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Citations for Beckman :
1 - 50 of 109 citations for Hepatitis E Virus Capsid Protein ORF2 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The amount of p24 capsid protein was determined by the HIV-1 p24 ELISA antigen assay (Beckman Coulter, Fullerton, CA). Virus from different productions averaged 175 ng/µl of p24 antigen.
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bioRxiv - Microbiology 2022Quote: ... and C capsids were separated by sedimentation at 50,000 x g and 4°C for 80 min (SW40Ti, Beckman Coulter) on linear 20 to 50% sucrose gradients in TKE buffer (20 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... spinning at 3,200 rpm (Beckman J-6M/E centrifuge). The centrifuge chamber was set at 4°C during all centrifugation steps until approximately 10 min before cell harvesting ...
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bioRxiv - Microbiology 2024Quote: ... The virus was enriched by ultracentrifugation (Beckman Coulter Optima™ L-80XP) at 246,347g for 4 hours at 4 °C ...
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bioRxiv - Genomics 2021Quote: Beckman Coulter Avanti J-E centrifuge (Beckman Coulter, cat, no. A20698)
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bioRxiv - Genomics 2019Quote: ... the Hi-C libraries were purified with AMPure XP beads (Beckman Coulter). The concentration of the Hi-C libraries was determined by Bioanalyzer profiles (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... the Hi-C libraries were purified with AMPure XP beads (Beckman Coulter). Quantity and integrity of the Hi-C libraries was determined by Bioanalyzer profiles (Agilent Technologies).
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bioRxiv - Microbiology 2020Quote: ... the virus particles were pelleted by ultracentrifugation in a Beckman type 19 rotor (Beckman Coulter ...
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bioRxiv - Synthetic Biology 2020Quote: Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 28) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor) for 2h at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... and spun for 1 hour in a MAX-E tabletop ultracentrifuge (Beckman) at 4°C and 100,000 × g (47,000 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... The lysed cells were pelleted via centrifugation (Beckman Coulter, Avanti J-E) at 40,000 × g for 35 min ...
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bioRxiv - Synthetic Biology 2019Quote: ... Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 32 Ti) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Genetics 2019Quote: ... The Hi-C libraries were purified with AMPure XP beads (Beckman Coulter, A63881) and sequenced using an Illumina sequencing platform.
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bioRxiv - Microbiology 2023Quote: ... Virus was separated from free dye by ultracentrifugation as above on a SW60Ti rotor (Beckman Coulter). The virus band was extracted and desalted on a NAP column (VWR ...
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bioRxiv - Biochemistry 2022Quote: ... Packed eggs were crushed by centrifugation (BECKMAN, Avanti J-E, JS13.1 swinging rotor) for 20 min at 18,973×g ...
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bioRxiv - Molecular Biology 2020Quote: ... Packed eggs were crushed by centrifugation (BECKMAN, Avanti J-E, JS13.1 swinging rotor) for 20 min at 18973 × g ...
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bioRxiv - Biochemistry 2023Quote: ... The lysed cell contents were pelleted via centrifugation (Beckman Coulter, Avanti J-E) at 40,000 × g for 35 min ...
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bioRxiv - Biochemistry 2024Quote: Packed eggs were crushed by centrifugation (BECKMAN, Avanti J-E, JS13.1 swinging rotor) for 20 min at 18,973×g ...
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bioRxiv - Genetics 2022Quote: ... and the final Hi-C library was purified with AMPure XP beads (Beckman Coulter). Promoter Capture Hi-C was performed using a customdesign Agilent SureSelect system following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... and Hi-C template DNA was purified using 1x AMPure XP beads (Beckman Coulter, A63881) and stored at 4°C until library preparation ...
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bioRxiv - Cancer Biology 2024Quote: ... 10,000 × g for 30 min) at 4°C (Beckman Coulter Avanti J-E Centrifuge); filtered through a 0.22 μm PES filter (Genesee) ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibody derived tags (ADTs) and cDNA were separated using the Ampure Beads (Beckman Coulter, USA) and constructed libraries dependently ...
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bioRxiv - Biochemistry 2021Quote: ... The virus-containing filtrate was then further purified using an ultracentrifuge (Optima XPN-90 Ultracentrifuge, Beckman Coulter, USA) with a cycle of 1,000 rpm (116 g ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 18000 rpm for 30 minutes (Beckman Coulter Avanti J-E) and the supernatant was used for further purification ...
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bioRxiv - Molecular Biology 2022Quote: ... Eggs were packed into tubes by centrifugation (BECKMAN, Avanti J-E, JS-13.1 swinging rotor) for 1 min at 190 xg and crushed by centrifugation for 20 min at 18,973 xg ...
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bioRxiv - Microbiology 2020Quote: ... Samples were centrifuged at 6000 RPM for 10 mins (Avanti-J-E HPC, Beckman, USA), and the pellet was resuspended in an equal volume of isolation buffer (50 mM 3-(N-morpholino ...
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bioRxiv - Biochemistry 2022Quote: ... Eggs were packed into tubes by centrifugation (BECKMAN, Avanti J-E, JS-13.1 swinging rotor) for 1 min at 190×g and crushed by centrifugation for 20 min at 18,973×g ...
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bioRxiv - Cancer Biology 2024Quote: ... and spinning at 12,535 g for 8 s (Beckman E centrifuge, Beckman Coulter, Fullerton, CA). The tube was placed briefly in liquid nitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The virus was then purified over a 20% sucrose cushion by centrifugation at 35,000 rpm (Beckman Coulter SW41 rotor) for 3 h at 4°C ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...
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bioRxiv - Molecular Biology 2020Quote: ... Virus particles in the supernatant were pelleted by ultracentrifugation at 45.000 rpm for 45 min (Beckman 70.1 Ti Rotor) over a pad of 15% sucrose in 10 mM Tris-HCl buffer (pH 7.4) ...
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bioRxiv - Microbiology 2024Quote: ... Virus RNA was extracted with a magnetic bead-based RNA extraction kit (RNAdvance Viral Reagent kit, Beckman Coulter, C63510) on an epMotion® 5073t liquid handler (Eppendorf ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then centrifuged in an Optima MAX-E tabletop ultracentrifuge (Beckman Coulter, Brea, CA, USA) at 184,000 x g (65,000 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... the virus-cell mixture was centrifuged at 1,000 rcf for 2 hours at 32°C (Beckman Coulter, Allegra X-30R). Infected cells were then cultured as described above and subsequently FACS-sorted for the establishment of clonal cell lines.
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bioRxiv - Biophysics 2023Quote: ... Virus-containing supernatants were collected at 48 hours and 72 hours after transfection and concentrated using ultracentrifugation (Ultraclear, Beckman 344058). The transduction capacity of the viral vector product was confirmed by culturing non-transfected HEK293T cells with the virus after 24 hours and assessing via fluorescent imaging.
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bioRxiv - Microbiology 2020Quote: ... Bacterial cells were harvested by centrifugation at 5000 rpm (JLA 9.1000 rotor, Beckman Coulter Avanti J-E) for 20 min and re-suspended in 100 ml lysis buffer A (50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The cells were collected by centrifugation at 4000 rpm in an Avanti J-E centrifuge (Beckman Coulter) with a JA-14 rotor at 4°C during 30 min and pellets were frozen at -80°C until further processing.
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bioRxiv - Systems Biology 2021Quote: HeLa Kyoto and R1/E mESCs (gifts from Hyman lab, Dresden) and HEK293 (gift from Beckman lab, Berlin), Ptk2 cell lines (gift from Simons lab ...
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bioRxiv - Microbiology 2021Quote: 1 mL pseudoparticles were pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by centrifugation at 15,500 x g for 25 min in a JA 25.50 rotor in an Avanti J-E centrifuge (Beckman). The supernatant was transferred to a 50-mL conical tube ...
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bioRxiv - Molecular Biology 2022Quote: ... Bacteria were harvested at 4,000 x g for 25 min in a JLA-8.1000 rotor in an Avanti J-E centrifuge (Beckman), and the pellet was transferred to a 50-mL conical tube (Sarstedt ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was collected and virus pelleted through 30% (w/v) sucrose cushion at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 hours at 10°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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bioRxiv - Biochemistry 2021Quote: ... after which the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA 25.50 rotor in an Avanti J-E centrifuge (Beckman). Nucleic acids in the clarified lysate were precipitated by slowly adding polyethyleneimine with constant stirring in an ice bath to a final concentration of 0.4% ...
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA25.50 rotor in an Avanti J-E centrifuge (Beckman). The clarified lysate was then added to 10 mL of Ni-NTA Agarose beads (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... All centrifugation steps were performed at 4 °C in an Avanti J-E high-speed fixed-angle centrifuge (Beckman Coulter Life Sciences ...
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bioRxiv - Biophysics 2020Quote: ... lysate was centrifuged at 5 °C with 13000 rpm for 15 min by Avanti J-E Ultra Centrifuge (Beckman Coulter). Supernatant from centrifugation was filtered with 0.45 μm SFCA syringe filters (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: 3 mL of pseudoparticles were pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter, Inc.) for 2 hours at 42,000 rpm at 4°C ...