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Citations for Beckman :
1 - 50 of 716 citations for DIRAS Family GTP Binding RAS Like 1 DIRAS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... 1 mM GTP in a TLA110 rotor (Beckman, Palo Alto, CA) at 80,000 rpm ...
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bioRxiv - Immunology 2022Quote: The TCR V beta repertoire kit contained antibodies to over 76% of V βeta families (Beckman Coulter, Fullerton, CA). The cells were stained with these antibodies in combination with tetramers or dextramers for 20 minutes at RT to determine the TRBV repertoire of antigen specific cells ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 mM GTP) layered in TLS-55 (Beckman) centrifuge tubes ...
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bioRxiv - Genomics 2022Quote: ... Phosphate Buffered Saline-like (Puraflow Sheath Fluid, Beckman Coulter) was used as sheath and run at a constant pressure of 10 or 25 PSI ...
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bioRxiv - Immunology 2020Quote: ... Antibody binding was analyzed by flow cytometry (FACS CytoFLEX; Beckman Coulter®) by determination of the mean fluorescence intensity (MFI ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with GTP) was thawed and spun in an ultracentrifuge (Beckman Coulter OptimaMax) at 70k rpm for 5 min ...
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bioRxiv - Immunology 2021Quote: ... and Treg-like cells sorted as CD19- TCRβ+CD4+Thy1.1-GFP+ on a Moflo Astrios (Beckman Coulter).
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bioRxiv - Bioengineering 2024Quote: ... the stained cells were washed twice to remove any unspecific binding antibodies and were then subjected to measurement using a Beckman Cytoflex S flow cytometer (Beckman, CA, USA). The resulting data were analyzed using FlowJo software (Tree Star ...
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bioRxiv - Immunology 2022Quote: ... Fibronectin binding was assessed by flow cytometry (CytoFlex, Beckman Coulter). GFP-expressing bacteria were assessed by gating for FITC positive events ...
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bioRxiv - Microbiology 2024Quote: ... Binding was analyzed by flow cytometry (Cytoflex S, Beckman Coulter) by comparing the TAMRA-signals (PE-channel ...
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bioRxiv - Molecular Biology 2020Quote: Binding to CD16 was studied by a competitive assay with an anti-CD16-PE/3G8 antibody conjugated to phycoerythrin (Beckman Coulter, Brea, CA, USA) and unlabelled test and control antibodies using Jurkat-CD16 cells.
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bioRxiv - Microbiology 2023Quote: ... The AMPure XP binding beads (Beckman Coulter Genomic, Indianapolis, IN, USA).
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bioRxiv - Molecular Biology 2019Quote: ... The RNA was purified by binding to Ampure XP beads (Beckman Coulter) by addition of 2 volumes of beads ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with an additional 400 µl 1× Binding Buffer and analyzed within 20 min by CytoFLEX S (Beckman Coulter Life Science). At least 1 × 104 cells were analyzed to determine the percentage of apoptotic cells ...
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bioRxiv - Bioengineering 2023Quote: ... cells were analyzed for target binding using a CytoFLEX flow cytometer (Beckman Coulter). Background-subtracted and normalized binding curves were fitted to a first- order binding model and equilibrium dissociation constant (KD ...
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bioRxiv - Cell Biology 2021Quote: ... by binding samples with a 1.5 X volume of AMPure XP beads (Beckman Coulter), washing twice with 70 % ethanol ...
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bioRxiv - Microbiology 2021Quote: ... the samples were resuspended in binding buffer and analysed using a cytofluorimeter (CytoflexS, Beckman) with excitation at 488 nm and 525 nm emission for Annexin and 585 nm emission for PI ...
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bioRxiv - Biochemistry 2024Quote: ... Annexin V and PI binding were analyzed using an CytoFLEX flow cytometer (Beckman Coulter), and the percentage of apoptotic cells was calculated from the total number of cells (∼1 × 104 cells ...
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bioRxiv - Genetics 2021Quote: ... The amplified libraries were pooled and purified again by binding to AMPure XP (Beckman-Coulter #A63880) beads (0.9 beads:1 sample) ...
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bioRxiv - Genetics 2019Quote: ... and sex hormone binding globulin (SHBG) were determined by a chemiluminescent immunoassay (Beckman Coulter DXI 800). The Immuno-turbidimetric method (Beckman Coulter DXI 800 ...
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bioRxiv - Microbiology 2023Quote: ... the PCR products were cleaned using AMPure XP binding beads (Beckman Coulter Genomic, Indianapolis, IN, USA). The AMPure XP binding beads (Beckman Coulter Genomic ...
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bioRxiv - Microbiology 2021Quote: ... using anti-HIV-1 p24-PE antibody (#6604667, 1:1000 dilution) purchased from Beckman Coulter ...
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bioRxiv - Cancer Biology 2020Quote: ... 200 μL of 1x binding buffer were added and suspension was analyzed by flow cytometry (Beckman-Coulter, CytoFLEX). Flow cytometry gates were chosen based on untreated controls for each cell line and values were normalized to controls.
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bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in p24 primary antibody solution (1:100 dilution of p24-FITC antibody clone KC57 (Beckman Coulter) diluted in perm/wash buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The library was cleaned up and size-selected by two consecutive rounds of binding to 1.2x and 0.8x SPRI beads (Agentcourt AMPure XP beads, Beckman Coulter). The integrity and size distribution of the final libraries (two biological replicates of KCl-treated and one of untreated ...
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bioRxiv - Genomics 2021Quote: ... Estradiol (E2) and Progesterone assays are competitive binding immunoenzymatic assays performed on the Unicel DXI 800 system (Beckman Coulter). FSH assay is based on Microparticle Enzyme Immunoassay (MEIA ...
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bioRxiv - Microbiology 2022Quote: ... 400 μL of 1X binding buffer was then added before analysing using a CytoFLEX S flow cytometer (Beckman Coutler). Annexin V-FITC positive cells were designated as early apoptotic ...
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bioRxiv - Biochemistry 2023Quote: ... binding reactions were centrifuged at 50,000 RPM for 30 min at 25 °C in a TLA-100 rotor (Beckman). Promptly after centrifugation ...
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bioRxiv - Biochemistry 2023Quote: ... binding reactions were centrifuged at 50,000 RPM for 30 min at 25 °C in a TLA-100 rotor (Beckman). Following centrifugation ...
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bioRxiv - Immunology 2021Quote: ... Antibodies against the following molecules were included: CD49d (HP2/1; Beckman Coulter), CD32 (FUN-2 ...
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bioRxiv - Immunology 2023Quote: ... and intracellularly stained with the Fluorescein isothiocyanate-labeled KC57 (anti-HIV-1 p24) antibody (1:200) (Beckman Coulter) or a mixture of both antibodies ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with 1% FBS in PBS antibody staining buffer and stained with the following antibodies: CD64 PE-Cy7 (Cat. # B06025, Beckman Coulter); CD45 PerCP (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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bioRxiv - Genomics 2023Quote: Sheared DNA was purified by binding to an equal volume of AMPure XP beads (A63881, Beckman Coulter, Inc. Brea, CA) and eluted in 50µl of 10mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... and p24 antibodies (anti-HIV core antigen: clone KC57, Beckman Coulter, 1:200).
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bioRxiv - Microbiology 2021Quote: Antibodies used for flow cytometry: anti-HIV-1 p24-PE (KC57-RD1 Beckman Coulter); anti-HIV-1 Env clone PGT151 (gift from Laura McCoy ...
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bioRxiv - Biochemistry 2021Quote: ... Binding reactions were spun at 300,000 g for 30 min at 25 °C using Optima MAX-TL ultracentrifuge (Beckman Coulter, Brea, CA). Bundling reactions were spun at 17,000 g for 15 min at 25 °C ...
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bioRxiv - Immunology 2022Quote: ... following manufacturer’s instructions with the following p24 antibodies: HIV-1 p24 clone 28B7-APC (Medimabs) and HIV-1 p24 clone KC57-PE (Beckman Coulter). Acquisition was performed on the Fortessa LSR flow cytometer (BD Bioscience ...
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bioRxiv - Cancer Biology 2020Quote: ... 320 μL of 1X binding buffer was added to each sample before analysis on the EPICS XL flow cytometer (Beckman Coulter, Buckinghamshire, UK).
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bioRxiv - Immunology 2020Quote: ... HIV-1 p24-PE antibody (KC57-RD1, #6604667) for intracellular staining was purchased from Beckman Coulter ...
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bioRxiv - Cell Biology 2019Quote: ... Intracellular proteins were then stained with anti-Gag KC57 RD1 antibody (1/100, Beckman Coulter) and/or anti-α-tubulin (5 μg/mL ...
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bioRxiv - Immunology 2021Quote: ... following by staining for 15 minutes at room temperature with a 1:100 dilution of a fluorescent anti-HIV-1 GAG antibody (KC57-FITC, Beckman Coulter reference 6604665) in permeabilization buffer ...
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bioRxiv - Microbiology 2021Quote: ... 50 μL of each cell sample was mixed with 1 μL of KC57-FITC antibody that identifies the HIV-1 Gag and p24 (Beckman Coulter Life Sciences, USA) and incubated at 4°C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... a fluorescent labeling was first made with anti-p24 antibody (Clone: KC57, Beckman Coulter, 6604667; 1/200 dilution). Then ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with the fluorescently labeled anti-p24 antibody (Clone: KC57, Beckman Coulter, 6604667; 1/200 dilution).
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bioRxiv - Immunology 2021Quote: ... Cells were stained with following antibody mix for 30 minutes on ice: pan-γδTCR-PE (1:10; IMMU510; Beckman Coultier) and CD69-APC (1:25 ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated with fluorophore-conjugated antibodies for 30min (see Supplementary Table 1) and FACS-sorted using MoFlo Legacy or Astrios cell sorters (Beckman Coulter). Cells were sequentially gated based on size and granularity (forward scatter ...
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bioRxiv - Immunology 2019Quote: ... CECs were pre-incubated with human recombinant CCL5 (1-1000 ng/ml, R&D) and/or CD35 blocking antibody (5-20 μg/ml, clone J3D3, Beckman Coulter) for an hour prior to infection.
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bioRxiv - Biochemistry 2021Quote: ... live cells were stained with Alexa 647-conjugated M1 anti-FLAG antibody (1:1000, 5 minutes) and analyzed on a Cytoflex flow cytometer (Beckman Coulter).
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% FA in PBS for 90 min at room temperature and immunostained for intracellular HIV-1 CA for 30 min at 4°C using KC57-FITC antibody (Beckman Coulter) diluted in 0.1% Triton X-100 ...