Labshake search
Citations for Beckman :
451 - 500 of 1164 citations for Cis Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%; 1 D 97% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2024Quote: ... Target sequences were then amplified using specific primers (Table 1) and purified using AMPure beads (Beckman Coulter; A63880) at concentrations appropriate to each of the target sizes ...
-
bioRxiv - Cancer Biology 2021Quote: ... 0.03 mg/ml) and RNAsi A (0.2 mg/ml) using a flow cytofluorimeter Epics XL apparatus (Beckman Coulter). Cell aggregates were gated out on bi-parametric graph FL-3lin/ratio as described in (Ferrara et al. ...
-
bioRxiv - Biophysics 2023Quote: ... Samples were indexed using the IDT for Illumina Nextera DNA Unique Dual Indexes Set D (96 Indexes) and SPRISelect beads (Beckman Coulter) at a 0.9× ratio were used for cleanup and final size selection ...
-
bioRxiv - Immunology 2023Quote: Koff rates were determined as dissociation of reversible pMHC-Streptamers upon addition of D-Biotin in a flow cytometry-based assay (CyAn ADP Lx 9 color flow cytometer, Beckman Coulter) as described previously (44) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 70 ml polycarbonate bottles (Beckman Coulter) in a Sorvall™ WX Ultra Series ultracentrifuge (Thermo Fisher) ...
-
bioRxiv - Cell Biology 2019Quote: ... 48 and 72 hours then concentrated by ultracentrifugation (Beckman Coulter Optima XL-100) for 1 hour at 25K in an SW28 rotor and resuspended in PBS.
-
Multimodal tubulin binding by the yeast kinesin-8, Kip3, underlies its motility and depolymerizationbioRxiv - Biophysics 2021Quote: ... A final spin at 30,000 x g in a TLA 100 rotor (Beckman) for 10 min at 4 °C was carried out to remove big aggregates.
-
bioRxiv - Biochemistry 2022Quote: ... The mixture was clarified by centrifugation at 90,000 rpm (TLA-100; Beckman Coulter) for 5 minutes at 4 °C ...
-
bioRxiv - Cell Biology 2019Quote: ... The sample was spun in a TLA-100 rotor (Beckman Coulter, Brea, CA) at 80,000 rpm for 30 minutes at 4°C and gel filtered on a HiLoad Superdex 75pg column (GE-Healthcare ...
-
bioRxiv - Biophysics 2023Quote: ... This mixture was then spun down in a TLA-100 rotor (Beckman Coulter) at 9,892 g for 30 min at 4°C to sediment actin filament bundles into a low-speed pellet ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were fixed and lysed in 100 µL of OptiLyse B (Beckman Coulter) for 10 min at RT ...
-
bioRxiv - Molecular Biology 2024Quote: ... samples were cleaned up by adding 100 ul of SPRIselect beads (Beckman Coulter) and following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... and 500 μl of a 10 ml resuspension of cells from each flask diluted with 19.5 ml Isoton II Diluent (Beckman). PD time ...
-
bioRxiv - Biochemistry 2021Quote: Samples of filtered 0.5 mL plasma or CSF were added to 3.5 mL Open-Top Thickwall Polycarbonate ultracentrifuge tubes (Beckman Coulter), and PBS was added to fill tubes to the top ...
-
bioRxiv - Microbiology 2022Quote: ... Fractions were diluted in 5 ml PBS and washed by ultracentrifugation at 200,000 × g (60 min, 4°C, AH-650 rotor, 5 ml Beckman Ultra-Clear tubes ...
-
bioRxiv - Microbiology 2022Quote: CsCl linear gradients were prepared by overlaying 8.5 mL of 1.46 g/cm3 CsCl solution with 8.5 mL of 1.2 g/cm3 CsCl solution in 17 mL Ultra-Clear tubes (Beckman Coulter), which were then spun at a 45-degree angle and a speed of 20 rpm for 13.5 minutes using Gradient Master (BioComp).
-
bioRxiv - Plant Biology 2022Quote: ... was gently layered on top of 4.4 mL sucrose gradient solution (15 to 55%) in a 5.1 mL Quick-Seal polypropylene tube (Beckman Coulter) and centrifuged at 45,000 rpm for 65 min at 4°C (Sorval NVT 90 ...
-
bioRxiv - Neuroscience 2022Quote: ... myelin was layered onto 3 mL ice cold 0.85 M sucrose solution in a 10 mL ultracentrifuge tube (Beckman) before centrifugation at 75 000 x g for 30 min at 4 °C on a Beckman Ultracentrifuge with MLA-55 rotor (acceleration 7 ...
-
bioRxiv - Cell Biology 2022Quote: ... and 3.5 mL of 5% iodixanol in a 16.8 mL open top polyallomer tube (Beckman Coulter, Fullerton, California, USA). One milliliter of concentrated conditioned medium was pipetted on top of the gradient and samples were centrifuged for 18 h at 100,000 ×g and 4 °C using a SW 32.1 Ti rotor (Beckman Coulter ...
-
bioRxiv - Immunology 2023Quote: ... Layers of 2.1 mL of each solution were successively poured into 12.5 mL polyallomer tubes (Beckman-Coulter, Cat. # 331372), starting from the most concentrated solution (50 % ...
-
bioRxiv - Cell Biology 2024Quote: ... 1.6 ml of each fraction was very slowly layered in a 14 ml ultracentrifuge tube (Beckman Coulter, California, USA), starting from higher to lower concentrations ...
-
bioRxiv - Immunology 2019Quote: ... To identify KIR2DS1 negative donors, NK cells were stained with mAbs to KIR2DL1 (R&D, 143211) and KIR2DL1/S1 (Beckman Coulter, EB6) as described (Fauriat et al. ...
-
bioRxiv - Immunology 2021Quote: ... SSM liquid culture was incubated for 24 hours at 36°C with constant shaking at 180 rpm until reaching OD600nm 0.5 with 1 cm path width (Beckman Coulter™ DU 530 UV Vis spectrophotometer) ...
-
bioRxiv - Genomics 2020Quote: ... The sequencing libraries were cleaned up using AMPure XP beads (1:1.8 sample vol. to beads vol.; Beckman Coulter). The size distribution of the libraries were determined by Bioanalyzer (Agilent Technologies) ...
-
CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... diluted 5-fold in buffer A supplemented with 1 mM phenylmethane sulfonyl fluoride (PMSF) and centrifuged 40,000 rpm (45 Ti rotor, Beckman) for 60 min at 4°C to pellet vesicles ...
-
bioRxiv - Biochemistry 2021Quote: ... and resuspended in 500 μL of 1% FBS/DPBS and transferred to polystyrene tubes for flow cytometric analysis (Beckman Coulter HyperCyAn ...
-
bioRxiv - Biophysics 2020Quote: ... and centrifuged at 287,582 x g (90,000 rpm) for 1 hr at 4 °C in a TLA100.2 rotor (Beckman, ref. 362046) in 11×34 mm thick-wall polycarbonate ultracentrifuge tubes (Beckman ...
-
bioRxiv - Neuroscience 2022Quote: ... The samples were separated through ultracentrifugation for 1 hr at 200,000 g (spin 3 at 49,000 r.p.m. in Beckman TLS-55 rotor). Top layers of supernatants were removed ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with the fluorescently labeled anti-p24 antibody (Clone: KC57, Beckman Coulter, 6604667; 1/200 dilution).
-
bioRxiv - Biochemistry 2022Quote: ... 1 mM DTT using a MLA-50 rotor in an Optima MAX-XP Ultracentrifuge (Beckman Coulter, Pasadena, California, USA) at 180.000 g for 5 h at 4 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... The crude extract was centrifuged at 200,000 g for 1 h at 4 °C (Beckman Coulter Optima LE-80K). The membrane pellet was homogenized and solubilized in 20 mM HEPES (pH 7.8 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Amplicons from up to 3,072 libraries were pooled and purified with Ampure XP beads (0.8:1 ratio beads to product; Beckman Coulter). These steps were performed using Biomek FxP (Beckman Coulter) ...
-
bioRxiv - Microbiology 2021Quote: ... buoyant density of 1.71 g ml−1) in polyallomer tubes before sealing and ultracentrifugation at 152,000 × g (50,000 rpm) in a MLN80 rotor (Beckman-Coulter) for 72 h at 25°C ...
-
bioRxiv - Developmental Biology 2019Quote: ... Cells were refixed in 1% PFA/PBS and analyzed using a CyAn ADP Flow Cytometer (Beckman Coulter, Miami, FL). Data were collected using a four-decade log amplifier and stored in list mode for subsequent analysis using FlowJo Software (Tree Star ...
-
bioRxiv - Microbiology 2019Quote: ... The concentrated vesicles were washed twice with PBS by ultracentrifugation at 110,000xg at 4°C for 1 hour (Beckman Optma MAX-XP Ultracentrifuge ...
-
bioRxiv - Genomics 2019Quote: ... All extractions from each respective fragment section were then pooled into two samples (pooled sample 5_27-10 and pooled sample 6_2-1) and re-purified using AMPure XP Beads (Agencourt, Beckman Coulter) adding 0.4 volume (V/V ...
-
bioRxiv - Biochemistry 2021Quote: ... Reaction mixtures were incubated for 30 minutes and centrifuged at 100,000g at 4°C for 1 hr using a TLA100 fixed angle rotor (Beckman). The supernatant fraction was carefully removed ...
-
bioRxiv - Molecular Biology 2019Quote: After depletion of the gene of interest samples were purified using a 1 X volume XP beads (Beckman Coulter), quantified and subsequently subjected to 75-bp-single-end sequencing on the Illumina Nextseq 500 platform.
-
bioRxiv - Neuroscience 2019Quote: ... Then the lysates were spin down at 67,000 rpm for 1 hour at 18 °C with the ultracentrifuge (Beckman). The AAV particles were collected by aspirating the interface between 40-60% iodixanol with a syringe ...
-
bioRxiv - Immunology 2020Quote: ... 10 mM Tris-HCl, pH 8.1), chromatin was eluted (1% SDS, 0.1M NaHCO3)and recovered with Agencourt AMPure XP beads (Beckman Coulter). Bound chromatin and input DNA were treated with RNase H (New England Biolabs ...
-
bioRxiv - Biochemistry 2019Quote: ... and centrifuged at 287,582 x g (90,000 rpm) for 1 hr at 4°C in a TLA100.2 rotor (Beckman, ref. 362046) in 11×34 mm thick-wall polycarbonate ultracentrifuge tubes (Beckman ...
-
bioRxiv - Cell Biology 2019Quote: ... F-actin was pelleted by ultracentrifugation for 1 h at room temperature at 45,000 rpm (Beckman TLA-55 rotor) and re-suspended in ice cold G-buffer ...
-
bioRxiv - Cancer Biology 2022Quote: ... resuspended in 250 µl FACS buffer with 1 µl DAPI and analyzed with a Gallios flow cytometer (Beckman Coulter)
-
bioRxiv - Microbiology 2022Quote: ... the cross-linked material was loaded onto glycerol-tartrate gradients as described elsewhere 53 and centrifuged for 1 h at 25,000 rpm in an SW-40 rotor (Beckman), with brakes set at the slowest possible deceleration ...
-
bioRxiv - Microbiology 2022Quote: ... The post-nuclear supernatant was adjusted to 0.8 M sucrose and loaded in the middle of a step gradient ranging from 0.1 to 1.8 M sucrose in STKM buffer and centrifuged at 50’000 g for 1 h at 4°C in a TLA-120.1 fixed-angle rotor (Beckman Coulter). LDs formed a white cushion on top of the tube and were collected by aspiration of the upper fraction of the gradient (125 µl).
-
bioRxiv - Neuroscience 2022Quote: ... Then the lysates were spin down at 67,000 rpm for 1 hour at 18 °C with the ultracentrifuge (Beckman). The AAV particles were collected by collecting the interface between 40 and 60% iodixanol with a syringe ...
-
Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... Individual compounds (1 µM in 0.1% DMSO) were added to each well using a Biomek NX workstation (Beckman-Coulter)(1 well per compound) ...
-
bioRxiv - Biophysics 2023Quote: ... The supernatant was subjected to a second round of ultracentrifugation at 100,000 x g for 1 hour at 4°C in a Type 45Ti rotor (Beckman) to pellet cell membranes ...
-
bioRxiv - Plant Biology 2023Quote: ... It was then centrifuged again at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter ...
-
bioRxiv - Cell Biology 2023Quote: ... and 1 mM PMSF) and centrifuged for 15 hours at 4°C with 74,400 xg (SW 32.1 Ti; Beckman Coulter). 24 equal volume fractions were collected from the top and subjected to TCA precipitation ...