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Citations for Beckman :
1 - 50 of 202 citations for IL 5 Rat CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... CHO MPs were isolated at 28,000g for 30 minutes by ultracentrifugation (Beckman Coulter Optima LE-80K Ultracentrifugation ...
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bioRxiv - Bioengineering 2021Quote: CHO exosomes were isolated from the supernatant after isolating CHO-MPs by filtering (0.22 μm) and centrifuged at 100,000g for 90 minutes (SW-28 rotor, Beckman Coulter Optima LE-80K). Then ...
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bioRxiv - Immunology 2020Quote: ... during 30min and then transferred into a DuraClone IF T Helper cell tube (CD3, CD4, IL-4, IL-17A, IFNγ) (Beckman Coulter), using the IntraPrep fixation permeabilization kit (Beckman Coulter) ...
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bioRxiv - Neuroscience 2020Quote: ... Serum 17β-estradiol levels in female rats and serum testosterone levels in male rats were assayed using commercially available radioimmunoassay (RIA) kits from Beckman Coulter (Brea ...
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bioRxiv - Microbiology 2024Quote: ... horseradish peroxidase-conjugated anti-rat IgG (Beckman Coulter), or horseradish peroxidase-conjugated anti-mouse IgG (CST ...
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bioRxiv - Cell Biology 2023Quote: ... secondary anti-rat Cy3 (Beckman Coulter, Ref. 712-166-153), HRP-NHS (AAT Bioquest ...
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bioRxiv - Cell Biology 2023Quote: ... secondary anti-rat-HRP (Beckman Coulter, Ref. 712-035-153), secondary anti-rat Cy3 (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... all from BD Biosciences as well as IL-2 PE (clone N7.48A, Beckman, Cat IM2718U) and TNF PCP-Cy5.5 (clone MAb11 ...
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bioRxiv - Immunology 2021Quote: ... The levels of IL-10 were evaluated in NAVIOUS EX flow cytometer (Beckman Coulter), and the results were analyzed using Kaluza ...
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bioRxiv - Genomics 2023Quote: Rat tissues were disrupted in GenFind v2 lysis buffer (Beckman Coulter, Indianapolis, IN) with a tissue ruptor (Omni International ...
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bioRxiv - Genetics 2020Quote: ... HS rat DNA was extracted from spleen tissue using the Agencourt DNAdvance Kit (Beckman Coulter Life Sciences ...
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bioRxiv - Genetics 2022Quote: ... 5 mL (Beckman Coulter, B23317) using a DynaMag™-2 Magnet magnetic stand (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... labelled BM-MDSCs were stained with the Annexin V-FITC Apoptosis Detection Kit (Beckman Coulter, IL, Italy) following manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Rat tissue powders were further disrupted using Agencourt RNAdvance tissue lysis buffer (Beckman Coulter, Brea, CA) with a tissue ruptor (Omni International ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-4 and IFN-γ concentrations were determined using DTX 880 Multimode Detector (Beckman Coulter, Inc., Brea, CA, USA) set to 450 nm.
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bioRxiv - Immunology 2022Quote: ... Every 4 days cells were fed by replacing 25 μl of media with fresh I10 containing irradiated 2.5 × 103 3T3-msCD40L cells and 50 ng/mL IL-21 using a Biomek NXP liquid handler (Beckman Coulter). Cells were cultured for 2-3 weeks before harvesting the final supernatant and either freezing the cultures in the 384-well plate (50ul freezing media per well (FBS + 10% DMSO)) ...
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bioRxiv - Microbiology 2021Quote: ... and 1.3 mL of 5% (v/v) glycerol in TNE buffer) in 5 mL thin-walled Ultraclear centrifuge tubes (Beckman Coulter). Viral nucleocapsids were pelleted by centrifugation at 23,000 rpm in an SW 55 Ti rotor for 1 h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... gradient (6 ml 17 %, 6 ml 25 %, 5 ml 40 % and 5 ml 60 %) contained within an OptiSeal tube (Beckman,). Gradients were spun at 242 000 g at 16 °C and the viral fraction was harvested from the 40 % fraction with a 21-gauge needle ...
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bioRxiv - Pathology 2024Quote: RNA from rat liver tissue was snap frozen in RNAlater and lysed using lysis buffer (RNAdvanced Kit, A32646, Beckman Coulter), 20µL Proteinase K on a TissueLyser II (Qiagen ...
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bioRxiv - Systems Biology 2020Quote: ... and 1 mL of 5% (w/v) iodixanol solutions on top of each other in a 5 mL open top polyallomer tube (Beckman Coulter). Crude exosomes were resuspended in 200 µL PBS and overlaid on top of the gradient ...
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bioRxiv - Physiology 2019Quote: ... Agencourt AMPure XP PCR purification kit (5 ml Beckman Coulter Part No ...
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bioRxiv - Cell Biology 2022Quote: ... we used a CytoFLEX 5 flow cytometer (Beckman Coulter) and the CytExpert version 2.3 software ...
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bioRxiv - Neuroscience 2022Quote: ... anti-CD90 (IM1839U / Beckman Coulter / F15-42-1-5), anti-CD146 (A07483 / Beckman Coulter / TEA1-34) ...
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bioRxiv - Immunology 2023Quote: ... KIR2DL1/S1 (Beckman Coulter, clone EB6B: PE-Cy5-5) was performed at room temperature for 30 minutes in PBS (w/o Ca or Mg ...
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bioRxiv - Neuroscience 2023Quote: ... 5 ml of complete Neurobasal medium were added and centrifuged for 5 min at 335 x g (Beckman Coulter Allegra X-12R centrifuge). The complete Neurobasal medium consisted of ...
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bioRxiv - Cell Biology 2021Quote: ... for 5 minutes at 4°C with TLA100 rotor (Beckman). 100 µg cell lysate was brought to a final volume of 250 µl BRB80 supplemented with 1 mM GTP ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 mM MgCl2) and spun in an SW41Ti rotor (Beckman) for 20 hours at 30,900 rpm ...
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bioRxiv - Immunology 2021Quote: ... for 5 minutes (TEMP) and labeled with Viakrome 808 (Beckman Coulter) and fluorochrome-labeled antibodies against CD4 ...
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bioRxiv - Immunology 2022Quote: ... anti-NKG2A-APC (clone Z199; 5 μL; cat. # A60797) from Beckman Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×10^6 cells counted by Multisizer 4e Coulter Counter (Beckman) were plated on a 60 mm plate and serum starved before analysis unless otherwise indicated ...
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bioRxiv - Neuroscience 2024Quote: ... the supernatant was transferred into a 5 mL polypropylene tube (Beckman Coulter), and PBS was added to reach a 4.5 mL tube filling and centrifuged at 120,000xg for 100 min at 4°C in the L-60 Optima Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Molecular Biology 2019Quote: ... 500 mM sucrose) in a 5 × 44 mm micro centrifuge tube (Beckman Coulter) and sedimented by centrifugation (12000 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... Crosslinking reactions were loaded on 600µl 5∼35% glycerol gradients (Beckman 344090 tubes) in BC300N buffer and centrifuged for 4hr at 55,000rpm at 4°C in a TLS-55 rotor (with Beckman 356860 adaptors) ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were centrifugated at 1000 g for 5 min at 4°C (Beckman Coulter Microfuge 22R ...
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bioRxiv - Microbiology 2020Quote: ... Cells were pelleted at 200 x g 5 min in Allegra centrifuge (Beckman) and then resuspended in complete RPMI ...
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bioRxiv - Bioengineering 2019Quote: ... 5′ cDNAs were purified using the Agencourt AMPure XP Kit (Beckman Coulter Genomics) and analysed by capillary electrophoresis before sequencing the single-end libraries using the Illumina NextSeq 500 system and a MID 150 Kit with 75 bp read length.
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bioRxiv - Cancer Biology 2020Quote: ... 3 and 5 and counted using a particle counter (Z1; Beckman Coulter, Inc.). Experiments were carried out in triplicate ...
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bioRxiv - Cell Biology 2023Quote: ... and then spun for 5 minutes in a chilled airfuge (Beckman Coulter, 340401) at around 80,000 rpm ...
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bioRxiv - Microbiology 2024Quote: ... gradient (25 – 39 % OptiPrep, 309600 x g, 5 h, SW60Ti rotor (Beckman Coulter). Purification of VLPs was realized accordingly ...
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bioRxiv - Molecular Biology 2024Quote: ... Discontinuous sucrose gradient was prepared in 5 ml ultracentrifuge tubes (Beckman Coulter, C14279) with 2 ml of 1.2 M sucrose solution (1.2 M sucrose ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 mM MgCl2) and centrifuged at 36,000 rpm [SW40 Ti rotor (Beckman Coulter, inc)] for 2.15h at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... and platelets were counted by a Hematology Analyzer (Beckman Coulter Act Diff 5 CP) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 5 ml of this mix was transferred to a 17-ml ultracentrifuge tube (Beckman) and overlayed with the following sucrose solutions ...
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bioRxiv - Molecular Biology 2021Quote: ... Sucrose density gradients (5%–45% w/v) were freshly made in SW41 ultracentrifuge tubes (Beckman) using a Gradient Master (BioComp Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 mM MgCl2) and centrifuged at 45,000 rpm (SW 55 Ti rotor, Beckman Coulter, Inc.) (adapted from Liang et al ...
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bioRxiv - Immunology 2019Quote: ... 5 µl of cDNA per cell was cleaned using Agencourt AMPure XP beads (Beckman Coulter) at a 1.0x ratio on a Hamilton STAR liquid handler (Hamilton Robotics) ...
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bioRxiv - Microbiology 2021Quote: ... Product were PCR amplified by 5 cycles and cleaned up using SPRI beads (Beckman Coulter) and subjected to paired-end 300bp sequencing on Illumina Miseq ...
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bioRxiv - Biochemistry 2023Quote: ... Ultracentrifugation was carried out for 5 hours at 133,000xg on a SW60-Ti (Beckman Coulter) rotor to resolve each gradient ...
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bioRxiv - Cancer Biology 2023Quote: ... After 5-7 days cells were counted using a Z1 Coulter Particle Counter (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: ... pH 7.4) and loaded into a centrifuge tube (Beckman Cat. No. 344057, 5 ml capacity). One milliliter of 20% sucrose in 20 mM HEPES pH 7.4 ...