Labshake search
Citations for Beckman :
201 - 250 of 1240 citations for Ephrin type A receptor 4 EphA4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... After increasing the NH4Cl concentration of the supernatant to 1 M to dissociate bS1 from 70S ribosomes the sample was centrifuged in a Type 70 Ti rotor (Beckman) at 60,000 g for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Ultracentrifugation was performed using a SW Type 32.1 Ti rotor in Optima XPN-100 Ultracentrifuge (Beckman Coulter, Brea, CA, USA) at 186,000g for 18 h at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... by dounce homogenization (20 strokes) and the homogenate was centrifuged at 55,000 rpm in a Type 70 Ti rotor (Beckman Coulter) for 1 hr ...
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bioRxiv - Neuroscience 2021Quote: ... The EV pellet was resuspended in PBS and centrifuged at 40,000 rpm for 70 min using the type 70 Ti rotor in an Optima XPN-80 ultracentrifuge (Beckman Coulter). The pellet obtained after this last centrifugation step was the purified EV fraction ...
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bioRxiv - Neuroscience 2021Quote: ... 40% and 60% iodixanol gradient and was spun at 184,000 x g (50,000 rpm in a Beckman Type 70Ti rotor) for 3 hours 20 minutes at 10°C ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant from this step was then subjected to ultracentrifugation at 200,000g for 35 min (Beckman Type 45 Ti rotor) to collect microsomes ...
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bioRxiv - Microbiology 2020Quote: ... Whole-cell lysates were then spun at 35,000 rpm for 45 min in a Type 45 Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Bioengineering 2022Quote: ... the conditioned media was subjected to an additional centrifugation step of 118,000 × g for 2 h in a Type 70 Ti ultracentrifuge rotor (Beckman Coulter). The resulting EV pellet was resuspended in 1X PBS and placed into a Nanosep 300 kDa MWCO spin column (Pall ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were cleared by centrifugation at 50,000 × g for 40 min in a Type 45 Ti fixed-angle rotor (Beckman Coulter). Pre-equilibrated Pierce High Capacity Streptavidin agarose resin (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were cleared by centrifugation at 50,000 × g for 40 min in a Type 45 Ti fixed-angle rotor (Beckman Coulter). Preequilibrated Ni2+-nitrilotriacetic acid (NTA ...
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bioRxiv - Microbiology 2022Quote: ... The sample-containing tube was spun at 347,000 × g and 20°C for 3 hours using Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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bioRxiv - Biochemistry 2023Quote: Sedimentation of high molecular weight species before aggregation was carried out using a Type 50.2 Ti fixed-angle rotor in an Optima Ultracentrifuge (Beckman Coulter). Samples were centrifuged at 40,000 rpm (average 146,000 g) ...
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bioRxiv - Neuroscience 2023Quote: ... the crude lysate was subjected to iodixanol gradient (15%, 25%, 40% and 60%) ultracentrifugation using a Type 70Ti rotor (Beckman) at 490000 × g ...
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bioRxiv - Biochemistry 2023Quote: ... by dounce homogenization (20 strokes).The lysate was clarified by centrifugation at 56,000 rpm in a Type 70 Ti rotor (Beckman Coulter) at 4°C for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were ultracentrifuged at 4 °C and 150,000 ˗ g for 2 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) and supernatants were removed ...
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bioRxiv - Bioengineering 2024Quote: ... The sample tubes were then centrifuged at 347,000 × g and 20°C for 3 hours using a Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C in a TLA 100.3 rotor (Beckman). P13 ...
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bioRxiv - Biochemistry 2020Quote: ... 4 °C (Beckman Coulter Optima LE-80K Ultracentrifuge). The clarified lysate was placed in a beaker on a stir plate at 4 °C and concentrated ammonium sulfate (5 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2019Quote: ... 4°C (Optima™ MAX Ultracentrifuge, Beckman Coulter) to sediment amorphous aggregates ...
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bioRxiv - Neuroscience 2020Quote: ... for 4 h with SW28 rotor (Beckman Coulter). The concentrated vector was stored at −80 °C until use ...
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bioRxiv - Biochemistry 2022Quote: ... 4 °C on a Sorvall WX90 ultracentrifuge (Beckman) with SW55Ti rotor ...
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bioRxiv - Molecular Biology 2022Quote: ... 4°C in SW-41Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Plant Biology 2021Quote: ... 4 °C for 18 h (Beckman SW41 rotor). The sugar solution contains 0.65 M sucrose ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C using a SW41Ti rotor (Beckman Coulter). A BioComp fractionator was used to collect fractions 1 – 16 ...
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bioRxiv - Neuroscience 2022Quote: ... at 4°C in a Beckman ultracentrifuge (Beckman). After centrifugation ...
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bioRxiv - Systems Biology 2022Quote: A particle size analyzer (Multisizer 4, Beckman Coulter) was used to count the number of cells ...
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bioRxiv - Immunology 2021Quote: ... including PE-Cy7-conjugated mouse anti-human CD45 (Beckman Coulter, CA, USA), efluor660-conjugated rat anti-human OCT3/4 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... while human B cells were CD38+/- (Beckman Coulter #6699531; 1µl per test) and CD19 (BD #557791 ...
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bioRxiv - Neuroscience 2021Quote: ... 4 × 5-s bursts) and subsequently centrifuged at 48,000g at 4°C (Beckman Avanti J-251 Ultracentrifuge; Beckman Coulter, Fullerton, CA) for 30 min ...
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bioRxiv - Immunology 2021Quote: ... were incubated with monoclonal antibodies at 4°C for 30 min and then fixed with 4% paraformaldehyde before analysis on a Cytoflex cytometer (Beckman Coulter). Data were analyzed by Flow Jo software v.10.7 (BD Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: ... and loaded onto of a step-wise sucrose gradient as described by the manufacturer and subjected to ultracentrifugation at 150,000 xg for 4 h at 4 °C using SW50.1 rotor (Beckman Coulter, Mississauga, ON). Fractions were then collected and subject to denaturation with Laemmli buffer until further use.
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was filtered with a membrane pore size of 0.2 μm (Whatman) and centrifuged at 120,000 × g for 4 h at 4 °C in polycarbonate bottle assemblies (Beckman coulter, 355603) in a 70.1 Ti fixed angle rotor (Beckman coulter ...
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bioRxiv - Plant Biology 2022Quote: Protoplasts from wild type pCOBL9:GFP and cas-c1 pCOBL9:GFP transgenic plants were selected in a sorter MoFlo Astrios EQ (Beckman-Coulter), with a 70 μm nozzle at a rate of 5,000 to 10,000 events per second and a fluid pressure of 60 psi ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were filled into 38 mL open-top polypropylene tubes and subjected to DUC using a SW Type 32 Ti swinging bucket rotor (Beckman Coulter) in an Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Neuroscience 2021Quote: ... The cleared supernatant was subsequently centrifuged at 40,000 rpm for 70 min to pellet the EVs using the type 70 Ti rotor in an Optima XPN-80 ultracentrifuge (Beckman Coulter). The supernatant resulting from this high-speed centrifugation is the EV-free ACM ...
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bioRxiv - Immunology 2020Quote: ... and the boundary layers between the two sucrose layers were harvested after ultracentrifugation (90 min, 51,000 rpm, 18 °C, Optima™ L90K centrifuge, rotor type: 70.1 Ti, tubes: 16 * 76 mm, Beckman Coulter, USA). These fractions were subsequently mixed with 6 M CsCl (AppliChem ...
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bioRxiv - Microbiology 2023Quote: ... Archaella were precipitated from the supernatant at 142400 g using Beckman Coulter Opima XE-90 ultacentrifuge and the fixed-angle titanium rotor Type 45 Ti (Beckman Coulter). The tafi remained in the supernatant and could be precipitated either during many hours of high-speed centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... viruses were collected and concentrated by ultracentrifugation (37,000 rpm, 2 h 30, 15 °C; Beckman Type 45 Ti fixed-angle rotor). The virus titer was determined using a plaque assay.
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bioRxiv - Cell Biology 2023Quote: ... The UC step was an EV depletion step where the FBS was ultracentrifuged at 118,000 × g (Type 45 Ti rotor, 38,800 rpm, Beckman Coulter, Brea, CA) for 18 h at 4°C followed by a filtration step (0.22 μm) ...
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bioRxiv - Molecular Biology 2024Quote: Density gradient ultracentrifugation using a SW Type 55 Ti swinging bucket rotor in LM-70 ultracentrifuge (Beckman Coulter, Brea, CA, USA) was achieved using OptiPrepTM iodixanol solution (60% iodixanol ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was separated and centrifuged for 2.5 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge and a Type 45 Ti rotor (Beckman Coulter, USA). The supernatant was discarded ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was separated and centrifuged for 3 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge with a Type 45 Ti rotor (Beckman Coulter, USA). The supernatant was discarded ...
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bioRxiv - Immunology 2024Quote: ... The gradient section containing assembled encapsulins was removed via aspiration and nanocontainers were isolated in a final ultracentrifugation step in a Type 70Ti rotor (Beckman Coulter) spinning at 68,000 rpm for 2 hours at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... 0.2% Triton X-100 and 100 µg/mL chloramphenicol) and ultracentrifuged at 42500 rpm for 5 h in type 70 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... and centrifuged at 30,000 rpm for 4 h at 4°C using an SW-40 rotor in the Optima L-90K Ultracentrifuge (Beckman Coulter, USA). To identify resultant density gradient fractions containing virus particles ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was filtered by 0.22 μm nylon membrane followed by centrifugation through a cushion of 50 % (w/v) sucrose with 36,000 rpm at 4 °C for 4 h (Beckman SW41 Ti rotor). The pellet was resuspended with PBS and the copy number of DWV was quantified by qRT-PCR.
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bioRxiv - Biophysics 2024Quote: ... The gradients were then centrifuged at 187,000 × g for 4 h at 4°C in a SW 41 Ti rotor (Beckman Coulter, Brea, CA). Bands corresponding to purified virus particles (∼1.36 g/mL ...
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bioRxiv - Microbiology 2020Quote: ... 4°C in SW 60 Ti rotor (Beckman Coulter) to pellet cyanophage particles ...
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bioRxiv - Immunology 2021Quote: ... CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1 ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C (SW32Ti rotor, Optima L-80XP ultracentrifuge, Beckman) with the maximum acceleration and minimum deceleration ...