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Citations for Beckman :
101 - 150 of 271 citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Phenotypic and genotypic adaptation of E. coli to thermal stress is contingent on genetic backgroundbioRxiv - Evolutionary Biology 2023Quote: ... and measuring cell density in volumetric mode on a Multisizer 3 Coulter Counter (Beckman Coulter). An isolate survived if its cell density measurements were maintained over the course of the test while allowing for fluctuations of +/− 1×106 cells.
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bioRxiv - Biophysics 2023Quote: ... precipitated by ultracentrifugation (35,000 rpm for 3 h at 4 ° C, Beckman-Coulter L90K, USA), and then redissolved in TNE/5 buffer.
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bioRxiv - Cell Biology 2020Quote: ... Samples are then resuspended in PBS and incubated with APC-A700 anti-human CD56 (N901, #B92446 Beckman Coulter), APC-A750 anti-human CD90 (Thy-1/310 ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentration of the expanding human T cells was calculated on a Coulter Multisizer (Beckman Coulter, Brea, CA) and maintained at 1.0-2.0 × 106 cells per mL in R10 media (RPMI-1640 plus GlutaMAX-1 ...
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bioRxiv - Immunology 2022Quote: ... GEM-RT products were subsequently amplified using human BCR primers and cleaned up through SPRIselect beads (Beckman Coulter). Next ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of Caco-2 cells and human ileum organoids was extracted using RNAdvance Tissue kit (Beckman Coulter) and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research) ...
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bioRxiv - Bioengineering 2023Quote: ... or by staining with anti-human TCR mAbs (Vβ13.1-IMMU 222, Beckman Coulter for A2/NY A97L-TCR). Cell staining was performed at 4°C for 30min.
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bioRxiv - Microbiology 2020Quote: ... 3′ ligated RNA was cleaned by incubating with 2.5X volume of RNAClean XP beads (Beckman Coulter) and 1.5X volume of isopropanol for 15 min ...
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bioRxiv - Neuroscience 2019Quote: ... and 3% Percoll (1 ml each) in Polycarbonate centrifuge tubes (13 x 51 mm; Beckman Coulter). The gradients were then centrifuged for 15 min at 18,700 rcf ...
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bioRxiv - Biophysics 2020Quote: ... After centrifugation at 30,000 rpm (4 ° C for 3 h) in the SW45Ti rotor (Beckman Instruments), eight 1.5-ml fractions were collected from the top of a gradient ...
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bioRxiv - Biochemistry 2020Quote: ... Gradients were resolved at 36,000 rpm for 3 hours at 4 °C in SW41 rotor (Beckman). Both separation and fractionation of gradients used a Biocomp Gradient Station (BioComp Instruments ...
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bioRxiv - Biochemistry 2020Quote: ... subjected to centrifugation (36,000 rpm for 3 hours at 4 °C, SW-41Ti Beckman Coulter rotor) and analysed using Biocomp Gradient Station (BioComp Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were counted using the electrical sensing zone method (Multisizer 3, Beckman Coulter, Indianapolis, IN)
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bioRxiv - Microbiology 2023Quote: ... 3′ ligated RNA was cleaned by incubating with 2.5X volume of RNAClean XP beads (Beckman Coulter) and 1.5X volume of isopropanol for 15 min before separation on a magnetic rack ...
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bioRxiv - Cell Biology 2023Quote: ... Cell density and sizing was measured with a Coulter counter (Beckman Coulter Multisizer 3, CA, USA). Raw cell diameter event counts were converted to relative frequency and visualized as ridgeline plots with the R tidyverse packages (https://tidyverse.tidyverse.org/ ...
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bioRxiv - Cancer Biology 2024Quote: ... Caspase 3 and 8 activity (EGFP+ cells) was analyzed using the CytoFLEX Flow Cytometer (Beckman Coulter) and Flowjo v10 BD (Becton Dickinson ...
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bioRxiv - Immunology 2022Quote: ... human Vγ9Vδ2 T lymphocytes were collected and stained with a FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and analyzed by flow cytometry ...
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bioRxiv - Cancer Biology 2024Quote: ... Platelet concentration was obtained by flow cytometry using an antibody against human CD41 labelled with PC7 (6607115, Beckman Coulter) and MP-count beads (6607115 ...
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bioRxiv - Immunology 2024Quote: Cells were washed in DPBS plus 0.5% human serum albumin and incubated with anti-CD45-KrO (#B36294, Beckman Coulter), anti-CD3-APC (#IM2467 ...
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bioRxiv - Cell Biology 2019Quote: ... and centrifuged at 200,000 g for 3 h at 4°C in a TLS55 rotor (Beckman Coulter). The sucrose gradient was fractionated manually from the top ...
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bioRxiv - Biochemistry 2020Quote: ... One was through twice daily measurement of 2 or 3 levels of QC specimens obtained from Beckman Coulter Inc ...
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bioRxiv - Cell Biology 2021Quote: The average cell density in a population was measured by comparing Multisizer 3 Coulter Counter (Beckman Coulter)-based cell volume measurements and suspended microchannel resonator (SMR)-based buoyant mass measurements ...
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bioRxiv - Plant Biology 2022Quote: ... The number of intact cells were measured using a Coulter Counter (Multisizer 3, Beckman Counter, Brea, CA).
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bioRxiv - Cancer Biology 2022Quote: ... the genomic DNA was cleaned up with AMPure XP beads at 3:1 ratio (Beckman Coulter A63880) and eluted in the 10 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were analyzed using a 3-laser Beckman Coulter Gallios using Kaluza Software (Beckman Coulter, Brea, CA).
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bioRxiv - Neuroscience 2020Quote: ... Samples were analyzed with a 3-laser Beckman Coulter Gallios using Kaluza Software (Beckman Coulter, Brea, CA).
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bioRxiv - Genetics 2020Quote: ... (3) a 1x post ligation bead cleanup with AMPure XP beads (Beckman Coulter Life Sciences, IN, USA) was performed and (4 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were washed 3x with PBS + 3% BSA and fluorescence was quantified using a CytoFLEX (Beckman Coulter) flow cytometer ...
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bioRxiv - Plant Biology 2022Quote: ... The gradients were ultra-centrifuged for 3 h at 175,000g in a SW41Ti rotor (Beckman-Coulter, USA) and analyzed by measuring continuous absorbance at 260 nm using a Piston Gradient Fractionator (Biocomp Instruments) ...
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bioRxiv - Molecular Biology 2022Quote: ... The gradients were centrifuged at 35,000 rpm for 3 hours at 4°C (SW41 Ti rotor; Beckman) and subsequently fractionated using a Piston Gradient Fractionator (Biocomp instruments ...
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bioRxiv - Cell Biology 2023Quote: ... The positive ratio of SSEA-3+ cells was analyzed by the CytoFLEX S Flow Cytometer (Beckman Coulter). Flow cytometry data were analyzed by Kaluza Analysis Software (Beckman Coulter).
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bioRxiv - Cancer Biology 2023Quote: ... Ca2+ dependent fluorescence intensity of Fluo-3/AM was measured by flow cytometry (CytoFLEX LX Beckman Coulter).
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bioRxiv - Biophysics 2023Quote: ... iVCL supernatant was centrifugated for 3 h at 25000 rpm and 4 °C (Beckman-Coulter L90K, USA). Precipitate was resuspended in the TNE buffer (0.1 M Tris-HCl ...
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bioRxiv - Bioengineering 2023Quote: ... Microbubble concentration and number- and volume-weighted size distributions were measured with a Multisizer 3 (Beckman Coulter). Microbubble concentration (cl ...
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bioRxiv - Cell Biology 2023Quote: Cell size was measured directly by a Coulter Counter (Multisizer 3; Beckman-Coulter, Miami, Florida, United States). Mitotic index assay was determined as described previously (Umen and Goodenough ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged for 3 h at 40 000 rpm in a SW41swing out rotor (Beckman Coulter). The separated gradient was then fractionated by upward displacement with 55% sucrose solution using a gradient fractionator (Brandel Inc. ...
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bioRxiv - Microbiology 2021Quote: ... the percentages of anti-human IgG postive cells in GFP positive cells were then analyzed using flow cytometer (Cytoflex S, Beckman). LIVE/DEAD fixable aqua (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... PCR products with no hashtag libraries (spleen, mouse lung, and human lung cells) were combined and purified twice with 0.65× AmPure XP beads (Beckman Coulter) and eluted with 21 μL of nuclease-free water ...
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bioRxiv - Cancer Biology 2020Quote: ... The virally-transduced human CD34+ were sorted for GFP reporter expresssion on a MoFlo high-speed cell sorter (Beckman-Coulter) located at the New York Blood Center ...
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bioRxiv - Immunology 2021Quote: ... as assessed by flow cytometry with Korman orange-conjugated mouse anti-human CD19 monoclonal antibody (mAb) (Beckman Coulter, CA, USA). The purified CD19+ B cells were cultured in the chemical-defined and serum-free X’VIVO 15 medium (Lonza ...
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bioRxiv - Immunology 2023Quote: Human TCR Vβ repertoire analysis was performed using antibodies against the most frequent TCR Vβ variants (covering about 70% normal human TCR Vβ repertoire, as reported by Beckman Coulter “Beta Mark TCR Vbeta Repertoire Kit” (cat.nr. ...
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Rhes protein transits from neuron to neuron and facilitates mutant huntingtin spreading in the brainbioRxiv - Neuroscience 2021Quote: ... The gradient was spun for 3 h at 180,000 × g (average) at 4°C (SW41 Beckman ultra-centrifuge). After the spin the top of the gradient was removed and discarded ...
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bioRxiv - Genomics 2020Quote: ... The PCR products were purified by using 1.3X Agencourt AMPure XP beads (2 or 3 cycles; Beckman Coulter) and evaluated by using an Agilent High Sensitivity DNA Kit on an Agilent 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Cell Biology 2021Quote: ... cell number and volume were measured in a parallel sample using a Multisizer 3 Coulter Counter (Beckman Coulter).
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bioRxiv - Plant Biology 2021Quote: ... Cell density and mean cell diameter were measured using a Coulter Counter (Multisizer 3, Beckman Counter, Brea, CA). For the data in Figure 1A ...
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bioRxiv - Molecular Biology 2022Quote: ... sucrose gradient buffer containing NEB75 or NEB300 and centrifuged at 50,000 rpm for 3 h at 4ºC using a TLS-55 rotor (Beckman). After centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... containing the phosphatases and proteases inhibitors and centrifuged at 100,000xg for 1 h through a 3 ml 1.4 M sucrose cushion using a TL100 centrifuge (Beckman). The crude heavy nuclear fraction and purified nuclei were resuspended in buffer A and extracted with 1% Triton X-100 for 5 min at 4°C (cNM ...
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bioRxiv - Microbiology 2019Quote: ... and adding this to 3 ml sterile inoculum water (Beckman Coulter, Inc. Brea, CA, cat no B1015-2) and 840 μl phenol red solution ...
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bioRxiv - Microbiology 2021Quote: ... The outer membrane vesicles (OMVs) were then isolated by ultracentrifugation (140 000xg, 4°C) for 3 hours (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... Gradients were ultracentrifuged at 28.000g for 3 h at 4°C in a SW 28 Ti rotor (Beckman). 1.13 mL fractions were then collected using the Gradient Station model 153 ...