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Citations for Beckman :
251 - 300 of 2261 citations for Human Single stranded DNA binding protein 4 SSBP4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... GFP-positive cells were isolated into single clones using a cell sorter (MoFlo Astrios EQ; Beckman Coulter, Brea, CA, USA). For the confirmation of each gene KO ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... GFP-expressing cells were sorted into single-cell clones by flow cytometry with a Moflo Astrios EQ instrument (Beckman Coulter). Clones were expanded and assessed for gene knockout by immunoblot analysis.
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bioRxiv - Cancer Biology 2020Quote: ... DNA was extracted from fresh frozen tissue or cell lines using the Agencourt DNAdvance Beckman Coulter kit (#A48705, Beckman Coulter, Brea, CA, USA), fragmented and submitted to a series of enzymatic steps including End-repair and Adenylation ...
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bioRxiv - Cell Biology 2023Quote: ... were collected from microplates and washed twice in PBS and then resuspended in 50 μl of detergent (DNA-Prep Reagent Kit; Beckman Coulter, Brea, CA) containing 10 ng/ml propidium iodide (DNA-Prep Reagent Kit ...
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bioRxiv - Genomics 2024Quote: ... All DNA samples were cleaned three times using Agencourt AMPure beads prior to starting the 1D library kit (Beckman Coulter, Inc., CA, USA). Genomes were assembled with Canu v1.6 with a minimum read length of 5 kbp (45) ...
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bioRxiv - Immunology 2023Quote: ... GEMs were broken and complementary DNA (cDNA) was cleaned up with DynaBeads MyOne Silane Beads (10x Genomics, No. 2000048) and SPRIselect Reagent Kit (Beckman Coulter, No. B23318). Full-length ...
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bioRxiv - Biophysics 2019Quote: ... Total volume was centrifuged at 10000 xg for 30 minutes at 4°C at 10000 xg for 30 minutes at 4 °C (Beckman Coulter Optima L-80 XP Ultracentrifuge ...
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bioRxiv - Biochemistry 2020Quote: ... which were centrifuged at 50,000rpm for 4 hours at 4°C using a TLS55 rotor and an Optima MAX-XP Ultracentrifuge (Beckman Coulter). The sample was physically fractionated into 10 fractions of 85 uL each ...
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bioRxiv - Molecular Biology 2022Quote: ... 800 µl of EDTA-treated lysate was layered on top of the sucrose gradient and ultracentrifuged at 35000 rpm for 4 hours at 4 °C in a SW41Ti rotor (Beckman). After centrifugation ...
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bioRxiv - Immunology 2020Quote: ... all CCMV CP preparations were purified two times using an ultracentrifuge and 30% sucrose cushion – first with additional 0.5% Triton X-100 and the second time without Triton X-100 (4 h, 50 000 rmp, 4°C; Type 70Ti rotor, Beckman) and the pellet was then dissolved in buffer A ...
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bioRxiv - Cell Biology 2022Quote: ... 4–40% sucrose gradient prepared in lysis buffer and centrifuged at 150,000 × g for 4 or 16 h at 4°C in a MLS-50 rotor (Beckman Coulter). Thereafter ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were filtered with a membrane pore size of 0.2 μm and centrifuged at 120,000 × g for 4 h at 4 °C in polycarbonate bottle assemblies (Beckman coulter, 355603) in a 70.1 Ti fixed angle rotor (Beckman coulter ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C in a TLA 100.3 rotor (Beckman). P13 ...
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bioRxiv - Cell Biology 2022Quote: ... 4°C (Type 45 Ti rotor, Beckman Coulter) for 30 min to collect supernatant ...
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bioRxiv - Biochemistry 2020Quote: ... 4 °C (Beckman Coulter Optima LE-80K Ultracentrifuge). The clarified lysate was placed in a beaker on a stir plate at 4 °C and concentrated ammonium sulfate (5 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2019Quote: ... 4°C (Optima™ MAX Ultracentrifuge, Beckman Coulter) to sediment amorphous aggregates ...
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bioRxiv - Neuroscience 2020Quote: ... for 4 h with SW28 rotor (Beckman Coulter). The concentrated vector was stored at −80 °C until use ...
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bioRxiv - Biochemistry 2022Quote: ... 4 °C on a Sorvall WX90 ultracentrifuge (Beckman) with SW55Ti rotor ...
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bioRxiv - Molecular Biology 2022Quote: ... 4°C in SW-41Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Plant Biology 2021Quote: ... 4 °C for 18 h (Beckman SW41 rotor). The sugar solution contains 0.65 M sucrose ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C using a SW41Ti rotor (Beckman Coulter). A BioComp fractionator was used to collect fractions 1 – 16 ...
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bioRxiv - Neuroscience 2022Quote: ... at 4°C in a Beckman ultracentrifuge (Beckman). After centrifugation ...
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bioRxiv - Systems Biology 2022Quote: A particle size analyzer (Multisizer 4, Beckman Coulter) was used to count the number of cells ...
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bioRxiv - Immunology 2021Quote: ... including PE-Cy7-conjugated mouse anti-human CD45 (Beckman Coulter, CA, USA), efluor660-conjugated rat anti-human OCT3/4 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... while human B cells were CD38+/- (Beckman Coulter #6699531; 1µl per test) and CD19 (BD #557791 ...
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bioRxiv - Developmental Biology 2020Quote: ... The cells were filtered through 30 µm Pre-Separation Filters (MiltenyiBiotec #130-041-407) to obtain single cell suspension for fluorescence-activated cell sorting (MoFLo XDP, Beckman Coulter). The mouse female germ cells at each time point were collected after MoFLo XDP cell sorting as eGFP-positive fraction.
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bioRxiv - Immunology 2021Quote: Viable cells from the single cell suspension or enriched lymphocytes were counted using trypan blue exclusion with Vi CELL XR cell Viability Analyzer (Beckman Coulter). Initially ...
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bioRxiv - Microbiology 2020Quote: ... by using the single cell sorting option at a pressure of 60 psi on a MoFlo Astrios Flow Cytometer (Beckman Coulter) equipped with a 70 µm jet in air nozzle ...
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bioRxiv - Systems Biology 2020Quote: ... Single clones of HEK293 cells expressing EPO or GFP were generated from the polyclonal batches by seeding single cells per well of 384-well plates by either limited dilution or FACS (Astrios, Beckman Coulter). In case of GFP-expressing cells ...
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bioRxiv - Cell Biology 2022Quote: ... cells were again pretreated with 10μm Y-27632 for 3h before GCDR was used to achieve single cell suspension and GFP+ cells were isolated using a MoFlo Legacy cell sorter (Beckman Coulter). Sorted GFP+ single cells were sparsely replated at 1×104 cells/well in a 10cm Matrigel coated dish to facilitate clonal outgrowth ...
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bioRxiv - Cancer Biology 2021Quote: ... EGFP positive cells were single cell sorted via fluorescence activated cell sorting into 96 well plates using either an Astrios Cell Sorter (Beckman Coulter) or a BD Diva sorter (BD) ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cells were flow sorted into single wells of 96-well plates using the MoFlo XDP100 cell sorter for ATTO550 positivity (Beckman Coulter) (University of Colorado Anschutz Medical Campus Cancer Center Flow Cytometry Core) ...
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bioRxiv - Microbiology 2022Quote: ... The amplified libraries were subjected to a single left-sided bead purification using the AMPure XP magnetic beads (Beckman Coulter, Germany). Libraries were sequenced on SP lane of NovaSeq 6000 System (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... The single muscle cell suspension was sorted by tdTomato+DAPI-(ECs) and tdTomato-DAPI- (non-ECs) using MoFlo Astrios (Beckman Coulter). The sorted cells were loaded into a 10x Genomics microfluidics chip and encapsulated with barcoded oligo-dT– containing gel beads using the 10X Genomics Chromium controller according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting cell nuclei were gated for G1 phase (as determined by Hoechst and PI staining) and single nuclei were sorted into wells of 96-well plates on a MoFlo Astrios cell sorter (Beckman Coulter). 96 well plates containing nuclei and freezing buffer were stored at –80°C until further processing ...
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bioRxiv - Cell Biology 2023Quote: ... Resulting single nuclei of G1 phase (as determined by Hoechst and PI staining) were sorted into single wells of 96 wellplates on a MoFlo Astrios cell sorter (Beckman Coulter) and sorted at −80 degrees until further processing ...
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bioRxiv - Cancer Biology 2022Quote: ... The muscle ECs (CD31+CD45-DAPI-) from single cell suspension of total muscle cells were obtained by FACS sorting using MoFlo Astrios (Beckman Coulter) or also isolated using mouse CD31 microbeads (130-097-418 ...
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bioRxiv - Genomics 2023Quote: ... The remaining PCR product of the condition that displayed a single-band or near single-band product on the gel was double size-selected and purified using AMPure XP beads (Beckman coulter) and eluted in 30 μL of TE ...
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bioRxiv - Microbiology 2023Quote: ... The remaining PCR reaction of samples treated with a single gRNA was subjected to purification with AMPure XP reagent (Beckman Coulter) according to the manufacturer’s manual using AMPure beads ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were trypsinized 3 or 6 days later to make single cell suspensions and were counted using a Vi-Cell XR Cell Counter (Beckman Coulter). Cell counts were then normalized to the number of cells plated at day 0.
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bioRxiv - Neuroscience 2021Quote: ... 4 × 5-s bursts) and subsequently centrifuged at 48,000g at 4°C (Beckman Avanti J-251 Ultracentrifuge; Beckman Coulter, Fullerton, CA) for 30 min ...
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bioRxiv - Immunology 2021Quote: ... were incubated with monoclonal antibodies at 4°C for 30 min and then fixed with 4% paraformaldehyde before analysis on a Cytoflex cytometer (Beckman Coulter). Data were analyzed by Flow Jo software v.10.7 (BD Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: ... and loaded onto of a step-wise sucrose gradient as described by the manufacturer and subjected to ultracentrifugation at 150,000 xg for 4 h at 4 °C using SW50.1 rotor (Beckman Coulter, Mississauga, ON). Fractions were then collected and subject to denaturation with Laemmli buffer until further use.
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was filtered with a membrane pore size of 0.2 μm (Whatman) and centrifuged at 120,000 × g for 4 h at 4 °C in polycarbonate bottle assemblies (Beckman coulter, 355603) in a 70.1 Ti fixed angle rotor (Beckman coulter ...
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bioRxiv - Cell Biology 2020Quote: ... Protein aggregates were pelleted by ultracentrifugation of the proteins individually at 80,000 rpm in TLA 100 (Beckman Coulter) for 15 minutes ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was isolated from all TP53-KO minor cells treated in F10 matrigel assays using the Agencourt DNAdvance Genomic DNA Isolation Kit (Beckman Coulter, Cat. No. A48705), and the TP53 locus was amplified using the following primers ...
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bioRxiv - Cancer Biology 2020Quote: DNA preparation: 200 ng genomic DNA was purified using Agencourt AMPure XP Reagent (Beckman Coulter, Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... recombinant protein and anti-CD14 antibody (Beckman) were used at 20 μg/ml ...
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bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA was sequenced by Beckman Coulter Genomics (Grenoble ...
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bioRxiv - Molecular Biology 2019Quote: ... and centrifuged at 30,000 rpm for 4 h at 4°C using an SW-40 rotor in the Optima L-90K Ultracentrifuge (Beckman Coulter, USA). To identify resultant density gradient fractions containing virus particles ...