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Citations for Beckman :
1 - 50 of 72 citations for Superose 12 Prep Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... using a JA-12 rotor (Beckman) to remove nuclei and unbroken cells ...
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bioRxiv - Molecular Biology 2020Quote: Allegra Benchtop Centrifuge X-12 (Beckman Coulter)
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bioRxiv - Cell Biology 2020Quote: ... centrifuged for 12 minutes in TLA100 (Beckman Coulter) to remove aggregates ...
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bioRxiv - Biochemistry 2023Quote: ... in a 12-mL tube (Polyallomer; Beckman Coulter). Gradients were prepared using a gradient maker (Gradient Master ...
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bioRxiv - Molecular Biology 2023Quote: ... in a 12-mL tube (Polyallomer; Beckman Coulter). Gradients were prepared using a gradient maker (Gradient Master ...
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bioRxiv - Biophysics 2022Quote: ... pH 7.25 buffer in 12 mL ultracentrifuge tubes (Beckman), then applying a preprogramed algorithm to mix the layers using a Gradient Master (BioComp Instruments) ...
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bioRxiv - Cell Biology 2020Quote: ... The Opti-Prep gradients were then centrifuged at 100000 g in a TLS55 (Beckman) or S55S (Hitachi ...
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bioRxiv - Cell Biology 2020Quote: ... followed by high-throughput plasmid prep using a BioMek FXP liquid handling workstation (Beckman Coulter) and Sanger sequencing for insert validation ...
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bioRxiv - Neuroscience 2024Quote: ... The flow-through was then transferred into 12 mL polypropylene tubes (Beckman Coulter) and centrifuged at 140,000xg (Beckman Coulter Optima L100 XP ...
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bioRxiv - Cell Biology 2022Quote: ... This sonicated fraction was transferred to a 12 ml polycarbonate ultracentrifuge tube (Beckman) and adjusted to 40% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... for 12 PCR cycles on the liquid handler Biomek i7 (Beckman Coulter GmbH) at GeneCore ...
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bioRxiv - Cell Biology 2021Quote: ... using 12 mm double sector centerpieces in an AN-50 Ti rotor (Beckman Coulter). Scans were acquired in continuous mode at 280 nm ...
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bioRxiv - Microbiology 2022Quote: ... PCR Enrichment of Adaptor Ligated DNA was run with 12 cycles and SPRI beads (Beckman) were used for library clean up ...
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bioRxiv - Immunology 2021Quote: ... Purified cDNA was amplified for 12 cycles before being cleaned up using SPRIselect beads (Beckman). Samples were diluted 4:1 (elution buffer (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... Purified cDNA was amplified for 12 cycles before being cleaned up using SPRIselect beads (Beckman). Samples were diluted 4:1 (elution buffer (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: ... ChIP DNA was then amplified 12 cycles and purified using AMpure XP beads (Beckman, A63881) twice and then were submitted for150 bp paired-end sequencing on an Illumina novaseq 6000 platform (Novogene).
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bioRxiv - Synthetic Biology 2021Quote: ... Purified cDNA was amplified for 12 cycles before being cleaned up using SPRI-select beads (Beckman). Samples were diluted 4 times in water and ran on a Bioanalyzer (Agilent Technologies ...
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bioRxiv - Immunology 2022Quote: ... For determination of total cell concentrations Flow-12 Count™ Fluorospheres (Beckman Coulter Inc., Krefeld, Germany) was used.
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bioRxiv - Biochemistry 2024Quote: ... 15 µL of the product was mixed thoroughly with 12 µL Ampure XP beads (Beckman Coulter) (0.8× bead/PCR product ratio ...
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bioRxiv - Plant Biology 2024Quote: ... using a standard protocol but with all reaction volumes reduced by 1/10 to accommodate the automation of the prep on the Echo LabCyte liquid handler (Beckman). Libraries were purified using Agencourt Ampure XP beads (Beckman Coulter) ...
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bioRxiv - Immunology 2023Quote: ... Lysis and fixation of whole blood samples were performed by the TQ-Prep Workstation and IMMUNOPREP reagent system (Beckman Coulter). Cells were acquired on a BD FACSymphony A3 Cell Analyzer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Samples were centrifuged for 10 minutes at 3,800 RPM in a JA-12 rotor (Beckman Coulter Inc.) and the bottom layer collected ...
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bioRxiv - Microbiology 2021Quote: ... A iodixanol-SM step gradient was then formed in a 12-mL ultracentrifugation tube (Beckman, ultraclear tubes), by applying 6 mL of a solution at 20% iodixanol-SM ...
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bioRxiv - Microbiology 2024Quote: ... RNA-oligo ligated transcripts were purified in 12 µL volume by AMPure XP (#A63881, Beckman Coulter, USA). Purified RNA was reverse-transcribed using SuperScript II (#18064-014 ...
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bioRxiv - Genomics 2021Quote: ... The nuclease digested DNA (which included both the MN and SN fractions) was directly used for library prep after purification with the Agencourt AMPure XP beads (Beckman Coulter). The MN and SN fractions were separated after sequencing and read mapping using bioinformatics tools as described below ...
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bioRxiv - Microbiology 2020Quote: Library preparation was performed using Illumina NexteraXT™ DNA Sample Prep Kit per manufacturer’s protocol followed by AMPure XP (Beckman Coulter) purification ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was processed using a NEBNext Ultra II Directional RNA library Prep Kit for Illumina paired with a Poly(A) mRNA magnetic isolation module and AMPure XP SPRIselect beads (Beckman Coulter). Libraries were sequenced using a NextSeq 500 High Output sequencer (Illumina ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA was amplified for 12 cycles on a BioRad C1000 Touch thermocycler using SpriSelect beads (Beckman Coulter, USA) and a ratio of SpriSelect reagent volume to sample volume of 0.6 ...
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bioRxiv - Genomics 2021Quote: ... and PCR reactions were performed with 12 cycles and libraries were purified using Agencourt AMPure XP beads (Beckman). Libraries were sequenced on NextSeq 500 High-Output 25bp PE platform (Illumina).
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bioRxiv - Cell Biology 2022Quote: ... 12 ml samples were added to 14 × 89 mm ultra-clear centrifuge tubes (Beckman Coulter, Inc., Brea, CA) and centrifuged at 10,000g and 4°C for 30 minutes (SW41 rotor ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A brief centrifugation up to 4000 g (8 °C, manual stop when reaching 6000 RPM, Beckman JA-12) was used to separate chloroplast and mitochondrial fractions in pellet and supernatant respectively ...
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bioRxiv - Genomics 2021Quote: ... The resulting cDNA was purified and amplified for 12 cycles before cleanup with SPRIselect beads (Beckman, Cat#B23318). Based on the cDNA concentration determined by Qubit (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... 10 ml of viral stock was concentrated in 12 ml polypropylene tubes (14 x 89 mm, Beckman Coulter) by ultracentrifugation (Optima XPN-80 ...
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bioRxiv - Molecular Biology 2024Quote: ... using 12 PCR cylces following manufacturer’s instruction (except for the use of Ampure XP beads (Beckman Coulter, #A63880) instead of SRPIselect beads) ...
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bioRxiv - Genetics 2021Quote: ... DNA fragments ranging from 300 bp to 500 bp were collected using the Pippin Prep (Sage Science, Beverly, MA, USA) and purified using AMPure XP beads (Beckman, Brea, CA, USA). The purified fragments were amplified (six cycles ...
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bioRxiv - Molecular Biology 2020Quote: ... entire ChIPed DNA or 300ng of the input DNA were used to construct libraries using NEBNext® Ultra™ II DNA Library Prep kit with AMPure XP magnetic beads (Beckman Coulter). Library quality and quantity were estimated with Bioanalyzer and Qubit assays ...
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bioRxiv - Cell Biology 2024Quote: ... were collected from microplates and washed twice in PBS and then resuspended in 50 μl of detergent (DNA-Prep Reagent Kit; Beckman Coulter, Brea, CA) containing 10 ng/ml propidium iodide (DNA-Prep Reagent Kit ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was than applied on a glycerol step gradient (30-12% glycerol) and centrifuged for 15 h at 25,000 rpm (SW 32 rotor, Beckman). The separation and distribution of proteins was confirmed by SDS-PAGE gel (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels ...
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bioRxiv - Biochemistry 2020Quote: ... The material was centrifuged for 40 min at 14,000 rpm in a JA-12 rotor (Beckman Coulter, Brea, CA) at 4°C and the supernatant was loaded onto a 1 ml nickel nitrilotriacetic acid-agarose column (Ni-NTA ...
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bioRxiv - Immunology 2021Quote: ... using a 12-parameter panel (CD57-FITC, CD4-PE, CD161-PC7, CD8-KrO, CD279-PC5.5, CD45RA-A700, CD3-APC-A750 [Beckman Coulter] and CD56-BV605 ...
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bioRxiv - Genomics 2024Quote: ... After the End-prep reaction the DNA was purified with 30 μl of AMPure XP beads according to manufactures manual (Beckman Coulter Genomic, CA, USA) and resuspended in 11 μl of nuclease-free water.
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bioRxiv - Genomics 2020Quote: ... according to the manufacturer’s protocol with 12 cycles of PCR followed by two rounds of clean up with 1.3X Agencourt AMPure XP beads (Beckman Coulter). The resulting double-stranded DNAs were fragmented by Covaris sonicator (S220 ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μl M2 eluate was added to the top of a 12 ml 15 - 35 % glycerol gradient and centrifuged in an SW40Ti rotor (Beckman) at 40 ...
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bioRxiv - Neuroscience 2020Quote: ... Gradients were centrifuged at 50,000 rpm and 12°C for 100 minutes in the Optima XE-100 Ultracentrifuge (Beckman Coulter). Next ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was transferred to an ultracentrifuge tube and overlayed with layers of Lysis Buffer A with 12% Ficoll and Buffer B with 8% Ficoll (AAB2209518, Fisher) and centrifuged at 28,500 rpm for 45 min (Beckman 42.1). Afterwards ...
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bioRxiv - Biophysics 2022Quote: T1ST3SC12-(Ndc80)x modules were separated from Ndc80 on a 15-50% glycerol gradient of approximately 12 ml in a SW40 rotor (Beckman) for 16 hrs at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... The V and VI fractions were diluted to a total volume of 12 mL with dfPBS and centrifuged at 140,000 × g for 70 min at 4°C (# Optima-XE SW41 Beckman Coulter), and each pellet were resuspend with 30 μl of dfPBS ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mL samples were supplemented with 500 uL of Optiprep to yield a 20% iodixanol solution and underlayered beneath 1.5 mL layers of 9% and 12% Optiprep/PBS solutions in a 4.9 mL OptiSeal tube (Beckman-Coulter, 362185) using a disposable glass cannula ...
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bioRxiv - Neuroscience 2024Quote: ... at a ratio of 1:2.3 before layering on 3 ml of SCB in a 12 ml Thinwall Ultra-clear centrifuge tubes (Beckman Coulter). Samples were centrifuged at 30,000 xg for 45 min at 4 °C using a SW41 Ti swinging bucket rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... Proteoliposomes were then concentrated by ultracentrifugation (30 min; 100.000 g; 12 ºC) in an Optima Max XP Tabletop Ultracentrifuge (Beckman Coulter), resuspended in Internal Buffer and kept at 12 ºC.