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Citations for Beckman :
51 - 99 of 99 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... 40 μL of fixed cells were diluted in 10 mL diluent (Isoton II; Beckman Coulter) and sonicated for 5 pulses of approximately 0.5 second each at low power ...
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bioRxiv - Bioengineering 2022Quote: ... The frequency of IFNγ positive cells was analyzed by flow cytometry (CytoFLEX II, Beckman Coulter). Dead cells were excluded from the analysis by staining with Zombie green (Biolegend) ...
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bioRxiv - Cell Biology 2021Quote: 1 mL samples of culture were diluted 1:100 with Isoton II Diluent (Beckman Coulter) and measured on a Beckman Multisizer 3 Coulter Counter to produce a histogram of the population’s cell volumes ...
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bioRxiv - Molecular Biology 2022Quote: ... sonicated and then diluted in 10-20 ml of Isoton II diluent (Beckman Coulter #8546719) before measurement.
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Phenotypic and genotypic adaptation of E. coli to thermal stress is contingent on genetic backgroundbioRxiv - Evolutionary Biology 2023Quote: ... by diluting 50 μl of overnight culture into 9.9ml of Isoton II Diluent (Beckman Coulter) and measuring cell density in volumetric mode on a Multisizer 3 Coulter Counter (Beckman Coulter) ...
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bioRxiv - Microbiology 2023Quote: ... 50 µg/ml CHX) prepared using a BioComp gradient master in ultra-clear centrifuge tubes (Beckman Coulter) and centrifuged for 2:30 hours at 36.000 rpm in an SW41 rotor ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μL of this enzyme mix was distributed in wells using a Biomek 4000 (Beckman), containing 2 μL of the compounds ...
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bioRxiv - Cancer Biology 2023Quote: ... ds cDNA was separated from second-strand reaction mix using AMPure XP beads (Beckman Coulter) resulting in blunt-ended cDNA ...
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bioRxiv - Cell Biology 2021Quote: 1 mL samples of culture were diluted 1:100 diluted with Isoton II Diluent (Beckman Coulter) and used to determine cell concentration of the culture using a Beckman Multisizer 3 Coulter Counter ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was amplified using PCR Primer II A and subsequently purified using Ampure XP beads (Beckman). Illumina libraries were prepared using Nextera XT DNA library preparation kits (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... the mix was size separated using 0.6x volume of AMPure XP magnetic beads (Beckman Coulter, USA). The short fragments (the proteomic library that was retained in the supernatant ...
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bioRxiv - Cancer Biology 2020Quote: ... equivalent amounts of ribosomes for each lysate was loaded onto each 10%-45% sucrose density gradient (BioComp Gradient Master machine) for ultracentrifugation at 33,000 rpm for 5 hours (Beckman Optima L80K Ultracentrifuge ...
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bioRxiv - Cell Biology 2021Quote: ... and then 100-150 µL was diluted into 10 mL of Isoton II diluent (Beckman Coulter #8546719), and 40,000-50,000 cells were measured per sample ...
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bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II, respectively); and βHCG and AFP CMIA-ARCHTECT I 4000.The assays were performed as recommended by the manufacturers ...
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bioRxiv - Bioengineering 2023Quote: ... by adding 10 μL of the PBMC solution to 10 mL of Isoton II diluent (Beckman Coulter) in a 20 mL cuvette ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were analysed for induction of apoptosis using a flow cytometer (BD FACSCanto™ II, Beckman Dickenson).
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bioRxiv - Biochemistry 2020Quote: ... Each of the 80 compounds were added to the reaction mix using a BioMek I5 workstation (Beckman) to a final concentration of 20 µM in 5% DMSO ...
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bioRxiv - Molecular Biology 2021Quote: ... The products were purified with the AMPure XP kit using a 1X beads:PCR mix ratio (Beckman Coulter). Pooled barcoded libraries (exponential or stationary phase ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Changes in the size and biovolume were investigated using a Coulter Counter Multisizer II (Beckman Coulter, Nyon, Switzerland) with a 50 μm orifice tube.
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bioRxiv - Neuroscience 2024Quote: ... were loaded onto 10–50% continuous sucrose density gradients in polysome buffer (20 mM HEPES pH 7.4, 150 mM KCl, 5 mM MgCl2) (Gradient Master™, BioComp) and centrifuged in an SW40Ti swing-out rotor (Beckman Coulter) at 250,000 g for 2 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... 100 µl/well of a 1:1 mix of methanol:acetonitrile were added using the Biomek FXP (Beckman Coulter) liquid handling system ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR-II product from a given sample was pooled and DNA was purified with AMPure XP beads (Beckman Coulter) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The sonicated pollen samples were filtered through 200 µm sieves using 100 ml Coulter Isoton II diluent (Beckman Coulter) to eliminate anther debris ...
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bioRxiv - Microbiology 2021Quote: ... and 500 μl of a 10 ml resuspension of cells from each flask diluted with 19.5 ml Isoton II Diluent (Beckman). PD time ...
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bioRxiv - Systems Biology 2022Quote: ... The cell culture in a 48-well microplate (Iwaki) was suspended in 10 ml of ISOTON II (Beckman Coulter). Every 100 to 500 μl of the suspended solutions were flowed for particle analysis ...
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bioRxiv - Genomics 2020Quote: ... PCR reactions of the same mix were pooled and purified by addition of 280μl AMPure XP beads (Beckman Coulter). Beads were washed twice with 800μl 80% ethanol and assembled inserts were eluted in 100μl water ...
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bioRxiv - Genomics 2021Quote: ... containing a mixture of DPBS and shearing master mix at a final volume of 2.14 μL using a MoFlo Astrios cell sorter running Summit v6.3 (Beckman Coulter). The Astrios was equipped with a 100 μm nozzle at 25 psi ...
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bioRxiv - Microbiology 2023Quote: ... Then 90 ul of the mix was transferred to a fresh tube and cleaned with SPRIselect beads (Beckman Coulter) using a two-sided (1.2x-0.5X ...
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bioRxiv - Biophysics 2024Quote: ... Each seed mix was clarified using an ultra-centrifuge (Beckman Optima MAX-XP, 90,000 RPM, 4°C, 5 min) then snap-frozen in small aliquots and stored at -80°C ...
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bioRxiv - Molecular Biology 2019Quote: ... the region was amplified using Phusion HS II DNA polymerase the amplicon was purified using AMPure beads (Beckman Coulter A63881), and sequenced to confirm the presence of mutations in vivo.
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bioRxiv - Genetics 2020Quote: ... The data were acquired within an hour on a BD FACS LSR II flow cytometer (Beckman Coulter Life Sciences, USA).
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bioRxiv - Bioengineering 2022Quote: ... The frequency of proliferating T cells was determined after 96 hours by flow cytometry using a CytoFLEX II (Beckman Coulter). Zombie Green (Biolegend ...
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bioRxiv - Genetics 2022Quote: ... Plugs were digested with the restriction enzyme NotI and electrophoretically separated by the pulsed-field methods of either transverse alternating field electrophoresis (TAFE, Geneline II, Beckman) as previously described (Gralton et al ...
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bioRxiv - Plant Biology 2023Quote: ... and all granules purified within this fraction was resuspended in known volumes of Isoton II electrolyte solution (Beckman Coulter, Indianapolis). The suspension was analysed in a Multisizer 4e Coulter counter (Beckman Coulter ...
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bioRxiv - Genomics 2019Quote: ... double stranded cDNA for each 96-well quadrant and used 0.8x volume of SPRI beads mix that contained 76.6 µl of the original RNAClean XP SPRI beads (Beckman Coulter Genomics) and 383 µl 2.5M Sodium Chloride ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with following antibody mix for 30 minutes on ice: pan-γδTCR-PE (1:10; IMMU510; Beckman Coultier) and CD69-APC (1:25 ...
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bioRxiv - Cancer Biology 2024Quote: ... The fragments were repaired the 3’and 5’overhangs using End repair mix (component of SureselectXT) and purified using Agencourt AMPure XP Beads (Beckman). The purified fragments were added with ‘A’ tail using A tailing Mix (component of SureSelectXT ...
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bioRxiv - Microbiology 2021Quote: ... triplicate spore preparations were made in 3 ml of 20% glycerol and suspended in Isoton II (Beckman Coulter, Brea, CA, USA). The particle volume was then measured for a minimum of 100 000 particles per sample using a Multisizer 4e Coulter counter (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... Washed pellets were resuspended in 2 mL 1x PBS and fluorescence was calculated using a LSR II cell analyzer (Beckman Coulter). Two biological replicates of 50,000 cell counts were analyzed with FlowJo ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted to a DNA concentration of 50 ng/µl followed by ligation of 454 sequencing adaptors and molecular identifier (MID) tags using the SPRIworks Fragment Library System II for Roche GS FLX+ DNA Sequencer (Beckman Coulter), excluding fragments smaller than 350 base pairs ...
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bioRxiv - Developmental Biology 2022Quote: ... The nuclei suspension in the HB + GM mix was layered over the 29% cushion and centrifuged in an SW41Ti rotor (Beckman Coulter) at 7700 rpm and 4°C for 30 min ...
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Fetal estrogens are not involved in sex determination but critical for early ovarian differentiationbioRxiv - Developmental Biology 2021Quote: ... Anti Müllerian hormone levels were determined in 50 μl aliquots of serum samples by using an ELISA kit (AMH GenII ELISA, with AMH Gen II calibrators and controls, Beckman Coulter, Villepinte, France) as previously described (Bourdon et al ...
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bioRxiv - Molecular Biology 2020Quote: ... entire ChIPed DNA or 300ng of the input DNA were used to construct libraries using NEBNext® Ultra™ II DNA Library Prep kit with AMPure XP magnetic beads (Beckman Coulter). Library quality and quantity were estimated with Bioanalyzer and Qubit assays ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We prepared sequencing libraries using the New England Biolabs Ultra II kit with the only modification being the use of Ampure XP beads for cleanup and size selection (Beckman Coulter, Brea, CA, USA). Following adapter ligation a single size selection was conducted at 0.65X ...
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bioRxiv - Microbiology 2023Quote: Stm-infected HeLa cells were FACS-sorted in DsRed-dim and DsRed populations using the CytoFLEX SRT II cell sorter (Beckman Coulter Fullerton, CA, USA) of the Flow Cytometry Core Facility of the Leiden University Medical Centre to determine the intracellular bacterial burden of both populations ...
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bioRxiv - Cell Biology 2019Quote: ... instrument light scatter calibration was performed by fitting acquired 405 nm light scatter data to predicted values using the reported diameters and refractive indices of Apogee Mix beads (Apogee info) and NIST-traceable beads (Beckman Coulter, Brea, CA). These ranged in diameter from 80 nm polystyrene to 1300 nm silica (Fig ...
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bioRxiv - Cell Biology 2019Quote: ... The mix was then airfuged 5 min at 30 psi with an Airfugee® Air-driven ultracentrifuge (Beckman Coulter Inc., Brea, California, USA) and the pellet was re-suspended in 80% of the initial volume and left on ice for 20 min to depolymerize the microtubules ...
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bioRxiv - Cell Biology 2023Quote: ... the resulting G-actin mix was ultracentrifuged at 90K rpm for 30 minutes at 4°C in an ultracentrifuge (Beckman, Optima TL 100) to remove actin oligomers and/or aggregates.
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bioRxiv - Genetics 2021Quote: ... The DNA was end-prepped with the NEBNext FFPE Repair and Ultra II End Prep Kit and purified using Agencourt AMPure XP (Beckman Coulter Inc., Brea, CA, USA). Then the adapter ligation was performed using NEBNext Quick T4 DNA Ligase ...