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Citations for Beckman :
501 - 550 of 646 citations for Testosterone D5 98% 100 Ug Ml In Dioxane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 500 μl M2 eluate was added to the top of a 12 ml 15 - 35 % glycerol gradient and centrifuged in an SW40Ti rotor (Beckman) at 40 ...
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bioRxiv - Cell Biology 2021Quote: ... coli cells obtained from 500 mL culture were harvested using centrifugation for 10 min at 4800 rpm (Beckman, Allegra-30XR), and were subjected to freezing in liquid nitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Phases were separated by centrifugation for 15 min at 4500 g and 6.5 ml of the aqueous phase was transferred to an ultracentrifugation tube (Ultra-Clear, open-top; Beckman coulter) filled with a 2 ml cushion of 5.7 M CsCl in 25 mM sodium citrate ...
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bioRxiv - Microbiology 2021Quote: 3 mL of pseudoparticles were pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter, Inc.) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... ‘Ex− medium’ was prepared by sedimenting 35 ml of ‘RPMI+’ medium supplemented with 20 % FBS by ultracentrifugation in a SW32 or SW28 swing-out rotor (Beckman) at 4 °C for at least 16 h ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants (3 ml) were loaded on the top of step gradients preformed in polyallomer tubes (14 by 89 mm, Beckman), containing 2 ml each of 1.82 ...
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bioRxiv - Cancer Biology 2021Quote: ... The EVs were washed once in 1 ml of PBS and purified by centrifugation at 100,000xg for 80 min in an Optima MAX-XP Ultracentrifuge (Beckman Coulter). EV protein concentration was measured using MicroBCA assay (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was then overlaid onto a 2 ml 60% iodixanol cushion and centrifuged at 100,000 × g (SW32 rotor, Beckman Coulter) for 18h ...
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bioRxiv - Biophysics 2022Quote: ... the homogenized solution was brought to 55 mL with more Percoll buffer and centrifuged at 48400 g for 30 minutes in a 45 Ti rotor (Beckman). Chromosomes appeared as a faint band about 1/5th from the bottom of the centrifuge tube ...
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bioRxiv - Molecular Biology 2022Quote: ... Stained cells were resuspended in sort buffer at 1 × 106/ml for fluorescence analysis on a Cytoflex S cell analyser (Beckman). SARS-CoV-2 Spike variants were generated by site-directed mutagenesis with Q5 polymerase (New England Biolabs ...
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bioRxiv - Biophysics 2022Quote: T1ST3SC12-(Ndc80)x modules were separated from Ndc80 on a 15-50% glycerol gradient of approximately 12 ml in a SW40 rotor (Beckman) for 16 hrs at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... were mixed 1:1 with liposomes (6 mg/ml) and floated for 2h at 55 000 rpm in a TLS-55 rotor (Beckman) at 25°C through a sucrose gradient ...
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bioRxiv - Neuroscience 2020Quote: ... The V and VI fractions were diluted to a total volume of 12 mL with dfPBS and centrifuged at 140,000 × g for 70 min at 4°C (# Optima-XE SW41 Beckman Coulter), and each pellet were resuspend with 30 μl of dfPBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The following day the viral pellet was resuspended in 5 ml PBS and centrifuged at 20,000 rpm for 90 mins at 4°C (Optima XL-100K, Beckman Coulter). The pellet was then resuspended in the required volume of PBS before being aliquoted and stored at −70°C.
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bioRxiv - Cancer Biology 2022Quote: ... Then 200nL of 5ug/mL of MHCI complex sample were dispensed from the Echo qualified 384- well source plate (Beckman Coulter Life Sciences ...
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bioRxiv - Immunology 2022Quote: ... Every 4 days cells were fed by replacing 25 μl of media with fresh I10 containing irradiated 2.5 × 103 3T3-msCD40L cells and 50 ng/mL IL-21 using a Biomek NXP liquid handler (Beckman Coulter). Cells were cultured for 2-3 weeks before harvesting the final supernatant and either freezing the cultures in the 384-well plate (50ul freezing media per well (FBS + 10% DMSO)) ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting homogenate was mixed with 6 mL sucrose (2 mol/L) and 2.5 mL CaCl2 (0.1 mmol/L) in an ultra-clear centrifuge tube (#344059, Beckman Coulter, USA). Then ...
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bioRxiv - Microbiology 2021Quote: ... 2 ml of each sucrose concentration was layered from 70% to 25% in open-top ultracentrifuge tubes (Beckman Coulter, 344059) immediately before use ...
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bioRxiv - Immunology 2021Quote: ... subsequently overlaid onto 5 mL of 20% sucrose and ultracentrifuged at 24,000 rpm for 2.5 hrs (Beckman XPN80, SW32.Ti). The viral pellet was resuspended in PBS.
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Proteome of the secondary plastid of Euglena gracilis reveals metabolic quirks and colourful historybioRxiv - Evolutionary Biology 2019Quote: ... The DNase-treated lysate (5 ml) was loaded on top of a sucrose discontinuous density gradient in ultracentrifuge tubes (Cat. No. 344058, Beckman). The gradient was prepared by layering decreasingly dense sucrose solutions upon one another from 2.0 ...
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bioRxiv - Biophysics 2019Quote: Sedimentation velocity data of the N-terminal RBCC domain of KAP1 (0.5 mg/mL) were obtained using a Beckman Optima XL-I analytical ultracentrifuge (Beckman Coulter) with an absorbance optical system ...
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bioRxiv - Molecular Biology 2021Quote: ... Sucrose solutions were made in Buffer G and 25 x 89 mm 38.5 mL open-up polyallomer tubes were used (Beckman Coutler). Ultra-centrifugation was performed (160,000 x g ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was transferred into precooled 250 mL centrifuge bottles and centrifuged at 20,000 rpm (Avanti® JE centrifuge and JLA-16.250 rotor from Beckman Coulter) for 15 min at 4°C.
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bioRxiv - Microbiology 2020Quote: ... through a 1 ml 30% sucrose cushion (in phosphate-buffered saline, PBS, pH 7.4) in a SW41 rotor (Beckman coulter). The pelleted virus material was re-solubilized in PBS and analyzed by sodium docecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Microbiology 2021Quote: ... 60% w/v) and centrifuged at 64,000 x g for 3 hours in A27-8×50 mL rotor (Beckman Coulter). The visible virus bands were collected ...
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bioRxiv - Immunology 2021Quote: ... the pellets were resuspended in cold PBS and transferred to one Ultra-Clear 13.2 mL tube (Beckman Coulter, ref 344059) and centrifuged again at 100,000g 4°C for 1 hour and 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter, USA) through a 20% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... buffer C containing 73% sucrose and buffer C containing 53% sucrose were layered in a 13 ml tube (Beckman Coulter), starting from the bottom ...
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bioRxiv - Cell Biology 2022Quote: ... 4–40% sucrose gradient prepared in lysis buffer and centrifuged at 150,000 × g for 4 or 16 h at 4°C in a MLS-50 rotor (Beckman Coulter). Thereafter ...
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bioRxiv - Cancer Biology 2022Quote: ... Fractions 2–9 were diluted 1:10 in PBS and centrifuged at 20,000 ×g (bEVs) or 100,000 ×g (sEVs) at 4°C for 1 h with an MLS-55 rotor (Beckman Coulter). The bEV and sEV pellets were suspended in double 0.22-µm-filtered PBS and were either used directly for experiments or stored in aliquots at −80°C until subsequent BLI ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was then overlaid onto a 2 ml 60% iodixanol cushion and spun at 100,000 x g (SW32 rotor, Beckman Coulter) for 18h ...
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bioRxiv - Biochemistry 2023Quote: ... Eight 50 μL PCR reactions were combined in a 1.5 mL Eppendorf tube and mixed with 320 μL Ampure XP beads (Beckman Coulter) (0.8:1 ratio ...
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bioRxiv - Cell Biology 2023Quote: ... down to 6 ml and ultracentrifuged at 10,000xg for 16 min at 4°C in MLA-80 rotor (Beckman Coulter). The pellet was resuspended and washed with PBS at the same conditions (volume ...
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bioRxiv - Biophysics 2023Quote: ... followed by layering the cleared lysate onto 40 mL of 2 M sucrose cushion and ultracentrifugation in Ti-45 rotor (Beckman) at 42,000 rpm for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... and pellet was resuspended and homogenised for final purification by sucrose cushion separation after ultracentrifugation in an MLS 50 rotor (Beckman). The final purified virus stock was diluted in TNE buffer (20 mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2023Quote: A 9-step discontinuous isosmotic iodixanol density gradient was prepared in a 13.2 mL open-top thinwall ultra-clear centrifugation tube (#344059, Beckman Coulter) using Optiprep and iodixanol buffer B (IBB ...
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bioRxiv - Microbiology 2023Quote: ... and 5.5 ml was layered on top of a two-step sucrose gradient consisting of 5 ml of 40% sucrose solution layered on top of 1.5 ml of a 65% sucrose solution in a 14 x 89mm Ultra-Clear tube (Beckman Coulter). All sucrose (w/w ...
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bioRxiv - Cell Biology 2024Quote: ... EVs were then placed in the bottom 36% layer of a 6-step gradient in a 13.2 ml open top polyallomer tube (331372, Beckman Coulter). A reference gradient was prepared in parallel and OptiPrep absorbance was measured at 340 nm to estimate the fraction density ...
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bioRxiv - Cell Biology 2022Quote: ... Yeast cells were stained with 5ug\ml propidium iodide for 10 minutes and samples were examined in CytoFlex flow cytometer (Beckman Coulter) using PE channel ...
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bioRxiv - Biophysics 2022Quote: ... in order to concentrate the medium to use the ultracentrifuge tubes with a reduced volume capacity for the small EV isolation (8.9 ml polypropylene centrifuge tube, Beckman Coulter, 361623). The tubes were filled with PBS to reach the final volume and samples were ultracentrifuged at 120,000 g for 60 minutes at 4°C (70.1 Ti rotor ...
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bioRxiv - Genomics 2020Quote: ... 300ul was transferred to a fresh 1.5-mL Eppendorf tube for two rounds of purification using AMPure XP paramagnetic beads (Beckman Coulter, Inc.) with ratios of 0.5X and 1.3X respectively ...
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bioRxiv - Systems Biology 2020Quote: ... and 1 mL of 5% (w/v) iodixanol solutions on top of each other in a 5 mL open top polyallomer tube (Beckman Coulter). Crude exosomes were resuspended in 200 µL PBS and overlaid on top of the gradient ...
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bioRxiv - Neuroscience 2019Quote: ... with 2 ml of a 20% sucrose in dPBS cushion and ultracentrifugated at 20,000 RPM (Beckman Coulter SW 32 Ti rotor) at 4°C for 2 hours ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were diluted 100-fold in PBS to reach the final cell concentration of approximately 106 cfu/mL and measured on a CytoFLEX S (Beckman Coulter) using a FITC fluorescence channel with a 488 nm excitation laser and 525/40 nm emission band-pass filter with side scatter triggered events threshold set to 10,000 events ...
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bioRxiv - Microbiology 2020Quote: ... Whole blood staining was done with Alexa Fluor®647 labelled-VLP (2.5µg/ml) and cell subsets were discriminated using the following antibody panel: CD45 (Clone J33; Beckman Coulter, Brea, CA), CD11c (Clone BU15 ...
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bioRxiv - Microbiology 2021Quote: ... triplicate spore preparations were made in 3 ml of 20% glycerol and suspended in Isoton II (Beckman Coulter, Brea, CA, USA). The particle volume was then measured for a minimum of 100 000 particles per sample using a Multisizer 4e Coulter counter (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... starting with 50% and topping off with 15% for a total of 11 mL was added to thin wall polypropylene ultracentrifuge tubes (Beckman Coulter). The tubes were immediately frozen and stored at -80 °C ...
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bioRxiv - Microbiology 2021Quote: ... the cell suspensions were diluted in 1 mL and loaded onto a cytoflex-LX flow cytometer (Beckman Coulter, Brea, CA, USA) with a 375 nm laser ...
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bioRxiv - Biophysics 2021Quote: ... and the fractions containing the protein of interest were combined and concentrated to 2mL at a final concentration of 3.85 mg/mL by repeated centrifugation in an Amicon Ultra Centrifugal Filter at 4k rpm for 5 min at 4 °C (Beckman SX4250). The concentrated protein was incubated overnight with TEV (1:50 mass ratio ...
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bioRxiv - Microbiology 2022Quote: A 3 ml volume of pseudoparticles was pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter) for 2 hours at 42,000 rpm at 4°C ...