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Citations for Beckman :
1 - 50 of 359 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: Detection of apoptotic TCam-2 cells was performed 48 h after transfection of TCam-2 cells with control and KIF18A siRNA constructs using an Annexin V-FITC Apoptosis Detection Kit (Beckman Coulter), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Quality Control beads (Beckman Coulter) were used daily to check and standardize instrument performances ...
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bioRxiv - Immunology 2023Quote: ... quality controls metrics were checked using quality control beads (CytoFLEX Daily QC Fluorospheres; Beckman Coulter; B53230). Data were compensated using single positive stained cells ...
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bioRxiv - Cell Biology 2022Quote: ... along with corresponding 2D controls were analysed by Beckman Coulter MoFlow Astrios Cell Sorter (Beckman Coulter Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... The isotype control (FITC-labelled anti-CD83, Beckman Coulter) was also used to rule out nonspecific binding ...
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bioRxiv - Microbiology 2022Quote: ... An isotype control (FITC-labelled anti-CD83, Beckman Coulter) was used to evaluate possible nonspecific binding to epithelial cells ...
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bioRxiv - Neuroscience 2021Quote: Transfection efficiency was evaluated by flow cytometry (CytoFLEX, Beckman Coutler) using a 488 nm laser ...
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bioRxiv - Immunology 2019Quote: ... Beckman Coulter 4C© Plus Tri-Pack Cell Controls (Beckman Coulter) were used to confirm instrument accuracy and precision.
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bioRxiv - Physiology 2021Quote: ... 2mM EDTA or with control isotypes IgG1 PE (A07796, Beckman Coulter) and biotinylated goat IgG (BAF108 ...
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bioRxiv - Cancer Biology 2023Quote: ... Quality control was evaluated using CytoFLEX Daily QC Fluorospheres (Beckman Coulter).
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bioRxiv - Microbiology 2022Quote: ... single stain controls for compensation were prepared using VersaComp beads (Beckman Coulter) and ArC Amine Reactive Compensation beads (ThermoFisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... with kits and controls supplied by Beckmann Coulter (Beckman Coulter, Lismeehan, Ireland). Free fatty acid was measured on the AU-400 using a kit from Wako (Wako Chemical Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... Corresponding isotype controls were as follows: IgG1 Mouse-PE (A0779, Beckman Coulter), IgG2a Mouse-PE (A09142 ...
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bioRxiv - Systems Biology 2021Quote: ... the transfection efficiency was monitored by flow cytometry (Gallios Flow cytometer, Beckman Coulter) of GFP-expressing cells based on mean fluorescence intensities in the FL-1 channel ...
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bioRxiv - Immunology 2022Quote: ... Quality control was performed daily using the recommended CytoFLEX daily QC fluorospheres (Beckman Coulter). No modification to the compensation matrices was required throughout the study ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 0.5 μl of appropriate compound stock or control was added using Biomek NXp (Beckman Coulter) and incubated at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Quality control of the cytometer was assessed daily using CytoFLEX Daily QC Fluorospheres (Beckman Coulter B53230). Data were collected by CytExpert (Beckman Coulter ...
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bioRxiv - Immunology 2019Quote: ... and subjected to cell quality control using Vi-CELL XR cell counter (Beckman Coulter, Brea, CA). All of the processed B cells had cell viability >90% ...
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bioRxiv - Immunology 2021Quote: ... Samples from COVID-19 patients and controls were assessed on a Cytoflex LX cytometer (Beckman Coulter), verified through daily calibration with CytoFLEX Daily QC Fluorospheres (Beckman Coulter) ...
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bioRxiv - Cell Biology 2021Quote: ... Quality control of the cytometer was assessed daily using CytoFLEX Daily QC Fluorospheres (Beckman Coulter, #B53230). Data were collected by CytExpert (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: ... Quality control of the cytometer was assessed weekly using CytoFLEX Daily QC Fluorospheres (Beckman Coulter B53230). The fluorescence threshold was set for the APC laser using a blank sample ...
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bioRxiv - Biophysics 2021Quote: ... 24h after transfection cells media (9mL) were filtered before performing VLPs purification by ultracentrifugation (SW41Ti rotor (Beckman) 29 000rpm ...
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bioRxiv - Microbiology 2024Quote: ... the control sample and drug-treated sample were analyzed on a Cytoflex S flow cytometer (Beckman Coulter) equipped with a 96-well sampler ...
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bioRxiv - Cancer Biology 2024Quote: ... the appropriated amounts of Sulfopin or DMSO (control) were transferred using Echo Liquid Handler 550 (Beckman, USA) into white/white 384-well TC plate (Greiner ...
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bioRxiv - Biophysics 2023Quote: ... then harvested 72 hours post transfection by centrifugation at 15,970 g in a JLA 8.1 rotor (Beckman Coulter). Cell pellets were snap-frozen in liquid nitrogen and stored at −80°C until use.
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bioRxiv - Cell Biology 2021Quote: ... Viral supernatant was harvested 36 and 48 h post-transfection and concentrated using ultracentrifugation (Beckman Coulter, Optima XPN-80) for 1.5 h at 19 000 rpm and 4 °C and then stored at −80o C ...
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bioRxiv - Immunology 2024Quote: ... The LS 13320 machine was verified before use using Garnet G15 control particles provided by the manufacturer (Beckman-Coulter).
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bioRxiv - Biochemistry 2024Quote: ... and mid controls dispensed in identical sets across the plate using the Echo 550 dispenser (Beckman Coulter, Brea, CA) for compounds/DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... Lentiviral supernatant was collected 48 hours after transfection and centrifuged for 2 hours by ultracentrifugation at 25,000 RPM at 4°C (Beckman Coulter). Pellets were resuspended in DPBS and frozen in -80°C until used ...
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bioRxiv - Cell Biology 2022Quote: ... to obtain a high percentage of transfected cells 24 h post-transfection GFP expressing cells were FACS sorted by MoFlo Astrios (Beckman). Following sorting total RNA was prepared ...
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bioRxiv - Biophysics 2023Quote: ... Virus-containing supernatants were collected at 48 hours and 72 hours after transfection and concentrated using ultracentrifugation (Ultraclear, Beckman 344058). The transduction capacity of the viral vector product was confirmed by culturing non-transfected HEK293T cells with the virus after 24 hours and assessing via fluorescent imaging.
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Genomic dissection and mutation-specific target discovery for breast cancer PIK3CA hotspot mutationsbioRxiv - Genomics 2024Quote: ... Cell counts and viability were measured 72 hours following transfection using a Vi-CELL BLU cell viability analyzer (Beckman Coulter).
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bioRxiv - Cancer Biology 2020Quote: ... isotype controls for intracellular stains and subjected to post-acquisition compensation using VersaComp Antibody Capture Bead Kit (Beckman Coulter #B22804). Data was analyzed using Kaluza Software (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2022Quote: ... Stained cells and fluorescence minus one controls were then resuspended in SB + 1:1000 DNase + 1:1000 Draq7 (Beckman Coulter) as a live/dead stain ...
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bioRxiv - Genomics 2020Quote: ... Lentiviral supernatant was collected at 48 and 72hrs post-transfection and subsequently concentrated by ultracentrifugation (24,000 rpm, 4°C, 2hrs) (Beckman Coulter SW32).
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bioRxiv - Immunology 2021Quote: ... reaction using naive C57BL/6OlaHsd mice genomic DNA for the internal control or donor AAV plasmid for the Donor sample and purified using AMPure XP beads (Beckman Coulter) at a 1:1 ratio ...
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bioRxiv - Cancer Biology 2022Quote: ... vehicle control or compounds from the small molecule library (Supplementary Table 4) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were then incubated in an IncuCyte Zoom (Sartorius ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 nM leptomycin b, vehicle control or library compounds (Supplementary Tables 2, 3) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher) ...
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bioRxiv - Microbiology 2020Quote: Larger amounts of MV-ZIKV and control MV supernatants were spun through a 20% sucrose cushion in an SW32 Ti rotor (Beckman, Inc.) at 25,000 rpm for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... with anti-Met-PE (clone eBioclone 7, eBiosciences) or isotype control (clone eBRG1, eBiosciences) or permeabilized (IntraPrep Permeabilization kit, Beckman Coulter) before staining (total Met) ...
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bioRxiv - Genomics 2020Quote: ... CD45RO-ECD (Beckman Coulter; Cat# IM2712U), CD123-PE/Cy5 (BD Biosciences ...
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bioRxiv - Genomics 2024Quote: ... 0.6x AMPure beads (Beckman, cat. A63880) were used to eliminate large fragments ...
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bioRxiv - Cell Biology 2020Quote: ... tSETD2-Flag plasmid was transfected into HEK293 Freestyle cells with FectoPRO transfection reagent (116-010) and cells were harvested 36-48 hours later at 5,000 rpm for 15 mins (Beckman JLA 8.1, 363563). The pellet was suspended in lysis buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: tSETD2-FLAG was transfected into HEK 293 Freestyle cells with FectoPRO transfection reagent (Polyplus, 10118-444) and cells were harvested 48 hours later at 5K rpm for 15 mins (Beckman JLA 8.1).The pellet was suspended in lysis buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Viral particles in the resulting supernatants were collected 48hrs after transfection and concentrated by ultracentrifugation (90 minutes at 48960g on Beckman SW28 rotor). Titers used in all experiments range from 0.5-1.5 × 109TU/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Collection of cells and media for AAV harvesting began 72 hours following transfection before iodixanol gradient ultracentrifugation purification using a Type 70 Ti Fixed-Angle Titanium Rotor (Beckman-Coulter, 337922). Titer was calculated using digital droplet PCR (ddPCR ...
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bioRxiv - Neuroscience 2023Quote: ... the puromycylated GF and vehicle treated control GF were loaded onto 60% sucrose pads in open top polycarbonate centrifuge tubes (Beckman Coulter #343776) filled to top with RNA granule buffer and centrifuged for 45 min at 50,000 x g at 4°C in a Sorvall table-top ultracentrifuge with a TLA 100 fixed angle rotor ...
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Fetal estrogens are not involved in sex determination but critical for early ovarian differentiationbioRxiv - Developmental Biology 2021Quote: ... Anti Müllerian hormone levels were determined in 50 μl aliquots of serum samples by using an ELISA kit (AMH GenII ELISA, with AMH Gen II calibrators and controls, Beckman Coulter, Villepinte, France) as previously described (Bourdon et al ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We pooled 1.5 µl of each indexed library (including blanks and museum controls) and performed size selection with AMPure XP beads (Beckman Coulter, IN, USA) for fragments of approximately 100-500 bp in length ...
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bioRxiv - Cancer Biology 2021Quote: ... Ultracentrifuge polyallomer tubes (Beckman Coulter, Cat #331372) were marked halfway and ~5.5 mL 10% sucrose polysome gradient buffer (20 mM Tris HCl pH 7.5 ...