Labshake search
Citations for Beckman :
351 - 400 of 428 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2019Quote: ... Agencourt AMPure XP PCR purification kit (5 ml Beckman Coulter Part No. A63880; 60 ml Beckman Coulter Part No. A63881) was used ...
-
bioRxiv - Immunology 2021Quote: ... The first-strand synthesis reaction was purified using the Ampure Size Select Magnetic Bead Kit at a ratio of 0.6X (Beckman Coulter). Following ...
-
bioRxiv - Immunology 2021Quote: ... The first PCR reaction was purified using the AMPure Size Select Magnetic Bead Kit at a ratio of 0.6X (Beckman Coulter). Amplicon libraries were then prepared according to the Pacific Biosciences Multiplex SMRT Sequencing protocol and sequenced on a Pacific Biosciences Sequel platform (Pacific Biosciences ...
-
bioRxiv - Genomics 2022Quote: Pieces of caudal fin sampled from 463 fish (288 from LB line and 175 from LC line) were sent to Gentyane genotyping platform (INRAE, Clermont-Ferrand, France) for DNA extraction using the DNAdvance kit from Beckman Coulter following manufacturer instructions and genotyping using the newly constructed HD SNP array.
-
bioRxiv - Microbiology 2022Quote: ... The rest of the DNA purification process was performed using a GenFind v2 DNA extraction kit (Beckman Coulter, Indianapolis, U.S.) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... The rest of the DNA purification process was performed using a GenFind v2 DNA extraction kit (Beckman Coulter, Indianapolis, U.S.) following the manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The upper aqueous phase was further processed for total RNA extraction using the Agencourt RNAdvance Tissue Kit (Beckman Coulter, Germany) on a KingFisher Flex Purification System (Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2023Quote: ... PCR products were purified with the Qiagen MinElute PCR Purification Kit and AMPure XP beads (Beckman Coulter, Cat. No. A63880) and resuspended in ultrapure nuclease-free distilled water ...
-
bioRxiv - Genetics 2023Quote: ... clean-up and flowcell loading following the protocol for SQK-LSK112 sequencing kit (Version GDE_9141_v112_revC_01Dec2021) with the following modifications: Incubation with and elution from AMPure XP beads (Beckman Coulter, Cat. No. A63882) was extended to 30 minutes each ...
-
bioRxiv - Microbiology 2023Quote: Subsequent second-strand synthesis was performed using the Sequenase 2.0 kit (Thermo #70775Z1000UN) and amplicons cleaned with 1.8 × AMPure XP magnetic beads (Beckman Coulter #A63882). Purified cDNA samples were stored at −20°C until library preparation.
-
bioRxiv - Evolutionary Biology 2023Quote: We extracted DNA from fin clips collected from wild-caught fish using the Agencourt DNAdvance kit (Beckman Coulter, Brea, California). We used half-reactions but otherwise followed the manufacturer’s instructions for DNA extraction ...
-
bioRxiv - Pathology 2024Quote: RNA from rat liver tissue was snap frozen in RNAlater and lysed using lysis buffer (RNAdvanced Kit, A32646, Beckman Coulter), 20µL Proteinase K on a TissueLyser II (Qiagen ...
-
bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using 2S Plus DNA Library Kit adapters (Swift Biosciences) and size-selected using SPRIselect beads (Beckman Coulter) before being amplified and sent for paired-end sequencing on the NovaSeq 6000 (150 bp reads).
-
bioRxiv - Molecular Biology 2024Quote: ... The initial PCR products of rbcL F3R3 were purified using an Agencourt AMPure XP kit (Beckman Coulter, Fullerton, CA, USA). To construct the DNA libraries for the second PCR ...
-
bioRxiv - Microbiology 2019Quote: ... The first and second PCR reaction products were purified using AMPure XP PCR product cleanup and size selection kit (Beckman Coulter), following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2019Quote: ... a purification step was performed using MiniELute Qiagen kit and size separation on Agencourt AMPure XP beads (A63882, Beckman Coulter, Inc) to remove PCR Nextera primers.
-
bioRxiv - Genetics 2019Quote: ... Three PCR reactions were pooled and the PCR product was purified using the Agencourt AMPure XP PCR purification kit (Beckman Coulter) and sequenced using MiSeq (Illumina).
-
bioRxiv - Cancer Biology 2020Quote: ... Amplified libraries were purified with the Qiagen PCR clean-up MinElute kit and size selected for fragments less than 600 bp using the Agencourt AMPure XP beads (Beckman Coulter). Libraries were quality controlled by Qubit and Agilent DNA Bioanalyzer analysis ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA was prepared using Takara Clontech SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Cat.No.:634890) using 8 cycles of amplification and purified using Agencourt AMPure XP beads (Beckman Coulter, A63880). 1 ng of each sample cDNA was used for library preparation ...
-
bioRxiv - Microbiology 2020Quote: ... The resulting cDNA was PCR-amplified to about 10-20 ng/μl using a high-fidelity DNA polymerase followed by purification using the Agencourt AMPure XP kit (Beckman Coulter). The cDNA samples were pooled with ratios according to the RNA concentrations of the input samples and a size range of 200-550 bp was eluted from a preparative agarose gel ...
-
bioRxiv - Microbiology 2021Quote: ... PCR products from mouse samples were gel purified whereas PCR products from ferret samples were purified with paramagnetic beads using the AMPure XP for PCR Purification kit (Beckman Coulter). Purified PCR products were used in a second PCR reaction for incorporating sample-specific 5’-end indexes and additional Illumina sequencing adapters (Supplementary Table 2) ...
-
bioRxiv - Microbiology 2020Quote: ... The first and second PCR reaction products were purified using AMPure XP PCR product cleanup and size selection kit (Beckman Coulter), following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... The presence or absence of fecal occult blood in fresh pellets was determined using a Hemoccult blood testing kit (Beckman Coulter).
-
bioRxiv - Cell Biology 2021Quote: ... or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) and Lin−Sca-1+c-Kit+ (LSK) cells were sorted on MoFlo Astrios EQ (Beckman Coulter). Quantification of CD71 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were tagged using barcodes NB01 to NB05 of the Native Barcoding Expansion 1-12 PCR-free kit (EXP-NBD104, ONT) and purified using Agencourt AMPure XP beads (Beckman Coulter). The barcoded samples were quantified using a Qubit fluorometer and pooled equimolarly to reach 700 ng in 65 μL ...
-
bioRxiv - Immunology 2022Quote: ... nested PCR of the TCR locus was performed following the purification of PCR product by an AM Pure XP kit (Beckman Coulter) at a 0.7:1 ratio of beads to sample and eluted with 20 μL of 10 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Genomics 2019Quote: ... The resulting PCR reactions were pooled and purified (first using the minElute PCR purification kit of Qiagen, followed by an extra purification step using AMPureXP beads of Beckman Coulter), and the amplified cDNA quantified on a BioAnalyzer High Sensitivity Chip (Agilent) ...
-
bioRxiv - Developmental Biology 2019Quote: ... with a final double (0.65 x −1 x bead volume) size selection step using a SPRIselect reagent kit (Beckman Coulter, B23318). The libraries were indexed (New England BioLabs ...
-
bioRxiv - Developmental Biology 2019Quote: Detection of apoptotic TCam-2 cells was performed 48 h after transfection of TCam-2 cells with control and KIF18A siRNA constructs using an Annexin V-FITC Apoptosis Detection Kit (Beckman Coulter), according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... The amplification products were checked using gel electrophoresis and were purified using the Agencourt AMPure XP Kit (Beckman Coulter, CA, USA). The purified products were the indexed in the 16S V4–V5 library ...
-
bioRxiv - Genetics 2021Quote: ... The fragment analysis was carried out using capillary electrophoresis in the Beckman Coulter CEQ8000 using the DNA Size Standard Kit-400 (Beckman Coulter) with 1.5 μl PCR product diluted in 20 μl formamide.
-
bioRxiv - Cell Biology 2020Quote: ... with anti-Met-PE (clone eBioclone 7, eBiosciences) or isotype control (clone eBRG1, eBiosciences) or permeabilized (IntraPrep Permeabilization kit, Beckman Coulter) before staining (total Met) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The amplified DNA was purified using a Qiagen MinElute PCR Purification Kit and primer dimers (<100 bp) were removed using AMPure beads (Beckman Coulter). ATAC-Seq was performed with two biological repeats to ensure the robustness of the data sets ...
-
bioRxiv - Microbiology 2021Quote: ... The first and second PCR reaction products were purified using AMPure XP PCR product cleanup and size selection kit (Beckman Coulter), following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... The library preparation was completed by ligation of adapters from the SQK-LSK109 kit (Oxford Nanopore Technologies) and cleaned up with AMPure XP beads (Beckman Coulter) before preparation for sequencing ...
-
bioRxiv - Microbiology 2020Quote: Library preparation was performed using Illumina NexteraXT™ DNA Sample Prep Kit per manufacturer’s protocol followed by AMPure XP (Beckman Coulter) purification ...
-
bioRxiv - Cancer Biology 2020Quote: ... 200ng of total RNA was first treated to remove the rRNA and then purified using the Agencourt RNA Clean XP Kit (Beckman Coulter) prior to analysis with the Agilent RNA 6000 Pico Chip to confirm rRNA removal ...
-
bioRxiv - Immunology 2022Quote: ... The presence or absence of fecal blood in fresh pellets was determined by macroscopic observation or by applying wet fecal samples to detection tabs from a Hemoccult blood testing kit (Beckman Coulter).
-
bioRxiv - Cancer Biology 2022Quote: ... using MiSeq V2 300 cycle reagent kit after the amplified library was purified with AMPure XP beads (Beckman Coulter, CA, USA) (Illumina ...
-
bioRxiv - Microbiology 2022Quote: Samples were subjected to 750 μl of lysis buffer supplied with the GenFind V2 DNA extraction kit (Beckman Coulter, Indianapolis, U.S.). The suspension was vortexed for 10 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... stained the cells by a Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, A211-01) and detected cells distribution using a Beckman Coulter Gallios (Beckman Coulter). The result was analyzed by Kaluza Analysis 2.1 (Beckman Coulter).
-
bioRxiv - Microbiology 2023Quote: Samples were subjected to 750 μl of lysis buffer supplied with the GenFind V2 DNA extraction kit (Beckman Coulter, Indianapolis, U.S.). The suspension was vortexed for 10 min ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The PCR amplicons of the samples were then pooled after a purification and concentration equalization process with the AMPureXP Kit (Beckman Coulter). The libraries were processed in an Illumina MiSeq v2 (500 cycles and paired-end).
-
bioRxiv - Cell Biology 2023Quote: ... RNA was processed using a NEBNext Ultra II Directional RNA library Prep Kit for Illumina paired with a Poly(A) mRNA magnetic isolation module and AMPure XP SPRIselect beads (Beckman Coulter). Libraries were sequenced using a NextSeq 500 High Output sequencer (Illumina ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were purified using Qiagen MinElute PCR purification kit and size-selected (∼30-280 bp) using Ampure XP beads (Beckman Coulter). Reporter plasmid libraries were made by cloning amplified ATAC fragments into AgeI-HF- and SalI-HF-linearized pSTARR-seq plasmid using InFusion HD cloning kit (Takara ...
-
bioRxiv - Microbiology 2024Quote: ... The PCR products from thew first PCR were individually purified using 0.6X volumes of AMPure XP magnetic beads (AMPure XP beads kit / Beckman coulter, USA), followed by a second PCR reaction to attach the dual indices (S5 and N7 ...
-
bioRxiv - Immunology 2023Quote: ... Final PCR reaction was then purified with a MinElute PCR Purification Kit followed by size-selection (150bp-1000bp using Ampure XP beads, Beckman Coulter). Sequencing was performed using a MGISEQ-2000 (BGI Tech Solutions) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The resulting cDNA was PCR-amplified to about 10–20 ng/μl using a high-fidelity DNA polymerase and purified with the Agencourt AMPure XP Kit (Beckman Coulter Genomics). The cDNA samples were pooled based on the ratios according to the RNA concentrations of the input samples ...
-
bioRxiv - Biochemistry 2019Quote: ... low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) using commercially-available kits (Beckman-Coulter, CITY, STATE, USA). Serum insulin concentration was determined using an electrochemiluminescence-based assay (Immulite 2000 ...
-
bioRxiv - Microbiology 2019Quote: ... The cDNA pool was size fractionated in the size range of 200 to 600 bp using a differential cleanup with the Agencourt AMPure kit (Beckman Coulter Genomics). Aliquots of the cDNA pools were analyzed by capillary electrophoresis (Shimadzu MultiNA microchip) ...