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Citations for Beckman :
51 - 100 of 761 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The culture growth was counted every two days using a Coulter Counter (Beckman Coulter) for twelve days ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the two amplifications obtained (one per set) were purified with AMPure XP beads (Beckman-Coulter) at a 1:1 ratio according to the manufacturer’s recommendations and pooled at equimolar concentrations ...
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bioRxiv - Molecular Biology 2023Quote: ... and ∼450 bp DNA fragments were selected using two-sided AMPure XP bead (Beckman Coulter) size selection ...
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bioRxiv - Immunology 2023Quote: ... Next the pooled products underwent two sequential purification steps using AMPure XP beads (Beckman Coulter) with 0.6x and 0.8x volumetric ratio according to the manufacture’s instruction ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were pooled and purified with two rounds of AMPure XP beads (Beckman Coulter). The size and purity of the pooled library were assessed using a BioAnalyzer High Sensitivity DNA chip (Agilent technologies) ...
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bioRxiv - Immunology 2022Quote: ... purified with two rounds of 0.6X solid-phase reversible immobilization beads (AmpureXP, Beckman, or CleanNGS, Proteigene) and finally eluted in nuclease free water ...
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bioRxiv - Cancer Biology 2022Quote: ... at 22,000 rpm at 4°C for two hours in an Optima XE 90 (Beckman Coulter). Lentiviral pellets were resuspended in the minimal residual media present after aspirating off supernatant ...
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bioRxiv - Synthetic Biology 2020Quote: ... Two biological replicates of flow cytometry measurements were performed using a Gallios flow cytometer (Beckman Coulter), set to detect either 10000 or 100000 events per run ...
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bioRxiv - Neuroscience 2024Quote: ... and size-selected using two rounds (0.35x and 0.75x) of Agencourt AMPure XP beads (Beckman Coulter), as recommended by the Nextera XT protocol (Illumina) ...
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bioRxiv - Bioengineering 2023Quote: ... It is equipped with two liquid handlers (Biomek NX MC and Biomek NXp – Span8) (Beckman Coulter), one SCARA robotic arm ...
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bioRxiv - Pathology 2023Quote: ... Each subpool of PCR products was purified with two rounds of AMPure XP beads (Beckman Coulter) at 1.2× sample volume of beads.
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bioRxiv - Biochemistry 2023Quote: ... using a two-channel centerpiece equipped with an An-50 Ti rotor (Beckman Coulter Inc., USA). Each sample was diluted to achieve a final volume of 400 μL (with an A280 nm absorption of approximately 1.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... After two short clearance spins (Type 45 Ti rotor from Beckman Coulter, 15 min at 20000 rpm), lysates were spun for 24 h at 4°C at 50000 rpm in a Type 70 Ti rotor (Beckman Coulter ...
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bioRxiv - Plant Biology 2019Quote: ... Primer dimers were subsequently removed using two rounds of purification using AMPure beads (Beckman Coulter, Indianapolis, IN). Quality and quantity of this amplified library was assessed as above ...
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bioRxiv - Developmental Biology 2022Quote: ... Magnetic bead purification with two-sided size selection using undiluted Agencourt AMPure XP Beads (Beckman Coulter, A63881) ensured library sizes between 150 and 1000 bp ...
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bioRxiv - Cancer Biology 2021Quote: ... Resulting libraries were subjected to a two-sided size clean up using SPRI beads (Beckman Coulter, Brea, CA) to obtain sizes between 200-1000bp ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was centrifuged at ∼100,000×g (28,000 RPM) for 1.5 h using two SW-28 rotors (Beckman Coulter). The pellet was resuspended with 200 µl phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2021Quote: ... DNA fragments were then selected for size (200-300bp) with two subsequent rounds of AMPure XP bead (Beckman) treatment and for the presence of the Biotin marker by Dynabeads MyOne Streptavidin C1 beads (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... and small-sized EVs were isolated in two steps by ultracentrifugation using a 70Ti rotor (Beckman Coulter, USA). First ...
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bioRxiv - Bioengineering 2020Quote: ... whole transcriptome products were isolated through two rounds of SPRI purification using Ampure Spri beads (Beckman Coulter, Inc.) at both 0.6X and 0.8x volumetric ratio and quantified using a Qubit.
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bioRxiv - Biochemistry 2020Quote: ... Then the ribosomes were pelleted through a sucrose cushion (1.1 M sucrose in HEPES:Polymix buffer with 15 mM Mg2+) at 50,000 rpm for two hours (TLS-55, Beckman), the pellet was dissolved in HEPES:Polymix buffer (5 mM Mg(OAc)2) ...
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bioRxiv - Cancer Biology 2020Quote: ... Resulting libraries were subjected to a two-sided size clean up using SPRI beads (Beckman Coulter, Brea, CA) to obtain sizes between 200-1000bp ...
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bioRxiv - Microbiology 2019Quote: ... and after that libraries were purified according to the two-sided AMPure XP bead purification protocol (Beckman Coulter). Profiles of the libraries were quantified on a BioAnalyzer High Sensitivity DNA chip ...
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bioRxiv - Immunology 2022Quote: ... Libraries were cleaned-up with two rounds of size selection with AMPure XP beads (24μl/12μl; Beckman Coulter) and eluted in 15μl of molecular grade water ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested by two rounds of centrifugation (6,000 rpm, 10 min, 4°C, JLA-9.1000 rotor; Beckman), resuspended in Buffer A containing 50 mM Tris-HCl pH8.0 ...
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bioRxiv - Genomics 2024Quote: ... the products of the two amplifications (one per primer set) were purified with AMPure XP beads (Beckman Coulter) at a 1:1 ratio according to the manufacturer’s recommendations and pooled at equimolar concentrations ...
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bioRxiv - Genomics 2024Quote: ... Libraries were purified by two rounds of double-sided size selection with AMPure XP beads (Beckman Coulter, A63881), with the initial round of 0.5× sample volume of beads followed by a second round with 1.3× initial volume of beads ...
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bioRxiv - Biochemistry 2021Quote: ... The library was cleaned up and size-selected by two consecutive rounds of binding to 1.2x and 0.8x SPRI beads (Agentcourt AMPure XP beads, Beckman Coulter). The integrity and size distribution of the final libraries (two biological replicates of KCl-treated and one of untreated ...
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bioRxiv - Microbiology 2020Quote: ... We removed degraded concatemers with two consecutive 0.8X SPRI purifications using Ampure XP beads (Beckman Coulter, Brea, CA, USA), eluted ...
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bioRxiv - Biochemistry 2019Quote: ... The cell lysates were subjected to two rounds of centrifugation at 18000 rpm for 5 and 30 mins in a JA-25.50 rotor (Beckman). Bradford assay was used to normalize protein levels for co-immunoprecipitation and immunoblot analysis ...
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bioRxiv - Microbiology 2019Quote: ... sucrose cushion in PBS 16,000 RPM for two hours at 4°C in a SW 28 swinging-bucket rotor (Beckman). Virus pellets were resuspended in PBS ...
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bioRxiv - Microbiology 2023Quote: 54 µL of media was dispensed into wells (in two 27 µL steps) using a BioMek FX (Beckman-Coulter). Drugs ...
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bioRxiv - Developmental Biology 2024Quote: ... A two-size selection of fragments was performed using 0.5x and 1.3x volume of AMPure XP beads (Beckman Coulter). Libraries were quantified and analyzed using Qubit and Tapestation assays ...
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bioRxiv - Systems Biology 2023Quote: ... Two libraries were prepared and sequenced: one with size-based selection for mRNA >4 kb using AMPure beads (Beckman) and a second library with no size selection.
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bioRxiv - Biochemistry 2023Quote: ... Density gradient centrifugation was done at 50,000 rpm for two hours at 4 °C using a 70.1 Ti rotor (Beckman Coulter). Post centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... and loaded onto a two-step CsCl step gradient 41 in a thick wall ultracentrifuge tube (Beckman Cat# 360743) Samples were loaded onto an ultracentrifuge (Sw32Ti rotor ...
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bioRxiv - Microbiology 2023Quote: The PCR products were purified using a two-sided SPRI beads cleanup at bead ratios of 0.5X and 0.9X (AMPure XP, Beckman Coulter), and their concentration was measured using high sensitivity D1000 DNA tapestation (Agilent).
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates were subjected to two rounds of centrifugation at 18000 rpm for 5 and 30 mins in a JA-25.50 rotor (Beckman). Protein levels were normalized for coimmunoprecipitation and immunoblot analysis using a Bradford Assay ...
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bioRxiv - Cell Biology 2020Quote: ... MOFLO system (Beckman Coulter) was used for the sorting at Wellcome-MRC Stem Cell Institute Flow Cytometry Facility.
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bioRxiv - Genetics 2022Quote: ... The concentration levels in serum were obtained by chemiluminescent immunoassay by Beckman Coulter Immunoassay Systems (Beckman Coulter ...
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bioRxiv - Cell Biology 2021Quote: ... MOFLO system (Beckman Coulter) was used for the sorting at Cambridge NIHR BRC Cell Phenotyping Hub.
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bioRxiv - Cell Biology 2021Quote: ... MOFLO system (Beckman Coulter) or Flexible BD InfluxTM cell sorter were used for the sorting at Cambridge NIHR BRC Cell Phenotyping Hub and data were analysed with FlowJo software (Tree Star).
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bioRxiv - Genomics 2020Quote: ... according to the manufacturer’s protocol with 12 cycles of PCR followed by two rounds of clean up with 1.3X Agencourt AMPure XP beads (Beckman Coulter). The resulting double-stranded DNAs were fragmented by Covaris sonicator (S220 ...
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bioRxiv - Cell Biology 2021Quote: ... 3 minutes on ice between sonication cycles) and loaded into a JE 5.0 elutriation rotor fitted for a two-chamber run (Beckman Coulter) in a J6-MI Centrifuge (2.4krpm ...
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bioRxiv - Neuroscience 2020Quote: ... sgRNA amplification was performed using a two-step PCR strategy and purified using Agencourt AMPure XP (A63880; Beckman Coulter UK), Illumina sequencing (19-bp single-end sequencing with custom primers on the HiSeq2500 Rapid Run ...
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bioRxiv - Developmental Biology 2020Quote: ... following the manufacturer’s instructions except the gel purification step was replaced with a two-sided AMPure XP bead (Beckman Coulter) size selection to obtain libraries between 200-500 bp ...
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Heatrich-BS enables efficient CpG enrichment and highly scalable cell-free DNA methylation profilingbioRxiv - Genomics 2021Quote: ... the sample was incubated at 25°C for 1 hour and then cleaned up by performing two rounds of 1.2x SPRI Select (Beckman Coulter). The sample was then subject to bisulfite conversion following the recommended protocol of Zymo EZ DNA Methylation kit (Zymo Research) ...
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bioRxiv - Immunology 2021Quote: ... Ultracentrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for two hours at 4°C using a Beckman SW28 rotor (Beckman Coulter) was performed to pellet the viruses through the sucrose cushion while soluble egg proteins were removed ...
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bioRxiv - Genetics 2020Quote: ... After two washes with FACS buffer cells were re-suspended on FACS buffer and assessed on CytoFLEX flow cytometer (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 minutes between sonication cycles) and loaded into a JE 5.0 elutriation rotor fitted for a two-chamber run (Beckman Coulter) in a J6-MI Centrifuge (2.4 krpm ...