Labshake search
Citations for Beckman :
601 - 650 of 1114 citations for IL 4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The soluble fraction was collected with centrifugation at 20,000 rpm (46,502 rcf) at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter) and applied to the HisTrap HP column (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... lysates were centrifuged at 60,000 x g (4 °C) for 45 min (Beckman Avanti JXN-26 (rotor: JA 25.5)) and immediately supplemented with 10 mL pre-washed Ni2+-NTA agarose (QIAGEN ...
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bioRxiv - Biophysics 2024Quote: ... Each seed mix was clarified using an ultra-centrifuge (Beckman Optima MAX-XP, 90,000 RPM, 4°C, 5 min) then snap-frozen in small aliquots and stored at -80°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 μg/mL cycloheximide) for 2 h at 40,000 g at 4 °C using an SW41 rotor (Beckman Coulter). Gradients were fractionated using Teledyne Isco Tris Peristaltic Pump and fractions were collected and pooled according to the UV trace ...
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bioRxiv - Cell Biology 2024Quote: ... samples were ultracentrifuged at 160,000 xg for 2 h at 4 °C in ultracentrifuge tubes (Beckman Coulter Cat #328874). The resulting pellet contained the insoluble AMB-1 cell fraction and the supernatant contained the soluble fraction ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was then subjected to ultracentrifugation at 100,000 x g for 2 h at 4 °C to sediment small EVs (Optima L-80K ultracentrifuge, Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 M sucrose) at 100,000 rpm (543,000 × g) and 4°C for 1 h with a TLA110 rotor (Beckman Coulter) and an Optima MAX-TL Ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2023Quote: ... supernatants from the SF samples and cellfree human and equine neutrophil cell culture supernatants were transferred to SW40 tubes (Beckman Coulter Inc., Brea, United States). They were subjected to centrifugation at 10,000 ×g for 35 min at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Excess dye was removed by ultracentrifugation of the virus through a 10% sucrose cushion for 2 h at 107,000 × g and 4°C using an SW41 rotor (Beckman Coulter). Labeled virus pellets were resuspended at a viral protein concentration of 1 mg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... 10 U/mL SuperASE-In and was ultracentrifuged at 100,000 × g for for 16 h at 4 °C in a SW Ti41 rotor (Beckman Coulter). After removing the supernatant ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by ultracentrifugation at 30,000 × g 30 min at 4°C using a Ti45 rotor (Beckman Coulter) twice ...
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bioRxiv - Biophysics 2020Quote: ... Escherichia coli BL21 cells overexpressing α-synuclein were collected by centrifugation (20 min, 4000 rpm, 4 °C) in a JLA-8.1000 rotor in a Beckman Avanti J25 centrifuge (Beckman Coulter), resuspended in lysis buffer (10 mM Tris ...
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bioRxiv - Biophysics 2020Quote: ... The insect cells were harvested after 4 days by centrifugation at 300 g for 10 min at 4 °C in an Avanti J-26S ultracentrifuge (JLA-9.1000 rotor, Beckman Coulter). The cell pellet was resuspended in 5 ml ice-cold phosphate buffered saline (PBS ...
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bioRxiv - Biophysics 2020Quote: ... and centrifuged at 45000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter). The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...
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bioRxiv - Cell Biology 2020Quote: ... Crude mitochondria were loaded on top of the chilled gradient and separated at 100,000 Å∼ g for 1 h at 4°C (SW41 rotor; Beckman). Intact purified mitochondria were recovered from a brown band at around 16% Histodenz™ concentration ...
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bioRxiv - Cell Biology 2020Quote: ... and the gradient was centrifuged for 60 min at 24,700 × g at 4 °C using a TLS-55 swinging bucket rotor (Beckman Coulter). Purified mouse POS were resuspended in DMEM/Ham’s F-12 containing 2.5% sucrose ...
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bioRxiv - Cell Biology 2020Quote: ... lysed by sonication (UD-201 ultrasonic disrupter, Tomy Seiko), and centrifuged at 50,000 rpm (70 min, 4°C) with a 70 Ti rotor (Beckman Coulter). GST-His6-tagged LC3B-His12 and GATE-16-His12 proteins in the supernatants obtained were isolated by mixing with COSMOGEL GST-Accept beads (50% slurry ...
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bioRxiv - Immunology 2021Quote: ... the culture medium containing the lentiviral particles was collected and ultracentrifuged at 25,000 rpm for 90 min at 4 °C using a Beckman SW28 ultracentrifuge rotor (Beckman Coulter) for the obtention of concentrated virus ...
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bioRxiv - Microbiology 2019Quote: ... Samples were centrifuged at 27,000 x rpm for 16 hrs at 4°C in an Optima L-100 XP ultracentrifuge (Beckman Coulter) using a SW32Ti rotor.
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bioRxiv - Microbiology 2020Quote: ... sanguinis was grown as described above and centrifuged for 10 minutes at 3,740 x g in an Allegra X-142 centrifuge at 4°C (Beckman-Coulter). The supernatant was discarded and the cells were resuspended in BHI prior to inoculation ...
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bioRxiv - Neuroscience 2021Quote: ... PMCA products were centrifuged at 45,000 rpm for 2.5 h at 4 °C (Optima™ Max Ultracentrifuge, Beckman Coulter, USA), the supernatants discarded and the pellets resuspended in an equal volume of PBS.
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM DTT and centrifuged for 16 h at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). The fractions were subjected to SDS-PAGE and Coomassie blue staining.
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bioRxiv - Molecular Biology 2021Quote: ... The samples were centrifuged at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM DTT and centrifuged for 16 h at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). The fractions ...
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bioRxiv - Molecular Biology 2020Quote: ... Gradients were centrifuged at 39 000 rpm for 2.5 h at 4°C in an Optima L-100XP ultracentrifuge (Beckman– Coulter) using the SW41Ti rotor with brake ...
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bioRxiv - Cell Biology 2020Quote: ... The insect cells were harvested after 4 days by centrifugation at 300 g for 10 min at 4 °C in an Avanti J-26S ultracentrifuge (JLA-9.1000 rotor, Beckman Coulter). The cell pellet was resuspended in 5 ml ice-cold phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... and centrifuged at 45000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter). The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...
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bioRxiv - Molecular Biology 2019Quote: ... for 2 h at 4°C and then further purified using CsCl equilibrium gradient ultracentrifugation at 35,000 rpm (Beckman SW56) for 18 h at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... was transferred to a new polycarbonate ultracentrifuge tube and centrifuged at 100000 xg for 70 minutes at 4°C (Beckman Coulter Optima L-80 XP Ultracentrifuge ...
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bioRxiv - Cell Biology 2019Quote: ... all proteins were pre-cleared of aggregates via centrifugation at 80,000 RPM for 15 min at 4°C in a TLA100 rotor (Beckman Coulter).
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bioRxiv - Molecular Biology 2020Quote: ... The total bacterial lysate was centrifuged at 48,384 x g at 4 °C for 1 hr (Beckman JA-25.50 rotor), and the supernatant was applied to a 5 mL HisPur Ni-NTA resin (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... The unbound cyt c was separated from liposome-bound cyt c by ultracentrifugation at 435,000 g for 2 hours at 4 °C in an Optima TLX ultracentrifuge with a TLA-100 rotor (Beckman Coulter). Immediately after the ultracentrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... actins were cleared by highspeed centrifugation at 316,613 x g for 1 hour at 4°C in a TLA100 rotor (Beckman Coulter). Only the top 80% of the supernatant was collected to perform TIRF microscopy ...
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bioRxiv - Developmental Biology 2020Quote: ... Separation of testis cell populations was performed by centrifugal elutriation using a JE-5.0 elutriation system and a 4-ml standard elutriation chamber (Beckman Coulter). The assembly of the system followed the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2020Quote: ... the EV pellets were resuspended in 3 ml 0.32 M sucrose (diluted in PBS) solution and centrifuged again at 100,000xg for 60 minutes at 4°C (SW60 rotor, Beckman Coulter). Sucrose supernatant was removed and pellet was resuspended in 500 μl phosphate-buffered saline containing 0.01% bovine serum albumin (0.2 μm filtered ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was continued for 24 h before harvesting cells by centrifugation (4°C, 8000 rpm, 10 min in Beckman Coulter Avanti JXN-26 centrifuge ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was continued for 18 h before harvesting cells by centrifugation (4°C, 8000 rpm, 10 min in Beckman Coulter Avanti JXN-26 centrifuge ...
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bioRxiv - Biophysics 2021Quote: Pooled seminal plasma from stallions was centrifuged for 30 min at 10,000g and 4°C (Beckman Coulter, BC - rotor SW32Ti) to pellet cell debris and larger molecular complexes ...
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bioRxiv - Plant Biology 2020Quote: ... The sucrose gradient was centrifuged at 154,300 × g for 15 h at 4 °C using a SW 41 Ti rotor (Beckman Coulter). The LHCII proteoliposomes separated from empty liposomes were collected dropwise from the bottom of the tube and used for analysis.
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bioRxiv - Cancer Biology 2021Quote: ... Tubes were then spun at 10,000 x g for 20 minutes at 4 °C in an ultracentrifuge (Beckman, L8-70M). The supernatant was filtered using 0.2 μM syringe filters (Millipore Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... The pellet was washed with PBS and ultracentrifuged at 100,000 × g 1h at 4°C to obtain small vesicles (TL-100 Ultracentrifuge, Beckman Coulter). The final pellet was resuspended in PBS for subsequent experiments ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted sample was collected by centrifugation at 2000 rpm for 1 minutes 4 °C in a F241.5P rotor using a 149 Microfuge 22R centrifuge (Beckman Coulter). One aliquot of the eluted sample was resolved on SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... 20% FBS containing RPMI was ultracentrifuged for 18□hours at 100,000□g at 4□°C in a 45Ti fixed angle rotor using polycarbonate tubes (both from Beckman Coulter). After ultracentrifugation ...
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bioRxiv - Microbiology 2020Quote: ... the sample was centrifuged for 16 h at 4°C and 100,000 rcf using a type 70 Ti rotor (Beckman Coulter). The pellet was gently washed with 500 μl of storage buffer (lysis buffer C + 10% (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... The plant debris was removed by centrifuging at 16 000 g for 15 minutes at 4°C in a JA-25.50 rotor and Avanti J- 20 XP centrifuge (Beckman Coulter). The clear supernatant was then gently poured on top of 8 ml sucrose cushion (100 mM Tris·HCl (pH 8.0) ...
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bioRxiv - Microbiology 2021Quote: ... The density gradient centrifugation was performed at 141,000 g and 4 °C for 20 h in a SW28 swing rotor (Beckman Coulter). A total of 18 fractions were collected starting from the top.
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bioRxiv - Neuroscience 2020Quote: ... Samples were centrifuged at 15,600 rpm and 4°C using a Swi32T swinging bucket rotor and Beckman ultracentrifuge (Beckman Coulter) for 45 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Archaella were pelleted from the supernatant by ultracentrifugation (60,000 × g, 90 min, 4 °C; Beckman Optima LE-80K, rotor 70Ti), resuspended in 150 μl 0.1 M HEPES/NAOH pH 7.0 and purified for 48 h (250,000 × g ...
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bioRxiv - Molecular Biology 2020Quote: ... The sucrose gradients were centrifuged at 200,000 x g avg at 4°C for 173 min using a SW40Ti rotor (Beckman Coulter). After the spin ...
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bioRxiv - Microbiology 2020Quote: MT-2 cells were collected and washed three times by pelleting at 200 x g for 5 min at 4°C in an Allegra centrifuge (Beckman) and resuspending cells in ice-cold PBS ...