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Citations for Beckman :
51 - 100 of 1744 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Protein-liposome complexes were separated from unbound protein by centrifugation (2 h at 45.000 rpm, 4 °C, MLS50 rotor -Beckman Coulter) in the density gradient ...
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bioRxiv - Synthetic Biology 2019Quote: ... Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 32 Ti) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were centrifuged at 135,000 × g for 1 hr at 4°C (Beckman OptimaTM Max centrifuge ...
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bioRxiv - Genomics 2023Quote: ... cellular/protein debris were then collected by centrifugation at 1,000xg for 15 minutes at 4°C (Beckman Coulter Allegra X-14R), and the supernatant (DNA ...
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bioRxiv - Microbiology 2023Quote: ... Virus was separated from free dye by ultracentrifugation as above on a SW60Ti rotor (Beckman Coulter). The virus band was extracted and desalted on a NAP column (VWR ...
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bioRxiv - Biochemistry 2021Quote: ... 1□mM EDTA) and centrifuged at 25000 rpm for 1 hour at 4°C (Beckman Coulter SW41-Ti rotor). Mitochondria forming a band at the interphase between the 1 M and 1.5 M sucrose were collected and resuspended in 10 mM Tris-HCl pH = 7.5 in 1:1 ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 hour at 37°C and purified with RNAClean XP beads (Beckman Coulter). The RNA ends were extended using E ...
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bioRxiv - Biophysics 2023Quote: ... and at 4 °C for 1 hour for ultracentrifugation (70,658 g, Beckman, Type 70Ti) in vacuum ...
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bioRxiv - Plant Biology 2023Quote: ... at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor, Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... for 1 hour at 4°C in a Type 45 Ti rotor (Beckman Coulter). The clarified lysate was then incubated with 5 mL of Ni-NTA agarose beads (Qiagen) ...
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bioRxiv - Developmental Biology 2023Quote: ... cleared by ultracentrifugation at 100,000Lg for 1 hour at 4□°C (Beckman Coulter, 343775), then and vacuum-dried overnight.
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bioRxiv - Microbiology 2024Quote: ... 4°C for 1 h in a SW 41 Ti rotor (Beckman Coulter, Inc). The pellet was resuspended in 1 ml of PBS pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... were pre-clarified to remove aggregate proteins by spinning at 65,000 rpm for 30 min at 4°C in a TLA100 rotor (Beckman Coulter, Brea, CA) before incubating with F-actin ...
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bioRxiv - Cell Biology 2019Quote: ... The obtained supernatant (containing membrane and cytosolic proteins) was then centrifuged at 180,000 × g for 90 min at 4°C using a TLA-55 rotor (Beckman Coulter, Brea, CA). The obtained pellet (containing intact membranes ...
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bioRxiv - Synthetic Biology 2021Quote: ... 8 °C (Beckman Coulter Optima MAX-XP ...
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bioRxiv - Synthetic Biology 2021Quote: ... 8 °C (Beckman Coulter Optima MAX-XP ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at 37°C before purification with 42 μl AMPure beads (Beckman Coulter). The sample was eluted in 20 μl water which served as input for sequencing library generation ...
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bioRxiv - Developmental Biology 2023Quote: ... and centrifuged at 40,000 rpm for 1 h at 4°C (Beckman SW-41Ti rotor). Supernatants were discarded ...
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bioRxiv - Developmental Biology 2023Quote: The anti–human uncoupled primary antibodies used are listed: anti-human CD34 (Beckman, QBEnd/10), anti-human ACE (BB9 ...
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bioRxiv - Biochemistry 2021Quote: ... The virus-containing filtrate was then further purified using an ultracentrifuge (Optima XPN-90 Ultracentrifuge, Beckman Coulter, USA) with a cycle of 1,000 rpm (116 g ...
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bioRxiv - Molecular Biology 2022Quote: ... 4°C (Beckman Coulter). The pellet containing lentiviral particles was suspended in the residual medium ...
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bioRxiv - Genomics 2020Quote: ... 4°C (Beckman Coulter). Supernatant was loaded onto 5 ml HisTrap FF columns (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C (Beckman Coulter). Clarified lysates were poured several times over 0.5-1 ml Streptactin resin (IBA ...
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bioRxiv - Cancer Biology 2023Quote: ... 4°C (Beckman Coulter). The pellet containing lentiviral particles was suspended in the residual medium ...
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bioRxiv - Cell Biology 2020Quote: ... and then the sample was centrifuged at 182,000g for 1 h at 4°C (Beckman SW40). LD fraction was carefully collected from the top band of the gradient and washed three times using 200 μL of buffer B ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated for 1 hr at +4°C before the flow cytometry analysis (Beckman Coulter CytoFlex) or sorting (SONY MA900) ...
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bioRxiv - Immunology 2021Quote: ... VLPs were concentrated using ultracentrifuge (TLA100.3 rotor, Beckman; at 72,000 rpm for 1 hour, +5°C) or by ultrafiltration using Amicon Ultra 15 (100 kDa ...
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bioRxiv - Molecular Biology 2022Quote: ... for 1 additional hour at 55°C and purified using 2x AMPure XP beads (Beckman Coulter). The generated libraries were amplified using the KAPA HiFi Hotstart Ready Mix and Nextera Index Kit 1 (i7 ...
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bioRxiv - Biochemistry 2023Quote: ... The crude lysate was centrifuged for 1 hour at 20,000 rpm and 4 °C (Beckman Coulter). The supernatant was filtered using a 0.44 μm syringe filter (Sartorius ...
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bioRxiv - Biophysics 2023Quote: ... The cell lysate was centrifuged at 4 °C at 35000 rpm for 1 hour by Beckman Optima™ L-80XP ultracentrifuge equipped with a Ti-45 rotor (Beckman ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 18 ◦C for 1 h 45 min in a 50.2Ti rotor (Beckman-Coulter, Brea, CA, USA). After ultracentrifugation ...
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bioRxiv - Immunology 2022Quote: ... anti-human TCR Vβ1 (Beckman Coulter), anti-human CD3 and CD8 antibodies (BD Bioscience).
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bioRxiv - Immunology 2020Quote: ... Plate was then incubated 1 h à 37 °C with 1 μg per well of alkaline phosphatase-coupled anti-Igκ or anti-IgM (Beckman Coulter) in PBS ...
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bioRxiv - Microbiology 2020Quote: ... The virus was then purified over a 20% sucrose cushion by centrifugation at 35,000 rpm (Beckman Coulter SW41 rotor) for 3 h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Virus particles in the supernatant were pelleted by ultracentrifugation at 45.000 rpm for 45 min (Beckman 70.1 Ti Rotor) over a pad of 15% sucrose in 10 mM Tris-HCl buffer (pH 7.4) ...
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bioRxiv - Microbiology 2024Quote: ... Virus RNA was extracted with a magnetic bead-based RNA extraction kit (RNAdvance Viral Reagent kit, Beckman Coulter, C63510) on an epMotion® 5073t liquid handler (Eppendorf ...
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bioRxiv - Immunology 2022Quote: Human Vγ9Vδ2 T lymphocytes were stained with FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and APC-labeled anti-human CD3ε mAb (#UCHT1 ...
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bioRxiv - Molecular Biology 2022Quote: ... and centrifuged at 100,000 g for 1 h at 4°C in a TLA100.3 rotor (Beckman Coulter). Top (T ...
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bioRxiv - Neuroscience 2021Quote: ... it was centrifuged at 110 000×g for 1 hour at 4°C (Optima TLX Ultracentrifuge, Beckman) and the fat-free infranatant was used for HSL activity measurements ...
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bioRxiv - Physiology 2023Quote: ... and centrifuged at 12,000g for 1 hour at 4°C (SW-41Ti rotor, Beckman Coulter, Pasadena, California). Six or seven fractions were collected from the top ...
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bioRxiv - Biochemistry 2023Quote: ... the solution was ultracentrifuged at 91,000 g at 4 °C for 1 hour (Optima TLX Ultracentrifuge, Beckman). The concentration of the supernatant was then determined by A280 (ε280 = 5,960 M−1 cm−1) ...
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bioRxiv - Biochemistry 2024Quote: ... After centrifugation for 1 hour at 55000 rpm and at 20°C in a TLS 55 (Beckman) rotor ...
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bioRxiv - Cancer Biology 2023Quote: ... at 37°C for 1 hour and purified using Agencourt AMPure XP beads (0.8X) (Beckman Coulter, U.S.). For second strand synthesis ...
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bioRxiv - Cell Biology 2024Quote: ... The tubes were then centrifuged at 200,000 g for 1 h at 22 °C (TL 100, Beckman). After ultracentrifugation ...
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bioRxiv - Biophysics 2023Quote: ... Virus-containing supernatants were collected at 48 hours and 72 hours after transfection and concentrated using ultracentrifugation (Ultraclear, Beckman 344058). The transduction capacity of the viral vector product was confirmed by culturing non-transfected HEK293T cells with the virus after 24 hours and assessing via fluorescent imaging.
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bioRxiv - Biochemistry 2019Quote: ... 4 °C (Beckman JLA 16.2500) and then bound to 0.1 cell paste volumes of anti-FLAG resin (Sigma M2 ...
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bioRxiv - Biochemistry 2019Quote: ... 4 °C (Beckman JLA 8.1000), washed in pre-chilled PBS and resuspended in 20 cell paste volumes of preparation buffer (50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C (SW55Ti rotor, Beckman). The pellets were resuspended in 25% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C (VTi65.1 rotor, Beckman) with the maximum acceleration and minimum deceleration allowing for isopycnic separation of subcellular particles and membranes ...
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bioRxiv - Biochemistry 2021Quote: ... 4°C (SW41 rotor, Beckman). The supernatant was concentrated with a 100-kDa molecular weight cutoff concentrator (Merck Millipore ...