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Citations for Beckman :
201 - 250 of 737 citations for Tumor Protein Translationally Controlled 1 TPT1 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... CD41 PE-Cy5 (1:200, Beckman Coulter) and GlyA PE (1:200 ...
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bioRxiv - Immunology 2021Quote: ... CD103 (clone HML-1, Beckman Coulter, IM1856U). Cells were stained at antibody dilutions of 1:30 ...
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bioRxiv - Immunology 2021Quote: ... supplemented with protease inhibitor cocktail and 1 mM TCEP and centrifuged at 40,000 RPM for 1 h (45 Ti fixed-angle rotor, Beckman). Supernatant was incubated with amylose beads pre-equilibrated in the same buffer for 1 h at 4 °C and loaded onto a gravity flow column ...
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bioRxiv - Biochemistry 2019Quote: ... 15 mM imidazole and lysed by sonication in the presence of 10 μL of 1 U.ml-1 DNase I per litre of over-expression culture and cell lysate was clarified (Beckman JA25.50 rotor ...
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bioRxiv - Biochemistry 2021Quote: ... 1□mM EDTA) and centrifuged at 25000 rpm for 1 hour at 4°C (Beckman Coulter SW41-Ti rotor). Mitochondria forming a band at the interphase between the 1 M and 1.5 M sucrose were collected and resuspended in 10 mM Tris-HCl pH = 7.5 in 1:1 ratio ...
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bioRxiv - Synthetic Biology 2020Quote: ... Reverse transcription reactions were then purified using Agencourt Ampure XP beads at a 1:1 dilution (Beckman-Coulter, A63881). Some portion of the eluent ...
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bioRxiv - Cell Biology 2019Quote: ... and size-selected twice with Agencourt AMPure XP (1:1.5 and 1:0.5 sample to beads; Beckman Coulter, USA). Libraries were quantified by KAPA Library Quantification Kit for Illumina Sequencing Platforms (KAPA Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA from each reaction was purified using a 1:1 ratio of AMPure XP beads (Beckman Coulter, USA). 5ml cDNA was amplified with Q5 High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Amplified PCR product was purified using a 1:1 concentration of AMPure XP beads (Beckman Coulter, Brea, CA, USA) and eluted in 30 μL of water ...
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bioRxiv - Molecular Biology 2021Quote: ... as determined by flow-cytometry with anti-CD34 antibody (#581-PE; Beckman Coulter, measured on Beckman Coulter FC500). CD34+ cells were seeded at a density of 50.000 cells/well in a 12 well plate in CellgroSGM (CellGenix ...
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bioRxiv - Genomics 2019Quote: ... Library purification and removal of adapter dimers was performed with Agencourt AMPure XP beads in a 1:1 library:beads ratio (Beckman Coulter). ChIP-seq libraries were sequenced for 75 bp single-end reads using the NextSeq 500/550 High Output v2 kit (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... Stained cells and fluorescence minus one controls were then resuspended in SB + 1:1000 DNase + 1:1000 Draq7 (Beckman Coulter) as a live/dead stain ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were purified and adapter dimers removed with Agencourt AMPure XP beads purification using a 1:1 library:beads ratio (Beckman Coulter). ChIP-seq libraries were sequenced on the Illumina NextSeq 500 system with a 20% phiX spike-in (Illumina ...
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bioRxiv - Immunology 2023Quote: ... the PCR plate was briefly centrifuged and 25 µl of room-temperature AMPure XP beads (1:1 ratio; Beckman Coulter) was added to each well and incubated for 8 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... The ratio of Ca2+-bound Indo-1 versus Ca2+-free Indo-1 was analyzed using FlowJo 9 software (Beckman Coulter).
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bioRxiv - Immunology 2022Quote: ... and anti-HIV-1 p24 PE (Beckman coulter) were used ...
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bioRxiv - Genetics 2019Quote: ... CD41 PE-Cy5 (1:200, Beckman Coulter, 6607116), GlyA PE (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... in 1 ml polycarbonate ultracentrifuge tubes (Beckman Coulter) and centrifuged at 250,000 g for 1 hour at room temperature with a TLA 120.2 rotor using an Optima XP benchtop ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Immunology 2021Quote: ... CD3-PB (1:20; clone UCHT1; Beckman Coulter), CD4-PE-Cy7 (1:100 ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were 1:1.3 AMPure XP (Beckman Coulter) purified and sent for sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... PE or FITC (J33, Beckman Coulter; 1:10) or CD3-APC ...
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bioRxiv - Cancer Biology 2024Quote: ... PE or FITC (UCHT1; Beckman Coulter; 1:10) were used to stain T-cells in co-culture assays ...
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bioRxiv - Microbiology 2019Quote: ... Intracellular Gag staining was carried out using a Gag monoclonal antibody conjugated to Phycoerythrin (KC-57 RD1 Beckman Coulter). 1×105 cells from a HIV GPV-zip coded library were washed once with FACS buffer and fixed with 100 µl of BD cytofix for 10 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2022Quote: ... The cells were stained with a panel of monoclonal antibodies containing: ECD CD16 clone 3G8 (Beckman Coulter; cat.no. A33098), BV711 CD45 clone HI30 (BD Biosciences ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... and then stained for flow cytometry using the CD34-phycoerythrin (PE) antibody (PE-pool, Beckman Coulter, IM1459U, Brea, CA). RNA-sequencing libraries were then prepared from RNA isolated from the bulk mutant AML bone marrow cells and the normal CD34+ bone marrow cells using TruSeq Stranded Total RNA kit (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... unbound PKH67 dye and unbound antibodies by overnight bottom-up sucrose density gradient (SDG) ultracentrifugation at 192,000 g (SW40 rotor Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: The TCR V beta repertoire kit contained antibodies to over 76% of V βeta families (Beckman Coulter, Fullerton, CA). The cells were stained with these antibodies in combination with tetramers or dextramers for 20 minutes at RT to determine the TRBV repertoire of antigen specific cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Platelet concentration was obtained by flow cytometry using an antibody against human CD41 labelled with PC7 (6607115, Beckman Coulter) and MP-count beads (6607115 ...
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bioRxiv - Genomics 2020Quote: ... followed by two size-selection steps with SPRIselect (1:0.6 and 1:0.2 sample vol. to beads vol.; Beckman Coulter, CA, USA). The libraries’ size distribution was determined by Bioanalyzer (Agilent Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... containing 10% FBS and 1% PS and 50 nL of FDA-approved compounds (final concentration: 1 μM) using a pin tool (Biomek FX, Beckman Coulter). Cells were added to each well (1,500 cells/25 μL ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were purified and adapter dimers removed with Agencourt AMPure XP beads purification using a 1:1 library:beads ratio (Beckman Coulter, #A63880). ChIP-seq libraries were sequenced on the Illumina NextSeq 500 system with a 20% phiX spike-in (Illumina ...
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bioRxiv - Genomics 2023Quote: ... and paramagnetic beads (1:1 beads/sample) using the same purification protocol as with AMPure XP beads (Beckman Coulter, no. B37419AB). The product purity was assessed via capillary electrophoresis on a QIAxcel instrument (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... The product of the reverse transcription reaction was purified with AMPure XP beads at a 1:1 bead to sample ratio (Beckman Coulter). Purified cDNA was split in half to amplify the heavy and light chains in separate reactions ...
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bioRxiv - Biochemistry 2021Quote: ... cells were washed twice with PBS/1% BSA and resuspended in 500 µl PBS/1% BSA for analysis on a CytoFLEX S flow cytometer (Beckman Coulter, Australia). FITC was excited using a 488 nm laser and emission read at 525 nm using a 525/40 band-pass filter with a gain setting of 300 ...
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bioRxiv - Neuroscience 2020Quote: ... Amplified cDNA samples were then cleaned up of short fragments with AMPure XP beads (1:1 ratio; Beckman Coulter, Brea, CA, USA) and checked for cDNA quality and concentration on a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Immunology 2020Quote: ... Plate was then incubated 1 h à 37 °C with 1 μg per well of alkaline phosphatase-coupled anti-Igκ or anti-IgM (Beckman Coulter) in PBS ...
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bioRxiv - Genomics 2021Quote: ... We further purified the materials eluted from the columns by adding 150 ul (1:1) of AMPure beads RNAclean XP (Beckman coulter, A63987) and incubating for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... The barcoded samples were pooled and purified using AMPure XP beads at a 1:1 ratio of sample:beads (Beckman Coulter; Brea, CA). The final libraries (350 – 800 ng DNA ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was examined on gel electrophoresis and cleaned up by the AMpure XP beads (PCR product: bead 1:1 v:v, Beckman Coulter, Krefeld, Germany). The generated libraries were pooled with equal molar DNA ...
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bioRxiv - Cancer Biology 2020Quote: ... isotype controls for intracellular stains and subjected to post-acquisition compensation using VersaComp Antibody Capture Bead Kit (Beckman Coulter #B22804). Data was analyzed using Kaluza Software (Beckman Coulter) ...
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bioRxiv - Immunology 2021Quote: ... as assessed by flow cytometry with Korman orange-conjugated mouse anti-human CD19 monoclonal antibody (mAb) (Beckman Coulter, CA, USA). The purified CD19+ B cells were cultured in the chemical-defined and serum-free X’VIVO 15 medium (Lonza ...
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bioRxiv - Immunology 2022Quote: ... Transfectants were stained for viability using a Live dead blue dye followed by anti-HLA DR DP DQ antibodies (BioRad clone Bu26, Cat. #MCA2497F; Beckman clone I3 ...
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bioRxiv - Immunology 2023Quote: Human TCR Vβ repertoire analysis was performed using antibodies against the most frequent TCR Vβ variants (covering about 70% normal human TCR Vβ repertoire, as reported by Beckman Coulter “Beta Mark TCR Vbeta Repertoire Kit” (cat.nr. ...
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bioRxiv - Genetics 2019Quote: ... and GlyA PE (1:200, Beckman Coulter, IM2211U, KC16). Finally ...
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bioRxiv - Genetics 2019Quote: ... CD41 PE-Cy5 (1:200, Beckman Coulter, 6607116, P2) and GlyA PE (1:200 ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... CD45-PC7 Ab (clone J33, 1/15, Beckman Coulter), CD3-BV510 Ab (clone HIT3a ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 mM DTT) in a TLA100.3 rotor (Beckman) at 116,000 xg for 5 h ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... erythrocytes were lysed (1 ml of Versalyse, Beckman Coulter) and the lymphocytes fixed (25 μl of Fixative Solution ...
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bioRxiv - Immunology 2020Quote: ... APC-A750 anti-CD3 (Beckman Coulter, clone UCHT-1), APC anti-CD4 (Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mM EDTA in centrifuge tubes (#344060, Beckman Coulter). The samples were spun in an ultracentrifuge (rotor ...