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1 - 50 of 532 citations for Cytosolic arginine sensor for mTORC1 subunit 2 CASTOR2 Antibody FITC since 2020

• Induction of NASH in the Nwd1^−/− mouse liver via SERCA2-dependent endoplasmic reticulum stress

  Seiya Yamada, et al., bioRxiv - Molecular Biology 2024

  Quote: ... The cytosolic fraction was further separated into an ER-enriched pellet and cytosolic supernatant by ultracentrifugation (Optima LE-80K, Beckman Coulter, Brea, CA, USA) at 100,000 × g for 1 h at 4°C ...
• Cone-shaped HIV-1 capsids are transported through intact nuclear pores

  Vojtech Zila, et al., bioRxiv - Microbiology 2020

  Quote: ... fluorescein isothiocyanate (FITC)-conjugated mouse monoclonal antibody KC57 (Beckman Coulter) was used at a dilution of 1:100.
• HIV-1 infection causes depletion of monocytic cells through a non-canonical cell death pathway

  Michelle Stuck, et al., bioRxiv - Microbiology 2022

  Quote: ... fixed cells were incubated with FITC-p24 antibody (KC57; Beckman Coulter) in the presence of 0.1% Triton-X ...
• The DEAD box RNA helicase DDX42 is an intrinsic inhibitor of positive-strand RNA viruses

  Boris Bonaventure, et al., bioRxiv - Microbiology 2021

  Quote: ... and intracellular staining with the anti-p24Gag KC57-FITC antibody (Beckman Coulter), as described previously (67) ...
• An Environmental Restriction impairs HIV-1 virion fusion and triggers innate immune recognition

  Samy Sid Ahmed, et al., bioRxiv - Immunology 2023

  Quote: ... then stained with an anti-p24 KC-57 FITC antibody (Beckman Coulter) according to manufacturer protocol ...
• An Environmental Restriction impairs HIV-1 virion fusion and triggers innate immune recognition

  Samy Sid Ahmed, et al., bioRxiv - Immunology 2023

  Quote: ... then stained with an anti-p24 KC-57 FITC antibody (Beckman Coulter) according to manufacturer protocol ...
• Pseudomonas aeruginosa MipA-MipB envelope proteins act as new sensors of polymyxin

  Manon Janet-Maitre, et al., bioRxiv - Microbiology 2023

  Quote: ... Cytosolic and membranes components were separated by ultracentrifugation at 200,000 xg (Rotor TLA120 Beckman) for 45 min at 4°C ...
• In-depth single-cell analysis of translation-competent HIV-1 reservoirs identifies cellular sources of plasma viremia

  Basiel Cole, et al., bioRxiv - Microbiology 2021

  Quote: ... we used a combination of two antibodies: p24 KC57-FITC (Beckman Coulter, 6604665) and p24 28B7-APC (MediMabs ...
• Pooled genetic screens identify breast cancer risk genes involved in evasion from T cell-mediated killing

  Wei Shi, et al., bioRxiv - Genetics 2024

  Quote: ... The panel included antibodies for TCR Vβ8 FITC (clone 56C5.2, Beckman Cat# IM1233), CD3 BV711 (clone SK7 ...
• Intravenous haloperidol and cocaine alter the distribution of T CD4⁺ and B lymphocytes and NKT cells in rats

  Maciej M. Jankowski, et al., bioRxiv - Pharmacology and Toxicology 2021

  Quote: ... Three-color flow cytometry of whole blood samples and diluted suspension of splenocytes was performed using the following antibodies: IOTest CD3-FITC/CD45RA-PC7/CD161a-APC and CD3-FITC/CD4-PC7/CD8-APC (Beckman Coulter, Immunotech, Marseille, France) for determination of T ...
• Diacylglycerol kinase ζ deficiency triggers early signs of aplastic anemia in mice

  M Martín-Salgado, et al., bioRxiv - Immunology 2020

  Quote: ... CD44-FITC (Beckman) and CD69-PE (Pharmingen) ...
• A dual ribosomal system in the zebrafish soma and germline

  Arish N. Shah, et al., bioRxiv - Developmental Biology 2024

  Quote: ... Ribosomal subunits were pelleted using a TLA100.3 rotor (Beckman Coulter, 349490) at ∼300,000 xg at 4 °C for 2 h ...
• Comparative Analysis of T Cell Spatial Proteomics and the Influence of HIV Expression

  Aaron L. Oom, et al., bioRxiv - Cell Biology 2021

  Quote: ... Cells were resuspended in p24 primary antibody solution (1:100 dilution of p24-FITC antibody clone KC57 (Beckman Coulter) diluted in perm/wash buffer ...
• CD4+ T cells facilitate replication of primary HIV-1 strains in macrophages and formation of macrophage internal virus-containing compartments

  Sabina Victoria Montero, et al., bioRxiv - Microbiology 2024

  Quote: ... cells were stained with primary anti-HIV-Gag antibody (Beckman-Coulter; KC57-FITC, mouse derived), and cells were acquired using the MACs Quant Flow Cytometer (Milentyi ...
• The HIV latency reversal agent HODHBt enhances NK Cell effector and memory-like functions by increasing IL-15 mediated-STAT activation

  Amanda B. Macedo, et al., bioRxiv - Cell Biology 2021

  Quote: ... and intracellular staining with p24/FITC (clone KC57-FITC, catalog 6604665, Beckman Coulter).
• Molecular evolution of classic Hodgkin lymphoma revealed through whole genome sequencing of Hodgkin and Reed Sternberg cells

  Francesco Maura, et al., bioRxiv - Cancer Biology 2021

  Quote: ... The cells were stained with an antibody cocktail composed of CD64 FITC (22; Beckman Coulter (BC), Brea ...
• Immunophenotyping of T cells Combined with Vβ antibodies identifies long lasting CMV related T cell Expansions with a consistent TIGIT and PD-1 phenotype

  Monique M. van Ostaijen-ten Dam, et al., bioRxiv - Immunology 2024

  Quote: ... each including three distinct FITC and/or PE conjugated Vβ antibodies (Beckman Coulter, Brea, CA, USA) and a backbone panel of surface markers ...
• Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteins

  Fengwen Zhang, et al., bioRxiv - Microbiology 2022

  Quote: ... Gag KC57-FITC (Beckman Coulter) and Fixable Aqua Viability Dye (Invitrogen) ...
• The age and mouse sperm quality – a flow cytometry investigation

  Federica Zacchini, et al., bioRxiv - Developmental Biology 2021

  Quote: AnnexinV/FITC Kit (Beckman Coulter) was used to quantify apoptosis in mouse sperm ...
• Single cell resolution analysis of multi-tissue derived human iNKT cells reveals novel transcriptional paradigms

  Reyka G. Jayasinghe, et al., bioRxiv - Immunology 2024

  Quote: ... FITC Vα24 (Beckman Coulter, C15), PE Vβ11 (Beckman Coulter ...
• Epstein Barr virus infection induces tissue-resident memory T cells in mucosal lymphoid tissues

  Daniel Kirchmeier, et al., bioRxiv - Immunology 2023

  Quote: ... VB16 (TAMAYA1.2, Beckman Coulter, FITC), VB18 (BA62.6 ...
• Epstein Barr virus infection induces tissue-resident memory T cells in mucosal lymphoid tissues

  Daniel Kirchmeier, et al., bioRxiv - Immunology 2023

  Quote: ... VB8 (56C5.2, Beckman Coulter, FITC), VB11 (RE559 ...
• Epstein Barr virus infection induces tissue-resident memory T cells in mucosal lymphoid tissues

  Daniel Kirchmeier, et al., bioRxiv - Immunology 2023

  Quote: ... VB22 (IMMU546, Beckman Coulter, FITC), VB23 (AF23 ...
• Epstein Barr virus infection induces tissue-resident memory T cells in mucosal lymphoid tissues

  Daniel Kirchmeier, et al., bioRxiv - Immunology 2023

  Quote: ... VB13.6 (JU74.33, Beckman Coulter, FITC), VB14 (CAS1.1.3 ...
• Epstein Barr virus infection induces tissue-resident memory T cells in mucosal lymphoid tissues

  Daniel Kirchmeier, et al., bioRxiv - Immunology 2023

  Quote: ... VB17 (E17.5F3.15.13, Beckman Coulter, FITC)
• Epstein Barr virus infection induces tissue-resident memory T cells in mucosal lymphoid tissues

  Daniel Kirchmeier, et al., bioRxiv - Immunology 2023

  Quote: ... VB21.3 (IG125, Beckman Coulter, FITC), VB22 (IMMU546 ...
• Epstein Barr virus infection induces tissue-resident memory T cells in mucosal lymphoid tissues

  Daniel Kirchmeier, et al., bioRxiv - Immunology 2023

  Quote: ... VB5.2 (36213, Beckman Coulter, FITC), VB5.3 (3D11 ...
• HIV-1 cell-to-cell spread overcomes the virus entry block of non-macrophage-tropic strains in macrophages

  Mingyu Han, et al., bioRxiv - Microbiology 2022

  Quote: ... The following antibodies were used: RD1- or fluorescein isothiocyanate (FITC)-conjugated anti-Gag (clone KC57; Beckman Coulter), Alexa Fluor 647- or Fluor 555-conjugated phalloidin (Life Technologies) ...
• Lineage plasticity in SCLC generates non-neuroendocrine cells primed for vasculogenic mimicry

  Sarah M Pearsall, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Fixed cells were incubated with integrin β1-FITC (CD29-FITC, Beckman Coulter, IM0791U, 1:25) and Tomato Lectin Biotinylated (1:250 ...
• Multilevel human secondary lymphoid immune system compartmentalization revealed by complementary multiplexing and mass spectrometry imaging approaches

  Benjamin L. Oyler, et al., bioRxiv - Immunology 2022

  Quote: ... CD19-FITC (J3-119, Beckman-Coulter), CD27-BV605 (O323 ...
• Multimodal Mapping of Human Lymphopoiesis Reveals B and T/NK/ILC Lineages are Subjected to Cell-Intrinsic versus Flt3L-Dependent Regulation

  Kutaiba Alhaj Hussen, et al., bioRxiv - Immunology 2022

  Quote: ... CD7 FITC (Beckman Coulter, clone 8H8.1), CD123 BV786 (BD Bioscience ...
• Distinct Subsets of Multi-Lymphoid Progenitors Support Ontogeny-Related Changes in Human Lymphopoiesis

  Seydou Keita, et al., bioRxiv - Immunology 2022

  Quote: ... CD7 FITC (Beckman Coulter, clone 8H8.1), CD10 BV650 (BD bioscience ...
• Multimodal Mapping of Human Lymphopoiesis Reveals B and T/NK/ILC Lineages are Subjected to Cell-Intrinsic versus Flt3L-Dependent Regulation

  Kutaiba Alhaj Hussen, et al., bioRxiv - Immunology 2022

  Quote: ... CD7 FITC (Beckman Coulter, clone 8H8.1), CD38 PerCPCy5.5 (Biolegend ...
• Distinct Subsets of Multi-Lymphoid Progenitors Support Ontogeny-Related Changes in Human Lymphopoiesis

  Seydou Keita, et al., bioRxiv - Immunology 2022

  Quote: ... CD7 FITC (Beckman Coulter, clone 8H8.1), CD56 PC7 (Biolegend ...
• Distinct Subsets of Multi-Lymphoid Progenitors Support Ontogeny-Related Changes in Human Lymphopoiesis

  Seydou Keita, et al., bioRxiv - Immunology 2022

  Quote: ... CD7 FITC (Beckman Coulter, clone 8H8.1), CD10 BV650 (BD bioscience ...
• Distinct Subsets of Multi-Lymphoid Progenitors Support Ontogeny-Related Changes in Human Lymphopoiesis

  Seydou Keita, et al., bioRxiv - Immunology 2022

  Quote: ... CD7 FITC (Beckman Coulter, clone 8H8.1), CD123 BV786 (BD Bioscience ...
• Distinct Subsets of Multi-Lymphoid Progenitors Support Ontogeny-Related Changes in Human Lymphopoiesis

  Seydou Keita, et al., bioRxiv - Immunology 2022

  Quote: ... CD7 FITC (Beckman Coulter, clone 8H8.1), CD38 PerCPCy5.5 (Biolegend ...
• The CLIC/GEEC pathway regulates particle endocytosis and formation of the virus-containing compartment (VCC) in HIV-1-infected macrophages

  Kathleen Candor, et al., bioRxiv - Microbiology 2024

  Quote: ... FITC-KC57 was obtained from Beckman Coulter (Cat ...
• Expression of Intron-containing HIV-1 RNA Induces NLRP1 Inflammasome Activation in Myeloid Cells

  Sallieu Jalloh, et al., bioRxiv - Microbiology 2024

  Quote: ... Intracellular p24Gag was stained using FITC-conjugated mouse anti-p24Gag monoclonal antibody (KC57; Beckman Coulter, catalog # 6604665, 1:25) as previously described [88] ...
• Enhanced anti-tumor effects through continuous administration of engineered CAR-macrophages derived from pluripotent stem cell-derived myeloid cell lines

  Yuya Atsumi, et al., bioRxiv - Bioengineering 2024

  Quote: ... The following antibodies were used in this study flow cytometry analysis: CD36-FITC (#IM0766U; Beckman Coulter, Brea, CA, USA), CD45-FITC (#368508 ...
• The endocannabinoid 2-arachidonoylglycerol is released and transported on demand via extracellular microvesicles

  Verena M. Straub, et al., bioRxiv - Neuroscience 2024

  Quote: ... Membranes and cytosolic fractions were separated by centrifugation in a Beckman Optima LE-80K ultracentrifuge (Beckman Coulter Inc., Fullerton, CA, USA) at 100,000 g for 20 min at 4°C ...
• Release of P-TEFb from the Super Elongation Complex promotes HIV-1 latency reversal

  William J. Cisneros, et al., bioRxiv - Microbiology 2024

  Quote: Intracellular HIV-1 p24 staining was performed on fixed U1 and ACH-2 cells with KC57-FITC (Beckman Coulter, Cat#6604665). After allowing at least 30 minutes for fixation ...
• A dynamic compositional equilibrium governs mRNA recognition by eIF3

  Nicholas A. Ide, et al., bioRxiv - Biophysics 2024

  Quote: ... The subunits were then separated by ultracentrifugation in an SW32 rotor (Beckman Coulter, Catalog Number 369694) at 22,000 rpm for 17 hours at 4 °C.
• Compartment-driven imprinting of intestinal CD4 (regulatory) T cells in inflammatory bowel disease and homeostasis

  Lisanne Lutter, et al., bioRxiv - Immunology 2022

  Quote: ... TCRγδ FITC (clone IMMU510, IM1571U, Beckman Coulter), CD45RA APC-Cy7 (clone HI100 ...
• Therapy-induced senescence upregulates antigen presentation machinery and triggers anti-tumor immunity in Acute Myeloid Leukemia

  Diego Gilioli, et al., bioRxiv - Cancer Biology 2022

  Quote: ... CD33-FITC (cat#IM1135U from Beckman Coulter), CD34-PE (cat#130-113-179 from Miltenyi Biotech) ...
• Natural killer cell function is regulated by TGF-β signaling in pregnancy and tumor progression

  Adam Yalin, et al., bioRxiv - Immunology 2024

  Quote: ... CD3 (FITC, Beckman Coulter, cat. No. A07746), CD19 (Beckman Coulter ...
• P4HA2: A link between tumor-intrinsic hypoxia, partial EMT and collective migration

  Vaishali Aggarwal, et al., bioRxiv - Cancer Biology 2022

  Quote: ... The cell pellet was disaggregated and incubated with 2 μL of anti-pan cytokeratin-FITC (Beckman Coulter, Cat. No. IM2356U, Clone J1B3). A separate tube containing cells unstained for surface E-Cadherin was incubated after permeabilization with E-Cadherin antibody- (R&D Cat ...
• Dysregulated functional and metabolic response in multiple sclerosis patient macrophages correlate with a more inflammatory state, reminiscent of trained immunity

  J. Fransson, et al., bioRxiv - Neuroscience 2021

  Quote: ... Labeling was done with anti-HLA-DR-PB and anti-CD16-FITC (Duraclone pre-coated tubes or separate antibodies, all from Beckman-Coulter) and anti-CD14-PEvio770 (Miltenyi ...
• Cone-shaped HIV-1 capsids are transported through intact nuclear pores

  Vojtech Zila, et al., bioRxiv - Microbiology 2020

  Quote: ... cells were fixed with 4% FA in PBS for 90 min at room temperature and immunostained for intracellular HIV-1 CA for 30 min at 4°C using KC57-FITC antibody (Beckman Coulter) diluted in 0.1% Triton X-100 ...
• Cryo-EM structures of the human G-protein coupled receptor 1 (GPR1) – Gi protein complexes bound to the full-length chemerin and to its C-terminal nonapeptide

  Aijun Liu, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... The FITC fluorescence signals demonstrating the antibody-receptor complex on the cell surface were quantified by flow cytometry (CytoFLEX, Beckman Coulter). Relative expression levels of GPR1 mutants were represented according to the fluorescence signals.