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Citations for Beckman :
1 - 50 of 140 citations for T 25 Flasks since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The cell lysate was collected in a flask on ice and ultracentrifuged at 4°C for 25 min at 50000 rpm using a Ti-70 rotor (Beckman). The protein purification was done through affinity chromatography on a Ni-column (HiTrap chelating HP column 1 ml ...
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bioRxiv - Genomics 2019Quote: ... prior to sorting naïve T cells (CD4+CD45RO-) and T follicular helper cells (CD4+CD45RO+CD25loCXCR5hiPD1hi) on a MoFlo Astrios EQ (Beckman Coulter).
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bioRxiv - Immunology 2021Quote: ... Residual PE-Cy7+ cells were excluded and EYFP+PE+ (CD4+GzB+ T cells) or PE+CD4+ (Ifng-/-CD4+ T cells) were sorted using MoFlo cell sorter (Beckman Coulter/Dako-Cytomation). CD4+GzB+ T cells (2.8 x 105 cells/animal ...
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bioRxiv - Biophysics 2020Quote: ... transferred into 25×89mm polyallomer ultracentrifuge tubes (Beckman) and ultracentrifuged in an Optima XL-100K ultracentrifuge (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... and 25% (wt/wt in 12.5ml) sucrose gradient in 25 mM citrate buffer in 38 ml Ultraclear tubes (Beckman-Coulter, 344058). The gradient was centrifuged at 100,000 × g for 18 h at 4°C (Beckman-Coulter XPN-80 Ultracentrifuge ...
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bioRxiv - Plant Biology 2022Quote: ... The cDNA was eluted from the beads with 25 µL RNase-free water and purified again with 25 µL (1.0X volume) of Ampure XP beads (Beckman Coulter Life Sciences) then incubated at RT for 15 min then washed and dried as described above ...
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bioRxiv - Microbiology 2022Quote: ... Avanti-J-25 centrifuge (Beckman Coulter, Palo Alto, USA). The viral pellet was resuspended in EBM-2 without supplements ...
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bioRxiv - Biochemistry 2020Quote: ... The flasks were cooled in an ice water bath and cell cultures were centrifuged in a rotor JA-25.50 (Beckman) at 4°C and 4000 x g for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: ... and 500 μl of a 10 ml resuspension of cells from each flask diluted with 19.5 ml Isoton II Diluent (Beckman). PD time ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells in the T25 flask were harvested and counted by the Multisizer 4e coulter counter (Beckman Coulter, Brea, CA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... and centrifuged at 100,000 x g either for 4 hr (hollow fiber bioreactor) or overnight (2D flask culture) in a SW32 rotor (Beckman Coulter). For the preparation of the density gradient ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the lymphocytes fixed (25 μl of Fixative Solution, Beckman Coulter). The samples were mixed and incubated for 10 minutes in the darkness ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysate was centrifuged at 17,000 rpm (JA-25, Beckman Coulter) for 1 hr and the supernatant was filtered using 0.45 µm filter before subjecting it to Ni-NTA resin (Thermo ...
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bioRxiv - Molecular Biology 2020Quote: ... T cells were collected and subjected to FACS (Beckman Coulter, Brea, CA, USA) using the Cell Quest software (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... 72h later CAR-T cells stained with CFSE were analyzed by CytoFLEX (Beckman) with FlowJo 7.6 software.
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bioRxiv - Microbiology 2019Quote: ... The sample was centrifuged in a JA 25-50 rotor (Beckman-Coulter) at 20 Krpm for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was then centrifuged at 17,000 rpm (JA-25, Beckman Coulter) for 1 hr to remove cell debris ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then allowed to grow in flasks for another 48 h before sorting them on a MoFlo Astrios sorter (Beckman Coulter, Pasadena, CA) for the brightest cells ...
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bioRxiv - Immunology 2022Quote: ... Human Vγ9Vδ2 T lymphocytes were phenotyped using PE-labeled anti-NKG2A (#Z199, Beckman Coulter), PE-labeled anti-NKG2D (#ON72 ...
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bioRxiv - Biophysics 2021Quote: ... The diluted solution was centrifuged at 12000g for 10min (Avanti j-25, Beckman). The precipitate was collected and homogenized with an equal mass of a 0.17M NaCl solution and centrifuged again at 12000 g for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 25%-50% sucrose gradient was prepared in 13.2mL ultracentrifuge tubes (Beckman Coulter, 331372) using Biocomp Gradient Master with the following recipe ...
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bioRxiv - Genetics 2020Quote: ... 25%-50% sucrose gradient was prepared in 13.2mL ultracentrifuge tubes (Beckman Coulter, 331372) using Biocomp Gradient Master with the following recipe ...
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bioRxiv - Biochemistry 2023Quote: ... transferred to 25 x 89 mm Ultra-Clear thin-wall tubes (Beckman # 344058) (typically 38 mL per tube ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h using 25 ξ 89 mm polycarbonate tubes (Beckman Coulter, 355618). Pellets were resuspended in 500 μL of sterile phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2024Quote: ... gradient (25 – 39 % OptiPrep, 309600 x g, 5 h, SW60Ti rotor (Beckman Coulter). Purification of VLPs was realized accordingly ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 μM of puromycin) in a 1.5 ml ultracentrifuge tube (Beckman Coulter, 343778) and centrifuged for 1.5 h at 75,000 rpm at 4°C using a TLA-120.2 rotor (Beckman Coulter) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... mRNA was purified from total RNA using poly- T oligo- attached magnetic beads (Beckman Coulter). Fragmentation was carried out using divalent cations under elevated temperatures in NEBNext First Strand Synthesis Reaction Buffer (5X) ...
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bioRxiv - Immunology 2022Quote: ... 25 μL of the second-PCR products were purified using AMPure XP (Beckman-Coulter) at a 0.7:1 ratio (beads-to-sample ...
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bioRxiv - Biochemistry 2019Quote: ... 6 mM MgCl2) in 25×89 mm polypropylene centrifuge tubes (Beckman Coulter, ref. 326823) prepared using the BioComp 107 Gradient Master ...
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bioRxiv - Molecular Biology 2023Quote: The stacked solution was centrifuged for 25 minutes at 3000 × g (Beckman, CA, USA) and 25 ℃ ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were sorted into separate tubes (MoFlow, Beckman Coulter, Brea, CA, USA). A purity of > 98.0% was achieved as monitored by reanalysis of the sorted samples.
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bioRxiv - Immunology 2022Quote: ... S1PR1 expression in CD4+ T cells was assessed by flow cytometry using CytoFlex S (Beckman Coulter).
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bioRxiv - Cell Biology 2024Quote: ... Platelet concentration was measured with the Beckman Coulter T-540 (Beckman Coulter Inc., Brea, CA, USA) hematology analyzer and adjusted to 250 × 106 per mL with Tyrode-HEPES buffer supplemented with 1 mM MgCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... About 9 ml interface that came from 3×SW28 tubes (25×89 mm, Beckman Coulter) was loaded in a SW41 tube (14×89 mm ...
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bioRxiv - Molecular Biology 2020Quote: ... and 4 mM magnesium acetate) were prepared in 25 × 89 mm polyallomer tubes (Beckman Coulter) using a Gradient Master (BioComp) ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 µl of PCR reaction were purified using AMPure XP magnetic beads (Beckman Coulter, USA), eluted in TE buffer and sequenced ...
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bioRxiv - Cancer Biology 2022Quote: ... Fixed cells were incubated with integrin β1-FITC (CD29-FITC, Beckman Coulter, IM0791U, 1:25) and Tomato Lectin Biotinylated (1:250 ...
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bioRxiv - Immunology 2022Quote: Human Vγ9Vδ2 T lymphocytes were stained with FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and APC-labeled anti-human CD3ε mAb (#UCHT1 ...
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bioRxiv - Cancer Biology 2024Quote: ... T cell migration to the lower chamber was quantified using a CytoFLEX flow cytometer (Beckman Coulter, USA).
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bioRxiv - Genomics 2022Quote: ... The ligation reaction is purified by adding 25 µL of Ampure XP beads (Beckman Coulter A63880) to the reaction (1:1 ratio ...
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bioRxiv - Cell Biology 2023Quote: ... and the supernatants were combined and transferred to 25 × 83-mm centrifuge tubes (cat. 344367, Beckman), followed by centrifuge at 95,000g on a SW 32 Ti rotor (Beckman ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentration of the expanding human T cells was calculated on a Coulter Multisizer (Beckman Coulter, Brea, CA) and maintained at 1.0-2.0 × 106 cells per mL in R10 media (RPMI-1640 plus GlutaMAX-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... CAR expression and activation/differentiation of the expanding T cells were monitored by flow cytometry (CytoFLEX cytometer, Beckman Coulter) twice a week (see further) ...
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bioRxiv - Zoology 2021Quote: ... Blood samples were also collected in EDTA for leucocyte counts immediately after collection on a Coulter T-890 (Beckman). EDTA blood ...
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bioRxiv - Cancer Biology 2022Quote: T cell sub-population profiling studies on days 14 and 21 were analyzed using CD3-PC7 (Beckman Coulter, 6607100), CD4-APC-A700 (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... human Vγ9Vδ2 T lymphocytes were collected and stained with a FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and analyzed by flow cytometry ...
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bioRxiv - Immunology 2024Quote: In functional assays proportion of infected CD4+ T cells was address by intracellular staining of p24 (KC57, Beckman Coulter) in combination with anti-CD3 ...
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bioRxiv - Molecular Biology 2021Quote: ... 25°C) and IP-DNA was finally purified from supernatant using AMPure XP magnetic beads (Beckman Coulter). Enrichment was assessed with qPCR for several genomic loci (Table S1) ...
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bioRxiv - Microbiology 2019Quote: ... PCR clean-up: 25 µL PCR product was mixed with AMPure XP beads (Beckman Coulter Genomic, USA), and incubated for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... The seeds were sedimented by centrifugation at 90,000 rpm for 5 min at 25 °C (Beckman Coulter). The microtubule pellet was resuspended in warm BRB80 buffer and microtubule seeds were stored in the dark at room temperature.