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Citations for Jackson ImmunoResearch :
451 - 500 of 4221 citations for 4 2 Hydroxyimino 2 4 methoxy phenyl ethyl piperazine 1 carboxylic acid tert butyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... and blocked with 1% BSA in carbonate buffer before being incubated overnight at 4°C with rabbit anti-sheep IgGs in carbonate buffer (Jackson ImmunoResearch, #313-005-003). 15 µL of cell lysate was incubated for 3 h at 4°C in the IgG-coated 384-well plates ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed 4 × 30s in a tris buffer solution and the secondary biotinylated mouse adsorbed donkey anti-rabbit antibody (Jackson ImmunoResearch, 711-065-152) applied to all sections and incubated for 30 min ...
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bioRxiv - Immunology 2021Quote: ... Cells were then blocked for 1 hour in PFN buffer (PBS without Ca2+ and Mg2+ and containing 10% FBS and 0.02% azide) with 2% normal goat serum (Jackson ImmunoResearch). Cells were incubated for 1 hour with the appropriate primary antibodies diluted in blocking medium ...
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bioRxiv - Bioengineering 2021Quote: ... we labeled transduced T cells with Alexa Fluor 647- labeled rabbit anti-mouse F(ab)2 antibody from Jackson ImmunoResearch Laboratories Inc ...
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bioRxiv - Microbiology 2021Quote: ... and for multiplex beadbased assay with anti-mouse IgG (Fc-specific) F(ab’)2 PE (Jackson ImmunoResearch, West Grove PA). Data were acquired on a Magpix instrument (Luminex ...
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bioRxiv - Immunology 2020Quote: ... then detected by Alexa Fluor 647 goat anti-human secondary antibody (2 μg/ml; 109-605-098, Jackson ImmunoResearch Laboratories). In tests of their specificity for binding RBC ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were incubated for 2 hours with a species-specific secondary antibody conjugated with green Alexa fluorophore (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Developmental Biology 2021Quote: ... After 3x washing in PBT (with 0.1% Tween 20) embryos were incubated for 2 hours at room temperature with secondary antibodies from Jackson Immunoresearch. Vectashield with DAPI or TSO was used as mounting medium.
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked for 2 hours at room temperature (RT) in 5% normal goat serum (Jackson ImmunoResearch, West Grove, PA). Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... and 36 µg rabbit anti-HA-tag (Cell Signalling Technology #3724) followed by fluorescently conjugated secondary antibodies in a 3:2 primary:secondary molar ratio (Jackson ImmunoResearch). Samples were incubated in EasyIndex (LifeCanvas Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... AffiniPure F(ab’)2-goat anti-rat IgG (H+L) Alexa 488 (Jackson ImmunoResearch Labs Cat# 112-546-003, RRID:AB_2338364), F(ab’)2-goat anti-mouse IgG CF568 (Biotium Cat# 20109 ...
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bioRxiv - Biophysics 2023Quote: ... permeabilization and and blocking were performed with PBS (with 0.4% (v/v) Triton X-100%) and 2% immunoglobulin G-free BSA (Jackson ImmunoResearch) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... plates were coated with either 2 µg/ml of donkey anti-mouse IgG (Affinipure; Jackson ImmunoResearch Laboratories, West Grove, PA), carbonate buffer (0.0875 M Na2CO3 ...
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bioRxiv - Bioengineering 2023Quote: ... and a 1000x dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson Immunoresearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Immunology 2023Quote: ... the TCR was stimulated by addition of 15 μg/ml of the CD3 antibody clone UCHT1 or alternatively by restimulation with CD3 mAbs (TR66 clone) and crosslinking by 1.3 μg/mL anti-mouse IgGF(ab)’2 fragments (Jackson ImmunoResearch). The ratio of Ca2+-bound Indo-1 versus Ca2+-free Indo-1 was analyzed using FlowJo 9 software (Beckman Coulter).
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bioRxiv - Immunology 2024Quote: ... B cells were stimulated with 10 μg/mL polyclonal F(ab’)2 goat anti-mouse IgM (anti-μ) (Jackson ImmunoResearch) or 5 μg/mL anti-IgDa (clone AMS 9.1 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with 2 μg/mL goat anti-human IgG (H+L) Alexa647 (Jackson ImmunoResearch, Cat# 109-605-003) for 30 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... After extensive washes with 2× SSC at 37°C the probe was revealed using Cy-conjugated streptavidin (Jackson Immunoresearch Laboratories), followed by immunostaining as described above.
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bioRxiv - Cell Biology 2020Quote: ... Blotted proteins were incubated with corresponding primary antibodies at 4°C overnight followed by incubation with horseradish peroxidase conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA, USA). Amersham ECL Western blotting detection reagent (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4°C with the primary antibodies and then with the corresponding horseradish peroxidase-conjugated secondary antibodies (Jackson Immunoresearch, West Grove, PA, USA). All membranes were revealed using ECL chemiluminescence kit according to the manufacturer’s instructions (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated for ∼ 2 h at room temperature with the appropriate AlexaFluor or Dylight 488/568/647 conjugated secondary antibodies (ThermoFisher / Jackson ImmunoResearch) and counterstained with DAPI ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed and Peroxidase-AffiniPure F(ab’)2 Fragment Goat anti-mouse IgG (111-036-047, Jackson ImmunoResearch, USA) diluted to 1:20,000 in blocking buffer was added to every well and incubated for 1 hr using a plate shaker set to 450 rpm at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Detection reagents consisted of (i) goat anti-human IgG (Fcγ specific) F(ab’)2 AF594 conjugate (Jackson ImmunoResearch 109-586-170), (ii ...
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bioRxiv - Cell Biology 2021Quote: Mouse splenic B-cells were incubated with F(ab’)2 goat-anti-mouse IgM+G (10 μg/ml, Jackson ImmunoResearch Laboratories) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 2 hours at room temperature with Alexa Fluor-conjugated secondary antibodies (Thermo Fisher Scientific or Jackson Immunoresearch). Nuclei were labeled with DAPI ...
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bioRxiv - Immunology 2020Quote: ... Cells were then fixed at indicated time points in 2% paraformaldehydestained with fluorescently conjugated antibodie Fab fragments targeting IgM (Jackson Immunoresearch) and analysed by flow cytometry ...
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bioRxiv - Cell Biology 2021Quote: ... We used the following secondary antibodies: F(ab’)2 conjugates with FITC Donkey Anti-Guinea Pig IgG (H+L) (Jackson ImmunoResearch 706-096-148 ...
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bioRxiv - Biochemistry 2022Quote: ... The horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG and phycoerythrin (PE)-anti-rabbit F(ab’)2 antibody was obtained from Jackson ImmunoResearch Laboratories ...
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bioRxiv - Immunology 2022Quote: Flat-bottom plate were coated with 20 μg/ml AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L)mouse (Jackson Immunoresearch) in PBS for two hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... and IgG responses were detected with goat anti-human IgG conjugated with horseradish peroxidase (anti-IgG F[ab]2 for F[ab]) (Jackson ImmunoResearch). IgM and IgA anti-OSP reactivity of plasma was measured using goat anti-human IgM and IgA conjugated with horseradish peroxidase (Jackson ImmunoResearch) ...
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bioRxiv - Microbiology 2020Quote: ... Purity of the F[ab] fragments was assessed by Western blot analysis with 100 ng of sample per well and direct probing with anti-IgG F[ab]2 and anti-IgG Fc conjugated to horseradish peroxidase (Jackson ImmunoResearch).
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies against whole Ig or Ig light-chain conjugated to horseradish peroxydase or Cyanine-2 and −3 were purchased from Jackson ImmunoResearch. Phalloidin-Fluoprobes647 (FP-BA0320 ...
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bioRxiv - Neuroscience 2021Quote: ... RRID: AB_2532109) in PBS with 0.5% Triton X-100 (MilliporeSigma) and 2% normal donkey serum (Jackson ImmunoResearch, West Grove, PA, USA). Sections were then washed in PBS and incubated for 90 min at room temperature in Alexa Fluor 488- or 594-conjugated donkey anti-mouse IgG (1:250 ...
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bioRxiv - Immunology 2021Quote: ... Cy-5-conjugated donkey anti-rabbit IgG and Alexa fluor-488 F(ab’)2 fragment goat anti-mouse IgG were purchased from Jackson ImmunoResearch Laboratories (Cambridgeshire ...
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bioRxiv - Immunology 2020Quote: ... Allophycocyanin-conjugated F(ab′)2 goat anti-mouse or donkey anti-human IgG (H+L) secondary antibodies were purchased from Jackson ImmunoResearch Laboratories (West Grove ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The secondary antibody peroxidase-conjugated AffiniPure F (ab’)2 Fragment Goat Anti-Mouse IgG (H+L) was purchased from Jackson ImmunoResearch Laboratories Inc ...
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bioRxiv - Immunology 2021Quote: ... AF647 Goat anti-human IgG(H+L) F(ab’)2 fragment antibody was purchased from Jackson ImmunoResearch (West Grove, PA, USA). Anti-SARS-CoV-2 Coronavirus Spike protein (subunit 1 ...
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bioRxiv - Immunology 2021Quote: ... Isolated cells were then cultured in vitro in the presence or absence of 5 mg/ml anti-IgM F(ab)2 (Jackson Immunoresearch) and/or 5 mg/ml anti-CD40 (BioXCell ...
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bioRxiv - Immunology 2021Quote: ... 10 µg/ml biotin-SP AffiniPure F(ab’)2 fragment goat anti-mouse IgM (biotinylated anti-IgM, Jackson ImmunoResearch Laboratories, Inc.), 1 µg/ml recombinant murine CD40L (CD40L ...
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bioRxiv - Immunology 2020Quote: ... AF647 Goat anti-human IgG(H+L) F(ab’)2 fragment antibody was purchased from Jackson ImmunoResearch (West Grove, PA, USA). Anti-SARS-CoV-2 Coronavirus Spike protein (subunit 1 ...
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bioRxiv - Immunology 2021Quote: ... and recombinant mouse IL21R Fc Chimera (600x; R&D) followed by PE-labeled affinity-purified F(ab’)2 fragment of goat anti-human Fcγ (Jackson ImmunoResearch). For detection of phosphorylated antigens ...
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bioRxiv - Neuroscience 2022Quote: ... or Alexa Fluor® 647 AffiniPure Donkey Anti-Rabbit IgG (H+L) (2 µg/ml, Jackson ImmunoResearch, cat #711-605-152) for super-resolution experiments ...
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bioRxiv - Cell Biology 2024Quote: ... F(ab’)2 conjugates with fluorescein isothiocyanate donkey anti-guinea pig immunoglobulin G (IgG) (heavy and light, H + L) (Jackson ImmunoResearch 706-096-148 ...
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bioRxiv - Immunology 2023Quote: ... and the IgM assay a donkey anti-mouse IgM F(ab’)2 (Jackson ImmunoResearch, 715-476-020, labeled with DyLight 405), both at a final ...
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bioRxiv - Immunology 2023Quote: ... Bound ICs were detected using a PE-conjugated goat anti-human IgG F(ab’)2 fragment at 0.5 μg/ml (Jackson ImmunoResearch Laboratories) on a BD FACSCanto II flow cytometer ...
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bioRxiv - Neuroscience 2023Quote: ... slides were incubated for 2 h in the secondary antibody donkey anti-rabbit Cy3 (711-165-152) purchased from Jackson ImmunoResearch and used at a 1:500 dilution ...
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bioRxiv - Cancer Biology 2022Quote: CAR expression on T cells was analyzed by co-incubating cells with a Biotin-SP (long spacer) AffiniPure F(ab’)2 Fragment Goat Anti-Mouse IgG antibody (Jackson ImmunoResearch) for 30 min at 4°C and R-phycoerythrin (PE)-conjugated streptavidin (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2023Quote: ... were used for western blot and highly cross-adsorbed IgG F(ab′)2 secondary antibodies conjugated to Alexafluor 594 or Alexafluor 647 (Jackson Immunoresearch) were used for immunofluorescence.
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bioRxiv - Microbiology 2023Quote: ... with the exception that primary and secondary antibodies were replaced by sera diluted in TBS/2% skimmed milk/20% sheep serum and an HRP-labeled goat anti-rabbit IgG (Jackson Immunoresearch), respectively.
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bioRxiv - Microbiology 2023Quote: ... We then incubated cells with a PE-conjugated donkey anti-mouse F(ab’)2 fragment (Jackson ImmunoResearch Cat #: 715-116-150) for 30 min at 4 °C ...