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Citations for Beckman :
501 - 550 of 1017 citations for Nipah Virus Glycoprotein G Recombinant Protein Mouse Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... NP-40 prepared in DPBS] and then ultracentrifuged at 100,000x g for 1 h at 4 °C using a TLA-55 rotor (Beckman). The supernatant was removed ...
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bioRxiv - Developmental Biology 2023Quote: ... The cytosolic lysate was layered on 10%-50% sucrose gradients and centrifuged at 246,000 g for 65 min in SW55 swinging bucket rotor (Beckman). Continuous UV absorbance was monitored with Triax flowcell monitor (BioComp).
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bioRxiv - Biochemistry 2023Quote: ... at 18000 PSI and cell debris were separated by centrifugation (4 °C, 30 min, 75,600 ×g, JA25.50 rotor Beckman Coulter). The supernatant was loaded on an affinity chromatography column (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... of proteins were applied on the top of a 16 ml 10-30% continuous sucrose density gradient and centrifuged at 74,400 g for 15 h at 4 °C (SW 32.1 Ti; Beckman Coulter). 24 equal volume fractions were collected ...
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bioRxiv - Molecular Biology 2023Quote: ... Small extracellular vesicles were pelleted at 100,000 X g for 1,5 h at 4°C by using Ti-45 rotor (Beckman Coulter) and 70 ml polycarbonate bottles (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... Pellets were pooled in 1 ml PBS and centrifuged at 100,000 X g for 1,5 h at 4°C in 1,5 ml tubes in a Ti-45 rotor (Beckman Coulter) using adapters (#11004 ...
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bioRxiv - Neuroscience 2023Quote: ... Fractions were washed by diluting with 7 mL of PBS and ultracentrifuged at 100,000 x g (Type 70.1 Ti rotor, Beckman Coulter) for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... the obtained supernatant was filtered through a 0.22 μm filter before ultracentrifugation at 150,000 g for 2 h at 4 °C (Beckman Coulter). The nsEV-containing pellet was resuspended in phosphate buffer saline (PBS).
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bioRxiv - Developmental Biology 2023Quote: ... the supernatant was centrifuged in a TLA100.4 fixed angle rotor at 100000 g for 1h at 37°C in an Optima TL ultracentrifuge (Beckman). The supernatant containing globular actin was collected ...
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bioRxiv - Biochemistry 2023Quote: ... The cell debris was removed by low-speed centrifugation for 30 minutes and the supernatant was further subjected to high-speed centrifugation at 150,000 × g for 120 minutes using a Ti-45 rotor (Beckman). Then the membrane component was resuspended and homogenized using a glass dounce homogenizer for 20 cycles on ice in solubilization buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then centrifuged at 82,600×g at 4°C for 2 h (Beckman ultracentrifuge optima L-100 XP). After the first ultracentrifugation ...
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An adaptive biomolecular condensation response is conserved across environmentally divergent speciesbioRxiv - Cell Biology 2023Quote: ... 500 µL was transferred to a vacuum safe tube spun at 100,000 g for 20 minutes at 4°C (fixed-angle TLA-55 rotor in a Beckman Coulter Optimax tabletop ultracentrifuge) ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was incubated for 30 min at 4 °C and centrifuged at 100,000 × g at 4 °C for 1 h using a TLA100 fixed angle rotor (Beckman). The supernatant fraction was carefully removed ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were spun at 200,000 × g at 4°C for 4 h in an MLS-50 rotor (Beckman Coulter), and 60 µL of top layer was collected ...
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bioRxiv - Plant Biology 2024Quote: ... and centrifuged for 3 h at 260,000 g using an SW 55 Ti swinging-bucket rotor (Beckman Coulter, USA). The final supernatant was discarded ...
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bioRxiv - Neuroscience 2023Quote: ... The tubes were centrifuged at 100,000 × g for 2 hours using a SW 55Ti swinging-bucket rotor (Beckman Coulter).
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bioRxiv - Neuroscience 2024Quote: ... Ultracentrifugation was performed for 2.5 h at 18 °C at 350,000 × g in a type 70 Ti rotor (Beckman Coulter). The interface between the 60 and 40% gradient was extracted along with the 40% layer ...
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bioRxiv - Neuroscience 2024Quote: ... by centrifugation at 5,000 x g at 4°C for 30 minutes in a fixed angle rotor (Beckman Coulter). The qEV column washed with 1x PBS was then loaded with 500 µL of concentrated supernatant followed by 2.5 mL PBS to collect 3 mL void volume ...
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bioRxiv - Molecular Biology 2024Quote: ... for ultracentrifugation at 100,000 × g for 1 hour at 4°C (JXN-30 centrifuge, JA-30.50 Ti Rotor, Beckman Coulter). The pellet was washed with PBS and centrifuged again at 100,000 × g for 1 hour at 4°C to remove soluble contaminant ...
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bioRxiv - Cell Biology 2024Quote: ... The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter). After centrifugation the pellet was resuspended and kept in BRB80T at room temperature.
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was then subjected to ultracentrifugation at 100,000 x g for 2 h at 4 °C to sediment small EVs (Optima L-80K ultracentrifuge, Beckman Coulter ...
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bioRxiv - Biochemistry 2024Quote: ... The radioactive supernatants and dissolved pellets were diluted in ProFlow G+ scintillation liquid for counting (Beckman Coulter LS 6500). The fraction of release was calculated as:
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bioRxiv - Cancer Biology 2024Quote: ... 100 μg/mL cycloheximide) for 2 h at 40,000 g at 4 °C using an SW41 rotor (Beckman Coulter). Gradients were fractionated using Teledyne Isco Tris Peristaltic Pump and fractions were collected and pooled according to the UV trace ...
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bioRxiv - Biochemistry 2023Quote: ... lysates were centrifuged at 60,000 x g (4 °C) for 45 min (Beckman Avanti JXN-26 (rotor: JA 25.5)) and immediately supplemented with 10 mL pre-washed Ni2+-NTA agarose (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 minutes in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 2 ml of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... Cells debris was removed by low-speed centrifugation for 30 minutes and the supernatant were further subjected to high-speed centrifugation at 150,000 g for 90 minutes using a Ti-45 rotor (Beckman), and the membrane component was collected ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 M sucrose) at 100,000 rpm (543,000 × g) and 4°C for 1 h with a TLA110 rotor (Beckman Coulter) and an Optima MAX-TL Ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Microbiology 2020Quote: ... fluorescein isothiocyanate (FITC)-conjugated mouse monoclonal antibody KC57 (Beckman Coulter) was used at a dilution of 1:100.
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bioRxiv - Cell Biology 2023Quote: ... secondary anti-mouse-HRP (Beckman Coulter, Ref. 715-035-151), secondary anti-rabbit-HRP (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... Excess dye was removed by ultracentrifugation of the virus through a 10% sucrose cushion for 2 h at 107,000 × g and 4°C using an SW41 rotor (Beckman Coulter). Labeled virus pellets were resuspended at a viral protein concentration of 1 mg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... 10 U/mL SuperASE-In and was ultracentrifuged at 100,000 × g for for 16 h at 4 °C in a SW Ti41 rotor (Beckman Coulter). After removing the supernatant ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by ultracentrifugation at 30,000 × g 30 min at 4°C using a Ti45 rotor (Beckman Coulter) twice ...
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bioRxiv - Biophysics 2020Quote: ... The insect cells were harvested after 4 days by centrifugation at 300 g for 10 min at 4 °C in an Avanti J-26S ultracentrifuge (JLA-9.1000 rotor, Beckman Coulter). The cell pellet was resuspended in 5 ml ice-cold phosphate buffered saline (PBS ...
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bioRxiv - Biophysics 2020Quote: ... and centrifuged at 45000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter). The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...
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bioRxiv - Cell Biology 2020Quote: ... Crude mitochondria were loaded on top of the chilled gradient and separated at 100,000 Å∼ g for 1 h at 4°C (SW41 rotor; Beckman). Intact purified mitochondria were recovered from a brown band at around 16% Histodenz™ concentration ...
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bioRxiv - Cell Biology 2020Quote: ... and the gradient was centrifuged for 60 min at 24,700 × g at 4 °C using a TLS-55 swinging bucket rotor (Beckman Coulter). Purified mouse POS were resuspended in DMEM/Ham’s F-12 containing 2.5% sucrose ...
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bioRxiv - Microbiology 2020Quote: ... sanguinis was grown as described above and centrifuged for 10 minutes at 3,740 x g in an Allegra X-142 centrifuge at 4°C (Beckman-Coulter). The supernatant was discarded and the cells were resuspended in BHI prior to inoculation ...
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bioRxiv - Biochemistry 2022Quote: ... 2 s off at 30 % amplitude for 10 min and cell debris was removed by centrifugation at 130,000 ×g for 45 min with a Type 70 Ti Rotor (Beckman Coulter). The supernatant was filtered through a 0.22 μm filter (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... columns were spun at 100.000 g for 24 hours using a SW-28.1 rotor (k-factor: 276) in an optima XPN-80 ultracentrifuge (Beckman coulter), and subsequently fractions of 1 mL were collected ...
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bioRxiv - Molecular Biology 2020Quote: ... This supernatant was ultracentrifuged at 100,000×g for 90 min in a SW-28Ti Rotor Swinging Bucket rotor (Beckman Coulter) and resuspended in PBS ...
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bioRxiv - Biophysics 2022Quote: ... The cell suspension was centrifuged for 5 min at 2000 g (Centrifuge Beckman Coulter Allegra X-15-R, Rotor Sx4750A)) and 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Viral bands were pooled and re-precipitated by centrifugation in PBS(-) at 120k × g for 1.5 h in an SW55 rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The insect cells were harvested after 4 days by centrifugation at 300 g for 10 min at 4 °C in an Avanti J-26S ultracentrifuge (JLA-9.1000 rotor, Beckman Coulter). The cell pellet was resuspended in 5 ml ice-cold phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... and centrifuged at 45000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter). The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...
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bioRxiv - Biochemistry 2019Quote: ... The gradients were centrifuged at 285,000 g for 45 min in a swinging-bucket SW-55 rotor using an Optima LE-80K ultracentrifuge (Beckman Coulter). Gradients were then manually segregated into 30 equal fractions of 165 μl from the bottom using a peristaltic pump and analyzed by immunoblotting or bioassay for PrPSc or infectivity ...
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bioRxiv - Cancer Biology 2019Quote: ... Exosomes were depleted from the complete media by ultracentrifugation for 70 minutes at 100,000x g using a Ultracentrifuge (Beckman Coulter) and SW28 swinging-bucket rotor ...
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bioRxiv - Biochemistry 2019Quote: ... Reactions were spun over 15-30% sucrose gradients made in buffer C with appropriate MgCl2 concentrations at 178,000 g (SW-41 rotor, Beckman Coulter) for 3 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... The total bacterial lysate was centrifuged at 48,384 x g at 4 °C for 1 hr (Beckman JA-25.50 rotor), and the supernatant was applied to a 5 mL HisPur Ni-NTA resin (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... by centrifugation at 4,000 x g for 10 minutes at room temperature in a tabletop centrifuge (Beckman Coulter, Pasadena, CA). Plasmid DNA was isolated from each cell pellet using the Purelink®HiPure Plasmid Maxiprep DNA Purification Kit (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... The unbound cyt c was separated from liposome-bound cyt c by ultracentrifugation at 435,000 g for 2 hours at 4 °C in an Optima TLX ultracentrifuge with a TLA-100 rotor (Beckman Coulter). Immediately after the ultracentrifugation ...