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Citations for Beckman :
51 - 100 of 710 citations for Mouse Eukaryotic translation initiation factor 4E binding protein 3 EIF4EBP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the PCR products were cleaned using AMPure XP binding beads (Beckman Coulter Genomic, Indianapolis, IN, USA). The AMPure XP binding beads (Beckman Coulter Genomic ...
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bioRxiv - Pathology 2023Quote: ... the proportion of HIV-1-infected cells was determined by intracellular staining of the viral protein Gag-p24 with a Mouse anti-p24-FITC antibody (clone KC57, Beckman Coulter).
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was centrifuged at 10,500 rpm (∼20,000 × g, k factor = 1,753) for 70 min (SW28 rotor, Beckman). Pelleted lEVs were resuspended in 13.5 ml of PBS (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... and sedimented again at 10,500 rpm (∼20,000 × g, k factor = 1,983) for 70 min (SW40 rotor, Beckman). The supernatant containing sEVs was centrifuged at 25,000 rpm (∼ 110,000 × g ...
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bioRxiv - Developmental Biology 2022Quote: ... and the optical density (OD) was measured at 450 nm with an ELISA reader (Beckman). The concentration of inflammatory factors was calculated according to the manufacturer’s instruction.
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bioRxiv - Cancer Biology 2020Quote: ... 200 μL of 1x binding buffer were added and suspension was analyzed by flow cytometry (Beckman-Coulter, CytoFLEX). Flow cytometry gates were chosen based on untreated controls for each cell line and values were normalized to controls.
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bioRxiv - Molecular Biology 2019Quote: ... Gradients were centrifuged 18-20h at 192,000g in a SW40 rotor (k-factor 144.5) (Beckman Coulter, Brea, CA). For RNA extraction and small RNA library preparation ...
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bioRxiv - Cell Biology 2021Quote: ... 100K EVs were isolated by ultracentrifugation of 10,000xg supernatant at 100,000xg for 65 min in a SW40 rotor (k-factor 280.3) (Beckman-Coulter). EV-containing 100K ultracentrifugation pellets were resuspended in 20 μL PBS containing 0.2% BSA (cleared from aggregates by ultracentrifugation for 16-20 hrs at 100,000xg) ...
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bioRxiv - Biochemistry 2021Quote: ... The library was cleaned up and size-selected by two consecutive rounds of binding to 1.2x and 0.8x SPRI beads (Agentcourt AMPure XP beads, Beckman Coulter). The integrity and size distribution of the final libraries (two biological replicates of KCl-treated and one of untreated ...
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bioRxiv - Genomics 2021Quote: ... Estradiol (E2) and Progesterone assays are competitive binding immunoenzymatic assays performed on the Unicel DXI 800 system (Beckman Coulter). FSH assay is based on Microparticle Enzyme Immunoassay (MEIA ...
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bioRxiv - Microbiology 2022Quote: ... 400 μL of 1X binding buffer was then added before analysing using a CytoFLEX S flow cytometer (Beckman Coutler). Annexin V-FITC positive cells were designated as early apoptotic ...
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bioRxiv - Biochemistry 2023Quote: ... binding reactions were centrifuged at 50,000 RPM for 30 min at 25 °C in a TLA-100 rotor (Beckman). Promptly after centrifugation ...
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bioRxiv - Biochemistry 2023Quote: ... binding reactions were centrifuged at 50,000 RPM for 30 min at 25 °C in a TLA-100 rotor (Beckman). Following centrifugation ...
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bioRxiv - Immunology 2022Quote: ... The occult blood in mouse fecal samples was detected using Hemoccult Test Kit (Beckman Coulter Inc, Fullerton, CA).
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from wild-type mouse livers using the Agencourt RNAdvance Tissue kit (Beckman Coulter, Brea, CA), and cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and the pellet was resuspended in 25 ∝l lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and Red-C12 fluorescence of the starting material ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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bioRxiv - Genomics 2023Quote: Sheared DNA was purified by binding to an equal volume of AMPure XP beads (A63881, Beckman Coulter, Inc. Brea, CA) and eluted in 50µl of 10mM Tris-HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II, respectively); and βHCG and AFP CMIA-ARCHTECT I 4000.The assays were performed as recommended by the manufacturers ...
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bioRxiv - Molecular Biology 2020Quote: ... columns were spun at 100.000 g for 24 hours using a SW-28.1 rotor (k-factor: 276) in an optima XPN-80 ultracentrifuge (Beckman coulter), and subsequently fractions of 1 mL were collected ...
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bioRxiv - Systems Biology 2023Quote: ... The urine was centrifuged at 20,000 x g for 30 minutes at 4°C (k-factor 1,790) in an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW32 Ti swinging bucket rotor (Rmin 67 ...
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bioRxiv - Cell Biology 2020Quote: ... The 1 ml concentrates were overlaid on top of the gradient, and centrifuged at 100 000 g (28 000 rpm, k-factor 266) in an Optima L-90K ultracentrifuge (Beckman Coulter) using an SW41 Ti rotor for 18 h at 4 °C with slow deacceleration ...
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bioRxiv - Biophysics 2022Quote: ... EVs were separated from protein aggregates and free reagents by bottom-up density gradient centrifugation in sucrose for 17.30 h at 192,000 g and 4°C using a SW40 rotor (k-factor 144.5; Beckman Coulter, Fullerton, California, USA). Twelve fractions of 1 mL were then collected from the top of the gradient and respective densities were determined by refractometry using an Atago Illuminator (Japan) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were ultracentrifuged at 180,000 × g (38,000 rpm) in a SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter, k-factor 145) for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Binding reactions were spun at 300,000 g for 30 min at 25 °C using Optima MAX-TL ultracentrifuge (Beckman Coulter, Brea, CA). Bundling reactions were spun at 17,000 g for 15 min at 25 °C ...
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bioRxiv - Bioengineering 2024Quote: ... the stained cells were washed twice to remove any unspecific binding antibodies and were then subjected to measurement using a Beckman Cytoflex S flow cytometer (Beckman, CA, USA). The resulting data were analyzed using FlowJo software (Tree Star ...
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bioRxiv - Immunology 2023Quote: ... The OD was read at 450 nm using an automatic ELISA plate reader (DTX 880, Multimode detector; Beckman Coulter).
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bioRxiv - Immunology 2020Quote: ... KIR2DL2/3/S2 (GL183, PE/Cy5.5, Beckman Coulter,), NKG2C (134591 ...
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bioRxiv - Systems Biology 2023Quote: ... enumerated using a Multisizer 3 Coulter Counter (Beckman,), and then seeded at the desired density in 6-well tissue culture plates (TPP ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with an additional 400 µl 1× Binding Buffer and analyzed within 20 min by CytoFLEX S (Beckman Coulter Life Science). At least 1 × 104 cells were analyzed to determine the percentage of apoptotic cells ...
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bioRxiv - Cancer Biology 2020Quote: ... 320 μL of 1X binding buffer was added to each sample before analysis on the EPICS XL flow cytometer (Beckman Coulter, Buckinghamshire, UK).
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bioRxiv - Biophysics 2022Quote: ... The tubes were filled with PBS to reach the final volume and samples were ultracentrifuged at 120,000 g for 60 minutes at 4°C (70.1 Ti rotor, k-factor 36, Beckman Coulter, Brea, CA, USA). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... Virus was concentrated from cell-free culture supernatant by high-speed ultracentrifugation through a 30% sucrose cushion at 80,000xg for 16 hrs in a SW32 rotor (k-factor 321) (Beckman Coulter, Brea, CA).
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bioRxiv - Neuroscience 2023Quote: ... the samples were concentrated to a final volume of 240 µl through ultracentrifugation at 42,300 rpm using a Beckman TLA-55 rotor (RCFavg: 80,160; RCFmax: 110,220; k-factor: 110 and the Optima MAX-XP ultracentrifuge (both Beckman Coulter, Brea, CA, USA) and equal sample volumes were immediately used for the EV Neuro panel A and B (115±25 µl ...
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bioRxiv - Cell Biology 2024Quote: ... while the supernatant was further centrifuged at 118.000 × g (38,800 rpm) (Type Ti 45 fixed-angle rotor, Beckman Coulter, k-factor 178) for 2.5 hours at 4°C to isolate small EVs that were resuspended in PBS and stored at –80°C.
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bioRxiv - Biochemistry 2020Quote: ... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
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bioRxiv - Molecular Biology 2020Quote: Binding to CD16 was studied by a competitive assay with an anti-CD16-PE/3G8 antibody conjugated to phycoerythrin (Beckman Coulter, Brea, CA, USA) and unlabelled test and control antibodies using Jurkat-CD16 cells.
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bioRxiv - Microbiology 2020Quote: EV-depleted medium was obtained by overnight ultracentrifugation of DMEM supplemented with 20% FBS at 100,000xg in a Type 45 Ti rotor (Beckman Coulter, K-factor 1042.2). After ultracentrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... The gradients were centrifuged at 45,000 rpm for 16 hours at 4 °C using an Optima™ XPN-80 centrifuge with SW60 Ti rotor (average RCF of 207,976 ×g, maximum RCF of 272,840 ×g, κ-factor of 81; Beckman-Coulter, Fullerton, United States). After centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... and centrifuged at 16,500 × g (14,500 rpm) at 4°C for 20 min using a Type Ti 45 fixed-angle rotor (Beckman Coulter, k-factor 1.275) to isolate large EVs ...
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bioRxiv - Immunology 2021Quote: ... CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1 ...
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bioRxiv - Immunology 2020Quote: ... recombinant protein and CD14 antibody (Beckman) were used at 20 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: Proteins were collected by centrifugation (Beckman-Coulter JA-14 rotor ...
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bioRxiv - Cell Biology 2023Quote: ... IgG2a Mouse-PE (A09142, Beckman Coulter), IgG1κ Mouse-AF647 (565571 BD Biosciences) ...
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bioRxiv - Developmental Biology 2020Quote: ... a CytoFLEX 3 laser 13 colour flow cytometer (Beckman Coulter) was used ...
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bioRxiv - Cell Biology 2020Quote: ... Protein aggregates were pelleted by ultracentrifugation of the proteins individually at 80,000 rpm in TLA 100 (Beckman Coulter) for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... recombinant protein and anti-CD14 antibody (Beckman) were used at 20 μg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... 100,000xg for 3 h in an Optima XPN-80 (Beckman Coulter) ultracentrifuge using UltraClear Thinwall tubes ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 °C for 3 h (SW40 Ti rotor, Beckman-Coulter, Germany), the supernatant was discarded ...