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Citations for Beckman :
551 - 600 of 1174 citations for Mouse Anti Staphylococcus Aureus Enterotoxin G Antibody SEG 59 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... and centrifuged at 45000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter). The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...
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bioRxiv - Cell Biology 2020Quote: ... Crude mitochondria were loaded on top of the chilled gradient and separated at 100,000 Å∼ g for 1 h at 4°C (SW41 rotor; Beckman). Intact purified mitochondria were recovered from a brown band at around 16% Histodenz™ concentration ...
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bioRxiv - Cell Biology 2020Quote: ... and the gradient was centrifuged for 60 min at 24,700 × g at 4 °C using a TLS-55 swinging bucket rotor (Beckman Coulter). Purified mouse POS were resuspended in DMEM/Ham’s F-12 containing 2.5% sucrose ...
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bioRxiv - Microbiology 2020Quote: ... sanguinis was grown as described above and centrifuged for 10 minutes at 3,740 x g in an Allegra X-142 centrifuge at 4°C (Beckman-Coulter). The supernatant was discarded and the cells were resuspended in BHI prior to inoculation ...
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bioRxiv - Biochemistry 2022Quote: ... 2 s off at 30 % amplitude for 10 min and cell debris was removed by centrifugation at 130,000 ×g for 45 min with a Type 70 Ti Rotor (Beckman Coulter). The supernatant was filtered through a 0.22 μm filter (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... columns were spun at 100.000 g for 24 hours using a SW-28.1 rotor (k-factor: 276) in an optima XPN-80 ultracentrifuge (Beckman coulter), and subsequently fractions of 1 mL were collected ...
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bioRxiv - Molecular Biology 2020Quote: ... This supernatant was ultracentrifuged at 100,000×g for 90 min in a SW-28Ti Rotor Swinging Bucket rotor (Beckman Coulter) and resuspended in PBS ...
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bioRxiv - Biophysics 2022Quote: ... The cell suspension was centrifuged for 5 min at 2000 g (Centrifuge Beckman Coulter Allegra X-15-R, Rotor Sx4750A)) and 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Viral bands were pooled and re-precipitated by centrifugation in PBS(-) at 120k × g for 1.5 h in an SW55 rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The insect cells were harvested after 4 days by centrifugation at 300 g for 10 min at 4 °C in an Avanti J-26S ultracentrifuge (JLA-9.1000 rotor, Beckman Coulter). The cell pellet was resuspended in 5 ml ice-cold phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... and centrifuged at 45000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter). The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...
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bioRxiv - Biochemistry 2019Quote: ... The gradients were centrifuged at 285,000 g for 45 min in a swinging-bucket SW-55 rotor using an Optima LE-80K ultracentrifuge (Beckman Coulter). Gradients were then manually segregated into 30 equal fractions of 165 μl from the bottom using a peristaltic pump and analyzed by immunoblotting or bioassay for PrPSc or infectivity ...
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bioRxiv - Cancer Biology 2019Quote: ... Exosomes were depleted from the complete media by ultracentrifugation for 70 minutes at 100,000x g using a Ultracentrifuge (Beckman Coulter) and SW28 swinging-bucket rotor ...
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bioRxiv - Biochemistry 2019Quote: ... Reactions were spun over 15-30% sucrose gradients made in buffer C with appropriate MgCl2 concentrations at 178,000 g (SW-41 rotor, Beckman Coulter) for 3 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... The total bacterial lysate was centrifuged at 48,384 x g at 4 °C for 1 hr (Beckman JA-25.50 rotor), and the supernatant was applied to a 5 mL HisPur Ni-NTA resin (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... by centrifugation at 4,000 x g for 10 minutes at room temperature in a tabletop centrifuge (Beckman Coulter, Pasadena, CA). Plasmid DNA was isolated from each cell pellet using the Purelink®HiPure Plasmid Maxiprep DNA Purification Kit (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... The unbound cyt c was separated from liposome-bound cyt c by ultracentrifugation at 435,000 g for 2 hours at 4 °C in an Optima TLX ultracentrifuge with a TLA-100 rotor (Beckman Coulter). Immediately after the ultracentrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... actins were cleared by highspeed centrifugation at 316,613 x g for 1 hour at 4°C in a TLA100 rotor (Beckman Coulter). Only the top 80% of the supernatant was collected to perform TIRF microscopy ...
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bioRxiv - Biophysics 2021Quote: ... The polymerized microtubules were diluted in BRB80T (BRB80 supplemented with 10μM taxol (paclitaxel)) and centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter). After centrifugation the pellet was resuspended in BRB80T.
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bioRxiv - Plant Biology 2020Quote: ... The sucrose gradient was centrifuged at 154,300 × g for 15 h at 4 °C using a SW 41 Ti rotor (Beckman Coulter). The LHCII proteoliposomes separated from empty liposomes were collected dropwise from the bottom of the tube and used for analysis.
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bioRxiv - Cancer Biology 2021Quote: ... Tubes were then spun at 10,000 x g for 20 minutes at 4 °C in an ultracentrifuge (Beckman, L8-70M). The supernatant was filtered using 0.2 μM syringe filters (Millipore Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... The pellet was washed with PBS and ultracentrifuged at 100,000 × g 1h at 4°C to obtain small vesicles (TL-100 Ultracentrifuge, Beckman Coulter). The final pellet was resuspended in PBS for subsequent experiments ...
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bioRxiv - Cell Biology 2021Quote: ... The cell lysate was clarified via centrifugation at 66,000 x g for 30 min in a Type 70 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: Exosomes were depleted from the complete media by ultracentrifugation for 70 minutes at 100,000x g using an ultracentrifuge (Beckman Coulter) and SW28 swinging-bucket rotor ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... 700 μl of samples were loaded on the top of the gradients and separated at 38,000 ×g for 14 hrs in a SW41Ti rotor (Beckman Coulter). After the gradients were fractioned into 24 0.5 ml aliquots ...
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bioRxiv - Biochemistry 2021Quote: ... Equal parts of 30% and 10% iodixanol gradient solutions were layered on the mitochondrial fraction and then samples were centrifuged at 50,000 x g for 2 h (Beckman SW41TI). The mitochondrial layer was collected ...
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bioRxiv - Biochemistry 2020Quote: ... 20% FBS containing RPMI was ultracentrifuged for 18□hours at 100,000□g at 4□°C in a 45Ti fixed angle rotor using polycarbonate tubes (both from Beckman Coulter). After ultracentrifugation ...
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bioRxiv - Synthetic Biology 2021Quote: ... The collected supernatants were centrifuged at 300,000 x g for 2 hr with Optima MAX-XP ultracentrifuge with SW 55 Ti Swinging-Bucket Rotor (Beckman Coulter). The pellets consisting of the cell membrane fractions were resuspended into 10 mL of the same glycan containing media and incubated in the anaerobic chamber at 37°C for 16 hr ...
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bioRxiv - Microbiology 2020Quote: ... Phases were separated by centrifugation for 15 min at 4500 g and 6.5 ml of the aqueous phase was transferred to an ultracentrifugation tube (Ultra-Clear, open-top; Beckman coulter) filled with a 2 ml cushion of 5.7 M CsCl in 25 mM sodium citrate ...
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bioRxiv - Plant Biology 2021Quote: ... The plant debris was removed by centrifuging at 16 000 g for 15 minutes at 4°C in a JA-25.50 rotor and Avanti J- 20 XP centrifuge (Beckman Coulter). The clear supernatant was then gently poured on top of 8 ml sucrose cushion (100 mM Tris·HCl (pH 8.0) ...
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bioRxiv - Microbiology 2021Quote: ... bacteria were harvested by centrifugation at 4000 x g for 15 min (Microfuge 20R; Beckman Coulter, La Brea, CA, USA). The pellet was washed twice in PBS ...
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bioRxiv - Microbiology 2021Quote: ... The density gradient centrifugation was performed at 141,000 g and 4 °C for 20 h in a SW28 swing rotor (Beckman Coulter). A total of 18 fractions were collected starting from the top.
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bioRxiv - Microbiology 2021Quote: ... Archaella were pelleted from the supernatant by ultracentrifugation (60,000 × g, 90 min, 4 °C; Beckman Optima LE-80K, rotor 70Ti), resuspended in 150 μl 0.1 M HEPES/NAOH pH 7.0 and purified for 48 h (250,000 × g ...
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bioRxiv - Molecular Biology 2020Quote: ... The sucrose gradients were centrifuged at 200,000 x g avg at 4°C for 173 min using a SW40Ti rotor (Beckman Coulter). After the spin ...
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bioRxiv - Microbiology 2020Quote: ... At the end of each selection cells were pelleted from the media by centrifugation 200 x g for 5 min in an Allegra centrifuge (Beckman) and supernatant was transferred for storage at −80C ...
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bioRxiv - Microbiology 2020Quote: MT-2 cells were collected and washed three times by pelleting at 200 x g for 5 min at 4°C in an Allegra centrifuge (Beckman) and resuspending cells in ice-cold PBS ...
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bioRxiv - Microbiology 2020Quote: ... Virus was pelleted at 1847 x g for 30 min at 4°C in the plate in an Allegra centrifuge (Beckman) and the pellet was resuspended in 50 uL of loading buffer with DTT for analysis by WB with αGag ...
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kinesin recruitment by adapter SKIP on melanosomes is dynamically controlled by LC3B phosphorylationbioRxiv - Cell Biology 2021Quote: ... The post nuclear supernatant was collected and separated on a stepwise sucrose density gradient and centrifuged at 100,000 g for 1 hour at 4°C in a swing-out rotor (Beckman coulter). The sucrose density gradients comprised 0.25 ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... FCS was diluted 1:1 with RPMI and centrifuged at 100,000 g at 4 °C in a swinging-bucket rotor (SW28 or 32, Beckman Coulter) for 18 h ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was then overlaid onto a 2 ml 60% iodixanol cushion and centrifuged at 100,000 × g (SW32 rotor, Beckman Coulter) for 18h ...
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bioRxiv - Cell Biology 2021Quote: ... The post-nuclear supernatants were ultracentrifuged using SW41Ti rotor at 100,000 g for 3 h at 4 °C on Optima L-90K (Beckman Coulter). The fraction between 0.4–0.5 M sucrose was collected ...
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bioRxiv - Cell Biology 2020Quote: ... cells and larger debris were removed by 2000 × g centrifugation of cell suspensions for 15 minutes (Rotor: JS-5.3; Beckman Coulter). MSC-free CM were stored at −20°C until usage ...
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bioRxiv - Biophysics 2022Quote: ... the homogenized solution was brought to 55 mL with more Percoll buffer and centrifuged at 48400 g for 30 minutes in a 45 Ti rotor (Beckman). Chromosomes appeared as a faint band about 1/5th from the bottom of the centrifuge tube ...
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bioRxiv - Biochemistry 2022Quote: ... 40 μl of the mixture was transferred to an ultracentrifuge tube and centrifuged at around 100,000 x g for 15 minutes at room temperature using the Airfuge (Beckman Coulter). After centrifugation ...
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bioRxiv - Microbiology 2022Quote: ... The capsid-host protein complexes were sedimented through a 30% sucrose cushion at 110,000 g for 20 min at 4°C (TLA-100, Beckman-Coulter), resuspended in CBB by ultrasound tip sonication at 40 W for about 5 x 5 seconds on ice ...
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bioRxiv - Microbiology 2022Quote: ... and C capsids were separated by sedimentation at 50,000 x g and 4°C for 80 min (SW40Ti, Beckman Coulter) on linear 20 to 50% sucrose gradients in TKE buffer (20 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... and the debris and membranes were removed by ultracentrifugation at 205,000 g for 1 hr at 4 °C (rotor 45 Ti, Beckman Coulter). The supernatants were applied to IMAC with Ni2+-NTA-agarose resin (Qiagen ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were centrifuged at 41399 g and 6 °C and for 1 hour (Beckman Avanti J-25 centrifuge) to obtain the cytoplasmic protein extract.
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bioRxiv - Cell Biology 2022Quote: ... a range of concentrations of Crn1 protein variants was incubated in F-buffer with pre-polymerized actin filaments at room temperature for 30 min and then spun at 100,000 × g (high speed) with TLA100 rotor (Beckman Coulter) for 20 min at 25°C ...
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bioRxiv - Neuroscience 2020Quote: ... The V and VI fractions were diluted to a total volume of 12 mL with dfPBS and centrifuged at 140,000 × g for 70 min at 4°C (# Optima-XE SW41 Beckman Coulter), and each pellet were resuspend with 30 μl of dfPBS ...