Labshake search
Citations for Beckman :
251 - 300 of 1320 citations for Tick Borne Encephalitis Virus Lysate Strain Moscow B 4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifuging at 14,000 rpm for 15 minutes (Beckman Coulter JA-20 rotor). Imidazole was added to the supernatant to final concentration of 10mM loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
-
bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified by centrifugation at 35 Krpm for 30 min in a 45ti rotor (Beckman). The supernatant was filtered (0.45 um ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation at 56,000 rpm in a Type 70 Ti rotor (Beckman Coulter) at 4°C for 1 hour ...
-
bioRxiv - Biophysics 2023Quote: ... lysate was centrifuged at 20000 rpm for 20 minutes with a Ti-45 rotor (Beckman Coulter, USA) to remove the cell debris ...
-
bioRxiv - Molecular Biology 2024Quote: ... Macromolecular complexes were separated by ultracentrifugation of the lysates in a swinging-bucket rotor (Beckman SV40 Ti) for 16 h at 285,000 x g ...
-
bioRxiv - Neuroscience 2021Quote: ... KG, Staufen, Germany) (position 6, 4 × 5-s bursts) and subsequently centrifuged at 48,000g at 4°C (Beckman Avanti J-251 Ultracentrifuge ...
-
bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
-
bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
-
bioRxiv - Microbiology 2020Quote: ... 4°C in an SW55Ti rotor (Beckman), and the virus pellet harvested for analysis by WB with αGag.
-
bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
-
bioRxiv - Cell Biology 2020Quote: ... 4 °C with 70Ti rotor (Beckman Coulter) to collect sEV pellets in double 0.22 μm-filtered PBS ...
-
bioRxiv - Microbiology 2022Quote: ... 4 °C in SW41 rotor (Beckman Coulter). Samples were fractionated and collected using Biocomp Piston Gradient Fractionator (BioComp Instruments) ...
-
bioRxiv - Microbiology 2022Quote: ... 4 °C in SW28 rotor (Beckman Coulter). Gradients were fractionated using Biocomp Piston Gradient Fractionator (BioComp Instruments) ...
-
bioRxiv - Microbiology 2024Quote: ... 4 °C in SW41 rotor (Beckman Coulter). Polysomes profiles were obtained using Biocomp Piston Gradient Fractionator (BioComp Instruments ...
-
bioRxiv - Microbiology 2024Quote: ... 4°C (Optima TLX Ultracentrifuge, Beckman Coulter). The pellet was resuspended in 200 µL of ultrapure water.
-
bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared by centrifugation (60 minutes, 40,000 RPM) in a Type 45 Ti rotor (Beckman Coulter), filtered and loaded onto a HisTrap HP column (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Lysate was clarified by centrifugation for 30 min at 18000 rpm in a JA-20 rotor (Beckman-Coulter). The supernatant was layered over a sucrose cushion of 24mL buffer B (buffer A with 500mM NH4Cl ...
-
bioRxiv - Cell Biology 2021Quote: ... The soluble lysate fraction was collected after centrifugation for 1 hour at 16000 rpm (Beckman Coulter JA-25.50) at 4°C ...
-
bioRxiv - Neuroscience 2022Quote: Microglia were isolated from whole brain cell lysate using the BC MoFlo Astrios flow cytometer (Beckman Coulter, USA) which was set up for operation according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... and the lysate was then centrifuged for 30 minutes at 45000 rpm in a Ti45 rotor (Beckman Coulter). Supernatant was then incubated rotating with 1ml of amylose resin (NEB ...
-
bioRxiv - Biophysics 2019Quote: ... and the lysate was clarified by centrifugation for 1 h at 192,838 g in a Type 50.2 Ti rotor (Beckman) at 4°C ...
-
bioRxiv - Biophysics 2022Quote: ... Cells were lysed by sonication and the lysate was clarified by centrifugation at 18,000 rpm (25,000 xg) for 30 minutes (JA-20 rotor, Beckman). Supernatant was then applied to a 1 mL HisTrap column (Cytiva) ...
-
bioRxiv - Biochemistry 2023Quote: ... Cell lysate was clarified by centrifugation for 30 mins at 16,000 rpm using JLA-16.250 rotor (Beckman Coulter). The supernatant was kept and incubated with equilibrated IgG resin (Smart-Lifesciences ...
-
bioRxiv - Cell Biology 2021Quote: ... then finally at 100,000 g for 4 h at 4 ℃ using an SW28 rotor (Beckman Coulter, Fullerton, CA, USA). The pelleted exosomes were collected then subsequently resuspended in PBS ...
-
bioRxiv - Cell Biology 2021Quote: ... Hettich) at 4°C and then centrifuged at 40000 rpm for 4h30 at 4°C using SW41Ti rotor (Beckman). The supernatant was then UV inactivated at a wavelength of 254 nm for 4 min (3 times ...
-
bioRxiv - Microbiology 2023Quote: ... The lysates containing matured PsVs were loaded on an OptiPrep gradient that had been stabilized at least 1 h and centrifuged at 50k x g for 3.5-4 h at 4°C in a SW-55Ti rotor (Beckman). Fractions were collected in siliconized tubes and subjected to SDS-PAGE followed by Coomassie blue staining to assess the abundance of L1 and L2 proteins ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were spun at 200,000 × g at 4°C for 4 h in an MLS-50 rotor (Beckman Coulter), and 60 µL of top layer was collected ...
-
bioRxiv - Evolutionary Biology 2022Quote: Competition assays were performed by using GFP-tagged strains to distinguish between populations with flow cytometry (CytoFLEX Platform Beckman Coulter Life Sciences, USA). Parameters were ...
-
bioRxiv - Biophysics 2019Quote: ... Total volume was centrifuged at 10000 xg for 30 minutes at 4°C at 10000 xg for 30 minutes at 4 °C (Beckman Coulter Optima L-80 XP Ultracentrifuge ...
-
bioRxiv - Biochemistry 2020Quote: ... which were centrifuged at 50,000rpm for 4 hours at 4°C using a TLS55 rotor and an Optima MAX-XP Ultracentrifuge (Beckman Coulter). The sample was physically fractionated into 10 fractions of 85 uL each ...
-
bioRxiv - Molecular Biology 2022Quote: ... 800 µl of EDTA-treated lysate was layered on top of the sucrose gradient and ultracentrifuged at 35000 rpm for 4 hours at 4 °C in a SW41Ti rotor (Beckman). After centrifugation ...
-
bioRxiv - Immunology 2020Quote: ... all CCMV CP preparations were purified two times using an ultracentrifuge and 30% sucrose cushion – first with additional 0.5% Triton X-100 and the second time without Triton X-100 (4 h, 50 000 rmp, 4°C; Type 70Ti rotor, Beckman) and the pellet was then dissolved in buffer A ...
-
bioRxiv - Cell Biology 2022Quote: ... 4–40% sucrose gradient prepared in lysis buffer and centrifuged at 150,000 × g for 4 or 16 h at 4°C in a MLS-50 rotor (Beckman Coulter). Thereafter ...
-
bioRxiv - Neuroscience 2023Quote: ... the samples were filtered with a membrane pore size of 0.2 μm and centrifuged at 120,000 × g for 4 h at 4 °C in polycarbonate bottle assemblies (Beckman coulter, 355603) in a 70.1 Ti fixed angle rotor (Beckman coulter ...
-
bioRxiv - Immunology 2021Quote: ... Lysates were centrifuged at 200,000 xg for 90 min in an Optima XPN-80 ultracentrifuge (Beckman Coulter, IN, USA) and filtered supernatants were loaded on immunoaffinity columns ...
-
bioRxiv - Biochemistry 2019Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
-
bioRxiv - Biochemistry 2019Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 1.5 mL of anti-Flag M2 affinity gel (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2019Quote: ... The lysate was clarified by centrifuging at 183,960 x g for 88 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
-
bioRxiv - Biochemistry 2019Quote: ... 15 mM imidazole and lysed by sonication in the presence of 10 μL of 1 U.ml-1 DNase I per litre of over-expression culture and cell lysate was clarified (Beckman JA25.50 rotor ...
-
bioRxiv - Microbiology 2019Quote: ... was added into 20μl cell lysate and luciferase activity was measured using a DTX800 Multimode plate reader (Beckman Coulter). The remainder of the cell lysate was collected for western blots and GAPDH immunostaining ...
-
bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 183,960 x g for 88 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
-
bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 1.5 mL of anti-Flag M2 affinity gel (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Whole-cell lysates were spun at 35k rpm for 45 min in a Type 45 Ti rotor (Beckman Coulter), and the supernatant was decanted and filtered through a 0.45 μm cellulose acetate membrane ...
-
CM1-driven assembly and activation of Yeast γ-Tubulin Small Complex underlies microtubule nucleationbioRxiv - Biochemistry 2021Quote: ... Lysate was cleared by ultracentrifugation at 40,000 x g for 30 min in a Type 45Ti rotor (Beckman-Coulter). Xrcc4-Spc110164-207 was then purified by NiNTA affinity chromatography followed by addition of 3C protease overnight at 4° C to cleave the 6His tag ...
-
bioRxiv - Neuroscience 2019Quote: ... Then the lysates were spin down at 67,000 rpm for 1 hour at 18 °C with the ultracentrifuge (Beckman). The AAV particles were collected by aspirating the interface between 40-60% iodixanol with a syringe ...
-
bioRxiv - Cell Biology 2020Quote: ... After heating the lysate was allowed to cool and then centrifuged at 164,700 × g (Optima XPM ultracentrifuge, Beckman Coulter) for 45 min at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 183,960 x g for 88 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
-
bioRxiv - Genetics 2020Quote: ... 5 μL of each PCR reaction from the single worm lysate was pooled and purified by SPRI (Beckman Coutler) to remove unincorporated oligos ...
-
bioRxiv - Biophysics 2022Quote: ... Cells were lysed by sonication and the lysate was clarified by centrifugation at 18,000 rpm (25,000 xg JA-20 rotor, Beckman-Coulter) for 30 minutes ...