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Citations for Beckman :
501 - 550 of 2555 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... The cell viability and the size were calculated using a Vi-CELL XR Cell Viability Analyzer (Beckman Coulter). The cells were finally washed and resuspended in PBS/MgCl2/CaCl2 (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... Additional aliquots were analyzed for viability and cell number using a Vi-Cell XR Cell Counter (Beckman Coulter) and all samples were determined to have >98% viability based on trypan blue exclusion ...
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bioRxiv - Immunology 2021Quote: ... Cell counts and viability were determined using a Vi-CELL XR Automated Cell Counter (Beckman Coulter Life Sciences). Equal number of cells were aliquoted from each sample to create a pool of 1.5×106 cells with an average viability of 85% or higher ...
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bioRxiv - Cancer Biology 2024Quote: ... cell count and viability were measured using the Vi-Cell™ XR Cell Viability Analyzer (Beckman Coulter, USA). The cells were seeded as single cell suspension in non-adherent culture flasks at a density of 1.2 x104 cells/cm2 ...
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bioRxiv - Developmental Biology 2021Quote: ... PαS cells were sorted using MoFlo XDP Cell Sorter (Beckman Coulter) or FACSAria III (BD ...
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bioRxiv - Cancer Biology 2019Quote: ... an aliquot of cells was counted (Vi-CELL XR, Beckman Coulter), and cells were diluted in fresh media to maintain them in logarithmic growth phase ...
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bioRxiv - Cancer Biology 2020Quote: ... EGFP+ cells were sorted by MoFlo Astrios cell sorter (Beckman Coulter) and subjected to microarray analysis.
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bioRxiv - Bioengineering 2021Quote: ... cell debris and other cell secretions (Beckman Coulter, Allegra X-30R). The supernatants were then centrifuged at 100,000 × g for 90 min (Beckman Coulter ...
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bioRxiv - Plant Biology 2020Quote: ... Cell growth was followed using a cell counter Multisizer (Beckman Coulter). For day night cycle experiment ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were counted with Vi-Cell™ XR (Beckman Coulter, IN) using Trypan Blue for cell viability assessment and 30 million cells were pelleted per replicate for T0 time points ...
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bioRxiv - Cancer Biology 2020Quote: ... were counted using a Vi-Cell XR cell counter (Beckman Coulter). Cells were then pelleted by centrifugation flash frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Immunology 2020Quote: ... Live single cells were sorted on MoFloXDP Cell Sorter (Beckman Coulter), using the services of the Hospital for Sick Children Flow Cytometry Facility ...
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bioRxiv - Genetics 2021Quote: ... used for single cell dispersion and Vi-Cell XR (Beckman Coulter) for counting ...
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bioRxiv - Developmental Biology 2022Quote: ... and cells were counted by an automated Vi-Cell (Beckman Coulter). All incubations were carried out at 37°C ...
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bioRxiv - Cell Biology 2023Quote: Cells were sorted on a MoFlo Astrios Cell Sorter (Beckman Coulter) running Summit software ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently counted with a Vi-CELL analyzer (Beckman Coulter), and 3-5×106 cells were used per antibody staining reaction.
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bioRxiv - Immunology 2023Quote: ... Cells were then counted with a Vi-CELL analyzer (Beckman Coulter) and plated at a density of 8×106 cells per well in 6-well plates ...
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bioRxiv - Biochemistry 2023Quote: ... and counted using Vi-cell XR cell viability analyzer (Beckman Coulter). The cells were then diluted with cell culture media to a concentration of ca ...
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bioRxiv - Cell Biology 2023Quote: ... cells were sorted by a cell sorter (MoFlo AstriosEQ; Beckman Coulter)
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bioRxiv - Cancer Biology 2021Quote: ... The cell number and viability were determined on a Vi-Cell XR cell viability analyzer (Beckman Coulter, CA, USA) and scRNAseq library preparation was performed using dual single cell mouse kit 5’/TCR according to the manufacturer’s instructions (10x Genomics ...
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bioRxiv - Cell Biology 2020Quote: ... ‘Reserve’ cells were sorted by gating a mononuclear GFP+ cell population using the MoFlo XDP cell sorter (Beckman Coulter).
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bioRxiv - Cancer Biology 2022Quote: ... GFP-positive cells were single-cell sorted into 96-well plates using a MoFlo Astrios Cell Sortor (Beckman Coulter) and cultured for another 2~3 weeks to allow for clonal expansion ...
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bioRxiv - Cell Biology 2020Quote: ... and YFP+ cells isolated using fluorescence activated cell sorting (FACS) with a MoFloXDP cell sorter (Beckman Coulter, Wycombe, UK). YFP+ cells were selected using a 530/40 filter set.
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bioRxiv - Bioengineering 2022Quote: ... Cell pellet was resuspended in assay medium and counted using an automated cell counter (Vi-Cell XR, Beckman Coulter). Cells were then diluted to a concentration of 1×10^6 cells/mL in assay medium and then activated using 1:500 TransAct ...
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bioRxiv - Cell Biology 2024Quote: ... mTurquoisehigh cells were sorted into 96-well plates at 1 cell/well using a MoFlo Cell Sorter (Beckman Coulter). For cells transfected with sgRNA/Puro plasmids ...
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bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation (12,000x□g for 15□min at 4°C) and membranes were then pelleted at 45,000 rpm in a Type 45 Ti rotor (Beckman) for 3□h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40 000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (25 000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was incubated with GSH beads (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... after which the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA 25.50 rotor in an Avanti J-E centrifuge (Beckman). Nucleic acids in the clarified lysate were precipitated by slowly adding polyethyleneimine with constant stirring in an ice bath to a final concentration of 0.4% ...
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bioRxiv - Microbiology 2020Quote: ... of 1.5 mL and then centrifuged 45 min at 100,000g at 4°C in a TLA-100 ultracentrifuge (Beckman Coulter). The supernatant contains the soluble fraction and the pellet corresponds to the total membranes ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting gradients were subjected to ultracentrifugation at 4°C for 14 hrs at 41,000 rpm in an SW41 Ti rotor (Beckman), and then fractionated ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 mM DTT, 100 μg/ml cycloheximide) and centrifuged (160,000 g, 120 min, 4°C) in an SW41Ti rotor (Beckman). After centrifugation the sediment in the tube went through a UV detector at the wavelength of 254nm and simultaneously the required fractions (>=5 ribosomes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The filtered supernatant from one 400 ml batch was then pelleted (1.5 hour at 24 000 rpm at 4 °C with Beckman Coulter L-90K centrifuge and 45 Ti rotor ...
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bioRxiv - Biophysics 2022Quote: ... The sample was loaded on top of the glycerol gradient and centrifuged at 4°C for 18 h at a speed of 35,000 rpm in SW-41Ti rotor (Beckman). Fractions of 500 μL were collected ...
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bioRxiv - Cell Biology 2019Quote: ... Sucrose gradients were subjected to ultracentrifugation (SW41 Ti 11E1698 rotor; Beckman at 260,000xg for 2 h at 4□°C) and fractionated by displacement using 60% sucrose/0.01% bromophenol blue on an ISCO Foxy fraction collector (35 s for each fraction ...
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bioRxiv - Molecular Biology 2019Quote: ... The 80S fraction was pelleted for 1 h at 436000 x g at 4 °C (TLA-100, Beckman Coulter), resuspended in 20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Phage were pelleted by centrifugation at 82705 xg (25,000 rpm) for 4 hours using an SW28Ti rotor (Beckman Coulter). Phage pellets were gently resuspended overnight in 1 mL of phage buffer at 4°C with shaking ...
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bioRxiv - Genomics 2021Quote: ... we filtered the virus-laden media through a 0.22μm PES filter and then concentrated viral particles by ultracentrifugation at 24,000 rpm for 1.5 hours (4°C) with an SW-32Ti swinging bucket rotor (Beckman Coulter). We resuspended the viral pellet in iPS-Brew at 1/200th the original supernatant volume and then stored 50-100 μl aliquots at −80°C.
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bioRxiv - Cell Biology 2020Quote: ... Lysates were loaded on the top of the gradient and centrifuged at 260,000 × g and 4 °C for 2 h using SW 41 rotor (Beckman). Collection of polysomal fractions were done using Piston Gradient Fractionator (BioComp) ...
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bioRxiv - Microbiology 2021Quote: ... 200mM NaCl was used to top up the 13mL polyethylene tube and the solutions were spun at 27,000×g for 18 hours at 4°C using SW45 Ti rotor (Beckman). 1mL of top fraction ...
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bioRxiv - Biochemistry 2020Quote: ... and were centrifuged for 4 min at 46 000 rpm at 4°C in a Optima MAX-XP Ultracentrifuge with a TLA 100.3 rotor (Beckman). The pellet was suspended in (20 µl ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were further amplified 4–6 PCR cycles as described [43] and purified using AMPure XP beads (Beckman Coulter). ATAC-seq libraries were subsequently sequenced 2 x 75 bp on an Illumina NextSeq500.
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bioRxiv - Biophysics 2021Quote: ... sucrose density gradients and centrifuged at 24,000 × rpm (102,170 × g) for 18 h at 4°C (Sw40Ti rotor, Beckman Coulter). The fractions of the sucrose gradient were applied to SDS-PAGE ...
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bioRxiv - Biophysics 2021Quote: ... The VP2 capsid protein fractions were pooled and subjected to centrifugation at 28,000 rpm (139,065 × g) for 3 h at 4°C (Sw40Ti rotor, Beckman Coulter). The pellet was resuspended in 50 mM Tris (pH 7.4) ...
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bioRxiv - Biochemistry 2020Quote: ... mixed for 10 minutes at 4°C and centrifuged at 25,000 rpm for 45 minutes (Beckman Ti-45 rotor). The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 97,658 x g for 3 h at 4°C using a SW41Ti rotor (Beckman & Coulter, # 331362). The polysome fractions were collected using the Foxy R1 Fraction Collector and immediately snap frozen with liquid nitrogen and stored at −80 °C ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 40,000 rpm (∼128,000 × g) for 3 h at 4°C using an MLS-5 rotor (Beckman Coulter). Aldolase (158 kDa ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) by being centrifuged at 73000 rpm for 45 minutes at 4°C (Beckman Coulter TL120.2 rotor). The mitoribosomal pellet was subsequently resuspended in ribosome resuspension buffer (25 mM HEPES-KOH pH = 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... 1□mM EDTA) and centrifuged at 25000 rpm for 1 hour at 4°C (Beckman Coulter SW41-Ti rotor). Mitochondria forming a band at the interphase between the 1 M and 1.5 M sucrose were collected and resuspended in 10 mM Tris-HCl pH = 7.5 in 1:1 ratio ...
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bioRxiv - Biochemistry 2020Quote: ... Cell debris was removed by centrifugation at 14,800 rpm for 20 minutes 4 °C in F241.5P rotor using 149 Microfuge 22R centrifuge (Beckman Coulter). The supernatant was combined with 100 μL of ANTI-FLAG M2 Affinity Gel (Sigma ...