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Citations for Beckman :
151 - 200 of 479 citations for Mouse Anti Human Papilloma virus type 11 711 13 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 4 ng of RNA were retrotranscribed and cDNA was amplified using 13 cycles and purified with AMPure XP beads (Beckman Coulter). Concentration was determined using Qubit 3.0 (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Pellets were solubilised in 150 μL of cold TNE buffer and a volume of 1.5 ml was laid on a five step 20 - 60% (w/v) sucrose gradient prepared in Ultra-Clear 13 ml tubes (Beckman Coulter). The tubes were centrifuged at 35,000 rpm for 2h at 4°C in a Beckman SW41 rotor ...
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bioRxiv - Molecular Biology 2022Quote: ... The gradients were prepared using freeze-thaw method42 in Thinwall Ultra-Clear ultracentrifuge tubes (5 ml, 13×51 mm; Beckman-Coulter). To create the 50% sucrose cushion ...
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bioRxiv - Microbiology 2023Quote: ... The clarified supernatant was loaded over a 20% sucrose cushion in PBS using Ultra Clear tubes (13 x51 mm, cat n° 344057, Beckman Coulter) in a ratio of 3 to 4 of cushion and sample ...
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bioRxiv - Cell Biology 2023Quote: ... 30% Percoll (v/v)] followed by 3 mL of MRB in a 13 mL ultracentrifuge tube (Beckman Instruments, Inc, CA 331372) and spun at 95,000 x g for 45 min in a TH641 rotor ...
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bioRxiv - Molecular Biology 2023Quote: ... Digested lysates were then overlaid on 900 uL of a 1M sucrose cushion in a 13 x 51 mm polycarbonate tube (Beckman Coulter), followed by ultracentrifugation at 70k RPM at 4⁰C for 2 h (Optima TLX ultracentrifuge ...
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bioRxiv - Cell Biology 2022Quote: ... the virus-cell mixture was centrifuged at 1,000 rcf for 2 hours at 32°C (Beckman Coulter, Allegra X-30R). Infected cells were then cultured as described above and subsequently FACS-sorted for the establishment of clonal cell lines.
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bioRxiv - Biophysics 2023Quote: ... Virus-containing supernatants were collected at 48 hours and 72 hours after transfection and concentrated using ultracentrifugation (Ultraclear, Beckman 344058). The transduction capacity of the viral vector product was confirmed by culturing non-transfected HEK293T cells with the virus after 24 hours and assessing via fluorescent imaging.
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bioRxiv - Cell Biology 2022Quote: ... labeled filamentous actin was centrifuged at 167,000 x g (Type 50.2 rotor, Beckman Coulter) for 3 hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was clarified by centrifugation in a Type 45 Ti rotor (Beckman Coulter) at 35,000 RPM for 35 min at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... followed by centrifugation at 350,000 xg using a Type 70 Ti ultracentrifuge rotor (Beckman). Following centrifugation ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were cleared by centrifugation at 35000 rpm (Beckman Instruments type 45 Ti rotor) for 1.5 h at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were cleared by centrifugation at 35000 rpm (Beckman Instruments type 45 Ti rotor) for 1.5h at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... fixed angle Type 45 Ti rotor (k factor = 133; Beckman Coulter, Miami, FL, USA), at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... membranes were pelleted by ultracentrifugation for 1.5 h (125,000 g, Beckman Type 45 Ti). The membrane pellet was resuspended in ∼10 pellet volumes of lysis buffer supplemented with 5 μM cotransin ...
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bioRxiv - Biophysics 2023Quote: ... 2 mM DTT) with 0.7 M sucrose in Type 45 Ti tubes (Beckman-Coulter). Ribosomes were pelleted by centrifugation at 27,000 rpm (57,000 xg ...
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bioRxiv - Biophysics 2023Quote: ... and at 4 °C for 1 hour for ultracentrifugation (70,658 g, Beckman, Type 70Ti) in vacuum ...
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bioRxiv - Neuroscience 2023Quote: ... Diluted pellets were ultracentrifuged at 100,000 x g (Type 70.1 Ti rotor, Beckman Coulter) for 2 h using 16 ξ 76 mm polycarbonate tubes (Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... for 1 hour at 4°C in a Type 45 Ti rotor (Beckman Coulter). The clarified lysate was then incubated with 5 mL of Ni-NTA agarose beads (Qiagen) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was centrifuged at 35,000 rpm in a Type 45 Ti rotor (Beckman) for 1 hr at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was centrifuged at 45,000 rpm in a Type 45 Ti rotor (Beckman) for 1 hr at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... starting with 50% and topping off with 15% for a total of 11 mL was added to thin wall polypropylene ultracentrifuge tubes (Beckman Coulter). The tubes were immediately frozen and stored at -80 °C ...
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bioRxiv - Systems Biology 2020Quote: ... each resultant fraction underwent separate ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778). In EV-enriched pooled fraction experiments ...
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bioRxiv - Cell Biology 2023Quote: ... A volume of lysate containing 125 ug RNA was loaded on 11 ml 15-50% sucrose gradients in 14 x 89 mm centrifuge tubes (Beckman Coulter). (Gradients were prepared in 20 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter; 1:100 dilution). The percentage of transfected or infected cells (p24+ ...
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bioRxiv - Bioengineering 2021Quote: The following antibodies were used for flow cytometry staining in a 96-well format: PE-labelled anti-human CD4 (Beckman Coulter, #A07752), APC-labelled anti-human CD8 (Beckman Coulter ...
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bioRxiv - Plant Biology 2020Quote: ... All samples were centrifuged in a single 2 h run with sedimentation at 50,000 g and 4°C using small 13 x 51 mm ultracentrifugation tubes in SW55 Ti rotor (Beckman Coulter, Krefeld, Germany). These centrifugation settings were optimized for polysome and monosome separations ...
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bioRxiv - Neuroscience 2022Quote: ... which was then sealed and placed into a hematocrit spinner (2 minutes at 13, 700 g; StatSpin CritSpin Microhematocrit Centrifuge, Beckman Coulter, Inc). Measurements were taken to calculate percent (% ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell suspensions were diluted 250 μL into 10 mL of ISOTON diluent and cell size was measured on a Coulter Z2 Cell Analyzer (Cat# 844 80 11, Beckman, Indianapolis, IN). Median cell volume was used for calculations ...
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bioRxiv - Microbiology 2020Quote: ... were further purified by centrifugation through a 1.2 g/cm3 to 1.6 g/cm3 cesium chloride density gradient at 50,000 RPM for 4 hours at 11 °C in a Beckman SW 65 Ti rotor (Beckman Coulter, Brea, California). Visible “virus bands” in the gradients were collected using a Hamilton syringe and diluted 1:10 in 10 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... Diluted virions were then centrifuged at 75,000 RPM for 30 minutes at 11 °C in a Beckman TLA 120 rotor (Beckman Coulter, Brea, California). The pellet was resuspended in 10 mM Tris-HCl ...
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bioRxiv - Genetics 2019Quote: ... and ultra-centrifuged for 1.5 hours at 35,000 rpm (TYPE 45 Ti rotor of Beckman) at 4 °C to concentrate the virus ...
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bioRxiv - Cell Biology 2022Quote: ... The solution was then centrifuged at 14,000 x g (Type 45 Ti rotor, Beckman Coulter) for 3 hours at 4°C to collect the filamentous actin pellet ...
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bioRxiv - Molecular Biology 2022Quote: ... the lysate was spun at 30,000 rpm (104,000 × g) in a Type 45Ti rotor (Beckman) for 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was collected and virus pelleted through 30% (w/v) sucrose cushion at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 hours at 10°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Tie2-PE and CD34-FITC antibodies (eBioscience 17-5821-81, 17-1171, 12-5987, 25-0411, 11-0341-82) by flow cytometry (Beckman Coulter CyAn ADP) or confocal microscopy using Confocal Microscope Zeiss LSM 880 with Airyscan.
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bioRxiv - Microbiology 2022Quote: Whole-cell absorbance spectra from 550 to 800 nm were taken from cells grown in BG-11 under VL and FRL for 30 days using a DU 640 spectrophotometer and associated software (Beckman Coulter, Brea, CA). For low-temperature fluorescence measurements at 77 K ...
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bioRxiv - Molecular Biology 2020Quote: ... bound antibodies were visualized using 100 µL of a 1:100 dilution of goat F(ab’)2 anti-human IgG coupled to phycoerythrin (Beckman Coulter, Brea, CA, USA) at 4°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Unsieved soil samples were suspended in water and analyzed on a LS 13 320 Series Laser Diffraction Particle Size Analyzer (Beckman Coulter Brea CA 92821). Particle sizes were grouped according USDA soil texture classification ...
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bioRxiv - Biophysics 2021Quote: ... The crude lysate was centrifuged at 55,000 rpm in a Type 70 Ti rotor (Beckman Coulter) for 1 hour then filtered using 0.22 μm Millex-GP PES membrane filters (Millipore SLGP033RS) ...
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bioRxiv - Cell Biology 2020Quote: ... Both subfractions were centrifuged in a Ti50 rotor at 225,000g for 1h (Beckman Type 70.1Ti rotor) at 4C ...
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bioRxiv - Cell Biology 2020Quote: ... Lysate was clarified at 50K for 30 min in a TLA 100.3 type rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... The crude lysate was centrifuged at 55,000 rpm in a Type 70 Ti rotor (Beckman Coulter) for 1 hour then filtered using 0.22 μm Millex-GP PES membrane filters (Millipore SLGP033RS) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was cleared by centrifugation at 40,000 rpm (type 45 Ti rotor; Beckman, Brea, CA) for 1 hour at 4°C and then was loaded onto Pierce™ Glutathione Agarose (16101 ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifuging at 66,000g for 30min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 2mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... The lysate was cleared by centrifugation at 40,000 rpm (type 45 Ti rotor; Beckman, Brea, CA) for 1 hour at 4°C and then was loaded onto Pierce™ Glutathione Agarose (16101 ...
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bioRxiv - Systems Biology 2022Quote: ... supernatant containing metabolites extracted from 1.1×109 cells and 11 spike-ins were aliquoted into 1,000 vials using an automated liquid handler (Biomek 4000, Beckman Coulter, Inc., Brea, California, USA), with 5 μL solution per tube ...
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bioRxiv - Microbiology 2021Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution) in combination with Nef or Vpu rabbit antisera (1:1000 dilution) ...
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bioRxiv - Microbiology 2023Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA, 1:100 dilution). Flow cytometry measurements were performed using a FACSCalibur (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... After two short clearance spins (Type 45 Ti rotor from Beckman Coulter, 15 min at 20000 rpm), lysates were spun for 24 h at 4°C at 50000 rpm in a Type 70 Ti rotor (Beckman Coulter ...