Labshake search
Citations for Beckman :
251 - 300 of 672 citations for Rat Endothelin 2 EDN2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... Protein-liposome complexes were separated from unbound protein by centrifugation (2 h at 45.000 rpm, 4 °C, MLS50 rotor -Beckman Coulter) in the density gradient ...
-
bioRxiv - Biochemistry 2021Quote: ... The depleted fraction was collected by gentle centrifugation (100 rcf for 2 min) into a clean 96 well plate (Beckman Coulter ...
-
bioRxiv - Microbiology 2022Quote: ... clarified using 0.2 µm filter and pelleted at 54,000 rpm (180,000 ×g) for 2 hours at 4 °C in a TLA-55 rotor in an Optima Max-XP tabletop ultracentrifuge (Beckman). The supernatant was removed ...
-
bioRxiv - Bioengineering 2022Quote: ... the conditioned media was subjected to an additional centrifugation step of 118,000 × g for 2 h in a Type 70 Ti ultracentrifuge rotor (Beckman Coulter). The resulting EV pellet was resuspended in 1X PBS and placed into a Nanosep 300 kDa MWCO spin column (Pall ...
-
bioRxiv - Cell Biology 2022Quote: ... the virus-cell mixture was centrifuged at 1,000 rcf for 2 hours at 32°C (Beckman Coulter, Allegra X-30R). Infected cells were then cultured as described above and subsequently FACS-sorted for the establishment of clonal cell lines.
-
bioRxiv - Microbiology 2022Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 µL of RNAase free water ...
-
bioRxiv - Cell Biology 2023Quote: ... Each fraction was subjected to a washing step in ice-cold PBS at 100,000 × g at 4°C for 2 h using a SW28Ti rotor (Beckman Coulter). The pelleted EVs were resuspended in ice-cold PBS ...
-
bioRxiv - Immunology 2022Quote: ... eVLPs were purified by ultracentrifugation at 50,000 rpm (135,000 x g) for 2 hours at 4°C using a TLA100.3 rotor and a OptimaTM TLX ultracentrifuge (Beckman Coulter) on a 20% w/v sucrose cushion ...
-
bioRxiv - Neuroscience 2023Quote: ... The top 11 mL and the bottom 2 mL of the gradient were transferred to separate 60 mL 45Ti ultracentrifuge tubes (Beckman), topped up with PBS and centrifuged at 100,000 x g for 70 min at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... The filtrate was ultracentrifuged at 100,000 ×g at 4 °C for 2 h using an Optima XE-90 (Beckman Coulter) to collect EMVs ...
-
bioRxiv - Neuroscience 2022Quote: ... DNase-treated RNA was subsequently purified using RNase-free Agencourt AMPure XP beads (Beckman Coulter; 2:1 volume of beads:sample).
-
bioRxiv - Cell Biology 2023Quote: ... 5 mM Mg(OAc)2) and centrifuged for 20 min at 100,000 x g in a 70Ti rotor (Beckman Coulter). The resulting membrane pellet was resuspended in 1 ml rough microsome (RM ...
-
bioRxiv - Plant Biology 2023Quote: ... and water to a final volume of either 2 μL for the acoustic liquid handling robot (Labcyte Echo 550, Beckman) or 5 μL for manual handling ...
-
bioRxiv - Cancer Biology 2023Quote: ... Filtered supernatants were concentrated by ultracentrifugation at 22,000 rpm for 2 hours at 4°C with OptimaTM L-90K Ultracentrifuge (Beckman Coulter) and stored at -80°C (never refrozen).
-
bioRxiv - Biophysics 2023Quote: ... the supernatant of cell lysates was centrifuged to isolate the total membrane fraction at 24,000 rpm for 2 h in the 45Ti rotor using ultracentrifuge (Beckman-Coulter). Membrane resuspension in 50 mM Tris-HCl buffer (pH 8.0 ...
-
bioRxiv - Cell Biology 2023Quote: ... The supernatant was then overlaid onto a 2 ml 60% iodixanol cushion and spun at 100,000 x g (SW32 rotor, Beckman Coulter) for 18h ...
-
bioRxiv - Neuroscience 2023Quote: ... This supernatant was next subjected to ultracentrifugation at 120,000 x g for 2 h at 4LJ in a SW 41 Ti Rotor Swinging-Bucket rotor (Beckman Coulter), and the pellets collected as small factors like exosome ...
-
bioRxiv - Physiology 2023Quote: ... Samples were ultracentrifuged at 100,000 rpm at 16°C for 2 hours (rotor: Beckman TLA-100; Centrifuge: Beckman Optima TLX). 100ul from the top of the tube was collected as VLDL fraction ...
-
bioRxiv - Plant Biology 2023Quote: ... The resulting solution was then centrifuged again at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Avanti J-20TM centrifuge, Beckman Coulter). The supernatant was decanted ...
-
bioRxiv - Plant Biology 2023Quote: ... The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). The supernatant was then decanted and discarded ...
-
bioRxiv - Microbiology 2023Quote: ... resuspended in 200 µl 2% PBS-BSA and analyzed for mean fluorescence intensity (MFI) of ACE2 expression with CytoFLEX flow cytometer (Beckman). To determine the biological effects of the purinergic receptor P2X7 and NLRP3 inflammasome on viral entry ...
-
bioRxiv - Biophysics 2023Quote: ... followed by layering the cleared lysate onto 40 mL of 2 M sucrose cushion and ultracentrifugation in Ti-45 rotor (Beckman) at 42,000 rpm for 2 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... Fraction LP2 was obtained after centrifugation of fraction LS1 for 2 h at 165,000 g in a SW40 Ti rotor (Beckman, US). Corresponding supernatant was collected as Fraction LS2 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Virus particles were collected by centrifugation at 32,000rpm for 2 hours at 4°C using the Optima XE-90 ultracentrifuge (Beckman Coulter). Virus was aliquoted and stored at -80°C until further use ...
-
bioRxiv - Developmental Biology 2023Quote: ... The resulting supernatant was subjected to ultracentrifugation at 120,000 × g at 4°C for 2 h (Optima Max TL, Beckman Coulter). Pellets were resuspended in 1 ml cold PBS and centrifuged at 120,000 × g at 4°C for 2 h ...
-
bioRxiv - Immunology 2023Quote: ... and purified and concentrated by ultracentrifugation on a 20% sucrose cushion (24,000 rpm and 4°C for 2 hours with a SW32Ti or SW28 rotor (Beckman Coulter)) ...
-
bioRxiv - Cell Biology 2023Quote: ... The samples were sedimented by velocity centrifugation at 36,000 x rpm for 2 h at 4°C using SW40Ti rotor in Optima L-80XP ultracentrifuge (Beckman Coulter). Absorbance at 254 nm was measured from lower to higher sucrose gradients using an ISCO gradient fractionation system and the optical density at 254 nm was continuously recorded with a Foxy JR Fractionator (Teledyne ISCO).
-
bioRxiv - Cell Biology 2024Quote: ... The same amounts of proteins were loaded on a double sucrose cushion (20% and 30% sucrose) and centrifuged at 190,000 g for 2 h at 4 °C in an Optima L-100XP ultracentrifuge (Beckman–Coulter). Extracts were incubated with pre-washed anti-Flag beads (Sigma ...
-
bioRxiv - Immunology 2023Quote: The SARS-CoV-2 lentiviral pseudotyped neutralization assays were performed on integrated automation platforms consisting of a Biomek liquid handler from Beckman Coulter ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were ultracentrifuged at 4 °C and 150,000 ˗ g for 2 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) and supernatants were removed ...
-
bioRxiv - Cancer Biology 2023Quote: ... The gradients were ultracentrifuged at 35,000 rpm for 2 hrs at 4°C using an SW41 Ti rotor (Beckman Coulter). The gradients were then displaced into a UA-6 continuous UV detector (Teledyne ISCO ...
-
bioRxiv - Cell Biology 2024Quote: ... Gradients were centrifuged at 36,000 rpm for 2 h at 4 °C in an Optima L-100XP ultracentrifuge (Beckman–Coulter). Following centrifugation ...
-
bioRxiv - Developmental Biology 2021Quote: ... and cleaned up with SPRIselect Reagent Kit (Beckman Coulter, Pasadena, CA).
-
bioRxiv - Genomics 2019Quote: ... with cleanup in between steps using SPRIselect Reagent Kit (Beckman Coulter). The Post-ligation product was then amplified with a C1000 Touch Thermal Cycler during the Sample Index PCR ...
-
bioRxiv - Microbiology 2020Quote: ... and purified with the AMPure XP Kit (Beckman Coulter GmbH, USA), quality checked using Agilent High Sensitivity DNA Kit (Agilent ...
-
bioRxiv - Cell Biology 2021Quote: ... We used PerFix-nc Kit (Cat.No. B31167; Beckman Coulter GmbH, Germany) and Phalloidin-Atto-647 (Atto-Tec GmbH ...
-
bioRxiv - Immunology 2022Quote: ... After amplification and purification with SPRIselect Reagent Kit (Beckman Coulter, B23318) of RT products ...
-
bioRxiv - Immunology 2022Quote: ... and Beta Mark TCR Vbeta Repertoire Kit (Beckman Coulter, cat. IM3497). The Vbeta Repertoire Kit contains 24 different anti-TRBV antibodies that come in 8 vials (A – H) ...
-
bioRxiv - Immunology 2022Quote: ... After amplification and purification with SPRIselect Reagent Kit (Beckman Coulter, B23318) of RT products ...
-
bioRxiv - Cell Biology 2021Quote: ... followed by 1,2X AMpure beads purification kit (Beckman Coulter, Brea, California) to keep only retro-transcribed single strand cDNA ...
-
bioRxiv - Plant Biology 2021Quote: ... and then purified using an Agencourt AMPure Purification Kit (Beckman Coulter). Purified cDNA was quantified by a Qubit 3.0 Fluorometer (Life Technologies ...
-
bioRxiv - Cancer Biology 2020Quote: ... The enzymatic fragmentation and size selection (SPRIselect Reagent Kit, Beckman Coulter) were used to optimize the cDNA amplicon size followed by adding P5 and P7 sample indexes (used in Illumina sequencers) ...
-
bioRxiv - Immunology 2019Quote: ... fixed and permeabilized using the PerFix EXPOSE kit (Beckman Coulter, #B26976) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: ... with cleanup in-between steps using SPRIselect Reagent Kit (Beckman Coulter). Post-ligation product was amplified with a T1000 Thermal Cycler programed at 98 °C for 45 s ...
-
bioRxiv - Immunology 2020Quote: ... pS6 staining was performed using a PerFix EXPOSE kit (Beckman Coulter). For evaluation of intracellular targets ...
-
bioRxiv - Pathology 2019Quote: Intracellular staining was performed using the Intraprep™ kit (Beckman Coulter). Flow cytometry analysis were performed on a BD Pharmingen LSRFortessa® cytometer ...
-
bioRxiv - Microbiology 2020Quote: ... DNA was then purified using AMPure XP beads kit (Beckman Coulter) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... gDNA was further purified using Agencourt AMPure XP Kit (Beckman Coulter). Then the purity ...
-
bioRxiv - Plant Biology 2023Quote: ... purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics, USA) and analyzed by capillary electrophoresis ...
-
bioRxiv - Immunology 2022Quote: ... Amplified products were purified with 0.8x SPRIselect Reagent Kit (Beckman Coulter) and eluted in 45µL nuclease-free water ...