Labshake search
Citations for Beckman :
51 - 100 of 560 citations for Rat Insulin Like Growth Factor Binding Protein 6 IGFBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... The library was cleaned up and size-selected by two consecutive rounds of binding to 1.2x and 0.8x SPRI beads (Agentcourt AMPure XP beads, Beckman Coulter). The integrity and size distribution of the final libraries (two biological replicates of KCl-treated and one of untreated ...
-
bioRxiv - Genomics 2021Quote: ... Estradiol (E2) and Progesterone assays are competitive binding immunoenzymatic assays performed on the Unicel DXI 800 system (Beckman Coulter). FSH assay is based on Microparticle Enzyme Immunoassay (MEIA ...
-
bioRxiv - Microbiology 2022Quote: ... 400 μL of 1X binding buffer was then added before analysing using a CytoFLEX S flow cytometer (Beckman Coutler). Annexin V-FITC positive cells were designated as early apoptotic ...
-
bioRxiv - Biochemistry 2023Quote: ... binding reactions were centrifuged at 50,000 RPM for 30 min at 25 °C in a TLA-100 rotor (Beckman). Following centrifugation ...
-
bioRxiv - Biochemistry 2023Quote: ... binding reactions were centrifuged at 50,000 RPM for 30 min at 25 °C in a TLA-100 rotor (Beckman). Promptly after centrifugation ...
-
bioRxiv - Immunology 2021Quote: ... 39 mL of cGAMP-VLP-containing supernatant was gently overlaid on 6 mL of cold PBS containing 20% sterile filtered endotoxin free sucrose in 6 Ultra-Clear tubes (Beckman Coulter, ref 344058), and centrifuged for 1 hour and 30 minutes at 100,000 g 4°C ...
-
bioRxiv - Genomics 2023Quote: Rat tissues were disrupted in GenFind v2 lysis buffer (Beckman Coulter, Indianapolis, IN) with a tissue ruptor (Omni International ...
-
bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and the pellet was resuspended in 25 ∝l lysis buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and Red-C12 fluorescence of the starting material ...
-
bioRxiv - Molecular Biology 2022Quote: ... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
-
bioRxiv - Genomics 2023Quote: Sheared DNA was purified by binding to an equal volume of AMPure XP beads (A63881, Beckman Coulter, Inc. Brea, CA) and eluted in 50µl of 10mM Tris-HCl ...
-
bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II, respectively); and βHCG and AFP CMIA-ARCHTECT I 4000.The assays were performed as recommended by the manufacturers ...
-
bioRxiv - Molecular Biology 2020Quote: ... columns were spun at 100.000 g for 24 hours using a SW-28.1 rotor (k-factor: 276) in an optima XPN-80 ultracentrifuge (Beckman coulter), and subsequently fractions of 1 mL were collected ...
-
bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 300,000×g at 4°C for 3 h in a TLA-110 fixed angle rotor (k-factor 13) (Beckman Coulter). The pellet was resuspended in 10 µL PBS of which 3 µL was adsorbed onto glow-discharged 2 nm carbon layer coated Quantifoil R1.2/1.3 400 mesh gold grids ...
-
bioRxiv - Systems Biology 2023Quote: ... The urine was centrifuged at 20,000 x g for 30 minutes at 4°C (k-factor 1,790) in an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW32 Ti swinging bucket rotor (Rmin 67 ...
-
bioRxiv - Cell Biology 2019Quote: ... was added to the growth medium and parasitemia was monitored every 24hrs using a CyAn ADP (Beckman Coulter) or CytoFLEX (Beckman Coulter ...
-
bioRxiv - Biochemistry 2021Quote: ... 6 mM 2-mercaptoethanol and centrifuged with a 70Ti (Beckman coulter) rotor at 40,000 rpm for 18 hours at 4°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5×10^6 cells counted by Multisizer 4e Coulter Counter (Beckman) were plated on a 60 mm plate and serum starved before analysis unless otherwise indicated ...
-
bioRxiv - Cell Biology 2020Quote: ... The 1 ml concentrates were overlaid on top of the gradient, and centrifuged at 100 000 g (28 000 rpm, k-factor 266) in an Optima L-90K ultracentrifuge (Beckman Coulter) using an SW41 Ti rotor for 18 h at 4 °C with slow deacceleration ...
-
bioRxiv - Biophysics 2022Quote: ... EVs were separated from protein aggregates and free reagents by bottom-up density gradient centrifugation in sucrose for 17.30 h at 192,000 g and 4°C using a SW40 rotor (k-factor 144.5; Beckman Coulter, Fullerton, California, USA). Twelve fractions of 1 mL were then collected from the top of the gradient and respective densities were determined by refractometry using an Atago Illuminator (Japan) ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were ultracentrifuged at 180,000 × g (38,000 rpm) in a SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter, k-factor 145) for 2 hours at 4°C ...
-
bioRxiv - Biochemistry 2021Quote: ... Binding reactions were spun at 300,000 g for 30 min at 25 °C using Optima MAX-TL ultracentrifuge (Beckman Coulter, Brea, CA). Bundling reactions were spun at 17,000 g for 15 min at 25 °C ...
-
bioRxiv - Bioengineering 2024Quote: ... the stained cells were washed twice to remove any unspecific binding antibodies and were then subjected to measurement using a Beckman Cytoflex S flow cytometer (Beckman, CA, USA). The resulting data were analyzed using FlowJo software (Tree Star ...
-
bioRxiv - Genetics 2019Quote: ... The relative growth rate was determined by counting cells over the 3 day period using Coulter Counter Z2 (Beckman).
-
bioRxiv - Microbiology 2021Quote: ... Cell growth was determined either by measuring samples in a Multisizer 4e Coulter counter (Beckman Coulter Inc., California, USA) or using an Infinite M200Pro (TECAN Austria GmbH ...
-
bioRxiv - Biochemistry 2023Quote: ... Bacterial growth was quantified by the optical density at 600 nm (OD600) using a Spectrophotometer (Beckman Coulter, DU-800) or an incubated microplate reader (Tecan Spark).
-
bioRxiv - Systems Biology 2023Quote: The growth of cultures was monitored as increase in cell number using a Coulter Counter model Z2 (Beckman Coulter). Cell size analysis was performed using a Coulter Z2 Particle Cell Analyzer (Beckman-Coulter) ...
-
bioRxiv - Immunology 2023Quote: ... The OD was read at 450 nm using an automatic ELISA plate reader (DTX 880, Multimode detector; Beckman Coulter).
-
bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with an additional 400 µl 1× Binding Buffer and analyzed within 20 min by CytoFLEX S (Beckman Coulter Life Science). At least 1 × 104 cells were analyzed to determine the percentage of apoptotic cells ...
-
bioRxiv - Cancer Biology 2020Quote: ... 320 μL of 1X binding buffer was added to each sample before analysis on the EPICS XL flow cytometer (Beckman Coulter, Buckinghamshire, UK).
-
bioRxiv - Biophysics 2022Quote: ... The tubes were filled with PBS to reach the final volume and samples were ultracentrifuged at 120,000 g for 60 minutes at 4°C (70.1 Ti rotor, k-factor 36, Beckman Coulter, Brea, CA, USA). Finally ...
-
bioRxiv - Cell Biology 2021Quote: ... Virus was concentrated from cell-free culture supernatant by high-speed ultracentrifugation through a 30% sucrose cushion at 80,000xg for 16 hrs in a SW32 rotor (k-factor 321) (Beckman Coulter, Brea, CA).
-
bioRxiv - Neuroscience 2023Quote: ... the samples were concentrated to a final volume of 240 µl through ultracentrifugation at 42,300 rpm using a Beckman TLA-55 rotor (RCFavg: 80,160; RCFmax: 110,220; k-factor: 110 and the Optima MAX-XP ultracentrifuge (both Beckman Coulter, Brea, CA, USA) and equal sample volumes were immediately used for the EV Neuro panel A and B (115±25 µl ...
-
bioRxiv - Cell Biology 2024Quote: ... while the supernatant was further centrifuged at 118.000 × g (38,800 rpm) (Type Ti 45 fixed-angle rotor, Beckman Coulter, k-factor 178) for 2.5 hours at 4°C to isolate small EVs that were resuspended in PBS and stored at –80°C.
-
bioRxiv - Genomics 2023Quote: Rat tissue powders were further disrupted using Agencourt RNAdvance tissue lysis buffer (Beckman Coulter, Brea, CA) with a tissue ruptor (Omni International ...
-
bioRxiv - Microbiology 2019Quote: The density of the culture (A600) after cell growth was tested using a spectrophotometer (DU730, Beckman Coulter, Placentia, California, USA). The concentrations of the solvents (acetone ...
-
bioRxiv - Microbiology 2022Quote: Cell growth was measured based on the absorbance of the culture at A600 (OD600) using a spectrophotometer (DU730; Beckman Coulter). The fermentation products were assayed as previously reported (19) ...
-
bioRxiv - Microbiology 2023Quote: ... After ON growth at 37° C with shaking strain frequencies were measured using flow cytometry (Beckman Coulter CytoFLEX flow cytometer). Relative fitness was calculated as the ratio of relative frequency of the strains at the end of the experiment to their initial frequency.
-
bioRxiv - Synthetic Biology 2024Quote: ... 6 mM MgCl2) with 0.7 M sucrose in Ti-45 tubes (Beckman-Coulter). Ribosomes were pelleted by centrifugation at 27,000 rpm (57,000 xg ...
-
bioRxiv - Molecular Biology 2020Quote: Binding to CD16 was studied by a competitive assay with an anti-CD16-PE/3G8 antibody conjugated to phycoerythrin (Beckman Coulter, Brea, CA, USA) and unlabelled test and control antibodies using Jurkat-CD16 cells.
-
bioRxiv - Microbiology 2020Quote: EV-depleted medium was obtained by overnight ultracentrifugation of DMEM supplemented with 20% FBS at 100,000xg in a Type 45 Ti rotor (Beckman Coulter, K-factor 1042.2). After ultracentrifugation ...
-
bioRxiv - Molecular Biology 2023Quote: ... The gradients were centrifuged at 45,000 rpm for 16 hours at 4 °C using an Optima™ XPN-80 centrifuge with SW60 Ti rotor (average RCF of 207,976 ×g, maximum RCF of 272,840 ×g, κ-factor of 81; Beckman-Coulter, Fullerton, United States). After centrifugation ...
-
bioRxiv - Cell Biology 2024Quote: ... and centrifuged at 16,500 × g (14,500 rpm) at 4°C for 20 min using a Type Ti 45 fixed-angle rotor (Beckman Coulter, k-factor 1.275) to isolate large EVs ...
-
bioRxiv - Molecular Biology 2022Quote: Procyclic form cell lines for depletion of either TbPH1 or TbKifX2A were induced at a density of 2×106 cells/ml with 1 µg/ml doxycycline and growth monitored daily with a Z2 Coulter Counter Analyzer (Beckman Coulter). Cells were diluted back to the initial cell density every day ...
-
bioRxiv - Microbiology 2023Quote: ... After six days of photoautotrophic growth the culture was centrifuged for 30 minutes at 4,500 rpm to concentrate the biomass (Allegra X-22R, Beckman Coulter, USA). The wet biomass was then divided into 20 mL serum bottles sealed with butyl-rubber septa ...
-
bioRxiv - Immunology 2020Quote: ... recombinant protein and CD14 antibody (Beckman) were used at 20 μg/ml ...
-
bioRxiv - Molecular Biology 2023Quote: Proteins were collected by centrifugation (Beckman-Coulter JA-14 rotor ...
-
bioRxiv - Cell Biology 2020Quote: ... amplified by PCR for 6 cycles and purified with AMPure XP beads (Beckman Coulter). The final libraries were pooled and sequenced using the onboard cluster method ...
-
bioRxiv - Genomics 2020Quote: ... (6) the library fragments were purified with AMPure XP Beads (Beckman Coulter, Shanghai, China) to select the insert fragments of 150-200 bp in length ...
-
bioRxiv - Biophysics 2022Quote: ... The 6 kb long 64x repeat was then purified using SPRIselect beads (Beckman Coulter) at a 1 to 0.4 v/v ratio ...