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Citations for Beckman :
201 - 250 of 298 citations for Recombinant Human CD19 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... and measurements were conducted using a CytoFLEX-S (V-B-Y-R) instrument (Beckman Coulter) by analyzing 80 µL of each replicate ...
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bioRxiv - Biochemistry 2021Quote: ... The labeled peptides were further purified by C-18 (5 μm, 4.6 mm × 150 mm, Beckman Coulter Inc.) reverse phase-high performance liquid chromatography ...
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bioRxiv - Immunology 2023Quote: ... and intracellularly stained with the Fluorescein isothiocyanate-labeled KC57 (anti-HIV-1 p24) antibody (1:200) (Beckman Coulter) or a mixture of both antibodies ...
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bioRxiv - Immunology 2021Quote: ... using a 12-parameter panel (CD57-FITC, CD4-PE, CD161-PC7, CD8-KrO, CD279-PC5.5, CD45RA-A700, CD3-APC-A750 [Beckman Coulter] and CD56-BV605 ...
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bioRxiv - Cell Biology 2020Quote: ... APC-A750 anti-human CD90 (Thy-1/310, #B36121 Beckman Coulter) and Vioblue anti-human CD45 antibodies (REA747 ...
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bioRxiv - Immunology 2023Quote: ... AlexaFluor 700 anti-human CD38 (clone LS198-4-3, Beckman Coulter), APC-Cy7 anti-human CD19 (clone SJ25C1 ...
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bioRxiv - Molecular Biology 2023Quote: Proteins were collected by centrifugation (Beckman-Coulter JA-14 rotor ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with the fluorescently labeled anti-p24 antibody (Clone: KC57, Beckman Coulter, 6604667; 1/200 dilution).
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bioRxiv - Cell Biology 2019Quote: ... and the amount of released [35S]-labeled N-formyl-methionine was measured using a scintillation counter (Beckman Coulter, Inc.) in 3.5 ml of Econo-Safe scintillation cocktail (RPI) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were analyzed by flow cytometry weekly prior to functional assays by using anti-pan γδ TCR-PE (Beckman Coulter), anti-αβTCR-FITC (eBioscience) ...
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bioRxiv - Microbiology 2021Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution) in combination with Nef or Vpu rabbit antisera (1:1000 dilution) ...
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bioRxiv - Immunology 2020Quote: ... PE-CD11b and FITC-CD3 (all Ebioscience) for 20 minutes and washed before sorting using a Moflo Astrios (Beckman Coulter) or FACSAria IIu (BD BioSciences) ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with following antibody mix for 30 minutes on ice: pan-γδTCR-PE (1:10; IMMU510; Beckman Coultier) and CD69-APC (1:25 ...
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bioRxiv - Microbiology 2023Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA, 1:100 dilution). Flow cytometry measurements were performed using a FACSCalibur (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
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bioRxiv - Microbiology 2023Quote: ... PBS-washed cells were PFA-fixed for 90 minutes and immunostained with the following antibodies diluted in 0.1% Triton X-100 in PBS: anti-HIV-1-core-antigen-FITC or -PE (#6604665 or #6604667; Beckman Coulter), rabbit-anti-human-MX1/2 (#sc-166412 ...
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bioRxiv - Immunology 2020Quote: ... while human B cells were CD38+/- (Beckman Coulter #6699531; 1µl per test) and CD19 (BD #557791 ...
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bioRxiv - Microbiology 2019Quote: ... DNA from the labeled and unlabeled samples was added into separate quick seal 5ml tubes (Beckman Coulter, Indianapolis, IN, USA) combined with 1.88 g/ml CsCl and gradient buffer for a final buoyant density of 1.725 g/ml ...
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bioRxiv - Microbiology 2020Quote: ... DNA from the labeled and unlabeled samples was added into separate quick seal 5ml tubes (Beckman Coulter, Indianapolis, IN, USA) combined with 1.88 g/ml CsCl and gradient buffer for a final buoyant density of 1.725 g/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Protein aggregates were pelleted by ultracentrifugation of the proteins individually at 80,000 rpm in TLA 100 (Beckman Coulter) for 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... with anti-Met-PE (clone eBioclone 7, eBiosciences) or isotype control (clone eBRG1, eBiosciences) or permeabilized (IntraPrep Permeabilization kit, Beckman Coulter) before staining (total Met) ...
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bioRxiv - Immunology 2020Quote: ... The solution of antibody + S1 trimer and ACE2-beads was allowed to equilibrate for 2 hours before the amount of Dy-549P1 associated with the beads was detected using the PE channel on the CytoFLEX S (Beckman Coulter) cytometer ...
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bioRxiv - Immunology 2022Quote: ... following manufacturer’s instructions with the following p24 antibodies: HIV-1 p24 clone 28B7-APC (Medimabs) and HIV-1 p24 clone KC57-PE (Beckman Coulter). Acquisition was performed on the Fortessa LSR flow cytometer (BD Bioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... washed twice in PBS and the intensity of the dsRed expression was measured at the PE channel CytoFLEX LX (Beckman Coulter) and the data was analyzed using the FlowJo software (Tree Star Inc ...
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bioRxiv - Immunology 2023Quote: ... These were chosen to stain splenocytes and lymphocytes from the NALT of the same mouse for the following TCR Vβ segments (clone, brand, fluorophore) to identify any overlap of clonotypes: VB1 (BL37.2, Beckman Coulter, PE), VB2 (RE654 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with 1% FBS in PBS antibody staining buffer and stained with the following antibodies: CD64 PE-Cy7 (Cat. # B06025, Beckman Coulter); CD45 PerCP (Cat ...
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bioRxiv - Microbiology 2023Quote: ... H-2K(b)-p79 or H-2D(b)-p56 MHC-peptide complex was provided as dextramers conjugated to PE or APC (Immundex, Fairfax, VA).The data was collected on a CytoFLEX flow cytometer (Beckman Coulter) and analyzed using FlowJoX v10.0.7 (Treestar Inc. ...
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bioRxiv - Microbiology 2022Quote: ... After centrifugation of the suspension for 10 min at 5000 g (Beckman Coulter Allegra X30-R Centrifuge), the supernatant was decanted and the wet solid was washed four times (resuspended in autoclaved MilliQ water ...
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bioRxiv - Biochemistry 2020Quote: ... and the amount of released [35S]-labeled dipeptide was measured for each time point using a scintillation counter (Beckman Coulter, Inc.).
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Permeabilized cells were then incubated with 50 μl of 1:100 dilution of phycoerythrin (PE)-conjugated mouse anti-p24 monoclonal antibody (KC57-RD1; Beckman Coulter, Inc.) for 30 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... After detection using Streptavidin-PE-Cy7, samples were fixed (IC fixation buffer, eBioscience, USA) and acquired on a CytoFLEX flow cytometer (Beckman Coulter, USA). Lectin staining was quantified after doublet and live/dead exclusion using the geometric mean fluorescence intensity (MFI) ...
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bioRxiv - Immunology 2022Quote: ... and anti-human CD64 antibodies (APC) using a CytoFlex S (Beckman Coulter, USA). All the antibodies were purchased from SinoBiological ...
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bioRxiv - Immunology 2020Quote: ... and with Cyto-stat tetrachrome (FITC anti-CD45, PECy5 anti-CD3, PE anti-CD4, and ECD anti-CD8, Beckman Coulter, catalog number 660713). Unstimulated cells and pokeweed mitogen-stimulated cells were additionally stained with AF750 anti-CD19 (Beckman Coulter ...
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bioRxiv - Genomics 2019Quote: Human RNA libraries were individually cleaned using Agencourt AMPure XP beads (Beckman Coulter, #A63881) and a double-sided procedure (left 1×-right 0.6×) ...
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bioRxiv - Molecular Biology 2020Quote: Binding to CD16 was studied by a competitive assay with an anti-CD16-PE/3G8 antibody conjugated to phycoerythrin (Beckman Coulter, Brea, CA, USA) and unlabelled test and control antibodies using Jurkat-CD16 cells.
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bioRxiv - Bioengineering 2022Quote: ... Amnion-derived MSCs from placental tissue were isolated and characterized with CD73-APC, CD90-FITC and CD105-PE-Cy7 (all from eBioscience, San Diego, CA) by flow cytometry (CytoFLEX XL, Beckman Coulter GmbH, Krefeld, Germany) [21] and HUVECs were isolated from the umbilical cord and characterized as published previously [25].
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bioRxiv - Biochemistry 2020Quote: ... The labeled and unlabeled polymerized actin samples were pelleted down by ultracentrifugation at room temperature (45000 rpm for 1 hour, Beckman TLA-55 rotor). The pellets were rinsed once with 1 mL G-buffer and re-suspended into ice cold 0.5 mL G-buffer ...
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bioRxiv - Biophysics 2022Quote: ... The cell suspension was centrifuged for 5 min at 2000 g (Centrifuge Beckman Coulter Allegra X-15-R, Rotor Sx4750A)) and 4°C ...
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bioRxiv - Microbiology 2019Quote: ... 50 ml of culture was harvested by centrifugation for 10 min at 3000 rpm (Beckman Coulter Allegra X30-R Centrifuge) and washed two times with T-R minimal medium ...
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bioRxiv - Cancer Biology 2024Quote: ... the ratio of the number of fluorescently labeled cells to the total number of cells was determined by flow cytometry (Beckman, Brea, CA, United States).
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bioRxiv - Microbiology 2020Quote: ... The proteins were sedimented in the SW40Ti rotor (Beckman Coulter) at 100,000 × g for 17 h at 4 °C ...
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bioRxiv - Microbiology 2022Quote: Supernatant containing proteins and membrane were ultracentrifuged at 200,000xg (Beckman Coulter Ultracentrifuge ...
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bioRxiv - Biochemistry 2020Quote: ... proteins and liposomes were mixed in polycarbonate tubes (Beckman Coulter) and incubated for 30 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... another 2 mL of staining bacterial samples was analyzed with a flow cytometry to check cell viability (Beckman CytoFLEX FCM, USA, Channel FL2-A: PE-A).
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bioRxiv - Immunology 2019Quote: ... To identify KIR2DS1 negative donors, NK cells were stained with mAbs to KIR2DL1 (R&D, 143211) and KIR2DL1/S1 (Beckman Coulter, EB6) as described (Fauriat et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Samples are then resuspended in PBS and incubated with APC-A700 anti-human CD56 (N901, #B92446 Beckman Coulter), APC-A750 anti-human CD90 (Thy-1/310 ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentration of the expanding human T cells was calculated on a Coulter Multisizer (Beckman Coulter, Brea, CA) and maintained at 1.0-2.0 × 106 cells per mL in R10 media (RPMI-1640 plus GlutaMAX-1 ...
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bioRxiv - Immunology 2022Quote: ... GEM-RT products were subsequently amplified using human BCR primers and cleaned up through SPRIselect beads (Beckman Coulter). Next ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of Caco-2 cells and human ileum organoids was extracted using RNAdvance Tissue kit (Beckman Coulter) and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research) ...
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bioRxiv - Bioengineering 2023Quote: ... or by staining with anti-human TCR mAbs (Vβ13.1-IMMU 222, Beckman Coulter for A2/NY A97L-TCR). Cell staining was performed at 4°C for 30min.