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Citations for Beckman :
1 - 50 of 787 citations for Vitamin K epoxide reductase complex subunit 1 VKORC1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Ribosomal subunits were pelleted using a TLA100.3 rotor (Beckman Coulter, 349490) at ∼300,000 xg at 4 °C for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... k factor 81.3 (Beckman Coulter). RNA was isolated from the pelleted samples with a miRNeasy micro kit (Qiagen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The FITC fluorescence signals demonstrating the antibody-receptor complex on the cell surface were quantified by flow cytometry (CytoFLEX, Beckman Coulter). Relative expression levels of GPR1 mutants were represented according to the fluorescence signals.
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bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Subunit Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Subunit Buffer in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
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bioRxiv - Immunology 2023Quote: ... The fluorescence signals demonstrating the antibody-receptor complex on the cell surface were quantified by flow cytometry (CytoFLEX, Beckman Coulter, Brea, CA).
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bioRxiv - Immunology 2023Quote: ... The fluorescence signals for the antibody-receptor complex on the cell surface were then quantified by flow cytometry (CytoFLEX, Beckman Coulter, Brea, CA). Data were analyzed using FlowJo™ v10.8 Software (BD Life Sciences) ...
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bioRxiv - Biophysics 2024Quote: ... The subunits were then separated by ultracentrifugation in an SW32 rotor (Beckman Coulter, Catalog Number 369694) at 22,000 rpm for 17 hours at 4 °C.
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bioRxiv - Genomics 2023Quote: ... followed by the incubation with proteinase K for 1 hour and purified by AMPure XP (Beckman Coulter).
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bioRxiv - Immunology 2020Quote: ... CD103 (APC, B-Ly7, eBioscience, or biotin, 2G5, Beckman Coulter ...
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bioRxiv - Bioengineering 2024Quote: ... The absorption spectrum of the resulting phage-FAM complex was measured using a DU800 (Beckman) spectrophotometer.
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bioRxiv - Biophysics 2022Quote: ... the 30S and 50S subunits were separated on a 15-35% (w/v) sucrose gradient in dissociation buffer on a SW-32 rotor (Beckman-Coulter) spun at 28,000 rpm (97,000 xg ...
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bioRxiv - Cell Biology 2020Quote: ... EVs were ultracentrifuged (Type 45 Ti rotor, k-Factor 133, Beckman Coulter) with 100000 g for 90 min 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mg/ml Proteinase K) and normal phase 2x AMPureXP (Beckman Coulter, #A63881) bead clean-up ...
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bioRxiv - Biochemistry 2023Quote: The oligomeric state of mKu-DNA complex were assessed by determining sedimentation velocity using Optima AUC (Beckman Coulter). DNA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... fixed angle Type 45 Ti rotor (k factor = 133; Beckman Coulter, Miami, FL, USA), at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Then 200nL of 5ug/mL of MHCI complex sample were dispensed from the Echo qualified 384- well source plate (Beckman Coulter Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... using anti-HIV-1 p24-PE antibody (#6604667, 1:1000 dilution) purchased from Beckman Coulter ...
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bioRxiv - Genomics 2020Quote: ... Salt or Proteinase K treated samples were purified using 1.8X volumes of AMPure XP (Beckman Coulter) beads prior to PCR amplification ...
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bioRxiv - Genomics 2020Quote: ... Salt or Proteinase K treated samples were purified using 1.8X volumes of AMPure XP (Beckman Coulter) beads prior to PCR amplification ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was centrifuged at 10,500 rpm (∼20,000 × g, k factor = 1,753) for 70 min (SW28 rotor, Beckman). Pelleted lEVs were resuspended in 13.5 ml of PBS (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... and sedimented again at 10,500 rpm (∼20,000 × g, k factor = 1,983) for 70 min (SW40 rotor, Beckman). The supernatant containing sEVs was centrifuged at 25,000 rpm (∼ 110,000 × g ...
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bioRxiv - Cell Biology 2023Quote: ... Centrifugations at 10,000 g (k-factor = 2547.2) and 100,000 g (k-factor = 254.7) were done at +4°C using polyallomer tubes (Beckman Coulter Inc. ...
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bioRxiv - Microbiology 2024Quote: ... at 28 K rpm for 2 h at 4°C in an SW32 swing-bucket rotor (Beckman). SG3ΔEnv virions were thoroughly resuspended using 500 μl Hepes/Salt buffer per tube ...
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bioRxiv - Cell Biology 2021Quote: ... 100K EVs were isolated by ultracentrifugation of 10,000xg supernatant at 100,000xg for 65 min in a SW40 rotor (k-factor 280.3) (Beckman-Coulter). EV-containing 100K ultracentrifugation pellets were resuspended in 20 μL PBS containing 0.2% BSA (cleared from aggregates by ultracentrifugation for 16-20 hrs at 100,000xg) ...
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bioRxiv - Microbiology 2024Quote: ... (The filtrate was subjected to ultracentrifugation at 100,000g for 2 h (Optima L-90 K ultracentrifuge; Beckman Coulter). The supernatant was discarded and the pellet was washed with sterile PBS and filtered through a sterile 0.2 μm-pore polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2024Quote: ... Following an 18-hr centrifugation at 4°C (44,000 rpm, SW55 rotor, Beckman Optima LE-80 K Ultracentrifuge), 420-μl fractions (1-11 ...
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bioRxiv - Microbiology 2024Quote: ... which employs enzymatic digestion followed by proteinase K digestion and AMPure XP (Beckman Coulter, Inc. Brea, CA, USA) bead purification ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in p24 primary antibody solution (1:100 dilution of p24-FITC antibody clone KC57 (Beckman Coulter) diluted in perm/wash buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and the pellet was resuspended in 25 ∝l lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and Red-C12 fluorescence of the starting material ...
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bioRxiv - Bioengineering 2024Quote: ... cell pellets were treated with lysis buffer mix containing Proteinase K sourced from the RNAdvance Tissue kit (Beckman Coulter). Cells were mixed by vortexing ...
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bioRxiv - Immunology 2021Quote: ... Antibodies against the following molecules were included: CD49d (HP2/1; Beckman Coulter), CD32 (FUN-2 ...
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bioRxiv - Immunology 2023Quote: ... and intracellularly stained with the Fluorescein isothiocyanate-labeled KC57 (anti-HIV-1 p24) antibody (1:200) (Beckman Coulter) or a mixture of both antibodies ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with 1% FBS in PBS antibody staining buffer and stained with the following antibodies: CD64 PE-Cy7 (Cat. # B06025, Beckman Coulter); CD45 PerCP (Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... columns were spun at 100.000 g for 24 hours using a SW-28.1 rotor (k-factor: 276) in an optima XPN-80 ultracentrifuge (Beckman coulter), and subsequently fractions of 1 mL were collected ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 300,000×g at 4°C for 3 h in a TLA-110 fixed angle rotor (k-factor 13) (Beckman Coulter). The pellet was resuspended in 10 µL PBS of which 3 µL was adsorbed onto glow-discharged 2 nm carbon layer coated Quantifoil R1.2/1.3 400 mesh gold grids ...
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bioRxiv - Systems Biology 2023Quote: ... The urine was centrifuged at 20,000 x g for 30 minutes at 4°C (k-factor 1,790) in an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW32 Ti swinging bucket rotor (Rmin 67 ...
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bioRxiv - Microbiology 2020Quote: ... and p24 antibodies (anti-HIV core antigen: clone KC57, Beckman Coulter, 1:200).
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bioRxiv - Cell Biology 2020Quote: ... The 1 ml concentrates were overlaid on top of the gradient, and centrifuged at 100 000 g (28 000 rpm, k-factor 266) in an Optima L-90K ultracentrifuge (Beckman Coulter) using an SW41 Ti rotor for 18 h at 4 °C with slow deacceleration ...
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bioRxiv - Developmental Biology 2022Quote: ... proteinase K and heat for de-crosslinking (overnight at 65°C) followed by ethanol precipitation and SPRI bead clean up (Beckman Coulter). The resulting DNA was quantified by Clariostar (BMG Labtech) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were ultracentrifuged at 180,000 × g (38,000 rpm) in a SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter, k-factor 145) for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Crosslinks were reversed with 25 µg Proteinase K for 16 hours at 65°C and DNA was purified using AmpureXP beads (Beckman Coulter). Enrichment was confirmed using qRT-PCR with known positive and negative binding sites for each protein target ...
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bioRxiv - Biophysics 2022Quote: ... EVs were separated from protein aggregates and free reagents by bottom-up density gradient centrifugation in sucrose for 17.30 h at 192,000 g and 4°C using a SW40 rotor (k-factor 144.5; Beckman Coulter, Fullerton, California, USA). Twelve fractions of 1 mL were then collected from the top of the gradient and respective densities were determined by refractometry using an Atago Illuminator (Japan) ...
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bioRxiv - Systems Biology 2024Quote: ... the DNA bound to chromatin was recovered by proteinase K treatment and crosslink reversal and purified using SPRIselect beads (Beckman Coulter). The NEB NEBNext Ultra II DNA Library Prep Kit was used for Illumina sequencing ...
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bioRxiv - Microbiology 2021Quote: Antibodies used for flow cytometry: anti-HIV-1 p24-PE (KC57-RD1 Beckman Coulter); anti-HIV-1 Env clone PGT151 (gift from Laura McCoy ...
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bioRxiv - Immunology 2023Quote: Koff rates were determined as dissociation of reversible pMHC-Streptamers upon addition of D-Biotin in a flow cytometry-based assay (CyAn ADP Lx 9 color flow cytometer, Beckman Coulter) as described previously (44) ...
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bioRxiv - Biophysics 2022Quote: ... The tubes were filled with PBS to reach the final volume and samples were ultracentrifuged at 120,000 g for 60 minutes at 4°C (70.1 Ti rotor, k-factor 36, Beckman Coulter, Brea, CA, USA). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... Virus was concentrated from cell-free culture supernatant by high-speed ultracentrifugation through a 30% sucrose cushion at 80,000xg for 16 hrs in a SW32 rotor (k-factor 321) (Beckman Coulter, Brea, CA).
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bioRxiv - Cell Biology 2024Quote: ... while the supernatant was further centrifuged at 118.000 × g (38,800 rpm) (Type Ti 45 fixed-angle rotor, Beckman Coulter, k-factor 178) for 2.5 hours at 4°C to isolate small EVs that were resuspended in PBS and stored at –80°C.
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bioRxiv - Neuroscience 2023Quote: ... the samples were concentrated to a final volume of 240 µl through ultracentrifugation at 42,300 rpm using a Beckman TLA-55 rotor (RCFavg: 80,160; RCFmax: 110,220; k-factor: 110 and the Optima MAX-XP ultracentrifuge (both Beckman Coulter, Brea, CA, USA) and equal sample volumes were immediately used for the EV Neuro panel A and B (115±25 µl ...