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Citations for Beckman :
51 - 100 of 106 citations for Frizzled 8 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Libraries were amplified for a total of 8-10 cycles and DNA purified using AMPure XP beads (Beckman Coulter). The quality and concentration of ATAC-seq libraries were evaluated using the Bioanalyzer DNA High Sensitivity Assay (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were collected by centrifugation (8 000 rpm for 10 min at 4 °C, JLA-16.25 Beckman Coulter rotor), pellets frozen in liquid nitrogen and stored at −80°C ...
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bioRxiv - Systems Biology 2021Quote: ... we pooled all 8 reactions for each sample and purified the PCR product using AMPure XP beads (Beckman Coulter). The second PCR was used to link specific indexes to each sample so we can multiplex several samples in a single sequencing run.
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bioRxiv - Genomics 2019Quote: Human RNA libraries were individually cleaned using Agencourt AMPure XP beads (Beckman Coulter, #A63881) and a double-sided procedure (left 1×-right 0.6×) ...
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bioRxiv - Immunology 2022Quote: ... Human Vγ9Vδ2 T lymphocytes were phenotyped using PE-labeled anti-NKG2A (#Z199, Beckman Coulter), PE-labeled anti-NKG2D (#ON72 ...
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bioRxiv - Microbiology 2022Quote: ... human PBMC were incubated with monoclonal antibodies against CD19 (PE-Cy7; Beckman Coulter; IM3628), CD3 (PerCP ...
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bioRxiv - Biochemistry 2022Quote: ... To that was dispensed TMPRSS2 (reconstituted in 50% glycerol at 8.75 μM, 22x) in assay buffer (50 mM Tris pH 8, 150 mM NaCl) using a BioRAPTR (Beckman Coulter) to give a total reaction volume of 50 μL ...
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bioRxiv - Biochemistry 2022Quote: ... To that was dispensed TMPRSS2 (reconstituted in 50% glycerol at 8.75 μM, 50x) in assay buffer (50 mM Tris pH 8, 150 mM NaCl, 0.01% Tween20) using a BioRAPTR (Beckman Coulter) to give a total reaction volume of 5 μL ...
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bioRxiv - Genomics 2021Quote: ... The combined 48 µl pools in the 8-well strip were incubated with 58 µL AMPure XP beads (Beckman Coulter) (1.2x bead to sample ratio ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was transferred to an ultracentrifuge tube and overlayed with layers of Lysis Buffer A with 12% Ficoll and Buffer B with 8% Ficoll (AAB2209518, Fisher) and centrifuged at 28,500 rpm for 45 min (Beckman 42.1). Afterwards ...
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bioRxiv - Neuroscience 2020Quote: ... Groups of 8 reactions were pooled to yield ∼250 μL and purified with Ampure XP Beads (0.6x ratio; Beckman Coulter), washed twice with 75% Ethanol ...
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bioRxiv - Microbiology 2021Quote: ... 60% w/v) and centrifuged at 64,000 x g for 3 hours in A27-8×50 mL rotor (Beckman Coulter). The visible virus bands were collected ...
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bioRxiv - Genetics 2023Quote: ... Libraries were pooled up to 95-plex in equimolar amounts on a Biomek NX-8 liquid handling platform (Beckman Coulter), quantified using a High Sensitivity DNA Kit on a 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were sheared with a 25-gauge 5/8 needle and loaded onto CsCl solution (150% w/v) for ultracentrifugation in NVT 65.2 rotor (Beckman coulter) at 42,000 rpm for 20 hours at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were sonicated for 6-8 cycles of 20 secs and centrifuged at 40.000 rpm in MLA 80 rotor (Beckman coulter) for 45 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Populations were transferred twice a week by diluting 15% of the culture into 800 µL of fresh medium using a liquid-handling robot (Biomek NXP Span-8; Beckman Coulter). At each transfer ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was prepared using Takara Clontech SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Cat.No.:634890) using 8 cycles of amplification and purified using Agencourt AMPure XP beads (Beckman Coulter, A63880). 1 ng of each sample cDNA was used for library preparation ...
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bioRxiv - Biochemistry 2020Quote: ... To that was dispensed TMPRSS2 (150 nL) in assay buffer (50 mM Tris pH 8, 150 mM NaCl) using a BioRAPTR (Beckman Coulter) to give total reaction volume of 5 µL ...
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bioRxiv - Systems Biology 2020Quote: We used two independent robot platforms with a similar basic configuration of pipettor systems (Biomek NXP automated workstations with Multichannel-96 and Span-8 pipetting model, Beckman Coulter). Additional instruments were integrated with the original workstations including incubators (Incubator Shaker DWP ...
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bioRxiv - Neuroscience 2020Quote: ... samples with different indexes but of the same source (somata, dendrites or empty cuts) were pooled together in groups of 8 and purified using AMPure XP beads (Beckman Coulter). Libraries were further indexed ...
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bioRxiv - Genomics 2022Quote: ... 1 µg of whole-genome amplified gDNA was sheared into ∼8 kb fragments followed by purification and size-selection using AMPure XP beads (Beckman Coulter). Oxford Nanopore Technology (ONT ...
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bioRxiv - Genomics 2022Quote: ... The non-WGA sample was used to prepare an ONT library by shearing the DNA into ~8 kb fragments followed by purification and size-selection using AMPure XP beads (Beckman Coulter). The ONT library was then prepared with the Ligation Sequencing Kit (SQK-LSK109 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was split into 8 fractions and exosomes were pelleted in two runs of ultracentrifugation (Optima LE-80K, Beckman Coulter) at 125,000 g for 45 min at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... unstimulated PBMC cells (BenTech, Seattle, WA) were pelleted by centrifugation 228 x g for 8 min in an Allegra centrifuge (Beckman, Brea, CA). Pellets were resuspended in activation medium containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech ...
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bioRxiv - Biochemistry 2020Quote: ... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
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bioRxiv - Neuroscience 2021Quote: ... 100 mL of each well in the 8-well strip was purified with 60 mL Ampure XP beads (0.6x ratio; Beckman Coulter, Brea, CA), washed twice with 75% ethanol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Mitochondria were purified using a series of differential centrifugations: 20 min at 1500 g to pellet residual chloroplasts (8 °C, 3500 RPM, Beckman JA-12), 20 min at 20 000 g to pellet mitochondria (8 °C ...
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bioRxiv - Immunology 2021Quote: ... was loaded onto the top of 6–8 M GuHCl gradients and centrifuged in a Beckman Optima L-90K Ultracentrifuge (Beckman SW40 rotor) at 40,000 rpm for 2.75 h 15°C ...
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bioRxiv - Cell Biology 2021Quote: ... platelets in PRP were labeled with a mouse anti-human monoclonal antibody CD61-PE (Beckman Coulter). Platelet activation was detected by direct immunofluorescence labeling of alpha granule release marker CD62P (P-selectin ...
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bioRxiv - Cell Biology 2021Quote: Platelets in PRP were labeled with a mouse anti-human monoclonal antibody CD61-PE (Beckman Coulter). Incubation was performed at room temperature for 10 minutes in the dark ...
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bioRxiv - Microbiology 2021Quote: ... and 10μl of PE-conjugated mouse-anti-human langerin Ab (Clone DCGM4; Beckman Coulter, Marseille, France). In other experiments ...
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bioRxiv - Microbiology 2019Quote: ... A portion of each sample was stained with Mouse-Anti Human CD15-PE (Beckman Coulter, USA) and run under BD Accuri C6 Flow Cytometer ...
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bioRxiv - Cell Biology 2020Quote: ... Samples are then resuspended in PBS and incubated with APC-A700 anti-human CD56 (N901, #B92446 Beckman Coulter), APC-A750 anti-human CD90 (Thy-1/310 ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentration of the expanding human T cells was calculated on a Coulter Multisizer (Beckman Coulter, Brea, CA) and maintained at 1.0-2.0 × 106 cells per mL in R10 media (RPMI-1640 plus GlutaMAX-1 ...
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bioRxiv - Immunology 2022Quote: ... GEM-RT products were subsequently amplified using human BCR primers and cleaned up through SPRIselect beads (Beckman Coulter). Next ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of Caco-2 cells and human ileum organoids was extracted using RNAdvance Tissue kit (Beckman Coulter) and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research) ...
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bioRxiv - Bioengineering 2023Quote: ... or by staining with anti-human TCR mAbs (Vβ13.1-IMMU 222, Beckman Coulter for A2/NY A97L-TCR). Cell staining was performed at 4°C for 30min.
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bioRxiv - Evolutionary Biology 2022Quote: ... and serial dilutions were performed for each antibiotic using an automated culture system [34] consisting of a Biomek®NX span-8 laboratory automation workstation (Beckman Coulter, Brea, California, USA) in a clean booth connected to a microplate reader (FilterMax F5 ...
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bioRxiv - Immunology 2022Quote: ... human Vγ9Vδ2 T lymphocytes were collected and stained with a FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and analyzed by flow cytometry ...
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bioRxiv - Cancer Biology 2024Quote: ... Platelet concentration was obtained by flow cytometry using an antibody against human CD41 labelled with PC7 (6607115, Beckman Coulter) and MP-count beads (6607115 ...
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bioRxiv - Immunology 2024Quote: Cells were washed in DPBS plus 0.5% human serum albumin and incubated with anti-CD45-KrO (#B36294, Beckman Coulter), anti-CD3-APC (#IM2467 ...
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bioRxiv - Microbiology 2021Quote: ... the percentages of anti-human IgG postive cells in GFP positive cells were then analyzed using flow cytometer (Cytoflex S, Beckman). LIVE/DEAD fixable aqua (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... PCR products with no hashtag libraries (spleen, mouse lung, and human lung cells) were combined and purified twice with 0.65× AmPure XP beads (Beckman Coulter) and eluted with 21 μL of nuclease-free water ...
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bioRxiv - Cancer Biology 2020Quote: ... The virally-transduced human CD34+ were sorted for GFP reporter expresssion on a MoFlo high-speed cell sorter (Beckman-Coulter) located at the New York Blood Center ...
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bioRxiv - Immunology 2021Quote: ... as assessed by flow cytometry with Korman orange-conjugated mouse anti-human CD19 monoclonal antibody (mAb) (Beckman Coulter, CA, USA). The purified CD19+ B cells were cultured in the chemical-defined and serum-free X’VIVO 15 medium (Lonza ...
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bioRxiv - Immunology 2023Quote: Human TCR Vβ repertoire analysis was performed using antibodies against the most frequent TCR Vβ variants (covering about 70% normal human TCR Vβ repertoire, as reported by Beckman Coulter “Beta Mark TCR Vbeta Repertoire Kit” (cat.nr. ...
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bioRxiv - Cell Biology 2019Quote: Viability of unlabeled and ferumoxytol-labeled murine MSCs (n=4) and human MSCs (n=3) was assessed by Trypan blue dye exclusion using ViCell (Beckman Coulter).
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bioRxiv - Cancer Biology 2019Quote: Cells were stained for 30 min (minutes) with the anti-human monoclonal antibody CD11b-PE (IM2581U, Beckman Coulter, Brea, CA, USA), CD14-FITC (555397 ...
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bioRxiv - Cancer Biology 2024Quote: Analysis of GALNT3 or fibronectin expression on human or mouse pancreatic cancer cells was performed using a flow cytometer (Gallios, Beckman Coulter) with appropriate antibodies ...
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bioRxiv - Immunology 2024Quote: ... The sections were washed and incubated for two hours with anti-human mouse CD14 Texas Red (clone: RMO52) (Beckman Coulter, USA). Afterward ...