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Citations for Beckman :
451 - 500 of 560 citations for Rabbit Anti Borrelia burgdorferi sensu stricto B31 CRASP 2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... was subjected to centrifugation at 100,000g for 2 h in an Optima MAX-XP benchtop ultracentrifuge with a TLA-100.3 rotor (Beckman Coulter, Inc., Brea, CA). Pellets were resuspended in 100 µl PBS and stored at −80 °C.
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bioRxiv - Pathology 2020Quote: ... Hemocytes stained with 4’-6-diamidino-2-phenylindole (DAPI) were analyzed on a CytoFLEX flow cytometer with CytExpert software (Beckman-Coulter, USA). Plots of side scatter SSC-A versus forward scatter FSC-A were used for visualization of cell groups ...
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bioRxiv - Biochemistry 2019Quote: ... in iso-osmolar RB150/Mg2+ (containing a reduced level (2%) of glycerol) and 80µl were transferred to 7 × 20 mm polycarbonate tubes (Beckman Coulter, Brea CA), overlaid with 80µl of 35% ...
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were layered on top of 1 ml of 0.85 M sucrose in 2 mM EGTA and centrifuged at 75,000 g for 30 min in a Beckman FW-27 rotor (Beckman, Fullerton, CA, USA). This step was repeat twice ...
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bioRxiv - Microbiology 2021Quote: ... soil was thoroughly mixed with 20 ml of 0.01 M CaCl2 for 30 min on a Elmi Intelli-Mixer RM-2 (Elmi Tech Ltd, Latvia) and subjected to centrifugation in an Allegra X-22R centrifuge (Beckman Coulter, USA) at 3900 rcf for 20 min and to remove inorganic S (Dedourge et al. ...
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bioRxiv - Microbiology 2023Quote: ... the prepared DNA library was size-selected on a 2% agarose gel (410–470 bp) using the Agencourt SPRI system to reduce mitochondrial contamination (Beckman Coulter, Inc.). After library prep ...
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bioRxiv - Cell Biology 2023Quote: ... cells were blocked in 2% human serum and PBS for 30 min at 4°C and stained with DC-SIGN (Beckman Coulter #A07407), CD80 (BioLegend #305214) ...
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bioRxiv - Plant Biology 2023Quote: ... 700 μl of obtained extract was applied to a chilled sucrose density gradient and centrifuged at 4 °C for 2 hours 45 minutes at 151,000 ×g using the SW 41 Ti rotor (Beckman Culture Life Sciences). After centrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were loaded onto the sucrose gradients and centrifuged at 40’000 rpm for 2 h at 4°C using the SW 41 Ti rotor (Beckman Coulter, Cat# 331362). Subsequently ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 100,000 × g for 2 hr at 4 °C (Type 50.2 Ti Fixed angle ultracentrifuge rotor, Beckman Coulter, Brea, CA, USA). The supernatants were aspirated ...
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bioRxiv - Immunology 2023Quote: ... Bacteria cultures were collected and discarded by centrifugation for 60 min at 4,000-8,000 x g and the supernatants were subjected to high-speed centrifugation at 11,9000 x g for 2-3 h at 4°C (Beckman Coulter Optima Ultracentrifuge). The pellets containing the OMVs were washed with PBS ...
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bioRxiv - Plant Biology 2023Quote: ... DNA extracts were purified using a bead clean up method with a 2:1 ratio of Ampure XP beads (Beckman Coulter, USA) to DNA elution ...
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bioRxiv - Biophysics 2023Quote: ... The virions were concentrated by ultracentrifugation through a 20% (w/v) sucrose cushion (2 h at 28,000 rpm in a SW32 rotor [Beckman Coulter Life Sciences]). The pellet was resuspended in PBS ...
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bioRxiv - Genetics 2023Quote: ... in-house VLPs were produced using the same protocol followed by ultracentrifugation at 24,000 RPM for 2 hours (SW 28 Beckman swinging bucket rotor) to concentrate the VLPs and avoid diluting the HSPCs culture media ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged at 60,000 rpm at 4°C for 2 h on an SW65 Ti ultracentrifuge rotor (Beckman Coulter, California, USA). The pellets were gently washed by dispensing and removing 500 µl of ice-cold RNase-free water ...
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bioRxiv - Microbiology 2022Quote: ... The immunoturbidimetric test was used to assess serum complement (C) and immunoglobulin antibodies (AU5421; Beckman Coulter, USA). Immunoblotting and immunofluorescence were used to detect serum autoantibodies ...
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bioRxiv - Immunology 2024Quote: Antibodies and clones against the following proteins were used (clone, fluorophore, company): CD3 (UCTH1, PECy5, Beckman Coulter), CD7 (Horizon V450 or BUV395 ...
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bioRxiv - Immunology 2021Quote: ... including PE-Cy7-conjugated mouse anti-human CD45 (Beckman Coulter, CA, USA), efluor660-conjugated rat anti-human OCT3/4 (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... a combination of plate-bound anti-CD3 (Beckman Coulter REF # IM-1304) and soluble anti-CD28 (Beckman coulter REF # IM1376 ...
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bioRxiv - Microbiology 2021Quote: ... and anti-HIV-1 p24 clone KC57-RD1 (Beckman Coulter, CA, USA). The secondary goat anti-mouse and anti-rabbit Abs conjugated to Alexa488 ...
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bioRxiv - Microbiology 2020Quote: ... staining with anti-HIV-1 Core Antigen Clone KC57 FITC (Beckman Coulter) and detection of HIV-1-infected cells by flow cytometry ...
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bioRxiv - Cancer Biology 2019Quote: ... - anti-MIR300 and -TUG1shRNA oligonucleotides (ODNs) (Beckman Research Institute, City of Hope), 1.25 μg/ml anti-TGFβ neutralizing antibody (1D11 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with anti-CD19-PC5.5 (J3-119, Beckman Coulter), Live/Dead Aqua (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 E7335S, Index Primers Set 2, E7500S) with Agencourt AMPure XP magnetic beads (Beckman coulter A63881) exactly as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and extracellular vesicles including exosomes and virions were pelleted by ultracentrifugation at 141,000 g at 4 °C for 2 h in a swinging-bucket rotor (SW28; Beckman Coulter, Brea, CA, USA). The pellet was resuspended in filtered PBS and centrifuged at 18,300 g and 4°C for 10 min to remove insoluble aggregates ...
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bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was overlaid onto a 20% (w/v) sucrose cushion in Tris-NaCl-EDTA buffer and centrifuged at 134,000 g for 2 hours (Beckman Coulter 30 Ti rotor). The resulting viral pellet was suspended in VCM by repeat pipetting and aliquots stored in cryovials at minus 70°C until needed ...
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bioRxiv - Genomics 2020Quote: ... were layered onto a 1.8M sucrose cushion and ultra-centrifuged at 24,400 rpm at 4°C for 2 hours using a SW28 swinging bucket rotor (Beckman Coulter Optimal centrifuge #L90K). The nuclear pellet was re-suspended in 2% BSA in 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... EMVs were obtained by ultracentrifugation from 4 mL of filtrate at 100,000 × g (average centrifugal force) and 4°C for 2 h with an OptimaX centrifuge (Beckman Coulter, Brea, CA, USA). The pellets were resuspended in 400 μL of Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... The tubes were sealed and centrifuged at 50,000 rpm at 4°C for 2 h in an OptimaTM L-90K ultracentrifuge using the 70.1Ti rotor (Beckman Coulter, Brea, CA, USA). After the centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μM or 10 μM from a 2 mM or 10 mM stock solution using a Biomek FX with 384-well head (Beckman Coulter, Indianapolis, IN). The DMSO concentration was kept below or at 0.5% v/v ...
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bioRxiv - Physiology 2022Quote: ... the library preparation step followed the CEL-Seq 2 protocol85: the RT product was digested by ExoI and HinFI and purified using AMpure XP beads (Beckman Coulter™, USA). Then ...
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bioRxiv - Microbiology 2023Quote: ... then the colonies were diluted and inoculated into the Neg MIC EN-2J panel containing β-lactams listed in Table 2 and incubated in MicroScan Walkway40 Plus (Beckman Coulter Inc.). Breakpoint criteria for susceptible (S) ...
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bioRxiv - Biochemistry 2020Quote: Sedimentation velocity experiments were performed using a ProteomeLab XL-1 analytical ultracentrifuge with the use of an AN60-Ti rotor and Epon-2 channel centerpieces (Beckman Coulter, Brea, CA, USA). Protein samples were diluted with SANS buffer to concentrations yielding an absorbance of 0.5 at 280 nm as measured with an 8454 UV–Vis spectrophotometer (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... The success of the amplifications was checked in a 2% agarose gel and PCR products were subsequently purified using Agencourt AMPure XP magnetic beads kit (Beckman Coulter, Brea, CA, USA), quantified with the QuantiFluor™ dsDNA System (Promega ...
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bioRxiv - Molecular Biology 2019Quote: ... The concentrated urine sample was resuspended in 3.2 ml of a 50% iodixanol solution and layered on the bottom of a 17 ml Thinwall Polypropylene Tube (Beckman Coulter, Fullerton, California, USA). A discontinuous DG was prepared by additional layering of 4 ml of 20% ...
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bioRxiv - Neuroscience 2019Quote: ... and 1□mM DTT) before a centrifugation process of 2 h 20 min at 30,000□× g (Beckman J-25 centrifuge with a J13.1 rotor). The supernatant was carefully aspirated and the extracted nuclei in a form of a pellet were resuspended in a nuclear storage buffer (15% sucrose ...
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bioRxiv - Biochemistry 2019Quote: ... and 5 mM MgCl2 at pD 7.5 corresponding to a pH reading of 7.1) at 25°C as follows: spin column at 2000 rpm for 2 min in a tabletop centrifuge (Beckman Coulter Allegra R Tabletop Centrifuge), discard the flow-through ...
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bioRxiv - Microbiology 2020Quote: ... and layered on top of a two-step sucrose gradient (20%/50% w/v in HNE) and centrifuged for 2 h at 154 000 x g at 4 °C in a SW41 rotor (Beckman Coulter, Woerden, the Netherlands) to clear from cell debris ...
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bioRxiv - Microbiology 2023Quote: The amplicons obtained with each of the strategies (using pools 1 and 2) were pooled in each group and purified with one volume of AMPure XP beads (Beckman Coulter, Brea, CA, USA). The beads were washed with 80% ethanol ...
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bioRxiv - Biochemistry 2023Quote: ... cells were treated with the drugs (20 µM to a suspension at 500,000 cells/mL in PBS containing 2 % FBS at 37°C) and directly analyzed by flow cytometry (Gallios, Beckman-Coulter, 405/488 nm laser) over 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... using an analytical ultracentrifuge XL-I with an Anti-60 rotor (Beckman Coulter) and double-sector cells (Nanolytics ...
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bioRxiv - Microbiology 2019Quote: ... Intracellular Gag staining was carried out using a Gag monoclonal antibody conjugated to Phycoerythrin (KC-57 RD1 Beckman Coulter). 1×105 cells from a HIV GPV-zip coded library were washed once with FACS buffer and fixed with 100 µl of BD cytofix for 10 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2022Quote: ... The cells were stained with a panel of monoclonal antibodies containing: ECD CD16 clone 3G8 (Beckman Coulter; cat.no. A33098), BV711 CD45 clone HI30 (BD Biosciences ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... and then stained for flow cytometry using the CD34-phycoerythrin (PE) antibody (PE-pool, Beckman Coulter, IM1459U, Brea, CA). RNA-sequencing libraries were then prepared from RNA isolated from the bulk mutant AML bone marrow cells and the normal CD34+ bone marrow cells using TruSeq Stranded Total RNA kit (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... unbound PKH67 dye and unbound antibodies by overnight bottom-up sucrose density gradient (SDG) ultracentrifugation at 192,000 g (SW40 rotor Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: The TCR V beta repertoire kit contained antibodies to over 76% of V βeta families (Beckman Coulter, Fullerton, CA). The cells were stained with these antibodies in combination with tetramers or dextramers for 20 minutes at RT to determine the TRBV repertoire of antigen specific cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Platelet concentration was obtained by flow cytometry using an antibody against human CD41 labelled with PC7 (6607115, Beckman Coulter) and MP-count beads (6607115 ...
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bioRxiv - Cancer Biology 2020Quote: ... Filtered media containing lentiviral particles were centrifuged at 24000rpm at 20°C for 2 hrs in 90Ti rotor in ultra-centrifuge (Beckman Coulter Optima TM L-100K). Viral particle pellet was obtained after discarding supernatant post centrifugation and suspended in 100 μl OptiMEM media for overnight at 4°C.The collected lentivirus suspension was used for transfection or stored for later use at −80°C ...
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bioRxiv - Physiology 2020Quote: ... one bird per pen in Experiment 2) and centrifuged for 5 min at 17013 × g using a Beckman microfuge (Model E 348720, Beckman Instruments, INC, Palo Alto, CA). Then the viscosity of ileal supernatant was measured using a Brookfield cone-plate digital viscometer (Model LVDV-Ⅲ ...
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bioRxiv - Cancer Biology 2020Quote: ... isotype controls for intracellular stains and subjected to post-acquisition compensation using VersaComp Antibody Capture Bead Kit (Beckman Coulter #B22804). Data was analyzed using Kaluza Software (Beckman Coulter) ...