Labshake search
Citations for Beckman :
401 - 450 of 664 citations for Methylphosphonic Acid 13C 99%; Methyl D3 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... About 9 ml interface that came from 3×SW28 tubes (25×89 mm, Beckman Coulter) was loaded in a SW41 tube (14×89 mm ...
-
bioRxiv - Cell Biology 2020Quote: ... 40 μL of fixed cells were diluted in 10 mL diluent (Isoton II; Beckman Coulter) and sonicated for 5 pulses of approximately 0.5 second each at low power ...
-
bioRxiv - Molecular Biology 2021Quote: ... Nuclease treated media was next transferred to 38-mL Ultra-Clear centrifuge tubes (Beckman Coulter) and centrifuged for 60 min at 133,000 x g at 4°C in a Sure Spin 630 36 mL rotor (Thermo Scientific) ...
-
bioRxiv - Molecular Biology 2021Quote: ... and layered onto the bottom of a 38-mL Ultra-Clear centrifuge tube (Beckman Coulter). Lower-density solutions were prepared by diluting homogenization buffer (250 mM sucrose ...
-
bioRxiv - Bioengineering 2022Quote: ... samples (10 mL) were first centrifuged at 7000Xg at 4°C for 30 minutes (Beckman Coulter Avanti J-E Series ...
-
Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: VLDL (d < 1.006 g/ml) was isolated via ultracentrifugation with a TLA120.2 rotor (Beckman Coulter). VLDL fractions were separated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE ...
-
bioRxiv - Bioengineering 2019Quote: ... Bacteria was concentrated to 1 mL by centrifugation (J2-MC, Beckman Instruments, Fullerton, United States) and stored in cryovials at −80°C (refrigerator type ...
-
bioRxiv - Molecular Biology 2022Quote: ... sonicated and then diluted in 10-20 ml of Isoton II diluent (Beckman Coulter #8546719) before measurement.
-
bioRxiv - Neuroscience 2022Quote: ... and 50% sucrose solutions (1.5 ml each) were layered into a centrifuge tube (Beckman, 331372). Brain total lysate (6 mg of protein ...
-
bioRxiv - Immunology 2022Quote: ... followed by centrifugation for 16 h at 40,000 rpm (MLS-50 swinging-bucket rotor, Beckman). Fractions with target protein of 1 ml were manually collected and concentrated to 200 µl by using Amicon Ultra-0.5 centrifugal filter unit with 100 kDa cutoff (EMD Millipore ...
-
bioRxiv - Biochemistry 2023Quote: ... 1.4 mL lysate was transferred to a tube and centrifuged (Beckman Coulter Microfuge 22R Centrifuge) at 18,000g for 10 min at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... 50%) in a 17 mL ultracentrifuge tube (Ultra-Clear, 16×102 mm, 344061, Beckman Coulter), and leaving overnight to allow for merging of the layers into a continuous gradient ...
-
bioRxiv - Cell Biology 2023Quote: ... The step gradient was formed by bottom loading 13 mL UltraClear tubes (Beckman Coulter, #344059) with a long metal cannula with 800 μL 2.5% ...
-
bioRxiv - Microbiology 2023Quote: ... pH 7.4) and loaded into a centrifuge tube (Beckman Cat. No. 344057, 5 ml capacity). One milliliter of 20% sucrose in 20 mM HEPES pH 7.4 ...
-
bioRxiv - Microbiology 2024Quote: ... 50% (w/v) sucrose) was filled into ultracentrifuge tubes (Polypropylene 10 ml Tube, Beckman Coulter), the sheared grappling hooks were gently layered on top ...
-
bioRxiv - Biophysics 2021Quote: ... 1ml acceptor LUV was diluted with 10 mM tris 100 mM NaCl and centrifuged to remove CsCl or sucrose (188,965 g, 37,500 rpm, 45 min, Beckman L8-80M, SW-60 rotor). The acceptor LUV pellet was resuspended in 1ml 20 mM tris 100 mM NaCl pH 7.4 buffer ...
-
bioRxiv - Biophysics 2022Quote: ... shortly sonified and centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1).) ...
-
bioRxiv - Biophysics 2022Quote: ... The samples were centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1). The supernatant ...
-
bioRxiv - Molecular Biology 2020Quote: ... and centrifuged at 10,000xg for 30 minutes at 4°C in a Beckman Coulter Optima XE-100 (fitted with a SW41Ti Rotor (Beckman Coulter, CA USA)) ...
-
bioRxiv - Cell Biology 2021Quote: ... Supernatant was discarded and cells were resuspended in 100 μL FACs buffer and sorted with a CyAn™ ADP Analyser (Beckman Coulter, Inc.) at the UCL GOSICH Flow Cytometry Core Facility ...
-
bioRxiv - Immunology 2022Quote: ... followed by ultracentrifugation at 110,000 × g for 90 min at 4°C on an Optima XPN -100 ultracentrifuge with SW32Ti rotor (Beckman Coulter, Brea, CA, USA). The isolated EVs was washed with PBS and followed with a secondary ultracentrifugation (110,000 × g for 90 min at 4°C) ...
-
bioRxiv - Genomics 2019Quote: ... 300 ng of high molecular weight genomic DNA was fragmented to 100 – 700bp (300bp average size) with a Covaris S2 instrument (LGC Genomics) and cleaned up with Agencourt AMPure XP (Beckman Coulter, Brea, USA). DNA library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs ...
-
bioRxiv - Cell Biology 2019Quote: ... The library fragments with a size range of 100-200 bp were selected with Agencourt AMPure XP beads (Beckman Coulter, Brea, CA, USA). Templates were prepared on the Ion Chef system using an Ion PI Hi-Q Chef kit (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: Lipoprotein fractions were separated via sequential ultracentrifugation after progressively increasing the solvent density (29) using the ultracentrifuge model Optima XPN-100 (Beckman Coulter, California, USA) and a Kontron 45.6 fixed-angle rotor at 37000 rpm and 4 °C for different time intervals ...
-
bioRxiv - Immunology 2021Quote: ... following by staining for 15 minutes at room temperature with a 1:100 dilution of a fluorescent anti-HIV-1 GAG antibody (KC57-FITC, Beckman Coulter reference 6604665) in permeabilization buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... supernatants were centrifuged at 100 000 x g for 70 min in ultracentrifugation using type 70 Ti rotor (Oprima XL-80K, Beckman, Brea, CA, USA) to pellet the EVs ...
-
bioRxiv - Microbiology 2023Quote: ... 2G12 was washed out with PBS twice followed by permeabilization with 0.2% Triton X-100 and staining for intracellular Gag with KC57-FITC (Beckman Coulter, Pasadena, CA; 6604665) diluted 1:100 in Dako antibody diluent ...
-
bioRxiv - Cell Biology 2023Quote: ... the resulting G-actin mix was ultracentrifuged at 90K rpm for 30 minutes at 4°C in an ultracentrifuge (Beckman, Optima TL 100) to remove actin oligomers and/or aggregates.
-
bioRxiv - Synthetic Biology 2023Quote: ... 600 ng of pool amplicons in 100 µl of volume were cleaned up with 1.0X volume of AMPureXP (Beckman-Coulter, West Sacramento, CA) beads according to the manufacturer’s directions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Differential ultracentrifugation was performed by a SW 32 Ti swinging bucket rotor in an Optima XPN-100 ultracentrifuge (Beckman Coulter, Brea, CA, USA). The cell-conditioned serum-free media ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells with the highest GFP expression (top 20%) were isolated on a Moflo XDP 100 cell sorter (Beckman Coulter Inc., Brea, CA, USA) and expanded ...
-
bioRxiv - Bioengineering 2023Quote: ... the supernatant was then taken and loaded into a 10% to 45% sucrose gradient solution and centrifuged at 360000 rpm for 3 h (Beckman Optima XE-100). 18 fractions from each sample were collected using a piston gradient fractionator (Biocomp ...
-
bioRxiv - Neuroscience 2019Quote: ... and 3% Percoll (1 ml each) in Polycarbonate centrifuge tubes (13 x 51 mm; Beckman Coulter). The gradients were then centrifuged for 15 min at 18,700 rcf ...
-
Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... The resuspended nuclei were then transferred to 38.5-ml ultracentrifuge tube on ice (Beckman Coulter, 344058). To the nuclei suspension ...
-
bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA) and cesium chloride (7.163 M) in 3.3 ml polyallomer centrifuge tubes (Beckman Coulter) to a final density of 1.700 g ml-1 ...
-
bioRxiv - Microbiology 2020Quote: ... 2.5 mM KCl) and then were successively underlaid into a 39 ml QuickSeal tube (Beckman Coulter). The rAAV-containing supernatant was then gently overlaid onto the gradient and then centrifugated for 2 hr in a 70 Ti rotor at 63,000 rpm,18°C ...
-
bioRxiv - Microbiology 2021Quote: ... and centrifuged for 2 h at 64,000 g in A27-8×50 mL rotors (Beckman Coulter) to sediment virus particles ...
-
bioRxiv - Microbiology 2022Quote: ... Samples were loaded into ultracentrifuge tubes (5.1 mL, Quick-Seal Round-Top Polypropylene Tube, Beckman Coulter) and spun at 20°C for 108 hours at 176,284 RCFavg (equivalent to 176,284 x g ...
-
bioRxiv - Microbiology 2022Quote: ... 5 mM CaCl2 buffer background) were placed in a 4 mL UltraClear ultracentrifuge tube (Beckman-Coulter), with the particle sample as the top layer ...
-
bioRxiv - Neuroscience 2023Quote: ... Eight fractions encompassing each OP concentration were collected into 30 mL thickwall polycarbonate tubes (Beckman 355631) as described in Figure S1 (F1 = 5% OP ...
-
bioRxiv - Cell Biology 2022Quote: ... Equal volume (2 ml i.e 1600 µg) of cell extracts were loaded in ultracentrifuge tubes (Beckman). Samples were ultracentrifuged at 100,000 g for 30 minutes at 4°C in a Optima XPN-100 ultracentrifuge (Beckman) ...
-
bioRxiv - Microbiology 2023Quote: ... 50 ml of cell culture was harvested by centrifugation for 10 min at 3000 xg (Beckman Coulter Allegra X30-R Centrifuge ...
-
bioRxiv - Physiology 2024Quote: ... The entire contents of a well were added to 10 mL of Isoton Buffer (Beckman Coulter) and particle diameter measured using a Multisizer 3 Coulter Counter (Beckman Coulter) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and supernatants were ultracentrifuged at 37 000 rpm (~100 000 g) and 10°C for 1h (Beckman Optima LE-80 K; rotor 70.1.Ti). Pellets were resuspended in 100μL of buffer (10 mM Tris-HCL ...
-
bioRxiv - Neuroscience 2022Quote: ... The samples were then spun at 31000 x g (18468 rpm) for 5 min at 4°C in a fixed-angle rotor (50.2 Ti rotor in Beckman Coulter Optima L-100 XP) with slow brake ...
-
bioRxiv - Neuroscience 2023Quote: ... PI (1 μl/100 μl) was added 5 minutes before starting the reading on the flow cytometer CytoFlex S (Beckman Coulter, Brea, CA, USA). Results were shown and analyzed through CytExpert flow cytometry analysis software.
-
bioRxiv - Cancer Biology 2021Quote: ... The filtered sample was then either used as “functional conditioned media” for survival assays or transferred to a Beckman Optima TL-100 Ultracentrifuge (Beckman Coulter Life Sciences, Brea, CA) for sEV isolation ...
-
bioRxiv - Cell Biology 2020Quote: ... and the suspensions were ultracentrifuged at 100,000 × g for 1 h in MLS-50 rotor (Beckman Coulter) to wash the samples (final sample ...
-
bioRxiv - Microbiology 2021Quote: ... the number of metabolically active cells per mL were counted using a cytoFLEX S (Beckman Coulter, USA). Data were analyzed using CytExpert (Beckman Coulter ...
-
Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... The resuspended EVs were then transferred to a 1.5-ml ultracentrifuge tube (Beckman Coulter, Cat no 357448) and centrifuged at 160,000 g at 4 °C for 1.5 h in a fixed-angle rotor to obtain the sediment termed ‘miniUC pellet’ ...