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Citations for Beckman :
401 - 450 of 1842 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and either aliquoted or concentrated by ultracentrifugation using 20% (w/v) sucrose cushions for 2 hours at 25,000 rpm in an SW41 rotor (Beckman).
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bioRxiv - Cell Biology 2022Quote: ... milk was prevented from cooling down and was processed within 20 minutes after collection by 2 rounds of centrifugation at 3,000xg for 10 minutes at 23°C (Beckman Coulter Allegra X-12R ...
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bioRxiv - Microbiology 2022Quote: ... The pellet was resuspended in 2 ml (per fraction) of the supernatant and ultracentrifuged (Optima MAX-TL, Beckman Coulter) at 12,000 rpm (TLA55 rotor ...
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bioRxiv - Molecular Biology 2023Quote: ... The gradients were centrifuged at 38,300 rpm for 2 hours and 45 min at 4 °C in a SW-40Ti (Beckman) rotor using an Optima XE-100 Ultracentrifuge (Beckman) ...
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bioRxiv - Molecular Biology 2023Quote: ... The gradients were centrifuged at 38,300 rpm for 2 hours and 45 min at 4 °C in a SW-40Ti (Beckman) rotor using an Optima XE-100 Ultracentrifuge (Beckman) ...
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bioRxiv - Plant Biology 2023Quote: ... Extract was then centrifuged at 40 000 rpm for 2 hours at 4°C using the 50Ti rotor (Beckman). A protocole previsouly used in the laboratory and described in Maclot et al ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was subjected to ultracentrifugation at 200,000 xg for 2 h (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA clean-up was performed by incubating RNA solution in 2 volumes of AMPure XP Beads (Beckman Coulter, A63881) for 2 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-1.5 μg of total protein was loaded onto a 10% to 50% sucrose gradient and centrifuged at 36,000 rpm for 2 hours at 4°C using an SW41 rotor (Beckman). Polysome profiles were visualized ...
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bioRxiv - Microbiology 2023Quote: ... 500 μ g of the lysate was loaded on the top of 15–50% sucrose gradient containing 100μ g/ml cycloheximide and the gradients were centrifuged at 36,000 rpm for 2 h at 4°C in SW41 rotor (Beckman). Density Gradient Fractionation System (ISCO ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... The samples were centrifugated at 70000 rpm for 2 h at 4°C in an Optima TLX Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Genetics 2023Quote: ... Fifteen A260 units of WCEs were layered on a pre-chilled 10-50% sucrose gradient and centrifuged at 40,000 rpm for 2 h at 4°C in a SW41Ti rotor (Beckman). Gradient fractions were continuously scanned at A260 using the BioComp Gradient Station and polysome to monosome (P/M ...
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bioRxiv - Cancer Biology 2023Quote: ... the obtained supernatant was filtered through a 0.22 μm filter before ultracentrifugation at 150,000 g for 2 h at 4 °C (Beckman Coulter). The nsEV-containing pellet was resuspended in phosphate buffer saline (PBS).
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bioRxiv - Neuroscience 2023Quote: ... Samples were then centrifuged at 82,600×g at 4°C for 2 h (Beckman ultracentrifuge optima L-100 XP). After the first ultracentrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... samples were ultracentrifuged at 160,000 xg for 2 h at 4 °C in ultracentrifuge tubes (Beckman Coulter Cat #328874). The resulting pellet contained the insoluble AMB-1 cell fraction and the supernatant contained the soluble fraction ...
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bioRxiv - Neuroscience 2023Quote: ... The tubes were centrifuged at 100,000 × g for 2 hours using a SW 55Ti swinging-bucket rotor (Beckman Coulter).
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was then subjected to ultracentrifugation at 100,000 x g for 2 h at 4 °C to sediment small EVs (Optima L-80K ultracentrifuge, Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 μg/mL cycloheximide) for 2 h at 40,000 g at 4 °C using an SW41 rotor (Beckman Coulter). Gradients were fractionated using Teledyne Isco Tris Peristaltic Pump and fractions were collected and pooled according to the UV trace ...
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bioRxiv - Cell Biology 2020Quote: ... mCherry-positive cells were individually sorted into 96-well plates using a MoFlo Cell Sorter (Beckman Coulter). The presence of frameshift-causing insertion/deletions in both alleles of the obtained clones was achieved by capillary electrophoresis on a 3730xl DNA analyser (Applied Biosystems ...
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bioRxiv - Cancer Biology 2019Quote: ... Viable cell counts at each time point were performed using a Vi-Cell XR counter (Beckman Coulter) and calculated for each cell type in separate and co-culture by applying the percentages from the flow cytometry analysis.
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bioRxiv - Cell Biology 2020Quote: ... ESCs were single cell sorted in 96-well plates using a MoFlo XDP cell sorter (Beckman Coulter) and subsequently expanded.
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bioRxiv - Genomics 2022Quote: ... Each single-cell suspension cell concentration was counted using Flow-count fluorospheres (Beckman Coulter, Brea, CA, USA) and a Gallios flow cytometer (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD25-CD44- CD45RBhi cells were sorted with the MoFlo Astrios cell sorter (Beckman Coulter, Indianapolis, IN) and intraperitoneally injected into Rag-1-/- recipients.
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bioRxiv - Cancer Biology 2021Quote: ... Viable cell number at each time point was quantified by using a Vi-Cell counter (Beckman-Coulter), and % survival was calculated relative to viable count before drug exposure (t=0) ...
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bioRxiv - Cancer Biology 2022Quote: ... Bone marrow cell numbers and viability were determined using an automated cell counter (ViCell Blu, Beckman Coulter). Spleen cell suspensions were generated by crushing whole spleen and filtering through a 70 μM filter ...
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bioRxiv - Cancer Biology 2021Quote: ... Total cell counts were determined from purified mononuclear cell suspensions using a DxH500 Hematology Analyzer (Beckman Coulter). Purified cell suspensions were stained for 30 - 40 minutes at 4°C in the dark with a master mix of the respective antibodies followed by a washing step in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and cell numbers were counted using either a Hemocytometer or the ViCell Cell Viability Analyzer (Beckman Coulter).
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bioRxiv - Cell Biology 2020Quote: ... Cells were sorted on a MoFlo Astrios high speed cell sorting device (Beckman-Coulter, Brea, CA, USA), and only DAPI-negative cells ...
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bioRxiv - Cell Biology 2020Quote: ... Resistant cells were then single cell sorted into 96-well plates using a MoFlo Astrios (Beckman Coulter) to begin clonal cell lines.
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bioRxiv - Immunology 2020Quote: Single cell RNA was purified from individual B cells using magnetic beads (Beckman Counter, RNAClean XP, A63987) and used for cDNA synthesis by reverse transcription (SuperScript III Reverse Transcriptase ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-positive C20 microglia were sorted as single cells using the MoFlo XDP Cell Sorter (Beckman-Coulter). Successful ATM KO clones were identified by fragment analysis using capillary electrophoresis (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... cell numbers and cell volumes were determined using Z2 Coulter particle count and size analyzer (Beckman Coulter) to calculate total cell volume for each sample ...
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bioRxiv - Immunology 2022Quote: ... Cell numbers of each cell subset were evaluated by using Flow-Count Fluorospheres (Beckman Coulter, Brea, CA) and presented as numbers per tissue after exclusion of doublet and dead cells.
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bioRxiv - Cell Biology 2023Quote: ... and cells with the same fluorescence intensities were collected by a cell sorter (MoFlo AstriosEQ; Beckman Coulter).
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bioRxiv - Cell Biology 2023Quote: ... 10% of the sorted cells were used to measure cell size using a Z2 Coulter counter (Beckman), and the rest of the cells were lysed in RIPA lysis buffer supplemented with protease inhibitors and phosphatase inhibitors ...
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bioRxiv - Cell Biology 2023Quote: ... Multiple-organelle marker co-expressing cells were isolated using a cell sorter (MoFlo Astrios EQ, Beckman Coulter).
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bioRxiv - Neuroscience 2023Quote: ... cells were dissociated and sorted using the C.SIGHT clonal dispenser (Cytena) or the MoFlo cell sorter (Beckman) in 96-well plates ...
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bioRxiv - Molecular Biology 2022Quote: ... were pre-clarified to remove aggregate proteins by spinning at 65,000 rpm for 30 min at 4°C in a TLA100 rotor (Beckman Coulter, Brea, CA) before incubating with F-actin ...
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bioRxiv - Cell Biology 2019Quote: ... The obtained supernatant (containing membrane and cytosolic proteins) was then centrifuged at 180,000 × g for 90 min at 4°C using a TLA-55 rotor (Beckman Coulter, Brea, CA). The obtained pellet (containing intact membranes ...
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bioRxiv - Plant Biology 2021Quote: ... Cell density and mean cell diameter were measured using a Coulter Counter (Multisizer 3, Beckman Counter, Brea, CA). For the data in Figure 1A ...
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bioRxiv - Cell Biology 2020Quote: ... and cell size and cell density were measured using the Multisizer 4 Coulter Counter (Beckman-Coulter, Mississauga, ON) or collected for whole cell lysis at time points indicated in the figures ...
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bioRxiv - Cancer Biology 2022Quote: ... transduced cells were selected using 1μg/mL puromycin (LabForce) or sorted on an Astrios cell sorter (Beckman Coulter) dependent on the vector.
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bioRxiv - Developmental Biology 2022Quote: ... CD31+TdTomato+ cells underwent FACS-based isolation from single cell suspension using a MoFlo Astrios EQ (Beckman Coulter) flow cytometer with antibody staining for CD31-PECy7 (25-311-82 ...
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bioRxiv - Cancer Biology 2019Quote: ... GFP-negative cancer cells were sorted using the MoFlo XDP cell sorter with a 100μm nozzle (Beckman Coulter) as part of the University of Colorado Cancer Center Flow Cytometry Shared Resource ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Then the cells were sorted into empty 96-well plates using MoFlo Astrios EQ cell sorter (Beckman Coulter). Gates were set mainly based on Mitotracker intensity and the dye was detected by a 488 nm and 640 nm laser for excitation ...
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bioRxiv - Immunology 2020Quote: ... Unstimulated cells and pokeweed mitogen-stimulated cells were additionally stained with AF750 anti-CD19 (Beckman Coulter, clone J3119). Samples were incubated 15 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: Cells were analysed by flow cytometry for GFP expression or cell surface staining using Gallios (Beckman Coulter, UK), Attune (Thermo ...
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bioRxiv - Immunology 2023Quote: ... White blood cell counts were determined from full blood with an automatic cell counter (DxH 500, Beckman Coulter). For bioluminescent imaging ...
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bioRxiv - Plant Biology 2023Quote: ... Cell density and mean cell volume were measured using a Coulter Counter (Multisizer 3, Beckman Counter, Brea, CA). For 35°C with photoautotrophic medium ...