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Citations for Beckman :
1 - 50 of 85 citations for Recombinant Human Von Willebrand Factor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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bioRxiv - Neuroscience 2022Quote: ... APC-tagged anti-CD41 (Beckman Coulter).
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bioRxiv - Cell Biology 2024Quote: ... k factor 81.3 (Beckman Coulter). RNA was isolated from the pelleted samples with a miRNeasy micro kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... Centrifugations at 10,000 g (k-factor = 2547.2) and 100,000 g (k-factor = 254.7) were done at +4°C using polyallomer tubes (Beckman Coulter Inc. ...
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bioRxiv - Immunology 2020Quote: ... recombinant protein and CD14 antibody (Beckman) were used at 20 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... recombinant protein and anti-CD14 antibody (Beckman) were used at 20 μg/ml ...
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bioRxiv - Genomics 2019Quote: ... the Hi-C libraries were purified with AMPure XP beads (Beckman Coulter). The concentration of the Hi-C libraries was determined by Bioanalyzer profiles (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... the Hi-C libraries were purified with AMPure XP beads (Beckman Coulter). Quantity and integrity of the Hi-C libraries was determined by Bioanalyzer profiles (Agilent Technologies).
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bioRxiv - Cell Biology 2020Quote: ... EVs were ultracentrifuged (Type 45 Ti rotor, k-Factor 133, Beckman Coulter) with 100000 g for 90 min 4°C ...
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bioRxiv - Genetics 2019Quote: ... The Hi-C libraries were purified with AMPure XP beads (Beckman Coulter, A63881) and sequenced using an Illumina sequencing platform.
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bioRxiv - Genetics 2022Quote: ... and the final Hi-C library was purified with AMPure XP beads (Beckman Coulter). Promoter Capture Hi-C was performed using a customdesign Agilent SureSelect system following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The tagged PCR products were then purified with the Agencourt AMPure XP kit (Beckman Coulter, Switzerland) and eluted in 30 µL DNA/RNA-free water ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... fixed angle Type 45 Ti rotor (k factor = 133; Beckman Coulter, Miami, FL, USA), at 4°C ...
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bioRxiv - Genomics 2023Quote: ... and Hi-C template DNA was purified using 1x AMPure XP beads (Beckman Coulter, A63881) and stored at 4°C until library preparation ...
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bioRxiv - Developmental Biology 2023Quote: The anti–human uncoupled primary antibodies used are listed: anti-human CD34 (Beckman, QBEnd/10), anti-human ACE (BB9 ...
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bioRxiv - Immunology 2022Quote: ... anti-human TCR Vβ1 (Beckman Coulter), anti-human CD3 and CD8 antibodies (BD Bioscience).
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bioRxiv - Immunology 2022Quote: Human Vγ9Vδ2 T lymphocytes were stained with FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and APC-labeled anti-human CD3ε mAb (#UCHT1 ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was centrifuged at 10,500 rpm (∼20,000 × g, k factor = 1,753) for 70 min (SW28 rotor, Beckman). Pelleted lEVs were resuspended in 13.5 ml of PBS (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... and sedimented again at 10,500 rpm (∼20,000 × g, k factor = 1,983) for 70 min (SW40 rotor, Beckman). The supernatant containing sEVs was centrifuged at 25,000 rpm (∼ 110,000 × g ...
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bioRxiv - Cancer Biology 2024Quote: ... and the FOXP3/Transcription Factor Staining Kit (eBioscience) and run on the CytoFlex S cytometer (Beckman Coulter). Analysis was performed in FlowJo.
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bioRxiv - Molecular Biology 2021Quote: ... according to the manufacturer’s instructions using a random nonamer tagged partial p7 sequence (CACGACGCTCTTCCGATCTNNNNNNNNN) and the first strand synthesis product was purified using AMPure XP beads (Beckman) following the manufacturer’s instructions with 1.8 volumes ...
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bioRxiv - Molecular Biology 2019Quote: ... Gradients were centrifuged 18-20h at 192,000g in a SW40 rotor (k-factor 144.5) (Beckman Coulter, Brea, CA). For RNA extraction and small RNA library preparation ...
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bioRxiv - Cell Biology 2021Quote: ... 100K EVs were isolated by ultracentrifugation of 10,000xg supernatant at 100,000xg for 65 min in a SW40 rotor (k-factor 280.3) (Beckman-Coulter). EV-containing 100K ultracentrifugation pellets were resuspended in 20 μL PBS containing 0.2% BSA (cleared from aggregates by ultracentrifugation for 16-20 hrs at 100,000xg) ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and the pellet was resuspended in 25 ∝l lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and Red-C12 fluorescence of the starting material ...
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bioRxiv - Bioengineering 2021Quote: ... APC-labelled anti-human CD8 (Beckman Coulter, #IM2469), APC- or PacificBlue-labelled anti-human CD3 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... anti-human CD4-FITC (clone 13B8.2, Beckman Coulter), anti-human CD8a-BV421 (clone RPA-T8 ...
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bioRxiv - Molecular Biology 2020Quote: ... columns were spun at 100.000 g for 24 hours using a SW-28.1 rotor (k-factor: 276) in an optima XPN-80 ultracentrifuge (Beckman coulter), and subsequently fractions of 1 mL were collected ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 300,000×g at 4°C for 3 h in a TLA-110 fixed angle rotor (k-factor 13) (Beckman Coulter). The pellet was resuspended in 10 µL PBS of which 3 µL was adsorbed onto glow-discharged 2 nm carbon layer coated Quantifoil R1.2/1.3 400 mesh gold grids ...
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bioRxiv - Systems Biology 2023Quote: ... The urine was centrifuged at 20,000 x g for 30 minutes at 4°C (k-factor 1,790) in an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW32 Ti swinging bucket rotor (Rmin 67 ...
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bioRxiv - Evolutionary Biology 2022Quote: Competition assays were performed by using GFP-tagged strains to distinguish between populations with flow cytometry (CytoFLEX Platform Beckman Coulter Life Sciences, USA). Parameters were ...
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bioRxiv - Molecular Biology 2021Quote: ... or 13 nM anti-human CD81 PE (Beckman Coulter), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... or 13 nM anti-human CD81 FITC (Beckman Coulter) antibody solution ...
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bioRxiv - Immunology 2022Quote: ... FITC anti-human TCR Vβ17 (Beckman Coulter, cat. IM1234), FITC anti-human TCR Vβ21.3 (Backman Coulter ...
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bioRxiv - Immunology 2022Quote: ... FITC anti-human TCR Vβ7.2 (Beckman Coulter, cat. B06666), and Beta Mark TCR Vbeta Repertoire Kit (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... The 1 ml concentrates were overlaid on top of the gradient, and centrifuged at 100 000 g (28 000 rpm, k-factor 266) in an Optima L-90K ultracentrifuge (Beckman Coulter) using an SW41 Ti rotor for 18 h at 4 °C with slow deacceleration ...
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bioRxiv - Biophysics 2022Quote: ... EVs were separated from protein aggregates and free reagents by bottom-up density gradient centrifugation in sucrose for 17.30 h at 192,000 g and 4°C using a SW40 rotor (k-factor 144.5; Beckman Coulter, Fullerton, California, USA). Twelve fractions of 1 mL were then collected from the top of the gradient and respective densities were determined by refractometry using an Atago Illuminator (Japan) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were ultracentrifuged at 180,000 × g (38,000 rpm) in a SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter, k-factor 145) for 2 hours at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... The tubes were filled with PBS to reach the final volume and samples were ultracentrifuged at 120,000 g for 60 minutes at 4°C (70.1 Ti rotor, k-factor 36, Beckman Coulter, Brea, CA, USA). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... Virus was concentrated from cell-free culture supernatant by high-speed ultracentrifugation through a 30% sucrose cushion at 80,000xg for 16 hrs in a SW32 rotor (k-factor 321) (Beckman Coulter, Brea, CA).
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bioRxiv - Neuroscience 2023Quote: ... the samples were concentrated to a final volume of 240 µl through ultracentrifugation at 42,300 rpm using a Beckman TLA-55 rotor (RCFavg: 80,160; RCFmax: 110,220; k-factor: 110 and the Optima MAX-XP ultracentrifuge (both Beckman Coulter, Brea, CA, USA) and equal sample volumes were immediately used for the EV Neuro panel A and B (115±25 µl ...
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bioRxiv - Cell Biology 2024Quote: ... while the supernatant was further centrifuged at 118.000 × g (38,800 rpm) (Type Ti 45 fixed-angle rotor, Beckman Coulter, k-factor 178) for 2.5 hours at 4°C to isolate small EVs that were resuspended in PBS and stored at –80°C.
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bioRxiv - Immunology 2020Quote: ... and FITC-conjugated anti-human CD42a mAb purchased from Beckman Coulter (Brea ...
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bioRxiv - Immunology 2022Quote: ... and APC-labeled anti-human CD3ε mAb (#UCHT1, Beckman Coulter), to check the purity of the populations ...
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bioRxiv - Immunology 2023Quote: ... PECy5.5-conjugated anti-human CD117 (104D2D1) was purchased from Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: Dynamic light scattering (DLS) experiments of recombinant GBPs and LPS was performed with Delsa Max Pro instrument (Beckman Coulter). GBPs were diluted in buffer C or buffer C (apo ...
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bioRxiv - Microbiology 2020Quote: EV-depleted medium was obtained by overnight ultracentrifugation of DMEM supplemented with 20% FBS at 100,000xg in a Type 45 Ti rotor (Beckman Coulter, K-factor 1042.2). After ultracentrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... The gradients were centrifuged at 45,000 rpm for 16 hours at 4 °C using an Optima™ XPN-80 centrifuge with SW60 Ti rotor (average RCF of 207,976 ×g, maximum RCF of 272,840 ×g, κ-factor of 81; Beckman-Coulter, Fullerton, United States). After centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... and centrifuged at 16,500 × g (14,500 rpm) at 4°C for 20 min using a Type Ti 45 fixed-angle rotor (Beckman Coulter, k-factor 1.275) to isolate large EVs ...
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bioRxiv - Cell Biology 2020Quote: ... APC-A750 anti-human CD90 (Thy-1/310, #B36121 Beckman Coulter) and Vioblue anti-human CD45 antibodies (REA747 ...