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Citations for Beckman :
1 - 50 of 180 citations for Eukaryotic Peptide Chain Release Factor GTP Binding Subunit ERF3A GSPT1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 0.1 mM GTP) layered in TLS-55 (Beckman) centrifuge tubes ...
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bioRxiv - Immunology 2020Quote: ... Antibody binding was analyzed by flow cytometry (FACS CytoFLEX; Beckman Coulter®) by determination of the mean fluorescence intensity (MFI ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM GTP in a TLA110 rotor (Beckman, Palo Alto, CA) at 80,000 rpm ...
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bioRxiv - Genomics 2024Quote: ... cDNA release and collection is performed by incubating the chip in cDNA release mix overnight at 55*C and the release cDNA is purified with Ampure XP beads (Beckman Coulter; Cat. No. A63882) using the manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with GTP) was thawed and spun in an ultracentrifuge (Beckman Coulter OptimaMax) at 70k rpm for 5 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Ribosomal subunits were pelleted using a TLA100.3 rotor (Beckman Coulter, 349490) at ∼300,000 xg at 4 °C for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... k factor 81.3 (Beckman Coulter). RNA was isolated from the pelleted samples with a miRNeasy micro kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... Centrifugations at 10,000 g (k-factor = 2547.2) and 100,000 g (k-factor = 254.7) were done at +4°C using polyallomer tubes (Beckman Coulter Inc. ...
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bioRxiv - Biophysics 2024Quote: ... The subunits were then separated by ultracentrifugation in an SW32 rotor (Beckman Coulter, Catalog Number 369694) at 22,000 rpm for 17 hours at 4 °C.
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bioRxiv - Bioengineering 2024Quote: ... the stained cells were washed twice to remove any unspecific binding antibodies and were then subjected to measurement using a Beckman Cytoflex S flow cytometer (Beckman, CA, USA). The resulting data were analyzed using FlowJo software (Tree Star ...
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bioRxiv - Immunology 2022Quote: ... Fibronectin binding was assessed by flow cytometry (CytoFlex, Beckman Coulter). GFP-expressing bacteria were assessed by gating for FITC positive events ...
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bioRxiv - Microbiology 2024Quote: ... Binding was analyzed by flow cytometry (Cytoflex S, Beckman Coulter) by comparing the TAMRA-signals (PE-channel ...
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bioRxiv - Molecular Biology 2020Quote: Binding to CD16 was studied by a competitive assay with an anti-CD16-PE/3G8 antibody conjugated to phycoerythrin (Beckman Coulter, Brea, CA, USA) and unlabelled test and control antibodies using Jurkat-CD16 cells.
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bioRxiv - Microbiology 2023Quote: ... The AMPure XP binding beads (Beckman Coulter Genomic, Indianapolis, IN, USA).
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bioRxiv - Biophysics 2022Quote: ... the 30S and 50S subunits were separated on a 15-35% (w/v) sucrose gradient in dissociation buffer on a SW-32 rotor (Beckman-Coulter) spun at 28,000 rpm (97,000 xg ...
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bioRxiv - Cell Biology 2020Quote: ... EVs were ultracentrifuged (Type 45 Ti rotor, k-Factor 133, Beckman Coulter) with 100000 g for 90 min 4°C ...
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bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Subunit Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Subunit Buffer in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
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bioRxiv - Bioengineering 2023Quote: ... cells were analyzed for target binding using a CytoFLEX flow cytometer (Beckman Coulter). Background-subtracted and normalized binding curves were fitted to a first- order binding model and equilibrium dissociation constant (KD ...
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bioRxiv - Microbiology 2020Quote: ... DNA sequences were determined by the dideoxy chain termination method (Beckman Coulter Genomic Center at Denver, MA). The uidA and mutS sequences were submitted to Genbank (http://www.ncbi.nlm.nih.gov/genbank/ ...
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bioRxiv - Cell Biology 2021Quote: ... by binding samples with a 1.5 X volume of AMPure XP beads (Beckman Coulter), washing twice with 70 % ethanol ...
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bioRxiv - Microbiology 2021Quote: ... the samples were resuspended in binding buffer and analysed using a cytofluorimeter (CytoflexS, Beckman) with excitation at 488 nm and 525 nm emission for Annexin and 585 nm emission for PI ...
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bioRxiv - Biochemistry 2024Quote: ... Annexin V and PI binding were analyzed using an CytoFLEX flow cytometer (Beckman Coulter), and the percentage of apoptotic cells was calculated from the total number of cells (∼1 × 104 cells ...
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bioRxiv - Immunology 2023Quote: To purify the BCR heavy chain amplicons (∼600 bp) a 0.5–0.8x double-sided bead clean-up was performed (Beckman Coulter) before quantification by Qubit (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... fixed angle Type 45 Ti rotor (k factor = 133; Beckman Coulter, Miami, FL, USA), at 4°C ...
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bioRxiv - Immunology 2022Quote: ... The PCR products of the heavy and light chain variable regions were purified with SPRI beads according to the manufacturer’s instructions (Beckman Coulter). Then ...
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bioRxiv - Genetics 2021Quote: ... The amplified libraries were pooled and purified again by binding to AMPure XP (Beckman-Coulter #A63880) beads (0.9 beads:1 sample) ...
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bioRxiv - Microbiology 2023Quote: ... the PCR products were cleaned using AMPure XP binding beads (Beckman Coulter Genomic, Indianapolis, IN, USA). The AMPure XP binding beads (Beckman Coulter Genomic ...
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bioRxiv - Neuroscience 2020Quote: ... we generated double-stranded DNA template for transcription of each sgRNA via a template-free polymerase chain reaction (PCR) with partially overlapping primers (IDT, PAGE purified) and purified the product with Ampure XP beads (Beckman Coulter, A63880). We then transcribed sgRNAs in vitro using the MEGAscript T7 Transcription Kit (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... 200 μL of 1x binding buffer were added and suspension was analyzed by flow cytometry (Beckman-Coulter, CytoFLEX). Flow cytometry gates were chosen based on untreated controls for each cell line and values were normalized to controls.
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bioRxiv - Cancer Biology 2024Quote: ... and the FOXP3/Transcription Factor Staining Kit (eBioscience) and run on the CytoFlex S cytometer (Beckman Coulter). Analysis was performed in FlowJo.
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was centrifuged at 10,500 rpm (∼20,000 × g, k factor = 1,753) for 70 min (SW28 rotor, Beckman). Pelleted lEVs were resuspended in 13.5 ml of PBS (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... and sedimented again at 10,500 rpm (∼20,000 × g, k factor = 1,983) for 70 min (SW40 rotor, Beckman). The supernatant containing sEVs was centrifuged at 25,000 rpm (∼ 110,000 × g ...
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bioRxiv - Biochemistry 2021Quote: ... The library was cleaned up and size-selected by two consecutive rounds of binding to 1.2x and 0.8x SPRI beads (Agentcourt AMPure XP beads, Beckman Coulter). The integrity and size distribution of the final libraries (two biological replicates of KCl-treated and one of untreated ...
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bioRxiv - Genomics 2021Quote: ... Estradiol (E2) and Progesterone assays are competitive binding immunoenzymatic assays performed on the Unicel DXI 800 system (Beckman Coulter). FSH assay is based on Microparticle Enzyme Immunoassay (MEIA ...
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bioRxiv - Microbiology 2022Quote: ... 400 μL of 1X binding buffer was then added before analysing using a CytoFLEX S flow cytometer (Beckman Coutler). Annexin V-FITC positive cells were designated as early apoptotic ...
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bioRxiv - Biochemistry 2023Quote: ... binding reactions were centrifuged at 50,000 RPM for 30 min at 25 °C in a TLA-100 rotor (Beckman). Following centrifugation ...
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bioRxiv - Biochemistry 2023Quote: ... binding reactions were centrifuged at 50,000 RPM for 30 min at 25 °C in a TLA-100 rotor (Beckman). Promptly after centrifugation ...
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bioRxiv - Cell Biology 2021Quote: ... 100K EVs were isolated by ultracentrifugation of 10,000xg supernatant at 100,000xg for 65 min in a SW40 rotor (k-factor 280.3) (Beckman-Coulter). EV-containing 100K ultracentrifugation pellets were resuspended in 20 μL PBS containing 0.2% BSA (cleared from aggregates by ultracentrifugation for 16-20 hrs at 100,000xg) ...
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bioRxiv - Plant Biology 2021Quote: ... Incorporated P33 in AMARA peptide was quantified using a liquid scintillation counter (Beckman, CA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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bioRxiv - Genomics 2023Quote: Sheared DNA was purified by binding to an equal volume of AMPure XP beads (A63881, Beckman Coulter, Inc. Brea, CA) and eluted in 50µl of 10mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and the pellet was resuspended in 25 ∝l lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and Red-C12 fluorescence of the starting material ...
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bioRxiv - Physiology 2024Quote: ... inter-assay CV 6.4-19.2%) were measured via a competitive binding immune-enzymatic assay according to manufacturer’s instructions (Beckman Coulter, Lane Cove, Australia). Luteinising hormone (LH ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed once in FACS buffer and ligand binding was measured by flow cytometry on a Cytoflex instrument (Beckman Coulter). 104 events were collected per sample ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed once in FACS buffer and mAb binding was measured by flow cytometry on a Cytoflex instrument (Beckman Coulter). 104 events were collected per sample ...
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bioRxiv - Molecular Biology 2020Quote: ... columns were spun at 100.000 g for 24 hours using a SW-28.1 rotor (k-factor: 276) in an optima XPN-80 ultracentrifuge (Beckman coulter), and subsequently fractions of 1 mL were collected ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 300,000×g at 4°C for 3 h in a TLA-110 fixed angle rotor (k-factor 13) (Beckman Coulter). The pellet was resuspended in 10 µL PBS of which 3 µL was adsorbed onto glow-discharged 2 nm carbon layer coated Quantifoil R1.2/1.3 400 mesh gold grids ...
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bioRxiv - Systems Biology 2023Quote: ... The urine was centrifuged at 20,000 x g for 30 minutes at 4°C (k-factor 1,790) in an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW32 Ti swinging bucket rotor (Rmin 67 ...
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bioRxiv - Biochemistry 2021Quote: ... Binding reactions were spun at 300,000 g for 30 min at 25 °C using Optima MAX-TL ultracentrifuge (Beckman Coulter, Brea, CA). Bundling reactions were spun at 17,000 g for 15 min at 25 °C ...