Labshake search
Citations for Beckman :
351 - 400 of 706 citations for Progesterone Metabolites ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... 1.6 ml of 35% (w/v) sucrose and 5% (w/v) sucrose in MBS were layered in a 5.1 ml ultracentrifuge tube (Beckman Coulter, #326819) to form a discontinuous sucrose gradient ...
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bioRxiv - Cell Biology 2022Quote: ... After ultracentrifugation at 200,000 x g for 5 h at 4°C (SW55Ti rotor in a XL-90 Beckman Coulter centrifuge), fractions containing purified EVs or purified phages were extracted from the top following the scheme in Supp ...
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bioRxiv - Molecular Biology 2022Quote: ... The gradients were prepared using freeze-thaw method42 in Thinwall Ultra-Clear ultracentrifuge tubes (5 ml, 13×51 mm; Beckman-Coulter). To create the 50% sucrose cushion ...
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bioRxiv - Microbiology 2022Quote: The purified AdgI/AdgH complex sample was diluted to 0.5 mg/ml using the sample buffer containing 25 mM Tris-HCl pH 7.5 and 150 mM NaCl and subjected to the sedimentation velocity measurements using an Optima XL-I analytical ultracentrifuge (Beckman-Coulter). Data was analyzed with the SEDFIT and SEDPHAT programs33,34.
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bioRxiv - Immunology 2022Quote: ... Cells were pelleted at 400xg for 5 min and washed with PBS twice before flow cytometry (Beckman Coulter CytoFLEX Flow Cytometer). Analysis of phagocytic ability was undertaken using FCS Express.
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bioRxiv - Cell Biology 2022Quote: ... and the pellet was resuspended with 5 ml buffer B and transferred into a 14 ml ultracentrifuge tube (331372, Beckman Coulter). 9 ml Buffer C (1.8 M sucrose ...
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bioRxiv - Genetics 2023Quote: ... STR DNA fragments were amplified using a pair of specific primers with 5’ terminal parts of P5 and P7 Illumina adapters (Table S1) and purified by 100% (v/v) SPRIselect beads (Beckman Coulter). In the second step ...
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bioRxiv - Molecular Biology 2022Quote: Nuclei were isolated from 200 cotyledons of 5-day-old seedlings and purified using a two-layer Percoll gradient at 3000 g before staining with 0.5 μM DAPI and sorting by FACS according to their ploidy levels using a MoFlo Astrios EQ Cell Sorter (Beckman Culture) in PuraFlow sheath fluid (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... The live singlets were counted using DAPI 1:10 dilution by mixing 45 μl of cell suspension with 5 μl DAPI by gating on FSC-A vs FSC-H measuring average event/ μl on Cytoflex (Beckman Coulter). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... 0.2% Triton X-100 and 100 µg/mL chloramphenicol) and ultracentrifuged at 42500 rpm for 5 h in type 70 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... ice-cold 5 mL 2.5 M sucrose was added to the bottom of an open-top plastic tube (Beckman-Coulter 344058), carefully topped up with ice-cold 5 mL 1.875 M sucrose ...
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bioRxiv - Molecular Biology 2024Quote: ... bottom layer was made by diluting OptiPrep™ solution with clarified HM or digesta sample (2.25 mL OptiPrep™ + 0.75 mL sample = 3 mL) into a 5 mL open-top thin wall ultra-clear tube (Beckman Coulter). A discontinuous gradient was made with 40% (w/v) ...
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bioRxiv - Plant Biology 2023Quote: ... for 15 minutes at 20°C followed by 5 minutes at 65°C for inactivation of the enzymes and purified with 1X AMPure XP beads (Beckman Coulter); 1D2 Adapters (ONT ...
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bioRxiv - Microbiology 2024Quote: ... incubated at 25°C and 5% CO2 (PF) or 37°C and 5% CO2 (BF) for 60 min and analyzed using a CytoFLEX flow cytometer (Beckman Coulter). Events (10,000 ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatant was incubated in a boiling water bath for 5 min and centrifuged for 10 min at 40,000 rpm (Beckman Ti45 rotor). Supernatant was supplemented with ammonium sulfate (1 g per 6.1 ml) ...
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bioRxiv - Neuroscience 2024Quote: ... were loaded onto 10–50% continuous sucrose density gradients in polysome buffer (20 mM HEPES pH 7.4, 150 mM KCl, 5 mM MgCl2) (Gradient Master™, BioComp) and centrifuged in an SW40Ti swing-out rotor (Beckman Coulter) at 250,000 g for 2 h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... pooled and purified with Agencourt AMPure XP kit (Beckman Coulter). Purified PCR products were quantified using a Qubit dsDNA HS assay kit (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... and Agencourt® RNAClean® XP Kit (Beckman Coulter, USA). Measurement of RNA yield and quality was performed using Qubit® RNA HS assay kit (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... and Agencourt® RNAClean® XP Kit (Beckman Coulter, USA). Measurement of RNA yield and quality was performed using Qubit® RNA HS assay kit (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted using the GenFind v2 kit (Beckman Coulter) and libraries prepared using the NexteraFlex kit (Illumina Inc. ...
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bioRxiv - Microbiology 2022Quote: ... and the Agencourt RNAdvance Tissue Kit (Beckman Coulter, Krefeld, Germany). FFPE sections were loaded into a microTUBE-130 Screw-Cap (Covaris ...
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bioRxiv - Cancer Biology 2022Quote: ... single-stained beads (VersaComp Antibody Capture Bead Kit; Beckman Coulter) and a single-stained cell-mix of all samples analyzed were used ...
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bioRxiv - Bioengineering 2021Quote: ... were performed using the PerFix-nc Kit (Beckman Coulter #B31167), for 30 min at 4 °C in supplemented PBS containing 2 mM EDTA and 2% FBS ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted using the RNAdvance Tissue kit (Beckman Coulter) and the KingFisher Flex System (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... preamplification and purification with SPRIselect Reagent Kit (Beckman Coulter, B23318). Paired V(D)J sequence were enriched with 10X primers ...
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bioRxiv - Microbiology 2023Quote: ... purified with the Agencourt AMPure XP kit (Beckman Coulter Genomics), and analyzed by capillary electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... and cleaned using the Agencourt RNAClean XP Kit (Beckman-Coulter) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Samples were purified with the CleanSEQ kit (Beckman Coulter, A29151) following the Agencourt CleanSEQ Dye-Terminator Removal protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Before FACsorting we added the 5 ul of the cellular death dye Draq7TM (Viability dye, Far-red DNA intercalating agent, Beckman Coulter, #B25595). The suspension was sorted on an Astrios II cell sorter (Beckam Coulter) ...
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bioRxiv - Molecular Biology 2019Quote: ... 4 ml of 10 % and 3.5 ml of 5 % iodixanol in a 17 ml Thinwall Polypropylene Tube (Beckman Coulter, Fullerton, California, USA). The DG was centrifuged 18 h at 100,000 g and 4 °C using SW 32.1 Ti rotor (Beckman Coulter ...
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bioRxiv - Plant Biology 2021Quote: ... cesium chloride continuous density gradient in resuspension buffer and centrifuged at 34,000 m for 5 hours at 20 °C using an SW41 Ti rotor (Beckman Coulter, Brea, CA). The virus particles were observed in the lower of two pale white bands in the gradient ...
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bioRxiv - Microbiology 2019Quote: The 5-10 mL bioaerosol samples were concentrated by spinning at 3000 × g for 10 min (Allegra® X-15R, Beckman Coulter). The pellet was resuspended in 200 µl fresh 7H9 and stained overnight (12-16 h) ...
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bioRxiv - Microbiology 2019Quote: ... The unbound dye is removed by centrifugation (14,000 rpm for 5 minutes at 4 °C) (Beckman J2-MC Centrifuge, Beckman Coulter, Brea, USA) and washed with 1 mL of M9 buffer ...
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bioRxiv - Neuroscience 2019Quote: ... iodixanol solution and overlaid with 7.0 mL of 25% (v/v) iodixanol and 350 µL of 5% (v/v) iodixanol in a polyalomar tube (Beckman, Miami, FL). All of the above iodixanol solutions were prepared in lipid flotation buffer (10 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... were loaded onto 5-25% (w/v) linear sucrose gradients and centrifuged in a Beckman Optima L-90K Ultracentrifuge (Beckman SW40 rotor) at 40,000 rpm for 3 h 15°C ...
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bioRxiv - Genomics 2021Quote: ... with 5 PCR reactions per column then pooled together and cleaned up a second time with 1.6X SPRI beads (Beckman Coulter, Brea, California). The CHEQ-seq vector containing a random 17 bp BC upstream of the synthetic intron was linearised via inverse PCR with primers “CHEQseq_lin_A_For” and “CHEQseq_lin_A_Rev” or “CHEQseq_lin_B_For” and “CHEQseq_lin_B_Rev” ...
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bioRxiv - Neuroscience 2022Quote: ... was loaded onto a sucrose gradient made up of 5 ml 1.2 M sucrose on the bottom and 5 ml of 0.8 M sucrose at the top in thin wall ultracentrifugation tubes (Beckman Coulter, Cat # 344059). The loaded gradient was ultracentrifuged at 69,150 x g for 1 h at 4 °C (SW 41 Ti Swinging-Bucket Rotor ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 mL of 5% solution in a 14 x 89 mm (13.2 mL) thin walled ultra-clear centrifuge tubes (Beckman Coulter, Cat. # 344059). Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor ...
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bioRxiv - Cancer Biology 2023Quote: ... Conditioned media were centrifuged at 1000 rpm for 5 min to remove cell debris and then either added to Ultra-Clear Quick-Seal ultracentrifuge tubes (Beckman Coulter 246.344322), 100 kDa molecular weight cut-off centrifugal filters (MWCO ...
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bioRxiv - Microbiology 2024Quote: ... Liposomes and liposome-protein complexes were separated from unbound protein by ultracentrifugation of the reaction mixture in 5 mL Optiprep™ (Axis-Shield PoC AS) density gradients for 1 hour at 40,000 ×g (SW55Ti Beckman Coulter rotor). The liposome-protein mixture was adjusted to 34% Optiprep concentration in a volume of 300 μl ...
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bioRxiv - Cell Biology 2024Quote: ... and cells were isolated by centrifugation at 500 g for 5 min at 4°C followed by being treated with a red blood lysis buffer (Beckman Coulter, USA). After washing with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with 5 μg/ml PI (ST1569-10mg, Beyotime) and immediately analyzed using the CytoFLEX flow cytometer (Beckman Coulter, USA). Tumor cells were distinguished based on GFP positivity or CD45 negativity ...
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bioRxiv - Developmental Biology 2021Quote: ... and cleaned up with SPRIselect Reagent Kit (Beckman Coulter, Pasadena, CA).
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bioRxiv - Genomics 2019Quote: ... with cleanup in between steps using SPRIselect Reagent Kit (Beckman Coulter). The Post-ligation product was then amplified with a C1000 Touch Thermal Cycler during the Sample Index PCR ...
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bioRxiv - Microbiology 2020Quote: ... and purified with the AMPure XP Kit (Beckman Coulter GmbH, USA), quality checked using Agilent High Sensitivity DNA Kit (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... We used PerFix-nc Kit (Cat.No. B31167; Beckman Coulter GmbH, Germany) and Phalloidin-Atto-647 (Atto-Tec GmbH ...
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bioRxiv - Immunology 2022Quote: ... After amplification and purification with SPRIselect Reagent Kit (Beckman Coulter, B23318) of RT products ...
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bioRxiv - Immunology 2022Quote: ... and Beta Mark TCR Vbeta Repertoire Kit (Beckman Coulter, cat. IM3497). The Vbeta Repertoire Kit contains 24 different anti-TRBV antibodies that come in 8 vials (A – H) ...
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bioRxiv - Immunology 2022Quote: ... After amplification and purification with SPRIselect Reagent Kit (Beckman Coulter, B23318) of RT products ...
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bioRxiv - Cell Biology 2021Quote: ... followed by 1,2X AMpure beads purification kit (Beckman Coulter, Brea, California) to keep only retro-transcribed single strand cDNA ...