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Citations for Beckman :
1 - 50 of 251 citations for Dengue Virus Serotype 3 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
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bioRxiv - Immunology 2021Quote: ... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
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bioRxiv - Microbiology 2024Quote: ... The virus was enriched by ultracentrifugation (Beckman Coulter Optima™ L-80XP) at 246,347g for 4 hours at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... the virus particles were pelleted by ultracentrifugation in a Beckman type 19 rotor (Beckman Coulter ...
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bioRxiv - Synthetic Biology 2020Quote: Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 28) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor) for 2h at 4 °C ...
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bioRxiv - Systems Biology 2021Quote: HeLa Kyoto and R1/E mESCs (gifts from Hyman lab, Dresden) and HEK293 (gift from Beckman lab, Berlin), Ptk2 cell lines (gift from Simons lab ...
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bioRxiv - Synthetic Biology 2019Quote: ... Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 32 Ti) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Virus was separated from free dye by ultracentrifugation as above on a SW60Ti rotor (Beckman Coulter). The virus band was extracted and desalted on a NAP column (VWR ...
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bioRxiv - Immunology 2021Quote: Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter). Test sera were diluted 1:40 in growth medium and serially diluted ...
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bioRxiv - Biochemistry 2021Quote: ... The virus-containing filtrate was then further purified using an ultracentrifuge (Optima XPN-90 Ultracentrifuge, Beckman Coulter, USA) with a cycle of 1,000 rpm (116 g ...
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bioRxiv - Immunology 2021Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
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bioRxiv - Microbiology 2022Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
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bioRxiv - Microbiology 2020Quote: ... The virus was then purified over a 20% sucrose cushion by centrifugation at 35,000 rpm (Beckman Coulter SW41 rotor) for 3 h at 4°C ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...
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bioRxiv - Molecular Biology 2020Quote: ... Virus particles in the supernatant were pelleted by ultracentrifugation at 45.000 rpm for 45 min (Beckman 70.1 Ti Rotor) over a pad of 15% sucrose in 10 mM Tris-HCl buffer (pH 7.4) ...
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bioRxiv - Microbiology 2024Quote: ... Virus RNA was extracted with a magnetic bead-based RNA extraction kit (RNAdvance Viral Reagent kit, Beckman Coulter, C63510) on an epMotion® 5073t liquid handler (Eppendorf ...
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bioRxiv - Cell Biology 2022Quote: ... the virus-cell mixture was centrifuged at 1,000 rcf for 2 hours at 32°C (Beckman Coulter, Allegra X-30R). Infected cells were then cultured as described above and subsequently FACS-sorted for the establishment of clonal cell lines.
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bioRxiv - Biophysics 2023Quote: ... Virus-containing supernatants were collected at 48 hours and 72 hours after transfection and concentrated using ultracentrifugation (Ultraclear, Beckman 344058). The transduction capacity of the viral vector product was confirmed by culturing non-transfected HEK293T cells with the virus after 24 hours and assessing via fluorescent imaging.
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bioRxiv - Microbiology 2022Quote: ... Supernatant was collected and virus pelleted through 30% (w/v) sucrose cushion at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 hours at 10°C ...
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bioRxiv - Immunology 2020Quote: ... KIR2DL2/3/S2 (GL183, PE/Cy5.5, Beckman Coulter,), NKG2C (134591 ...
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bioRxiv - Systems Biology 2023Quote: ... enumerated using a Multisizer 3 Coulter Counter (Beckman,), and then seeded at the desired density in 6-well tissue culture plates (TPP ...
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bioRxiv - Biochemistry 2020Quote: ... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
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bioRxiv - Immunology 2021Quote: ... CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1 ...
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bioRxiv - Immunology 2020Quote: ... recombinant protein and CD14 antibody (Beckman) were used at 20 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: Proteins were collected by centrifugation (Beckman-Coulter JA-14 rotor ...
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bioRxiv - Plant Biology 2019Quote: ... or 3) using a Coulter counter (Beckman Coulter Multisizer 4e) fitted with a 70 µm aperture tube ...
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bioRxiv - Developmental Biology 2020Quote: ... a CytoFLEX 3 laser 13 colour flow cytometer (Beckman Coulter) was used ...
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bioRxiv - Cell Biology 2020Quote: ... Protein aggregates were pelleted by ultracentrifugation of the proteins individually at 80,000 rpm in TLA 100 (Beckman Coulter) for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... recombinant protein and anti-CD14 antibody (Beckman) were used at 20 μg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... 100,000xg for 3 h in an Optima XPN-80 (Beckman Coulter) ultracentrifuge using UltraClear Thinwall tubes ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 °C for 3 h (SW40 Ti rotor, Beckman-Coulter, Germany), the supernatant was discarded ...
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bioRxiv - Immunology 2023Quote: ... Lymphocytes were enumerated with a cell counter (Beckman Coulter Multisizer 3) set to detect nuclei between 3.5 and 10 μm ...
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bioRxiv - Immunology 2023Quote: ... Lymphocytes were enumerated with a cell counter (Beckman Coulter Multisizer 3) set to detect nuclei between 3.5 and 10 μm.
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bioRxiv - Immunology 2023Quote: ... Measurements were performed in a Gallios 10/3 cytometer (Beckman Coulter), using the Kaluza for Gallios Acquisition software (Version 1.0 ...
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bioRxiv - Immunology 2023Quote: ... AlexaFluor 700 anti-human CD38 (clone LS198-4-3, Beckman Coulter), APC-Cy7 anti-human CD19 (clone SJ25C1 ...
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bioRxiv - Biochemistry 2024Quote: ... 4 °C for 3 h using a SW41 rotor (Beckman Coulter). Sucrose gradients were fractionated using a Piston Gradient Fractionator (Biocomp) ...
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bioRxiv - Biochemistry 2024Quote: ... 4 °C for 3 h using a SW41 rotor (Beckman Coulter). Sucrose gradients were fractionated using a Piston Gradient Fractionator (Biocomp) ...
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bioRxiv - Biophysics 2022Quote: ... Ribosomes were isolated by ultracentrifugation for 3 hours at 50,000 rpm (Beckman-Coulter Optima-90 ...
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bioRxiv - Genomics 2019Quote: DNA fragments (3~8 kb) were purified with Agencourt AmpureXP beads (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... Samples were measured on a Gallios (10 colors, 3 lasers, Beckman Coulter) flow cytometer using FACS Kaluza software v2.1.1 (Beckman Coulter) ...
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bioRxiv - Cell Biology 2022Quote: ... and cell-size distribution measurements with a Coulter Counter (Beckman Multisizer 3) were conducted as described previously (42) ...
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bioRxiv - Physiology 2023Quote: ... and monitored twice daily using an electronic particle counter (Multisizer 3, Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell counts were determined using a Multisizer 3 Coulter Counter (Beckman Coulter) with a diameter setting of 10-30 μm ...
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bioRxiv - Cell Biology 2023Quote: ... followed by cell counting using a multisizer 3 coulter counter (Beckman Coulter), according to the manufacturer’s instructions41 ...
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bioRxiv - Physiology 2024Quote: ... and particle diameter measured using a Multisizer 3 Coulter Counter (Beckman Coulter). A 100 µm aperture was used for all experiments ...
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bioRxiv - Cell Biology 2021Quote: ... final cell counts were measured using a Multisizer 3 Coulter Counter (Beckman Coulter). The following formula was used to calculate proliferation rate:
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bioRxiv - Cancer Biology 2020Quote: ... 3 and 5 and counted using a particle counter (Z1; Beckman Coulter, Inc.). Experiments were carried out in triplicate ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed 3 times and analyzed with MoFlo (Beckman, Brea, CA, USA) and associated software ...
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bioRxiv - Cell Biology 2020Quote: Size of HSCs was determined using a Multisizer-3 Coulter Counter (Beckman Coulter) or microscopy ...