ... wells were incubated with 100 µL 1/2500 dilution (320 ng/mL) of goat anti- rabbit:HRP (Jackson ImmunoResearch, West Grove, PA, Cat#111-035-144) in PBSCT for 1 h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... washed twice with PBSB by centrifugation for 5 min at 1200 rpm and then incubated for an additional hour with 50 µL of R-Phycoerythrin AffiniPure F(ab’)₂ Fragment Goat Anti-Human IgG (Jackson ImmunoResearch, Cat#109-116-170) at 250 ng/mL diluted in PBSB ...
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bioRxiv - Bioengineering 2021Quote: ... Approximately 200 RUs of VHH-Fcs (2 µg/mL) were captured on goat anti-human IgG surfaces (4000 RUs, Jackson ImmunoResearch, Cat#109-005-098) at a flow rate of 10 µL/min for 30 s ...
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bioRxiv - Neuroscience 2022Quote: ... tissues were incubated for 2 hours at room temperature in Alexafluor-488 conjugated donkey anti-chicken (1:500; Jackson ImmunoResearch 703-546-155, lot# 142461) in 1% goat serum and 0.3% Triton X100 in PBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then pelleted washed twice with PBSA and subsequently labeled with PE-conjugated goat anti-human IgG (1:100, Jackson ImmunoResearch Labs #109-115-098) and FITC-conjugated chicken anti-cMmyc (1:100 ...
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bioRxiv - Bioengineering 2021Quote: ... The channels were incubated at 25 °C for 60 min with the corresponding secondary antibodies AlexaFluor 594 donkey anti-rabbit IgG (1:1000; Jackson ImmunoResearch, West Grove, PA, USA) and Alexa Fluor 648 donkey anti-rat IgG (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed in PBS and incubated at 4°C in PBS containing 2% donkey serum and secondary antibodies conjugated to anti-rabbit Cy3 (Jackson ImmunoResearch Inc., West Grove, PA) and anti-guinea pig CF405M (Biotium ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then washed 3x with PBS and incubated with the following secondary antibodies in PBS: anti-goat Alexa Fluor® 488 (1:500, Jackson ImmunoResearch, #705-546-147), anti-rabbit Alexa Fluor® 555 (1:500 ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of red-phycoerythrin (R-PE) conjugated F(ab’)2 fragment goat anti-mouse IgG specific to the Fc fragment (Jackson ImmunoResearch, Inc., West Grove, PA) were added at 1 μg/ml to the wells and incubated for 45 minutes ...
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bioRxiv - Immunology 2021Quote: ... live cells expressing S delta 19aa were suspended in QSol Buffer (IntelliCyte Corporation) to which a cocktail of Fc-specific secondary antibodies was added: AF647 goat anti-human IgG (Jackson ImmunoResearch, Cat # 109-605-008), AF488 goat anti-human IgA (Jackson ImmunoResearch ...