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Citations for Beckman :
1 - 50 of 56 citations for Hantaan Virus Nucleoprotein E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... spinning at 3,200 rpm (Beckman J-6M/E centrifuge). The centrifuge chamber was set at 4°C during all centrifugation steps until approximately 10 min before cell harvesting ...
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bioRxiv - Microbiology 2024Quote: ... The virus was enriched by ultracentrifugation (Beckman Coulter Optima™ L-80XP) at 246,347g for 4 hours at 4 °C ...
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bioRxiv - Genomics 2021Quote: Beckman Coulter Avanti J-E centrifuge (Beckman Coulter, cat, no. A20698)
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bioRxiv - Microbiology 2020Quote: ... the virus particles were pelleted by ultracentrifugation in a Beckman type 19 rotor (Beckman Coulter ...
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bioRxiv - Synthetic Biology 2020Quote: Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 28) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor) for 2h at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... and spun for 1 hour in a MAX-E tabletop ultracentrifuge (Beckman) at 4°C and 100,000 × g (47,000 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... The lysed cells were pelleted via centrifugation (Beckman Coulter, Avanti J-E) at 40,000 × g for 35 min ...
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bioRxiv - Synthetic Biology 2019Quote: ... Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 32 Ti) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Virus was separated from free dye by ultracentrifugation as above on a SW60Ti rotor (Beckman Coulter). The virus band was extracted and desalted on a NAP column (VWR ...
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bioRxiv - Biochemistry 2022Quote: ... Packed eggs were crushed by centrifugation (BECKMAN, Avanti J-E, JS13.1 swinging rotor) for 20 min at 18,973×g ...
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bioRxiv - Molecular Biology 2020Quote: ... Packed eggs were crushed by centrifugation (BECKMAN, Avanti J-E, JS13.1 swinging rotor) for 20 min at 18973 × g ...
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bioRxiv - Biochemistry 2023Quote: ... The lysed cell contents were pelleted via centrifugation (Beckman Coulter, Avanti J-E) at 40,000 × g for 35 min ...
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bioRxiv - Biochemistry 2024Quote: Packed eggs were crushed by centrifugation (BECKMAN, Avanti J-E, JS13.1 swinging rotor) for 20 min at 18,973×g ...
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bioRxiv - Cancer Biology 2024Quote: ... 10,000 × g for 30 min) at 4°C (Beckman Coulter Avanti J-E Centrifuge); filtered through a 0.22 μm PES filter (Genesee) ...
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bioRxiv - Biochemistry 2021Quote: ... The virus-containing filtrate was then further purified using an ultracentrifuge (Optima XPN-90 Ultracentrifuge, Beckman Coulter, USA) with a cycle of 1,000 rpm (116 g ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 18000 rpm for 30 minutes (Beckman Coulter Avanti J-E) and the supernatant was used for further purification ...
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bioRxiv - Molecular Biology 2022Quote: ... Eggs were packed into tubes by centrifugation (BECKMAN, Avanti J-E, JS-13.1 swinging rotor) for 1 min at 190 xg and crushed by centrifugation for 20 min at 18,973 xg ...
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bioRxiv - Microbiology 2020Quote: ... Samples were centrifuged at 6000 RPM for 10 mins (Avanti-J-E HPC, Beckman, USA), and the pellet was resuspended in an equal volume of isolation buffer (50 mM 3-(N-morpholino ...
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bioRxiv - Biochemistry 2022Quote: ... Eggs were packed into tubes by centrifugation (BECKMAN, Avanti J-E, JS-13.1 swinging rotor) for 1 min at 190×g and crushed by centrifugation for 20 min at 18,973×g ...
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bioRxiv - Cancer Biology 2024Quote: ... and spinning at 12,535 g for 8 s (Beckman E centrifuge, Beckman Coulter, Fullerton, CA). The tube was placed briefly in liquid nitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The virus was then purified over a 20% sucrose cushion by centrifugation at 35,000 rpm (Beckman Coulter SW41 rotor) for 3 h at 4°C ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...
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bioRxiv - Molecular Biology 2020Quote: ... Virus particles in the supernatant were pelleted by ultracentrifugation at 45.000 rpm for 45 min (Beckman 70.1 Ti Rotor) over a pad of 15% sucrose in 10 mM Tris-HCl buffer (pH 7.4) ...
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bioRxiv - Microbiology 2024Quote: ... Virus RNA was extracted with a magnetic bead-based RNA extraction kit (RNAdvance Viral Reagent kit, Beckman Coulter, C63510) on an epMotion® 5073t liquid handler (Eppendorf ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then centrifuged in an Optima MAX-E tabletop ultracentrifuge (Beckman Coulter, Brea, CA, USA) at 184,000 x g (65,000 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... the virus-cell mixture was centrifuged at 1,000 rcf for 2 hours at 32°C (Beckman Coulter, Allegra X-30R). Infected cells were then cultured as described above and subsequently FACS-sorted for the establishment of clonal cell lines.
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bioRxiv - Biophysics 2023Quote: ... Virus-containing supernatants were collected at 48 hours and 72 hours after transfection and concentrated using ultracentrifugation (Ultraclear, Beckman 344058). The transduction capacity of the viral vector product was confirmed by culturing non-transfected HEK293T cells with the virus after 24 hours and assessing via fluorescent imaging.
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bioRxiv - Microbiology 2020Quote: ... Bacterial cells were harvested by centrifugation at 5000 rpm (JLA 9.1000 rotor, Beckman Coulter Avanti J-E) for 20 min and re-suspended in 100 ml lysis buffer A (50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The cells were collected by centrifugation at 4000 rpm in an Avanti J-E centrifuge (Beckman Coulter) with a JA-14 rotor at 4°C during 30 min and pellets were frozen at -80°C until further processing.
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bioRxiv - Systems Biology 2021Quote: HeLa Kyoto and R1/E mESCs (gifts from Hyman lab, Dresden) and HEK293 (gift from Beckman lab, Berlin), Ptk2 cell lines (gift from Simons lab ...
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bioRxiv - Microbiology 2021Quote: 1 mL pseudoparticles were pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by centrifugation at 15,500 x g for 25 min in a JA 25.50 rotor in an Avanti J-E centrifuge (Beckman). The supernatant was transferred to a 50-mL conical tube ...
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bioRxiv - Molecular Biology 2022Quote: ... Bacteria were harvested at 4,000 x g for 25 min in a JLA-8.1000 rotor in an Avanti J-E centrifuge (Beckman), and the pellet was transferred to a 50-mL conical tube (Sarstedt ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was collected and virus pelleted through 30% (w/v) sucrose cushion at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 hours at 10°C ...
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bioRxiv - Biochemistry 2021Quote: ... after which the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA 25.50 rotor in an Avanti J-E centrifuge (Beckman). Nucleic acids in the clarified lysate were precipitated by slowly adding polyethyleneimine with constant stirring in an ice bath to a final concentration of 0.4% ...
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA25.50 rotor in an Avanti J-E centrifuge (Beckman). The clarified lysate was then added to 10 mL of Ni-NTA Agarose beads (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... All centrifugation steps were performed at 4 °C in an Avanti J-E high-speed fixed-angle centrifuge (Beckman Coulter Life Sciences ...
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bioRxiv - Biophysics 2020Quote: ... lysate was centrifuged at 5 °C with 13000 rpm for 15 min by Avanti J-E Ultra Centrifuge (Beckman Coulter). Supernatant from centrifugation was filtered with 0.45 μm SFCA syringe filters (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: 3 mL of pseudoparticles were pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter, Inc.) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant from 2,000 × g was transferred to 30mL conical tube and then centrifuged at 10,000 × g for 10 min at 4°C (# Avanti J-E JA25-50 Beckman Coulter). The supernatant from this spin was filtered through a 0.22-μm mesh filter (# SLGP033RS EMD Millipore ...
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bioRxiv - Synthetic Biology 2019Quote: ... then centrifuged at 5000 x g and 10°C for 10 minutes (Avanti□ J-E Centrifuge, Beckman Coulter, Indianapolis, IN). After disposing the supernatant ...
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bioRxiv - Biochemistry 2022Quote: ... A soluble fraction was obtained after centrifugation of the lysate at 19,000 rpm for 40 min at 4 °C performed using Avanti J-E with a rotor JA-20 (BECKMAN COULTER) to remove insoluble debris.
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bioRxiv - Plant Biology 2024Quote: ... The mixture was then centrifuged for 20 min at 9,700 g at 6°C using an Avanti J-E centrifuge and a JLA-16.250 rotor (Beckman Coulter, USA). The supernatant was separated and centrifuged for 2.5 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge and a Type 45 Ti rotor (Beckman Coulter ...
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bioRxiv - Plant Biology 2024Quote: ... The suspension was then centrifuged for 20 min at 9,700 g at 6°C using an Avanti J-E centrifuge and a JLA-16.250 rotor (Beckman Coulter, USA). The supernatant was separated and centrifuged for 3 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge with a Type 45 Ti rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: A 3 ml volume of pseudoparticles was pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... coli cells obtained from 500 mL culture were harvested using centrifugation for 10 min at 4800 rpm (Beckman, Allegra-30XR), and were subjected to freezing in liquid nitrogen ...
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bioRxiv - Microbiology 2022Quote: ... coli treated cells were also diluted with PBS and sorted using a MoFlo XDP High-Speed Cell Sorter (Beckman Coulter) equipped with 405 nm ...
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bioRxiv - Microbiology 2020Quote: ... and the resultant lysate was transferred into SS34 tubes for further centrifugation at 18,000 rpm (JA-25.50 rotor, Beckman Coulter Avanti J-E) for 30 min ...