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Citations for Beckman :
351 - 400 of 446 citations for Interleukin 10 Receptor Alpha IL10RA Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The absorbance was recorded at 1 min intervals for 10 min at 340 nm at 30°C using a spectrophotometer (DU 800, Beckman Coulter). The enzymatic activity was determined as the change in absorbance/min/mg protein.
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bioRxiv - Molecular Biology 2021Quote: ... as determined by flow-cytometry with anti-CD34 antibody (#581-PE; Beckman Coulter, measured on Beckman Coulter FC500). CD34+ cells were seeded at a density of 50.000 cells/well in a 12 well plate in CellgroSGM (CellGenix ...
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bioRxiv - Microbiology 2022Quote: ... a fluorescent labeling was first made with anti-p24 antibody (Clone: KC57, Beckman Coulter, 6604667; 1/200 dilution). Then ...
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bioRxiv - Immunology 2023Quote: ... and intracellularly stained with the Fluorescein isothiocyanate-labeled KC57 (anti-HIV-1 p24) antibody (1:200) (Beckman Coulter) or a mixture of both antibodies ...
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bioRxiv - Immunology 2021Quote: ... the cells were washed and resuspended in RPMI with 10% of SFB and EDTA 0,05mM and submitted to cell sorting in Moflo Astrios® cytometer (Beckman Coulter®). The gate strategy and neutrophil yield are supplied in the supplementary figure 1.
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bioRxiv - Genetics 2021Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase with addition of the buffer incubated for 60 min at 37 °C followed by inactivation with 1 μL of 10% SDS and purification using RNA clean SPRI beads (Beckman Coulter, A63987).
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bioRxiv - Microbiology 2019Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987). Data were collected on Accuri C6 (BD Biosciences – U Mass ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting cDNA was PCR-amplified to about 10–20 ng/μl using a high-fidelity DNA polymerase and purified with the Agencourt AMPure XP Kit (Beckman Coulter Genomics). The cDNA samples were pooled based on the ratios according to the RNA concentrations of the input samples ...
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bioRxiv - Molecular Biology 2019Quote: ... was loaded onto 4 ml of a 10% −30% (w/v) linear sucrose gradient and centrifuged at 74,000g at 4°C for 6 h (Beckman MLS-50 rotor). Fractions (250 µl ...
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bioRxiv - Genomics 2019Quote: ... Cells were suspended with 300 μl 1% FBS /HBSS (with 10 mM sodium butyrate) and subjected to FACS (Beckman Coulter MoFlo XDP) sorting for tdTomato positive cells ...
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bioRxiv - Molecular Biology 2019Quote: ... 4 ml of 10 % and 3.5 ml of 5 % iodixanol in a 17 ml Thinwall Polypropylene Tube (Beckman Coulter, Fullerton, California, USA). The DG was centrifuged 18 h at 100,000 g and 4 °C using SW 32.1 Ti rotor (Beckman Coulter ...
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bioRxiv - Biochemistry 2020Quote: ... was layered on NaBr δ=1.28 g/mL and centrifuged at 207,000g for 22 h at 10 °C on a Beckman L8M centrifuge (Beckman, Palo Alto, CA). A tube layered with 20 mM Tris-HCl in lieu of the sample was used as a blank for density calculations ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor) to separate the soluble and detergent-enriched phases ...
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bioRxiv - Microbiology 2019Quote: ... Cytoplasmic lysate was layered onto 10-50% sucrose gradients (in polysome buffer with 100 μg/mL cycloheximide) in thinwall polypropylene ultracentrifuge tubes (Beckman Coulter; 331372). Gradients were then spun at 38,000 RPM for 2 h in a SW41Ti rotor (Beckman Coulter ...
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bioRxiv - Molecular Biology 2020Quote: ... diluted in 9 mL of 10 mM Tris-HCl buffer and centrifuged for 45 min at 45.000 rpm (Beckman 70.1 Ti Rotor). Pellets were suspended in the same buffer and analyzed by SDS-PAGE using 12.5% polyacrylamide gel ...
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bioRxiv - Microbiology 2020Quote: ... virions were purified from the infected S2 cells by centrifugation at 8,000 RPM for 10 minutes at 4 °C using a Beckman GSA rotor (Beckman Coulter, Brea, California). The supernatant was then collected and centrifuged at 8,000 RPM for 30 minutes at 4 °C using a Beckman GSA rotor ...
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bioRxiv - Microbiology 2021Quote: ... RNA in the aqueous phase was purified and collected in 10 μL of RNase-free water using Agencourt RNAClean XP (Beckman Coulter, A63987) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... The insect cells were harvested after 4 days by centrifugation at 300 x g for 10 min at 4 °C in an Avanti J-26S ultracentrifuge (JLA-9.1000 rotor, Beckman Coulter, Brea, CA). The cell pellet was resuspended in 5 ml ice-cold PBS and stored at −80 °C for further use ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: DNA was extracted from the gut samples using 10% Chelex (24) and purified with AMPureR XP beads (Beckman Coulter, Brea, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Samples were eluted in 20 μL of elution buffer and 10 μL of each sample was pooled and concentrated to 20 μL using 0.7x AMPureXP beads (Beckman Coulter, Brea, CA). The final pooled sample was quantified using a Qubit Fluorometer and High Sensitivity DNA assay (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... supernatant containing pseudovirions was harvested by centrifugation at 2,000 rpm for 10 min and concentrated by Optima XPN-100 Ultracentrifuge (Beckman Coulter, California, USA). For pseudovirus infection ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor) to separate the soluble and detergent-enriched phases ...
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bioRxiv - Microbiology 2022Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987) or 1:300 KC57-RD1 (numbers) ...
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bioRxiv - Neuroscience 2023Quote: ... The nuclei suspension was layered onto 10 mL of 29% OptiPrep solution (Buffer + 50% OptiPrep) in a 38.5 mL Ultra-Clear tube (Beckman-Coulter, CA, USA) before being centrifuged at 7,333g for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated at RT for 30 minutes and centrifugated at 20,000 x g for 10 minutes (Avanti J-30I Centrifuge, Beckman Coulter, Brea, CA). The supernatant was collected and diluted 1:5 with incubation buffer (final concentration ∼204 cells mL-1) ...
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bioRxiv - Cell Biology 2023Quote: ... 150 μL of the recovered sample was mixed with 150 μL of 60% Optiprep and layered with 200 μL of 10% Optiprep and 150 μL of the reconstitution buffer in a 0.8 mL tube (Beckman coulter Cat. #344090). The tube was centrifuged at 40,000rpm for 90 minutes in a SW-55 rotor ...
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HIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus MaturationbioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in STE buffer and banded out over an OptiPrepTM step gradient (10, 20, 30% OptiPrepTM (v/v)) with a SW55 Ti rotor (Beckman Coulter, CA) at 45,000 rpm for 3 h ...
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bioRxiv - Microbiology 2024Quote: ... Saliva was collected by expectoration into tubes on ice followed by centrifugation for 10 minutes at 4°C at 4000 x g (Beckman SX4750 rotor) to remove large debris and bacterial aggregates ...
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bioRxiv - Microbiology 2024Quote: ... The resulting cDNAs were PCR-amplified to about 10-20ng/µl using a high-fidelity DNA polymerase and purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics). Pooled libraries were sequenced on an Illumina NextSeq 500 platform at the Core Unit SysMed at the University of Würzburg for bacterial in vitro samples and on an Illumina Novaseq at the genome analytics core facility of the Helmholtz Centre for Infection Research (HZI ...
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bioRxiv - Microbiology 2019Quote: ... Intracellular Gag staining was carried out using a Gag monoclonal antibody conjugated to Phycoerythrin (KC-57 RD1 Beckman Coulter). 1×105 cells from a HIV GPV-zip coded library were washed once with FACS buffer and fixed with 100 µl of BD cytofix for 10 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2022Quote: ... The cells were stained with a panel of monoclonal antibodies containing: ECD CD16 clone 3G8 (Beckman Coulter; cat.no. A33098), BV711 CD45 clone HI30 (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with the fluorescently labeled anti-p24 antibody (Clone: KC57, Beckman Coulter, 6604667; 1/200 dilution).
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... and then stained for flow cytometry using the CD34-phycoerythrin (PE) antibody (PE-pool, Beckman Coulter, IM1459U, Brea, CA). RNA-sequencing libraries were then prepared from RNA isolated from the bulk mutant AML bone marrow cells and the normal CD34+ bone marrow cells using TruSeq Stranded Total RNA kit (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... unbound PKH67 dye and unbound antibodies by overnight bottom-up sucrose density gradient (SDG) ultracentrifugation at 192,000 g (SW40 rotor Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: The TCR V beta repertoire kit contained antibodies to over 76% of V βeta families (Beckman Coulter, Fullerton, CA). The cells were stained with these antibodies in combination with tetramers or dextramers for 20 minutes at RT to determine the TRBV repertoire of antigen specific cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Platelet concentration was obtained by flow cytometry using an antibody against human CD41 labelled with PC7 (6607115, Beckman Coulter) and MP-count beads (6607115 ...
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bioRxiv - Microbiology 2019Quote: 100 μℓ of cell suspension was added to 10 mℓ of PBS and cell size distribution and cell number was measure in a Z2 Coulter Counter (Beckman Coulter, Nyon, Switzerland), with a 100μm orifice ...
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bioRxiv - Immunology 2021Quote: Immune cell types in Cohort 1 were measured from whole blood by 10-color flow cytometry with Navios flow cytometer (Beckman Coulter, CA, USA). Staining and gating strategies were previously described in detail by Aguirre-Gamboa et al ...
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bioRxiv - Bioengineering 2021Quote: ... 10 µl of which was added to 10 ml of ISOTON® diluent and counted on the COULTER COUNTER® Z1 Series Particle Counter (Beckman Coulter). From this count ...
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bioRxiv - Systems Biology 2020Quote: ... insoluble material was pelleted down at 2500 RCF for 10 minutes at 4C and the collected supernatant was subjected to ultracentrifugation (35000 RPM for 30 min. at 4C, Beckman-Coulter Optima Ultracentrifuge) to remove bacteria ...
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The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: ... samples were layered onto a 10–50% sucrose gradient in lysis buffer and centrifuged in an SW40Ti rotor (Beckman Coulter, High Wycombe, UK) at 38,000 rpm for 2 h ...
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bioRxiv - Synthetic Biology 2022Quote: ... 20 μL reactions were prepared by dispensing 1 μL of each 10 μM reverse primer into the wells of a 96-well PCR plate using the Echo liquid handler (Beckman Coulter, Brea, CA). A mastermix consisting of polymerase premix ...
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bioRxiv - Biochemistry 2021Quote: ... was arrayed in a minimum of a triplicate across a 10 kDa MWCO 96-well microdialysis plate (SWISSCI Diaplate™) and sealed with aluminum foil seals (Beckman Coulter 538619) to create the protein chambers ...
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bioRxiv - Cell Biology 2020Quote: ... followed by serial centrifugation at 100’000 g for 120 min at 10 °C (Beckman fixed-angle TL110 rotor; Beckman Optima Max-TL ultracentrifuge). The so obtained P100 pellets ...
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bioRxiv - Microbiology 2021Quote: ... the SBV samples were purified via sucrose density gradient centrifugation (10–50% range) at 32,500 rpm for 4 h with an SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter Inc., Brea, CA). The SBV band that corresponded to the 40% sucrose gradient was harvested and then centrifuged at 25,000 rpm for 12 h ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting lysate was loaded on top of 10 ml 29% iso-osmolar OptiPrep solution in a conical 30 ml centrifuge tube (Beckman Coulter, SW28 rotor). Samples were centrifuged at 10,100 × g (7,500 r.p.m. ...
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bioRxiv - Microbiology 2023Quote: MCP2201-LBD was diluted to 75 μM in SEC buffer with or without 10 mM ligand.Sedimentation velocity analytical ultracentrifugation experiments were performed on a Beckman XL-I analytical ultracentrifuge (Beckman Coulter, Brea, CA, USA) at 4 °C with a rotor speed of 60,000 rpm for 7 h ...
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bioRxiv - Cancer Biology 2023Quote: ... the supernatant was layered on top of a linear sucrose gradient (10–50% sucrose) loaded in a SW40 Ti swinging-bucket rotor (Beckman Coulter, Krefeld, Germany) and centrifuged (33.500 rpm/4°C/3h ...
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bioRxiv - Molecular Biology 2023Quote: ... aggregates and debris were removed by centrifuging at 1,000 ×g for 10 minutes at RT (Avanti J–15R; Beckman Coulter Inc., Brea, USA). Next ...
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bioRxiv - Cell Biology 2023Quote: ... either the dye was solubilized with 10% (v/v) acetic acid and the absorbance was measured at 570 nm in a spectrophotometer microplate reader (Beckman Coulter, Model AD340), or alternatively ...