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• Transcriptome profiling of tendon fibroblasts at the onset of embryonic muscle contraction reveals novel force-responsive genes

  Pavan Nayak, Arul Subramanian, Thomas Schilling, bioRxiv - Developmental Biology 2022

  Quote: ... Alexa Fluor 647 conjugated donkey anti-mouse IgG (Jackson Immunoresearch, 1:1000 dilution, 715-606-151).
• A CRISPR-Cas9-mediated F0 screen to identify pro-regenerative genes in the zebrafish retinal pigment epithelium

  Fangfang Lu, Lyndsay L. Leach, Jeffrey M. Gross, bioRxiv - Developmental Biology 2022

  Quote: ... and goat anti-rat Cy3 (1:250, Jackson ImmunoResearch Laboratories, 112-165-003). All fluorescent images were captured using an Olympus Fluoview FV1200 laser scanning confocal microscope (Olympus Corporation) ...
• Biphasic release propensity of mucin granules is supervised by TSPAN8

  Wojnacki José, et al., bioRxiv - Cell Biology 2022

  Quote: ... Horseradish root peroxidase-conjugated (Jackson ImmunoResearch) or fluorescently-conjugated (Invitrogen ...
• Ablation of ZC3H11A causes early embryonic lethality and dysregulation of metabolic processes

  Shady Younis, et al., bioRxiv - Cell Biology 2022

  Quote: ... samples were washed by PBS and incubated with the fluorophore conjugated secondary antibodies (Jackson ImmunoResearch) for an hour at room temperature ...
• Transcriptome profiling of tendon fibroblasts at the onset of embryonic muscle contraction reveals novel force-responsive genes

  Pavan Nayak, Arul Subramanian, Thomas Schilling, bioRxiv - Developmental Biology 2022

  Quote: ... Alexa Fluor 488 conjugated donkey anti-chicken IgY (Jackson Immunoresearch, 1:1000 dilution, 703-486-155), Alexa Fluor 647 conjugated donkey anti-mouse IgG (Jackson Immunoresearch ...
• Transcriptome profiling of tendon fibroblasts at the onset of embryonic muscle contraction reveals novel force-responsive genes

  Pavan Nayak, Arul Subramanian, Thomas Schilling, bioRxiv - Developmental Biology 2022

  Quote: ... Secondary antibodies used: Alexa Fluor 594 conjugated donkey anti-rat IgG (Jackson ImmunoResearch – 1:1000 dilution, 712-586-153), Alexa Fluor 488 conjugated donkey anti-chicken IgY (Jackson Immunoresearch ...
• A neuroepithelial wave of BMP signalling drives anteroposterior specification of the tuberal hypothalamus

  Kavitha Chinnaiya, et al., bioRxiv - Developmental Biology 2022

  Quote: ... Secondary antibodies (1:500, Jackson Immunoresearch) were conjugated with anti-Alexa 488 or 594 ...
• FGF signaling regulates salivary gland branching morphogenesis by modulating cell adhesion

  Ayan T. Ray, Philippe Soriano, bioRxiv - Developmental Biology 2022

  Quote: ... All secondary antibodies were used at 1:5,000 dilutions as follows: Alexa Fluor® 488 AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) (Jackson ImmunoResearch Laboratories; #703-545-155); Donkey anti-Rabbit IgG (H+L) ...
• Aquaporin regulates cell rounding through vacuole formation during endothelial-to-hematopoietic transition

  Yuki Sato, et al., bioRxiv - Developmental Biology 2022

  Quote: ... The sections were then washed with PBS and incubated with goat antibody against HRP conjugated to 6-nm gold particles (1:50 dilution, Jackson ImmunoResearch Laboratory) for 1 h at 25ºC ...
• Determining distinct roles of IL-1α through generation of an IL-1α knockout mouse with no defect in IL-1β expression

  R.K. Subbarao Malireddi, et al., bioRxiv - Cell Biology 2022

  Quote: ... Appropriate horseradish peroxidase (HRP)–conjugated secondary antibodies (anti-Armenian hamster [127-035-099], anti-mouse [315-035-047], and anti-rabbit [111-035-047], Jackson ImmunoResearch Laboratories) were used as described previously (Tweedell et al ...
• Ectodysplasin signaling via Xedar is required for mammary gland morphogenesis

  Abigail R. Wark, et al., bioRxiv - Developmental Biology 2022

  Quote: ... Samples were washed in PBST and incubated with Alexa Fluor488(1:250, Jackson ImmunoResearch) and 4′6-diamidino-2-phenylindole (1:5000 ...
• Drosophila Males Use 5′-to-3′ Phased Biogenesis to Make Stellate-silencing piRNAs that Lack Homology to Maternally Deposited piRNA Guides

  Zsolt G. Venkei, et al., bioRxiv - Developmental Biology 2022

  Quote: ... and anti-guinea pig IgG (1:10,000; Jackson ImmunoResearch Laboratories) secondary antibodies were used ...
• A CRISPR-Cas9-mediated F0 screen to identify pro-regenerative genes in the zebrafish retinal pigment epithelium

  Fangfang Lu, Lyndsay L. Leach, Jeffrey M. Gross, bioRxiv - Developmental Biology 2022

  Quote: ... Secondary antibodies used in this study include goat anti-mouse Cy3 (1:250, Jackson ImmunoResearch Laboratories, 115-165-166) and goat anti-rat Cy3 (1:250 ...
• Zinc-based Ultrasensitive Microscopic Barrier Assay (ZnUMBA): a live-imaging method for detecting epithelial barrier breaches with spatiotemporal precision

  Tomohito Higashi, et al., bioRxiv - Cell Biology 2022

  Quote: ... Alexa Fluor 488-conjugated donkey anti-rabbit IgG pAb (#711-545-152) and Alexa Fluor 647-conjugated donkey anti-rat IgG pAb (#712-605-153) were purchased from Jackson ImmunoResearch Laboratories (PA ...
• Improving stem cell-derived pancreatic islets using single-cell multiome-inferred regulomes

  Han Zhu, et al., bioRxiv - Cell Biology 2022

  Quote: ... AlexaFluor® 488-conjugated donkey anti-rabbit IgG (1:1000 dilution, Jackson Immunoresearch 711-545-152); Biotin-conjugated anti-CD26 (1:500 dilution ...
• Endoglin, a novel biomarker and therapeutical target to prevent malignant peripheral nerve sheath tumor growth and metastasis

  Teresa González-Muñoz, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Control groups received thrice-weekly oral administrations of vehicle and/or biweekly intraperitoneal injections of IgG (Jackson Immunoresearch; 10mg/Kg diluted in PBS). Tumor volume was measured as described above ...
• Endoglin, a novel biomarker and therapeutical target to prevent malignant peripheral nerve sheath tumor growth and metastasis

  Teresa González-Muñoz, et al., bioRxiv - Cancer Biology 2022

  Quote: ... the combination of both drugs or IgG control (Jackson ImmunoResearch). TRC105 and IgG were resuspended in PBS and used at a final concentration of 50 μg/ml ...
• Gigaxonin is required for intermediate filament transport

  Bhuvanasundar Renganathan, et al., bioRxiv - Cell Biology 2022

  Quote: ... and Rhodamine (TRITC) goat anti-rabbit antibody (Jackson ImmunoResearch, #111-025-003). For actin staining ...
• Tetraploidy-linked sensitization to CENP-E inhibition in human cells

  Koya Yoshizawa, et al., bioRxiv - Cell Biology 2022

  Quote: ... overnight at 4°C, and with fluorescence (Alexa Fluor 488, 568, 647)-conjugated secondaries (Jackson ImmunoResearch Laboratories or Abcam; 1:1000) overnight at 4°C at indicated dilutions ...
• Gigaxonin is required for intermediate filament transport

  Bhuvanasundar Renganathan, et al., bioRxiv - Cell Biology 2022

  Quote: ... Alexa-Fluor 647-conjugated donkey Anti-Mouse (Jackson ImmunoResearch, #715-605-151), and Rhodamine (TRITC ...
• Gigaxonin is required for intermediate filament transport

  Bhuvanasundar Renganathan, et al., bioRxiv - Cell Biology 2022

  Quote: ... Secondary antibodies used in this study were Rhodamine conjugated goat anti-mouse (Jackson ImmunoResearch, #115-025-003), Alexa-Fluor 647-conjugated donkey Anti-Mouse (Jackson ImmunoResearch ...
• Reciprocal regulation between cell mechanics and ZO-1 guides tight junction assembly and epithelial morphogenesis

  Alexis J. Haas, et al., bioRxiv - Cell Biology 2022

  Quote: ... The following affinity-purified and cross-adsorbed secondary antibodies from Jackson ImmunoResearch were used ...
• Long-term mitotic DNA damage promotes chromokinesin-mediated missegregation of polar chromosomes in cancer cells

  Marco Novais-Cruz, et al., bioRxiv - Cell Biology 2022

  Quote: ... and anti-mouse-HRP and anti-rabbit-HRP were used as secondary antibodies (1:5,000; Jackson ImmunoResearch Laboratories, Inc.).
• Misaligned chromosomes that satisfy the spindle assembly checkpoint are a strong predictor of micronuclei formation in dividing cancer cells

  Ana Margarida Gomes, et al., bioRxiv - Cell Biology 2022

  Quote: ... anti-sheep-HRP and anti-rat-HRP were used as secondary antibodies (1:5000; Jackson ImmunoResearch Laboratories, Inc.).
• SWIP mediates retromer-independent membrane recruitment of the WASH complex

  V. Dostál, et al., bioRxiv - Cell Biology 2022

  Quote: ... Horseradish Peroxidase goat anti-rabbit (Jackson ImmunoResearch, cat. no. 111-035-045, 1:10000) and Horseradish peroxidase bovine anti-goat (Jackson ImmunoResearch ...
• SWIP mediates retromer-independent membrane recruitment of the WASH complex

  V. Dostál, et al., bioRxiv - Cell Biology 2022

  Quote: ... donkey anti-rabbit IgG (H+L) secondary antibody-Alexa 488 (Jackson ImmunoResearch, cat. no. 711-545-152, 1:500) was used together with transferrin-Alexa Fluor 594 or with donkey anti-goat IgG (H+L ...
• SWIP mediates retromer-independent membrane recruitment of the WASH complex

  V. Dostál, et al., bioRxiv - Cell Biology 2022

  Quote: ... and Horseradish peroxidase bovine anti-goat (Jackson ImmunoResearch, cat. no. 805-035-180, 1:5000). For immunofluorescence ...
• SWIP mediates retromer-independent membrane recruitment of the WASH complex

  V. Dostál, et al., bioRxiv - Cell Biology 2022

  Quote: ... was used together with donkey anti-rabbit IgG (H+L) secondary antibody-Cy5 (Jackson ImmunoResearch, cat. no. 711-175-152, 1:500). Alternatively ...
• Targeted protein degradation reveals BET bromodomains as the cellular target of Hedgehog Pathway Inhibitor-1

  Meropi Bagka, et al., bioRxiv - Cell Biology 2022

  Quote: ... and incubated with appropriate secondary antibodies in blocking solution (1:500 Jackson ImmunoResearch) for 1h at room temperature ...
• SWIP mediates retromer-independent membrane recruitment of the WASH complex

  V. Dostál, et al., bioRxiv - Cell Biology 2022

  Quote: Secondary antibodies used for Western blot were Horseradish peroxidase goat antimouse (Jackson ImmunoResearch, cat. no. 115-035-146, 1:10000), Horseradish Peroxidase goat anti-rabbit (Jackson ImmunoResearch ...
• Dynamic states of cervical epithelia during pregnancy and epithelial barrier disruption

  Anne Cooley, et al., bioRxiv - Cell Biology 2022

  Quote: ... Donkey anti-chicken Alexa Fluor 488 (1:600, Jackson ImmunoResearch 703-545-155) conjugated secondary antibodies ...
• A Leaky Human Colon Model Reveals Uncoupled Apical/Basal Cytotoxicity in Early Clostridioides difficile Toxin Exposure

  Meryem T. Ok, et al., bioRxiv - Cell Biology 2022

  Quote: ... then incubated with Alexa Fluor 488-conjugated donkey anti-rabbit antibody (1:500, 711-545-152; Jackson ImmunoResearch, West Grove, PA) and bisbenzimide H 33258 (1:1000 ...
• Synaptotagmin-9 and Tomosyn-1 molecular complex regulates Stx1A SNAREs to inhibit insulin secretion from pancreatic β-cells

  Md Mostafizur Rahman, et al., bioRxiv - Cell Biology 2022

  Quote: ... for Western blotting and mouse Alexa Fluor 488 (Jackson ImmunoResearch, Cat#115-545-166) and rabbit Alexa Flour 647 (Jackson ImmunoResearch ...
• STIM1 signals through NFAT independently of Orai1 and SOCE to regulate breast cancer cell migration

  Ayat S. Hammad, et al., bioRxiv - Cell Biology 2022

  Quote: ... Both HRP-conjugated goat anti-rabbit-IgG and goat anti-mouse-IgG antibodies (1:5000) were purchased from Jackson ImmunoResearch Laboratories.
• Synaptotagmin-9 and Tomosyn-1 molecular complex regulates Stx1A SNAREs to inhibit insulin secretion from pancreatic β-cells

  Md Mostafizur Rahman, et al., bioRxiv - Cell Biology 2022

  Quote: ... and rabbit Alexa Flour 647 (Jackson ImmunoResearch, Cat# 111-605-003) for confocal imaging.
• A Concerted Mechanism Involving ACAT and SREBPs By which Oxysterols Deplete Accessible Cholesterol To Restrict Microbial Infection

  David B. Heisler, et al., bioRxiv - Cell Biology 2022

  Quote: ... or goat anti-rabbit IgG (Jackson ImmunoResearch) conjugated to horseradish peroxidase ...
• The ancestral ESCRT protein TOM1L2 selects ubiquitinated cargoes for retrieval from cilia

  Swapnil Rohidas Shinde, et al., bioRxiv - Cell Biology 2022

  Quote: ... 5% normal donkey serum (017-000-121; Jackson ImmunoResearch Laboratories), and 3% bovine serum albumin (BP1605-100 ...
• Quantitative super-resolution imaging of platelet degranulation reveals differential release of VWF and VWF propeptide from alpha-granules

  Maurice Swinkels, et al., bioRxiv - Cell Biology 2022

  Quote: ... or with secondary anti-Alpaca IgG-AF488 (Jackson ImmunoResearch, 1:400) for 15 minutes at RT ...
• Quantitative super-resolution imaging of platelet degranulation reveals differential release of VWF and VWF propeptide from alpha-granules

  Maurice Swinkels, et al., bioRxiv - Cell Biology 2022

  Quote: ... Internalized nanobodies were detected using goat anti-Alpaca IgG-AF488 (Jackson ImmunoResearch).
• The ancestral ESCRT protein TOM1L2 selects ubiquitinated cargoes for retrieval from cilia

  Swapnil Rohidas Shinde, et al., bioRxiv - Cell Biology 2022

  Quote: ... and then incubated with secondary antibodies (Jackson ImmunoResearch Laboratories) diluted in IF buffer for 30 min ...
• Pericyte Heterogeneity Identified by 3D Ultrastructural Analysis of the Microvessel Wall

  Hanaa Abdelazim, et al., bioRxiv - Cell Biology 2022

  Quote: ... Secondary antibodies applied included donkey anti-goat IgG conjugated to AlexaFluor647 (Jackson ImmunoResearch) and donkey anti-mouse IgG1 conjugated to AlexaFluor488 (Jackson ImmunoResearch).
• Meiotic DNA exchanges are promoted by proximity to the synaptonemal complex

  David E. Almanzar, et al., bioRxiv - Cell Biology 2022

  Quote: ... Cy3 AffiniPure Donkey anti-guinea-pig (1:500, Jackson Immunoresearch), 488 AffiniPure Donkey anti-mouse (1:500 ...
• Pericyte Heterogeneity Identified by 3D Ultrastructural Analysis of the Microvessel Wall

  Hanaa Abdelazim, et al., bioRxiv - Cell Biology 2022

  Quote: ... and donkey anti-mouse IgG1 conjugated to AlexaFluor488 (Jackson ImmunoResearch).
• IL-11 disrupts alveolar epithelial progenitor function

  Rosa K. Kortekaas, et al., bioRxiv - Cell Biology 2022

  Quote: ... in 1% (w/v) BSA 2% donkey serum (Jackson Immunoresearch, West Grove ...
• IL-11 disrupts alveolar epithelial progenitor function

  Rosa K. Kortekaas, et al., bioRxiv - Cell Biology 2022

  Quote: ... sections were incubated with the secondary antibody peroxidase-conjugated donkey anti-rabbit (Jackson Immunoresearch, #711-035-152, RRID: AB_10015282) diluted 1:500 in 1% BSA 2% donkey serum in PBS for 30 min at room temperature ...
• Meiotic DNA exchanges are promoted by proximity to the synaptonemal complex

  David E. Almanzar, et al., bioRxiv - Cell Biology 2022

  Quote: ... 594 AffiniPure Donkey anti-mouse (1:200, Jackson Immunoresearch), Goat anti-rabbit STAR RED (1:200 ...
• Meiotic DNA exchanges are promoted by proximity to the synaptonemal complex

  David E. Almanzar, et al., bioRxiv - Cell Biology 2022

  Quote: ... 594 AffiniPure Donkey anti-guinea-pig (1:200, Jackson Immunoresearch), 594 AffiniPure Donkey anti-mouse (1:200 ...
• Meiotic DNA exchanges are promoted by proximity to the synaptonemal complex

  David E. Almanzar, et al., bioRxiv - Cell Biology 2022

  Quote: ... 488 AffiniPure Donkey anti-rabbit (1:500, Jackson Immunoresearch), 594 AffiniPure Donkey anti-guinea-pig (1:200 ...
• Oocytes can repair DNA damage during meiosis via a microtubule-dependent recruitment of CIP2A-MDC1-TOPBP1 complex from spindle pole to chromosomes

  Jiyeon Leem, Jae-Sung Kim, Jeong Su Oh, bioRxiv - Cell Biology 2022

  Quote: ... Alexa Fluor-conjugated 488 (1:500, 115-545-146, Jackson ImmunoResearch), Alexa Fluor-conjugated 594 (1:500 ...
• Malonyl-CoA is an ancient physiological ATP-competitive mTORC1 inhibitor

  Raffaele Nicastro, et al., bioRxiv - Cell Biology 2022

  Quote: ... cells were stained with highly cross-adsorbed fluorescent secondary antibodies (Donkey anti-rabbit FITC, Donkey anti-mouse TRITC; both from Jackson ImmunoResearch) diluted 1:200 in BBT for 1 h ...