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Citations for Beckman :
201 - 250 of 296 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... Whole-cell lysates were spun at 35k rpm for 45 min in a Type 45 Ti rotor (Beckman Coulter), and the supernatant was decanted and filtered through a 0.45 μm cellulose acetate membrane ...
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CM1-driven assembly and activation of Yeast γ-Tubulin Small Complex underlies microtubule nucleationbioRxiv - Biochemistry 2021Quote: ... Lysate was cleared by ultracentrifugation at 40,000 x g for 30 min in a Type 45Ti rotor (Beckman-Coulter). Xrcc4-Spc110164-207 was then purified by NiNTA affinity chromatography followed by addition of 3C protease overnight at 4° C to cleave the 6His tag ...
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bioRxiv - Neuroscience 2019Quote: ... Then the lysates were spin down at 67,000 rpm for 1 hour at 18 °C with the ultracentrifuge (Beckman). The AAV particles were collected by aspirating the interface between 40-60% iodixanol with a syringe ...
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bioRxiv - Cell Biology 2020Quote: ... After heating the lysate was allowed to cool and then centrifuged at 164,700 × g (Optima XPM ultracentrifuge, Beckman Coulter) for 45 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 183,960 x g for 88 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Genetics 2020Quote: ... 5 μL of each PCR reaction from the single worm lysate was pooled and purified by SPRI (Beckman Coutler) to remove unincorporated oligos ...
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bioRxiv - Biophysics 2022Quote: ... Cells were lysed by sonication and the lysate was clarified by centrifugation at 18,000 rpm (25,000 xg JA-20 rotor, Beckman-Coulter) for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Then the lysates were spin down at 67,000 rpm for 1 hour at 18 °C with the ultracentrifuge (Beckman). The AAV particles were collected by collecting the interface between 40 and 60% iodixanol with a syringe ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate was centrifuged at 40,000 rpm for 50 min at 4°C (45 Ti fixed-angle rotor, Beckman) and the transporter-Fab complex was isolated by affinity chromatography using Strep-Tactin resin ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 mL of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was then clarified (using a JA 25.50 Beckman rotor at 40000x g for 30 min, 4°C) and the soluble fraction was incubated with 2 ml of Glutathione Sepharose 4B resin for 2h at 4°C under constant agitation ...
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bioRxiv - Cell Biology 2023Quote: ... Cell debris was pelleted by spinning lysates at 70,000 rpm for 45 min at 4ºC in a Ti70 in an ultracentrifuge (Beckman Coulter). In a cold room ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei were passed through a 40um diameter strainer and the lysate was transferred into a 15ml ultracentrifuge tube (Beckman). 9ml of ice cold sucrose solution (30.78g Sucrose ...
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bioRxiv - Biochemistry 2023Quote: ... lysates were centrifuged at 60,000 x g (4 °C) for 45 min (Beckman Avanti JXN-26 (rotor: JA 25.5)) and immediately supplemented with 10 mL pre-washed Ni2+-NTA agarose (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 minutes in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 2 ml of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Bioengineering 2022Quote: ... After addition of 450µL of Agencourt RNAdvance Tissue lysis buffer (Beckman Coulter, A32646) the capsules were homogenized using a FastPrep-24 (MP Biomedicals) ...
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bioRxiv - Microbiology 2022Quote: ... the preparations were further processed with the Agencourt RNAdvance Tissue Kit (Beckman Coulter) with the KingFisher™ Flex Purification System (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: Rat tissues were disrupted in GenFind v2 lysis buffer (Beckman Coulter, Indianapolis, IN) with a tissue ruptor (Omni International ...
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bioRxiv - Genomics 2023Quote: ... we extract genomic DNA from earpunch tissue using the Agencourt DNAdvance Kit (Beckman Coulter Life Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by ultracentrifugation at 30,000 × g 30 min at 4°C using a Ti45 rotor (Beckman Coulter) twice ...
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bioRxiv - Biophysics 2020Quote: ... lysate was centrifuged at 5 °C with 13000 rpm for 15 min by Avanti J-E Ultra Centrifuge (Beckman Coulter). Supernatant from centrifugation was filtered with 0.45 μm SFCA syringe filters (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... The total bacterial lysate was centrifuged at 48,384 x g at 4 °C for 1 hr (Beckman JA-25.50 rotor), and the supernatant was applied to a 5 mL HisPur Ni-NTA resin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The cell lysate was clarified via centrifugation at 66,000 x g for 30 min in a Type 70 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... lysate was collected and centrifuged at 3000x rpm for 20 minutes to pellet cell debris (Beckman Coulter Allegra X-14R). This procedure was repeated for a second passage working stock with collection at 48hpi and titered by TCID50 assay.
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bioRxiv - Molecular Biology 2022Quote: ... The lysate was clarified at 503,000 rcf for 45 min at 4°C using a Type 70 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Cell Biology 2020Quote: ... Cleared supernatants obtained from cytoplasmic lysates were loaded on a linear 15%-50% sucrose gradient and ultracentrifuged in a SW41Ti rotor (Beckman) for 1 h and 40 min at 180,000 x g at 4°C in a Beckman Optima LE-80K Ultracentrifuge ...
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bioRxiv - Biophysics 2021Quote: ... Cells were lysed and the lysate was clarified by centrifugation for 1 h at 100,000g (45 Ti rotor, Beckman Coulter). The clarified lysate was incubated with Ni2+-NTA agarose resin for 1 h at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was collected and centrifuged at 100,000 x g for 1 h in an ultracentrifuge (Model XL-90, Beckman Coulter). The supernatant was collected ...
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Proteome of the secondary plastid of Euglena gracilis reveals metabolic quirks and colourful historybioRxiv - Evolutionary Biology 2019Quote: ... The DNase-treated lysate (5 ml) was loaded on top of a sucrose discontinuous density gradient in ultracentrifuge tubes (Cat. No. 344058, Beckman). The gradient was prepared by layering decreasingly dense sucrose solutions upon one another from 2.0 ...
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bioRxiv - Microbiology 2020Quote: ... Whole-cell lysates were then spun at 35,000 rpm for 45 min in a Type 45 Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... lysates were spun for 24 h at 4°C at 50000 rpm in a Type 70 Ti rotor (Beckman Coulter) through a 40 % (w/v ...
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bioRxiv - Genomics 2022Quote: ... Lysates were fractionated on a 10% to 60% sucrose gradient using the SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter) at 36,000 rpm for 3 h and 10 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Automated extraction of gDNA from the lysate was carried out on a Biomek NXp robot (Beckman Coulter, Miami, FL, USA) using the ReliaPrep Large Volume HT gDNA Isolation System (Promega ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were lysed using a sonicator to deliver ~ 8000 J of energy and lysate was clarified at 18000 rpm in a JA-20 rotor (Beckman) for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 25 000 rpm for 45 min at 4°C in a Ti45 rotor (Beckman). Supernatant was incubated with 5 ml of Glutathione Sepharose 4B beads slurry (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by sonication and the lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The lysate was passed three times though 2mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by sonication and the lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The lysate was passed three times through 2 mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 150,000 rcf for 45 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 503,000 rcf for 45 min at 4°C using a Type 70 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... The amount of radioactivity in the cell lysate was measured using a liquid scintillation counter (Beckman Coulter, Brea, CA, USA) and protein concentration was determined via the Lowry method54 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were cleared by centrifugation for 30 min at 4°C at 18,000 rpm (SS-34 rotor; Beckman-Coulter, USA).
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bioRxiv - Biophysics 2023Quote: ... followed by layering the cleared lysate onto 40 mL of 2 M sucrose cushion and ultracentrifugation in Ti-45 rotor (Beckman) at 42,000 rpm for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... Genetic material was pulled down and purified by mixing the lysate in each well with 2.2x volumes of Agencourt RNAClean XP SPRI beads (Beckman Coulter) and washing 3x with 75 μL of 80% ethanol ...
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bioRxiv - Neuroscience 2023Quote: ... the crude lysate was subjected to iodixanol gradient (15%, 25%, 40% and 60%) ultracentrifugation using a Type 70Ti rotor (Beckman) at 490000 × g ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified by centrifugation at 40,000 rpm for 35 min at 4°C (Type 45 Ti Rotor, Beckman Coulter). The resulting supernatant was mixed with 1ml pre-washed IgG Sepharose 6 FastFlow beads (Cytiva ...
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bioRxiv - Immunology 2023Quote: ... Then this clear cell lysate was loaded on top the gradient and ultracentrifugation was done for 5hrs at 133,000g on SW60Ti (Beckman Coulter) rotor to separate each gradient ...
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bioRxiv - Biochemistry 2023Quote: ... by dounce homogenization (20 strokes).The lysate was clarified by centrifugation at 56,000 rpm in a Type 70 Ti rotor (Beckman Coulter) at 4°C for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed by sonication and lysate was cleared by spinning down in a Ti70 at 65K rpm (4ºC) for 30 min (Beckman Coulter). Cleared lysate was briefly incubated (approximately 5 min ...