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Citations for Beckman :
351 - 400 of 514 citations for Mouse Anti Herpes Simplex Virus 2 gD 0192 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... filtered supernatant was underlaid with 20% sucrose PBS using a pipetting needle and centrifuged at 39 000 rpm for 2 h using an SW-41 rotor (Beckman Coulter). The supernatant and sucrose solution were carefully removed ...
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bioRxiv - Microbiology 2022Quote: ... The virus suspension was pelleted through a 40% sucrose cushion by centrifugation for 2 h at 40,000 rpm in a SW55 Ti rotor (Beckman, Fullerton, CA) at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 2 h at 16°C and double stranded cDNA was purified using AMPure XP beads (Beckman Coulter). For testing transcription activity of predicted genes ...
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bioRxiv - Cancer Biology 2019Quote: ... Gradients were ultra-centrifuged at 221,600 × g for 2 hours at 4°C (SW-41Ti rotor, 331362, Beckman Coulter, Brea, CA). Gradients were fractionated (Piston Fractionator ...
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bioRxiv - Genomics 2019Quote: ... Cleared cell extracts were ultracentifuged at 34400 rpm for 2 hours 40 minutes at 4C using a C-1000 XP centrifuge with SW40 rotor (Beckman Coulter). Gradient UV absorption at 254 nm was measured and selected fractions were selected for 5’cap library preparation (5µg purigied RNA per sample) ...
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bioRxiv - Developmental Biology 2019Quote: Detection of apoptotic TCam-2 cells was performed 48 h after transfection of TCam-2 cells with control and KIF18A siRNA constructs using an Annexin V-FITC Apoptosis Detection Kit (Beckman Coulter), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... to remove cellular debris and purified by ultracentrifugation over a 25% sucrose cushion (28.000 rpm for 2 hours; Beckman Coulter ultracentrifuge).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were treated with 3 different doses of the conventional chemotherapy for 2 days and cell viability was assessed using a Vi-cell XR Viability Analyzer (Beckman Coulter); 6 replicates were performed for each cell line and each dose.
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bioRxiv - Microbiology 2019Quote: ... PCR products were verified with a 2% agarose gel electrophoresis and purified using AMPure XP bead (Beckman Coulter, Brea, CA, USA). The purified amplicon library was quantified and sequenced on the MiSeq Platform (Illumina ...
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bioRxiv - Plant Biology 2019Quote: ... for 16 h before the radiolabel was quantified by scintillation for 2 min using a LS6500 Multi-Purpose Scintillation Counter (Beckman Coulter). CPM values were scaled to the average for the 8 week long day plant sample at 6-9 mm or 8 week long day total samples.
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bioRxiv - Genetics 2020Quote: ... Timepoints were measured every 2-3 days by flow cytometry analysis of >7,000 cells (either a BD Accuri C6 or Beckman Coulter CytoFLEX). Data was analyzed using Cytoflow (https://github.com/bpteague/cytoflow ...
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bioRxiv - Genetics 2020Quote: ... Reverse crosslinking reactions were incubated 2 h at 65°C and cleaned using Agencourt Ampure XP SPRI beads (Cat #A63880, Beckman Coulter) with a 2x bead:sample ratio ...
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bioRxiv - Immunology 2021Quote: ... Beckman Coulter Access SARS-CoV-2 IgG assays (Brea, CA, USA) targeting the RBD were performed on UniCel DxI 800 Access Immunoassay System (Beckman Coulter). Index value threshold for positivity was 1 as recommended ...
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bioRxiv - Immunology 2020Quote: ... erythrocytes in 2 μl blood (collected from the tail tip) were counted using a Z2 Coulter Particle count and size analyser (Beckman Coulter). We determined the healthy range in uninfected C57Bl/6J mice housed in our facility to be 8.8 - 10.5*109 erythrocytes*ml−1 ...
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bioRxiv - Microbiology 2021Quote: ... and either aliquoted or concentrated by ultracentrifugation using 20% (w/v) sucrose cushions for 2 hours at 25,000 rpm in an SW41 or SW28 rotor (Beckman, Indianapolis, IN). Alternatively ...
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bioRxiv - Immunology 2020Quote: ... 2) first-round PCRs were set up using a Biomek FX liquid handling system with a 96- well head (Beckman Coulter); 3 ...
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bioRxiv - Immunology 2021Quote: ... Samples were spun at 76,650 x g for 2 hours at 4° C inside a SW 32i rotor using an Optima L-90K Ultracentrifuge (Beckman Coulter). Purified virus was quantified using a bicinchoninic acid assay (BCA ...
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bioRxiv - Genomics 2021Quote: ... samples were purified with 45 µl of Agencourt AMPure XP magnetic beads with a sample to beads ratio of 3:2 (Beckman Coulter), normalized by Qubit quantification and pooled to generate a 4 nM library ...
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bioRxiv - Genomics 2021Quote: ... The samples were then centrifuged at 28,600 rpm for 2 h at 4°C (Beckman Optima XE-90; SW32 Ti rotor). After centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Vesicles recovered by ultracentrifugation of filtered supernatant at 150,000 x g for 2 h at 4 °C using a Ti 45 rotor (Beckman Instruments, USA), were washed with phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2022Quote: ... A 12 ml 2 % step Optiprep gradient in LB ranging from 32 % to 10 % was prepared beforehand in Ultra-Clear tubes (Beckman Coulter). Samples were spun for 16 hours at 100000 g at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... layers F1/2 were removed before transferring the combined F3/4 layer into a clean 25mL centrifuge tube (Beckman Coulter, #355618) and diluting in GM buffer (at least 1:4 ...
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bioRxiv - Microbiology 2023Quote: ... viruses were collected and concentrated by ultracentrifugation (37,000 rpm, 2 h 30, 15 °C; Beckman Type 45 Ti fixed-angle rotor). The virus titer was determined using a plaque assay.
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bioRxiv - Microbiology 2023Quote: ... the DNA monitored on 2% agarose gel was purified by means of AMPure XT beads (Beckman Coulter Genomics, Danvers, MA, USA) and quantified by Qubit (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was filtered with a membrane pore size of 0.2 μm (Whatman) and centrifuged at 120,000 × g for 4 h at 4 °C in polycarbonate bottle assemblies (Beckman coulter, 355603) in a 70.1 Ti fixed angle rotor (Beckman coulter ...
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bioRxiv - Biophysics 2023Quote: ... and 12-fold diluted in ice-cold SCL and subjected to ultracentrifugation at 100 000 x gav for 2 h in a Ti 70 fixed-angle rotor (Beckman Coulter). The resulting pellets were resuspended in SCL and freshly used for downstream analysis and never subjected to freezing and thawing.
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were balanced and subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
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A laboratory framework for ongoing optimisation of amplification based genomic surveillance programsbioRxiv - Genomics 2023Quote: ... PCR amplicons for pools 1 and 2 were combined for each sample and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30μL of sterile water ...
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bioRxiv - Genomics 2024Quote: ... The crude nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI) and loaded into a flow cytometer (Beckman Coulter, MoFlo XDP). Nuclei were purified by flow sorting and washed as described previously [61] ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tube was balanced and subjected to ultracentrifugation at 150,000 × g for 2 h at 4 °C using a swinging-bucket rotor (SW 41 Ti Beckman Coulter, USA).
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bioRxiv - Neuroscience 2023Quote: ... After PCR the samples were combined into small pool of 2-6 samples and size selection was carried out by adding 0.55x volume of AMPure XP beads (Beckman Coulter, #A68831). The concentrations of purified library pools were quantified with Collibri™ Library Quantification Kit (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... 50 mM Tris-HCl pH 7.4) and ultracentrifuged at 250 000 x g for 2 h (Beckman Optima TL 100 Ultracentrifuge). After ultracentrifugation ...
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bioRxiv - Microbiology 2024Quote: Intracellular HIV-1 p24 staining was performed on fixed U1 and ACH-2 cells with KC57-FITC (Beckman Coulter, Cat#6604665). After allowing at least 30 minutes for fixation ...
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bioRxiv - Cell Biology 2020Quote: ... APC-A750 anti-human CD90 (Thy-1/310, #B36121 Beckman Coulter) and Vioblue anti-human CD45 antibodies (REA747 ...
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bioRxiv - Immunology 2022Quote: ... and finally intracellularly stained with anti-p24-PE (KC57, Beckman Coulter) as previously described.
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bioRxiv - Immunology 2020Quote: ... and staining intracellularly with anti-HIV-Gag Kc57-RD1 (Beckman Coulter), and analyzed on a BD LSRFortessa™ flow cytometer ...
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bioRxiv - Immunology 2020Quote: ... 10 μl of Krome Orange anti-CD45 (Beckman Coulter, clone J33), APC-A750 anti-CD3 (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... and soluble anti-CD28 ((1ug/mL; Immunotech/Beckman coulter REF # IM1376) antibodies in serum-free X-Vivo 20 medium (Lonza) ...
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bioRxiv - Immunology 2020Quote: ... Cells were further stained with an anti-CD45RO antibody (Beckman Coulter).
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bioRxiv - Immunology 2020Quote: ... anti-CD45RO ECD (clone UCHL1, Beckman Coulter, Cat. M2712U, 1:100), anti-PD1 APC (clone NAT105 ...
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bioRxiv - Immunology 2022Quote: ... anti-NKG2A-APC (clone Z199; 5 μL; cat. # A60797) from Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... and soluble anti-CD28 (500 ng/ml; Beckman Coulter, REF# IM1376) antibodies in presence of cytokines i.e. ...
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bioRxiv - Microbiology 2023Quote: ... or anti-p24 (Beckman Coulter 6604667, 1:250 in 0.1% Saponin) for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... AlexaFluor 700 anti-human CD38 (clone LS198-4-3, Beckman Coulter), APC-Cy7 anti-human CD19 (clone SJ25C1 ...
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bioRxiv - Bioengineering 2024Quote: ... and anti-CD81-APC (catalog no. A87789) was purchased from Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... The pelleted exosomes from above supernatants were further centrifuged at 100,000g (26,450rpm) for 2 h at 4 °C (Beckman Coulter, Optima XE-90) and resuspended in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Samples were centrifuged at 130,000 × g for 2 h at 4°C in a TLA100.3 rotor (Beckman Optima TLX table top centrifuge). Following centrifugation ...
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bioRxiv - Neuroscience 2021Quote: ... The post nuclear supernatant was transferred to an ultracentrifuge tube and centrifuged for 2 h at 245,000 g at 4 °C in a Beckman L90 Ultracentrifuge (Beckman Coulter, Mississauga, Canada) to pellet remaining membrane.
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bioRxiv - Neuroscience 2020Quote: ... Final 15 mL of solution was mixed by inverting the tube 10x and carefully pipetted into 2 ultracentrifuge tubes (Beckman Coulter #344059) onto the isolation buffer cushion (5 mL ...