Labshake search
Citations for Beckman :
451 - 500 of 707 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: Cells were washed 1× with FACS buffer after staining and acquired on a CytoFLEX S cytometer (Beckman Coulter).
-
bioRxiv - Cell Biology 2023Quote: ... 10% and 6% and ultracentrifuged at 187,000xg for 1:30h at 4°C in Sw32.1Ti rotor (Beckman Coulter). Afterwards ...
-
bioRxiv - Biophysics 2023Quote: ... 1 mM DTT) and spun at 32,600 × g for 15 h at 4° in SW32 rotor (Beckman Coulter). Ribosome fractions were pooled as shown in Figure 2D ...
-
bioRxiv - Genomics 2023Quote: ... The DNA was purified from the lysated using a 0.9:1 ratio of Ampure XP Beads (Beckman Coulter) and eluted into 10 μl of water ...
-
bioRxiv - Developmental Biology 2023Quote: ... Peptide supernatant was cleared by ultracentrifugation at 100,000□g for 1 hour at 4□°C (Beckman Coulter, 343775), then vacuum-dried overnight.
-
bioRxiv - Biophysics 2023Quote: ... the supernatant was layered onto PBS/1.5 M sucrose solution in sample/sucrose 4:1 ratio and ultracentrifuged at 264,000 × g on Optima TLA 100.3 ultracentrifuge (Beckman for 2 h at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... CD27 and BA.1 or BA.2 were sorted using a MoFlo Astrios EQ Cell Sorter (Beckman Coulter). FACS data were collected by Summit 6.0 (Beckman Coulter ...
-
bioRxiv - Biochemistry 2024Quote: ... The mixture was centrifuged at 55,000 rpm for 1 hr in Optima™ MAX-TL Ultracentrifuge (BECKMAN COULTER). Then the pellet was dissolved with 200 μL 1X Sample buffer ...
-
bioRxiv - Immunology 2024Quote: ... Cells were then washed with 1×PBS and analyzed using a CytoFLEX Flow Cytometer (Beckman Coulter, Miami, FL) or sorted on a BD FACS Aria II ...
-
bioRxiv - Cancer Biology 2024Quote: ... followed by washing with 1 ml of PBS before the analysis on the CytoFLEX LX (Beckman Coulter, Indianapolis). Antibodies used are the following ...
-
bioRxiv - Genomics 2024Quote: ... Target sequences were then amplified using specific primers (Table 1) and purified using AMPure beads (Beckman Coulter; A63880) at concentrations appropriate to each of the target sizes ...
-
bioRxiv - Immunology 2021Quote: ... SSM liquid culture was incubated for 24 hours at 36°C with constant shaking at 180 rpm until reaching OD600nm 0.5 with 1 cm path width (Beckman Coulter™ DU 530 UV Vis spectrophotometer) ...
-
bioRxiv - Genomics 2020Quote: ... The sequencing libraries were cleaned up using AMPure XP beads (1:1.8 sample vol. to beads vol.; Beckman Coulter). The size distribution of the libraries were determined by Bioanalyzer (Agilent Technologies) ...
-
CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... diluted 5-fold in buffer A supplemented with 1 mM phenylmethane sulfonyl fluoride (PMSF) and centrifuged 40,000 rpm (45 Ti rotor, Beckman) for 60 min at 4°C to pellet vesicles ...
-
bioRxiv - Molecular Biology 2019Quote: ... The 80S fraction was pelleted for 1 h at 436000 x g at 4 °C (TLA-100, Beckman Coulter), resuspended in 20 mM HEPES pH 7.4 ...
-
bioRxiv - Biochemistry 2021Quote: ... and resuspended in 500 μL of 1% FBS/DPBS and transferred to polystyrene tubes for flow cytometric analysis (Beckman Coulter HyperCyAn ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in p24 primary antibody solution (1:100 dilution of p24-FITC antibody clone KC57 (Beckman Coulter) diluted in perm/wash buffer ...
-
bioRxiv - Biophysics 2020Quote: ... and centrifuged at 287,582 x g (90,000 rpm) for 1 hr at 4 °C in a TLA100.2 rotor (Beckman, ref. 362046) in 11×34 mm thick-wall polycarbonate ultracentrifuge tubes (Beckman ...
-
bioRxiv - Neuroscience 2022Quote: ... The samples were separated through ultracentrifugation for 1 hr at 200,000 g (spin 3 at 49,000 r.p.m. in Beckman TLS-55 rotor). Top layers of supernatants were removed ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with the fluorescently labeled anti-p24 antibody (Clone: KC57, Beckman Coulter, 6604667; 1/200 dilution).
-
bioRxiv - Biochemistry 2022Quote: ... 1 mM DTT using a MLA-50 rotor in an Optima MAX-XP Ultracentrifuge (Beckman Coulter, Pasadena, California, USA) at 180.000 g for 5 h at 4 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... The crude extract was centrifuged at 200,000 g for 1 h at 4 °C (Beckman Coulter Optima LE-80K). The membrane pellet was homogenized and solubilized in 20 mM HEPES (pH 7.8 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Amplicons from up to 3,072 libraries were pooled and purified with Ampure XP beads (0.8:1 ratio beads to product; Beckman Coulter). These steps were performed using Biomek FxP (Beckman Coulter) ...
-
bioRxiv - Microbiology 2021Quote: ... buoyant density of 1.71 g ml−1) in polyallomer tubes before sealing and ultracentrifugation at 152,000 × g (50,000 rpm) in a MLN80 rotor (Beckman-Coulter) for 72 h at 25°C ...
-
bioRxiv - Developmental Biology 2019Quote: ... Cells were refixed in 1% PFA/PBS and analyzed using a CyAn ADP Flow Cytometer (Beckman Coulter, Miami, FL). Data were collected using a four-decade log amplifier and stored in list mode for subsequent analysis using FlowJo Software (Tree Star ...
-
bioRxiv - Microbiology 2019Quote: ... The concentrated vesicles were washed twice with PBS by ultracentrifugation at 110,000xg at 4°C for 1 hour (Beckman Optma MAX-XP Ultracentrifuge ...
-
bioRxiv - Genomics 2019Quote: ... All extractions from each respective fragment section were then pooled into two samples (pooled sample 5_27-10 and pooled sample 6_2-1) and re-purified using AMPure XP Beads (Agencourt, Beckman Coulter) adding 0.4 volume (V/V ...
-
bioRxiv - Microbiology 2021Quote: ... cells were diluted in 50 mM filtered TrisHCl pH 7.5 to 106 mL-1 and analysed using a cytofluorimeter (CytoflexS, Beckman) with excitation and emission wavelength of 488 and 525 nm respectively ...
-
bioRxiv - Biochemistry 2021Quote: ... Reaction mixtures were incubated for 30 minutes and centrifuged at 100,000g at 4°C for 1 hr using a TLA100 fixed angle rotor (Beckman). The supernatant fraction was carefully removed ...
-
bioRxiv - Molecular Biology 2019Quote: After depletion of the gene of interest samples were purified using a 1 X volume XP beads (Beckman Coulter), quantified and subsequently subjected to 75-bp-single-end sequencing on the Illumina Nextseq 500 platform.
-
bioRxiv - Neuroscience 2019Quote: ... Then the lysates were spin down at 67,000 rpm for 1 hour at 18 °C with the ultracentrifuge (Beckman). The AAV particles were collected by aspirating the interface between 40-60% iodixanol with a syringe ...
-
bioRxiv - Immunology 2020Quote: ... 10 mM Tris-HCl, pH 8.1), chromatin was eluted (1% SDS, 0.1M NaHCO3)and recovered with Agencourt AMPure XP beads (Beckman Coulter). Bound chromatin and input DNA were treated with RNase H (New England Biolabs ...
-
bioRxiv - Biochemistry 2019Quote: ... and centrifuged at 287,582 x g (90,000 rpm) for 1 hr at 4°C in a TLA100.2 rotor (Beckman, ref. 362046) in 11×34 mm thick-wall polycarbonate ultracentrifuge tubes (Beckman ...
-
bioRxiv - Cell Biology 2019Quote: ... F-actin was pelleted by ultracentrifugation for 1 h at room temperature at 45,000 rpm (Beckman TLA-55 rotor) and re-suspended in ice cold G-buffer ...
-
bioRxiv - Cancer Biology 2022Quote: ... resuspended in 250 µl FACS buffer with 1 µl DAPI and analyzed with a Gallios flow cytometer (Beckman Coulter)
-
bioRxiv - Cell Biology 2022Quote: ... were mixed 1:1 with liposomes (5 mg/ml) and floated for 2h at 55,000 rpm in a TLS-55 rotor (Beckman) at 25 °C through a sucrose gradient ...
-
bioRxiv - Microbiology 2022Quote: ... 1 mM dithiothreitol and 100 μg/ml cycloheximide) and spun for 1 h at 100,000 rpm using TLA-110 rotor (Beckman) at 4 °C ...
-
bioRxiv - Microbiology 2022Quote: ... the cross-linked material was loaded onto glycerol-tartrate gradients as described elsewhere 53 and centrifuged for 1 h at 25,000 rpm in an SW-40 rotor (Beckman), with brakes set at the slowest possible deceleration ...
-
bioRxiv - Microbiology 2022Quote: ... The post-nuclear supernatant was adjusted to 0.8 M sucrose and loaded in the middle of a step gradient ranging from 0.1 to 1.8 M sucrose in STKM buffer and centrifuged at 50’000 g for 1 h at 4°C in a TLA-120.1 fixed-angle rotor (Beckman Coulter). LDs formed a white cushion on top of the tube and were collected by aspiration of the upper fraction of the gradient (125 µl).
-
bioRxiv - Neuroscience 2022Quote: ... Then the lysates were spin down at 67,000 rpm for 1 hour at 18 °C with the ultracentrifuge (Beckman). The AAV particles were collected by collecting the interface between 40 and 60% iodixanol with a syringe ...
-
Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... Individual compounds (1 µM in 0.1% DMSO) were added to each well using a Biomek NX workstation (Beckman-Coulter)(1 well per compound) ...
-
bioRxiv - Biophysics 2023Quote: ... The supernatant was subjected to a second round of ultracentrifugation at 100,000 x g for 1 hour at 4°C in a Type 45Ti rotor (Beckman) to pellet cell membranes ...
-
bioRxiv - Plant Biology 2023Quote: ... It was then centrifuged again at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter ...
-
bioRxiv - Cell Biology 2023Quote: ... and 1 mM PMSF) and centrifuged for 15 hours at 4°C with 74,400 xg (SW 32.1 Ti; Beckman Coulter). 24 equal volume fractions were collected from the top and subjected to TCA precipitation ...
-
bioRxiv - Plant Biology 2023Quote: ... The tubes were then centrifuged at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter ...
-
bioRxiv - Immunology 2023Quote: ... SSM liquid culture was incubated for 24 hours at 36°C with constant shaking at 180 rpm until reaching mid-log phase OD600nm 0.5 with 1 cm path width (Beckman Coulter™ DU 530 UV Vis spectrophotometer) ...
-
Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The gradients then were centrifuged at 35,000 rpm (210,000g) for 1 h at 4°C with no brake in an SW41Ti rotor (Beckman Coulter). Two samples were collected from the nucleus gradient ...
-
bioRxiv - Neuroscience 2023Quote: ... the supernatant was collected and applied for further centrifugation at 40,000 rpm for 1 h at 4°C (45 Ti fixed-angle rotor, Beckman) to pellet the membranes ...
-
bioRxiv - Neuroscience 2023Quote: ... NP-40 prepared in DPBS] and then ultracentrifuged at 100,000x g for 1 h at 4 °C using a TLA-55 rotor (Beckman). The supernatant was removed ...
-
bioRxiv - Developmental Biology 2023Quote: ... Size selection and purification was achieved by double-sided (0.65 × −1× volume) using SPRIselect magnetic beads (Beckman Coulter, B23318). Concentrations were measured using the Qubit HS dsDNA assay ...