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Citations for Beckman :
1 - 50 of 209 citations for Siglec 15 Human HEK293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Interstitial macrophages (CD45+CD64+Siglec-F−CD11bhigh) or alveolar macrophages (CD45+CD64+Siglec-F+CD11blow) were isolated from C57BL/6 by MOFLO system (Beckman Coulter). Isolated macrophages were cultured for 24 hrs in RPMI-1640 medium containing 10% FBS and 50μM 2-mercaptoethanol with or without GM-CSF (10 ng/ml).
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bioRxiv - Bioengineering 2021Quote: ... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
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bioRxiv - Cancer Biology 2021Quote: ... Aliquots of the same bone biopsy were analyzed by fluorescence in situ hybridization (FISH) and multi-parameter flow cytometry (MFC, Navios Beckman Coulter, Inc.) as parts of the routine clinical diagnosis ...
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bioRxiv - Biochemistry 2020Quote: ... 15 min (Beckman J2-HC Centrifuge). Pellets were transferred to a 50 mL polypropylene Falcon tube and weighed ...
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bioRxiv - Systems Biology 2021Quote: HeLa Kyoto and R1/E mESCs (gifts from Hyman lab, Dresden) and HEK293 (gift from Beckman lab, Berlin), Ptk2 cell lines (gift from Simons lab ...
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bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
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bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
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bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
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bioRxiv - Immunology 2021Quote: Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter). Test sera were diluted 1:40 in growth medium and serially diluted ...
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bioRxiv - Developmental Biology 2023Quote: The anti–human uncoupled primary antibodies used are listed: anti-human CD34 (Beckman, QBEnd/10), anti-human ACE (BB9 ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... CD45-PC7 Ab (clone J33, 1/15, Beckman Coulter), CD3-BV510 Ab (clone HIT3a ...
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bioRxiv - Cell Biology 2019Quote: ... Fluorescence was measured on a Cytoflex 15 (Beckman Coulter) and 10,000 cells per sample were analyzed ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified extracts were prepared by incubating cells with lysis buffer for 15 min on ice followed by centrifugation for 15 min at 16,162 rcf (Beckman Coulter, Microfuge® 16). Extract concentration was quantified by bicinchoninic acid assay (Pierce #23225 ...
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bioRxiv - Immunology 2022Quote: ... anti-human TCR Vβ1 (Beckman Coulter), anti-human CD3 and CD8 antibodies (BD Bioscience).
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bioRxiv - Immunology 2021Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
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bioRxiv - Microbiology 2022Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
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bioRxiv - Biochemistry 2019Quote: ... 10 mM Tris:HCl pH 7.5) and resolved on a 10–40% sucrose gradient in overlay buffer in a zonal rotor (Ti 15, Beckman, 15 h at 21,000 rpm). The peak containing 80S ribosomes was pelleted by centrifugation (19 h at 40,000 rpm ...
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bioRxiv - Immunology 2022Quote: Human Vγ9Vδ2 T lymphocytes were stained with FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and APC-labeled anti-human CD3ε mAb (#UCHT1 ...
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bioRxiv - Bioengineering 2021Quote: ... APC-labelled anti-human CD8 (Beckman Coulter, #IM2469), APC- or PacificBlue-labelled anti-human CD3 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... anti-human CD4-FITC (clone 13B8.2, Beckman Coulter), anti-human CD8a-BV421 (clone RPA-T8 ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C for 15 min using a JLA-9.1000 centrifuge rotor (Beckman). The pellet was resuspended in 70 mL buffer containing 50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... The homogenized solution was centrifuged at 75,000 rpm for 15 min (Beckman, TL100.3). The supernatant was loaded onto a StrepTactin Sepharose column with a volume of 1 mL ...
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bioRxiv - Genomics 2019Quote: ... and using 15 cycles of PCR followed by an Ampure XP bead (Beckman) clean-up.
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bioRxiv - Cell Biology 2023Quote: ... continuous 15-45 % (w/v) sucrose gradients were prepared in SW41 tubes (Beckman) in Polysome Gradient Buffer (10 mM Hepes-KOH ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were loaded on 15-50% sucrose density gradients and centrifuged (Beckman ultracentrifuge) for 1 hour and 30 minutes in a SW41Ti swinging bucket rotor at 39000 rpm at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... or 13 nM anti-human CD81 PE (Beckman Coulter), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... or 13 nM anti-human CD81 FITC (Beckman Coulter) antibody solution ...
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bioRxiv - Immunology 2022Quote: ... FITC anti-human TCR Vβ17 (Beckman Coulter, cat. IM1234), FITC anti-human TCR Vβ21.3 (Backman Coulter ...
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bioRxiv - Immunology 2022Quote: ... FITC anti-human TCR Vβ7.2 (Beckman Coulter, cat. B06666), and Beta Mark TCR Vbeta Repertoire Kit (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: ... Purified cDNA was amplified for 15 cycles and cleaned up using SPRIselect beads (Beckman). Samples were quantified on an Invitrogen Qubit 4 Fluorometer ...
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bioRxiv - Cell Biology 2020Quote: ... for 15 min at 37°C before analysis using CytoFLEX (Beckman Coulter Life Science). Treatment with 10 µM valinomycin for 30 min was included prior to JC-1 incubation as a depolarising control ...
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bioRxiv - Genomics 2020Quote: ... Purified cDNA was amplified for 15 cycles before bead cleanup using SPRIselect beads (Beckman). Samples were quantified using an Invitrogen Qubit 4 Fluorometer ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were harvested by centrifugation for 15 minutes in a JLA-9.1000 rotor (Beckman) at 5,180 x g and the pellets were stored at –20 °C.
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bioRxiv - Cell Biology 2019Quote: ... for 15 minutes at room temperature and analyzed with flow cytometry (Beckman Coulter, Cytoflex). Alternatively ...
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bioRxiv - Molecular Biology 2022Quote: ... After centrifugation at 6000 g for 15 min at 4 °C (JA14 rotor-Beckman), the cell pellet is resuspended with cold lysis buffer (1mg/ml lysozyme ...
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bioRxiv - Genetics 2021Quote: ... 15-60% sucrose gradients were prepared in 89 mm polypropylene centrifuge tubes (Beckman Coulter) using standard settings on a Foxy Jr ...
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bioRxiv - Immunology 2019Quote: ... Lymphocyte counts (4-15 μm) were determined with a Z2 Coulter Counter (Beckman Coulter). For surface staining ...
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bioRxiv - Microbiology 2021Quote: ... and centrifuged each time at 4000 × g for 15 min (Microfuge 20R, Beckman Coulter) followed by resuspension in PBS.
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bioRxiv - Genomics 2023Quote: ... the cDNA was cleaned up and eluted into 15 µL using AMPure beads (Beckman) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: A 15-45% continuous sucrose gradient was made in Thinwall polyallomer tubes (Beckman Coulter) from 15% and 45% sucrose (MP Biomedicals ...
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bioRxiv - Immunology 2020Quote: ... and FITC-conjugated anti-human CD42a mAb purchased from Beckman Coulter (Brea ...
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bioRxiv - Immunology 2022Quote: ... and APC-labeled anti-human CD3ε mAb (#UCHT1, Beckman Coulter), to check the purity of the populations ...
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bioRxiv - Immunology 2023Quote: ... PECy5.5-conjugated anti-human CD117 (104D2D1) was purchased from Beckman Coulter ...
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bioRxiv - Biochemistry 2021Quote: ... The bacterial culture was centrifuged at 4,000 × g for 15 min at 4°C (Beckman) and the cell pellet was resuspended in 30-40 mL of lysis buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... 5000 rpm for 15 min using the Avanti® J-26xp centrifuge (Beckman Coulter®) and resuspended in lysis buffer composed of 50 mM Tris/HCl pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... The lysate was centrifuged at 4 °C for 15 min in a TLA55 rotor (Beckman) at 50,000 rpm to remove insoluble material ...
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bioRxiv - Cell Biology 2020Quote: ... APC-A750 anti-human CD90 (Thy-1/310, #B36121 Beckman Coulter) and Vioblue anti-human CD45 antibodies (REA747 ...
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bioRxiv - Immunology 2023Quote: ... AlexaFluor 700 anti-human CD38 (clone LS198-4-3, Beckman Coulter), APC-Cy7 anti-human CD19 (clone SJ25C1 ...
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bioRxiv - Biochemistry 2022Quote: ... treatment for 15 min at 37°C on an automated Biomek i7 Hybrid system (Beckman Coulter). RNA quantification and quality control was performed on Fragment Analyzer 5300 system and showed an RNA integrity number (RIN ...
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bioRxiv - Microbiology 2021Quote: ... followed by centrifugation for 15 min (14000 x g) at 4 °C (Beckman Coulter, Inc USA). The aqueous phase was transferred to a fresh 1.5 ml tube and the organic phase was kept for deoxyribonucleic acid (DNA ...