Labshake search
Citations for Beckman :
451 - 500 of 1322 citations for Plant DNA Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Libraries were purified and adapter dimers removed with Agencourt AMPure XP beads purification using a 1:1 library:beads ratio (Beckman Coulter). ChIP-seq libraries were sequenced on the Illumina NextSeq 500 system with a 20% phiX spike-in (Illumina ...
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bioRxiv - Genetics 2022Quote: ... followed by 72°C for 1 minute followed by purification with 1X Agencourt AMPure XP beads as per the manufacturer’s instructions (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: ... This PCR amplification was followed by another purification using the Agencourt® AMPure® XP beads (#A63881, Beckman Coulter, UK). A ratio of 6.5 of Solid Phase Reversible Immobilisation beads (SPRI ...
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bioRxiv - Genetics 2023Quote: ... Sub-reactions were mixed and purification was performed using 30 μL AMPure XP magnetic beads (Beckman Coulter; cat. no. A63880) according to manufacturer’s instructions and eluted in 21 μL H2O ...
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bioRxiv - Immunology 2023Quote: ... and pellet was resuspended and homogenised for final purification by sucrose cushion separation after ultracentrifugation in an MLS 50 rotor (Beckman). The final purified virus stock was diluted in TNE buffer (20 mM Tris-HCl pH 8 ...
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bioRxiv - Immunology 2024Quote: ... Size-selection and purification of cDNA fragments with preferentially 200 bp in insert length was performed using SPRIBeads (Beckman-Coulter). Size distribution of cDNA libraries was measured using the Agilent high sensitivity DNA assay on a Tapestation 2200 (Agilent) ...
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bioRxiv - Cancer Biology 2023Quote: ... 50-100μl of the pooled library preparation reactions was used to perform magnetic bead-based purification and elimination of any residual free primer using a 0.8X ratio SPRIselect beads (Beckman Coulter) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: DNA preparation: 200 ng genomic DNA was purified using Agencourt AMPure XP Reagent (Beckman Coulter, Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA was sequenced by Beckman Coulter Genomics (Grenoble ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and fragments between 400-500 bp were selected for purification using a 25X AMPure beads solution (Beckman Coulter Inc, Indianapolis, IN). Fragments were then amplified with a NEBNext Ultra Q5 PCR protocol ...
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bioRxiv - Genomics 2020Quote: ... 300ul was transferred to a fresh 1.5-mL Eppendorf tube for two rounds of purification using AMPure XP paramagnetic beads (Beckman Coulter, Inc.) with ratios of 0.5X and 1.3X respectively ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were purified using 1.8X Agencourt AMPure XP PCR Purification beads according to the manufacturer’s instructions (Beckman Coulter, cat no A63881). The sheared DNA samples were quantified and qualified on a BioAnalyzer system using the DNA7500 assay kit (Agilent Technologies cat no ...
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bioRxiv - Genomics 2022Quote: ... 1 µg of whole-genome amplified gDNA was sheared into ∼8 kb fragments followed by purification and size-selection using AMPure XP beads (Beckman Coulter). Oxford Nanopore Technology (ONT ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were purified and adapter dimers removed with Agencourt AMPure XP beads purification using a 1:1 library:beads ratio (Beckman Coulter, #A63880). ChIP-seq libraries were sequenced on the Illumina NextSeq 500 system with a 20% phiX spike-in (Illumina ...
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bioRxiv - Genetics 2022Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase incubated for 4 hours at 37°C followed by inactivation with 1 μL of 10% SDS and purification using RNA Ampure XP beads (Beckman Coulter).
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bioRxiv - Molecular Biology 2022Quote: ... DNA library was purified and size selected using a double-sided bead purification protocol with AMPure XP beads that removes both unwanted small and large fragments (Beckman Coulter). The QuBit dsDNA HS Assay (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... PacBio libraries were constructed using the manufacturer’s protocol and were size selected for 30 kb fragments on the BluePippin system (Sage Science) followed by subsequent purification using AMPure XP beads (Beckman Coulter). The PacBio libraries were sequenced on a PacBio RSII system with P6C4 chemistry ...
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bioRxiv - Plant Biology 2019Quote: ... PacBio libraries were constructed using the manufacturer’s protocol and were size selected for 30 kb fragments on the BluePippen system (Sage Science) followed by subsequent purification using AMPure XP beads (Beckman Coulter). The PacBio libraries were sequenced on a PacBio RSII system with P6C4 chemistry ...
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bioRxiv - Molecular Biology 2021Quote: ... Amplicons corresponding to the expected size (584 bp) were identified by gel electrophoresis before purification with AMPure XP (Beckman Coulter, Australia). To capture any weak detections ...
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bioRxiv - Genomics 2021Quote: ... The nuclease digested DNA (which included both the MN and SN fractions) was directly used for library prep after purification with the Agencourt AMPure XP beads (Beckman Coulter). The MN and SN fractions were separated after sequencing and read mapping using bioinformatics tools as described below ...
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bioRxiv - Genomics 2021Quote: ... A second set of PacBio libraries were constructed using the manufacturer’s protocol and were size selected for 20 kb fragments on the BluePippen system (Sage Science) followed by subsequent purification using AMPure XP beads (Beckman Coulter). Sequencing was performed by the University of Delaware DNA Sequencing & Genotyping Center.
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bioRxiv - Genomics 2020Quote: ... These pooled single-cell libraries were used in an AMpure XP Bead-based Dual Bead Cleanup and Size Selection reaction (Agencourt AMPure XP PCR Purification modified Instructions for Use, Beckman Coulter). In both bead cleanup reactions ...
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bioRxiv - Microbiology 2022Quote: ... The amplified libraries were subjected to a single left-sided bead purification using the AMPure XP magnetic beads (Beckman Coulter, Germany). Libraries were sequenced on SP lane of NovaSeq 6000 System (Illumina ...
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bioRxiv - Physiology 2022Quote: ... cDNA fragments of 150∼200 bp were selected by purification of library fragments using AMPure XP system (Beckman Coulter, Beverly,USA). PCR was performed with Phusion High-Fidelity DNA polymerase ...
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bioRxiv - Genomics 2023Quote: ... and paramagnetic beads (1:1 beads/sample) using the same purification protocol as with AMPure XP beads (Beckman Coulter, no. B37419AB). The product purity was assessed via capillary electrophoresis on a QIAxcel instrument (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... Unique dual-indexed primers were used to amplify each sample followed by purification using Agencourt AMPure XP beads (Beckman Coulter, A63881). The libraries were pooled based on lung weights to ensure even reading depth and sequenced (read length 2×150bp ...
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bioRxiv - Cancer Biology 2023Quote: ... then subjected to PCR amplification and double-sided bead purification (to remove primer dimers and larger than 1,000 bp fragments) using the AMPure XP beads (Beckman Coulter, A63881). Library size distribution and quality was measured using the High Sensitivity DNA Kit (Agilent ...
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bioRxiv - Genomics 2023Quote: ... After a subsequent wash step with ice cold 70 % ethanol the resulting DNA pellet was resuspended with 100 μL PCR-grade water followed by further purification via solid-phase reversible immobilization using 1.5 volume of AMPure XP Beads (Beckman Coultier™) and final elution in 20 μL 1× TE ...
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bioRxiv - Microbiology 2023Quote: ... The remaining PCR reaction of samples treated with a single gRNA was subjected to purification with AMPure XP reagent (Beckman Coulter) according to the manufacturer’s manual using AMPure beads ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products in the two groups were mixed and were purified using the Agencourt AMPure XP PCR purification system (Beckman Coulter).
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Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: ... Samples were purified using 1.8X Agencourt AMPure XP PCR Purification beads according to the manufacturer’s instructions (Beckman Coulter, cat no A63881). The sheared DNA samples were quantified and qualified on a BioAnalyzer system using the DNA7500 assay kit (Agilent Technologies cat no ...
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bioRxiv - Molecular Biology 2024Quote: ... The circles of interest in the sample were then purified through the circle purification step detailed above (section 2.4.) using a 1.8x volume ratio of AMPure XP beads (Beckman Coulter, CA, USA). The efficiency of each sample purification was assessed through a standard qPCR assay using SYBR™ Green PCR Master Mix (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Unique dual-indexed primers were used to amplify each sample followed by purification using Agencourt AMPure XP beads (Beckman Coulter, A63881). The libraries were pooled based on lung weights to ensure even read depth and sequenced (read length 2 × 150 bp ...
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bioRxiv - Cell Biology 2023Quote: ... Excess teltail ss-adaptor and short bulk genomic DNA fragments were removed by DNA size selection using 25 μl SPRI select (Beckman, 0.5x v/v SPRI beads to DNA suspension). After 2 washes with 80% EtOH according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... eluted DNA fragments were purified and subject to size selection of DNA using SPRI beads (Beckman Coulter #B23318) following the manufacturer’s instruction for double size selection with 0.5× and 1.3× bead volume-to-sample volume ratio ...
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bioRxiv - Evolutionary Biology 2021Quote: DNA was used for sequencing by Beckman Coulter Genomics (Grenoble ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted using magnetic beads (Beckman Coulter ...
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bioRxiv - Genetics 2021Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase with addition of the buffer incubated for 60 min at 37 °C followed by inactivation with 1 μL of 10% SDS and purification using RNA clean SPRI beads (Beckman Coulter, A63987).
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bioRxiv - Molecular Biology 2020Quote: ... using overnight incubation as described in the protocol for <0.3kb transcripts.(28) CrRNAs were purified using RNAClean XP magnetic bead purification (Beckman Coulter, Indianapolis, IN) and quantified by NanoDrop spectrophotometry (ThermoFisher ...
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bioRxiv - Molecular Biology 2019Quote: ... were ligated to the DNA fragments followed by a clean-up and a size selection of 500-700 nucleotides long fragments with Agencourt AMPure XP purification beads (Beckman Coulter, USA). Finally ...
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bioRxiv - Genomics 2021Quote: ... we amplified both input and outputs followed by purification and dual size selection using AMPure XP beads (Beckman Coulter, Brea, CA, USA) selecting for fragments between 200 bp–500 bp ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we carried out an additional purification step on the supernatant using 1.8x volume of AMPure XP beads (Beckman Coulter, Brea, CA, USA) and eluted samples in 20 µl 1x TE (Fisher Bioreagents ...
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bioRxiv - Neuroscience 2023Quote: ... Collection of cells and media for AAV harvesting began 72 hours following transfection before iodixanol gradient ultracentrifugation purification using a Type 70 Ti Fixed-Angle Titanium Rotor (Beckman-Coulter, 337922). Titer was calculated using digital droplet PCR (ddPCR ...
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bioRxiv - Molecular Biology 2024Quote: ... the purified product underwent an additional purification step in which 90 μL of AMPure XP beads (1.8x ration) (Beckman Coulter, CA, USA) was added to each sample ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA was enriched in samples with low DNA concentration (1-60 ng/μl) using AMPureXP Beads (SPRIselect, Beckman Coulter) in a 1:1 ratio ...
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bioRxiv - Neuroscience 2020Quote: ... Sequencing products were cleaned up using magnetic beads (CDTR-0050; CleanNA, Waddinxveen, The Netherlands) and according to the Agencourt AMpure PCR purification (Beckman Coulter, Brea, USA) protocol ...
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bioRxiv - Neuroscience 2020Quote: ... to obtain fragments in the size range of 150 bp followed by purification with AMPure XP beads (×1.8v/v) (Beckman Coulter A63881, Indianapolis, IN). Purified DNA was resuspended in 45 μl elution buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... The DNA from the above collected samples was extracted using a thermal lysis protocol along with AMPure XP magnetic beads purification (Beckman Coulter, CA, USA). Samples were processed with fifteen replicates for each bacterial CFU dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... Non-target by-products were removed as required from some 12S PCRs by purification with magnetic Agencourt AMPure beads (Beckman Coulter, Krefeld -Germany).
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bioRxiv - Systems Biology 2019Quote: ... and divided into three 25-μL replicate reactions during both first- and second-step cycling reactions and cleaned after the first and second step using Agencourt AMPure XP-PCR purification (Beckman Coulter, Brea, CA) according to manufacturer instructions ...